FastQCFastQC Report
Wed 25 May 2016
SRR1780414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24546
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC310.1262934897743013Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGAG150.002199952569.9918419
GAAACAC452.401248E-854.886151
GAAACCC651.5643309E-1048.8547021
GAAAACA501.5847055E-435.2839471
TCGGTGG450.004229868331.10748342
CGGTGGT450.004229868331.10748343
TCTCGGT500.007079649327.99673540
CTCGGTG500.007079649327.99673541
CGACGAA800.00259584421.87244819
AAAACCC900.00489820719.6021961
AACCCAA900.0051372219.4421773
AAACCCA1301.0058764E-418.8439562
AAACCCC1501.4029034E-518.664492
GCTGCCG1501.4029034E-518.6644914
CCGACGA1602.4454475E-517.4979618
CGCTGCC1704.11522E-516.46866813
ACGCTGC1704.11522E-516.46866812
CTGCCGA1503.0068235E-416.33142915
CTGACGC1755.2751337E-515.9981339
TGACGCT1858.4823165E-515.1333710
Produced by FastQC (version 0.11.5)