FastQCFastQC Report
Wed 25 May 2016
SRR1780415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287781
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3161510.985784329055775No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT251288.731639684343302RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC78012.7107418488364416No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC44201.5358901386818449No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG38601.3412977229212493No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT26420.9180592186419534No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC18390.6390275938995277No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC13880.4823112019209051No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG12070.4194161532554269No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC7930.2755567601752722No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG7630.26513216647381166No Hit
CTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5850.20327957717847944No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5840.2029320907217641No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5410.1879901730830041No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5210.1810404439486971No Hit
CTTATCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC4680.16262366174278356No Hit
CTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT3520.12231523276380303No Hit
ACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCT3440.11953534111008024RNA PCR Primer, Index 32 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC2900.10077107244745136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCAGG207.840836E-444.00660341
CCGAAAT207.847551E-443.99895529
CGCCAGA207.847551E-443.99895531
CCGTCTT26200.042.0750244
CTATACA4700.039.7932051
CTTATAA1000.039.6059461
CTTTACA8600.038.3778531
TCTTTAT2200.038.0057031
GCCGTCT29200.037.7522443
TTTACAC8750.037.713392
CACATCG301.298853E-436.6657947
CGGGCGA301.298853E-436.66579412
CGAAATT250.002350097335.19916530
CGAAAGG250.002350097335.19916531
CATTATC250.002350097335.19916530
CACTACG250.002350097335.19916539
TTACACA9450.035.1526033
CTTATAC44400.032.5093841
TGACGAT353.2089915E-431.43328940
GACGATA353.2089915E-431.43328941
Produced by FastQC (version 0.11.5)