FastQCFastQC Report
Wed 25 May 2016
SRR1780415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287781
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTTT250.002352099935.1930430
CTCAATA250.002360144435.1685632
TAACACG353.2418827E-431.378684
CCGATAC300.00571411629.35818339
GAATATC300.00571411629.35818336
TCAGCGA300.00571411629.35818337
TTAATAC454.098271E-529.2918552
GCTGTCC300.005782557729.2867648
GGAATCT406.94884E-427.54728915
GTATACC407.070131E-427.4658851
CGGTGGT1450.027.28120843
CTATACA1400.026.6811491
TAATACG508.410164E-526.3580884
CCGTGTA701.1206575E-625.15977534
CTTTACA1750.025.1116681
CATACCA450.00138593624.47367919
CGATACG450.001388753324.46515340
CCGAATG450.001397233524.43961129
ATCTCGT450.001402910624.42261141
GTACAAG450.001405756224.4141221
Produced by FastQC (version 0.11.5)