##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780414_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24546 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.73262446019718 21.0 12.0 32.0 12.0 34.0 2 22.51144789375051 23.0 12.0 33.0 11.0 34.0 3 23.018332925934978 23.0 11.0 33.0 11.0 34.0 4 23.439786523262445 23.0 11.0 34.0 11.0 34.0 5 23.40197995600098 23.0 11.0 34.0 11.0 34.0 6 25.59480159700155 27.0 11.0 36.0 11.0 38.0 7 25.46716369265868 27.0 11.0 36.0 11.0 38.0 8 25.216002607349466 27.0 11.0 36.0 11.0 38.0 9 25.19750672207284 27.0 12.0 36.0 12.0 38.0 10-11 24.227735679947852 26.0 11.0 35.5 11.0 38.0 12-13 24.10335696243787 26.0 11.0 35.5 11.0 38.0 14-15 23.859223498737066 25.5 11.0 34.5 11.0 38.0 16-17 23.267762568239228 23.5 11.0 34.0 11.0 38.0 18-19 23.630326733479997 24.5 11.0 34.0 11.0 38.0 20-21 23.732970748798174 24.5 11.0 34.0 11.0 38.0 22-23 23.15383361851218 23.0 11.0 34.0 11.0 38.0 24-25 23.337325837203615 23.5 11.0 34.0 11.0 38.0 26-27 23.23851136641408 23.0 11.0 34.0 11.0 38.0 28-29 22.716552595127517 21.5 11.0 34.0 11.0 38.0 30-31 22.6899902224395 21.5 11.0 34.0 11.0 38.0 32-33 22.92831825959423 22.5 11.0 34.0 11.0 38.0 34-35 23.076203862136396 23.0 11.0 34.0 11.0 38.0 36-37 22.923694288275076 23.0 11.0 34.0 11.0 38.0 38-39 22.706754664711156 21.0 11.0 34.0 11.0 38.0 40-41 22.755214698932615 22.5 11.0 34.0 11.0 38.0 42-43 23.048582253727695 22.5 11.0 34.0 11.0 38.0 44-45 22.651633667399985 22.0 11.0 34.0 11.0 38.0 46-47 22.97095249735191 22.5 11.0 34.0 11.0 38.0 48-49 23.20610282734458 23.5 11.0 34.0 11.0 38.0 50-51 23.213293408294632 23.5 11.0 34.0 11.0 38.0 52-53 23.102216247046364 23.0 11.0 34.0 11.0 38.0 54-55 23.05389880224884 23.0 11.0 34.0 11.0 38.0 56-57 22.63124337977675 22.0 11.0 34.0 11.0 38.0 58-59 22.55398028191966 22.0 11.0 34.0 11.0 38.0 60-61 22.829137130285993 23.0 11.0 34.0 11.0 38.0 62-63 22.53998614845596 22.5 11.0 33.5 11.0 38.0 64-65 22.705797278578995 22.5 11.0 34.0 11.0 38.0 66-67 23.003992503870286 23.0 11.0 34.0 11.0 38.0 68-69 22.927971970993237 23.5 11.0 33.5 11.0 38.0 70-71 23.044345310844946 23.5 11.0 34.0 11.0 37.5 72-73 22.872097286726962 23.5 11.0 34.0 11.0 38.0 74-75 22.266845921942476 22.5 11.0 33.0 11.0 37.0 76 21.192903120671392 20.0 11.0 32.0 11.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02544179962217541 1101 2 -0.6709512215171145 1101 3 0.2207280469003976 1101 4 -1.011012106939642 1101 5 0.8416502986759689 1101 6 -1.3222439425177797 1101 7 -1.0745091624152927 1101 8 -2.358458626728744 1101 9 -0.0911805499808942 1101 10-11 -0.06338175284748004 1101 12-13 0.13114647086776898 1101 14-15 0.4839413351878612 1101 16-17 0.4555642231281425 1101 18-19 -1.3581850215408977 1101 20-21 -1.1264227440401022 1101 22-23 -1.1258357756183628 1101 24-25 -0.5147498696135457 1101 26-27 1.4680795828666753 1101 28-29 -0.3243528231386428 1101 30-31 -0.054803799663957875 1101 32-33 -0.7510811103858188 1101 34-35 -1.5795711012262217 1101 36-37 0.13017007490088872 1101 38-39 0.45658546070737316 1101 40-41 1.0352464244082853 1101 42-43 -1.4202193236639467 1101 44-45 1.9570860427737884 1101 46-47 0.052474698975874645 1101 48-49 -2.429004419285107 1101 50-51 -0.1392155228566807 1101 52-53 -2.0823135682620553 1101 54-55 0.38863516229124073 1101 56-57 -2.352030235627904 1101 58-59 -1.4079310218219803 1101 60-61 -0.9180227508284666 1101 62-63 -0.001315957212781882 1101 64-65 -0.787477314457842 1101 66-67 -0.815376489975165 1101 68-69 -2.0543906214157346 1101 70-71 -0.07446620902897294 1101 72-73 -0.7936846285112615 1101 74-75 -0.3769030054753415 1101 76 -0.017856817700923955 1102 1 -0.8716170239072376 1102 2 -1.8327159273994695 1102 3 -1.1910366589819539 1102 4 -1.231600342233758 1102 5 -0.17305558367697316 1102 6 0.3983442927763363 1102 7 -1.3686268094741187 1102 8 -0.4172821561405087 1102 9 0.24705474413675077 1102 10-11 -1.18838175284748 1102 12-13 -1.979147646779289 1102 14-15 -2.0087057236356642 1102 16-17 -3.1106122474600895 1102 18-19 -1.6155379627173687 1102 20-21 -1.2808345087459863 1102 22-23 -1.4199534226771853 1102 24-25 -1.3382792813782523 1102 26-27 -3.2598615936039153 1102 28-29 -1.3096469407857008 1102 30-31 -2.260686152605132 1102 32-33 -2.052551698621116 1102 34-35 -0.9178063953438667 1102 36-37 -1.6124769839226403 1102 38-39 -1.4184145392926268 1102 40-41 -1.7441653402975952 1102 42-43 -1.1481605001345336 1102 44-45 -2.6237963101673927 1102 46-47 -0.10193706573000227 1102 48-49 -0.4657691251674585 1102 50-51 -2.1245096405037387 1102 52-53 -1.4279018035561712 1102 54-55 -1.4275413082969912 1102 56-57 -0.7417361179808424 1102 58-59 -1.2167545512337483 1102 60-61 -1.2341992214167021 1102 62-63 -2.2807277219186624 1102 64-65 -0.3757126085754905 1102 66-67 -0.896258842916339 1102 68-69 -3.8632141508274955 1102 70-71 -1.5156426796172084 1102 72-73 -0.47750815792302603 1102 74-75 -1.193079476063577 1102 76 -2.0619744647597464 1103 1 -1.5725974160640988 1103 2 -1.3147420711903202 1103 3 1.2632117070311182 1103 4 0.5878441022106848 1103 5 1.4625653313557052 1103 6 0.1148475607501922 1103 7 -0.1692804042453595 1103 8 -0.6623801953561923 1103 9 0.45947304479034656 1103 10-11 -0.03151900774943783 1103 12-13 0.40688176498541395 1103 14-15 0.14652303453426896 1103 16-17 -0.2723769533424445 1103 18-19 0.9490045209427649 1103 20-21 -0.5091841819486014 1103 22-23 0.48854330934895884 1103 24-25 -0.6597662094828252 1103 26-27 -0.2165609400091455 1103 28-29 -0.4330129538575953 1103 30-31 -0.6659149107750686 1103 32-33 -2.7678294763988944 1103 34-35 -0.3961560685464818 1103 36-37 0.519058963789778 1103 38-39 -0.5740517941945882 1103 40-41 -0.5231686082714511 1103 42-43 -1.1126212844482595 1103 44-45 -2.8304956565726194 1103 46-47 -2.5909076539652993 1103 48-49 0.5191164957475713 1103 50-51 -0.38676454246451897 1103 52-53 0.07209819644382875 1103 54-55 0.5083247047749033 1103 56-57 -0.9104452683076403 1103 58-59 -0.8601369041749223 1103 60-61 -1.5740685024624526 1103 62-63 -0.7905316434872951 1103 64-65 -2.109781236026471 1103 66-67 -0.6625987121973864 1103 68-69 -3.2598644776248804 1103 70-71 -1.2746296077217814 1103 72-73 -0.5404166546550542 1103 74-75 0.5618224847207358 1103 76 -0.3446542033218378 1104 1 -0.2392640827307666 1104 2 -0.285502305108448 1104 3 -0.3961450181150816 1104 4 0.0973470261872933 1104 5 -0.008194902562422612 1104 6 -0.7967021468521445 1104 7 0.18013480043300234 1104 8 -0.4737837041281239 1104 9 0.2942683664277723 1104 10-11 0.6154108168119663 1104 12-13 -0.4450919192251135 1104 14-15 -1.0369564976294718 1104 16-17 -0.6671137954477047 1104 18-19 -1.0195627305192225 1104 20-21 -1.3450759948140956 1104 22-23 -0.9156964567329133 1104 24-25 -1.0789913556816586 1104 26-27 -0.9672919341611887 1104 28-29 -0.7276035971324468 1104 30-31 -0.975856431242903 1104 32-33 -0.6775516986211159 1104 34-35 -0.982047881411976 1104 36-37 0.5775384959535188 1104 38-39 -0.015937759106865457 1104 40-41 -0.358329426984902 1104 42-43 -1.2847704072552766 1104 44-45 -0.9167529665141387 1104 46-47 0.23475024077154316 1104 48-49 -0.8430911065916114 1104 50-51 0.44437580841267277 1104 52-53 -1.2568491719772226 1104 54-55 -0.4701109677397177 1104 56-57 -0.7785008238631974 1104 58-59 -0.9555316410170285 1104 60-61 0.694206351338714 1104 62-63 -1.016408836469747 1104 64-65 -0.13500053427208059 1104 66-67 -0.3680080689225349 1104 68-69 -1.94370950686465 1104 70-71 0.7896978776583339 1104 72-73 0.04803363774260916 1104 74-75 0.158313712790914 1104 76 -2.0186308115089737 1105 1 -1.1359307493974349 1105 2 -1.6125198489680947 1105 3 -0.8645660707466618 1105 4 -0.6316003422337566 1105 5 -2.668545779755405 1105 6 0.24540311630574863 1105 7 -1.4770581820231357 1105 8 -0.5157135286895276 1105 9 0.4739174892347897 1105 10-11 -0.40651900774943783 1105 12-13 0.49104843165208223 1105 14-15 -1.3779214099101758 1105 16-17 -1.134043620009109 1105 18-19 -0.18043992350168025 1105 20-21 -1.269461959726378 1105 22-23 -1.5686789128732634 1105 24-25 -1.8317106539272707 1105 26-27 -0.8854498288980324 1105 28-29 -0.3369018427464816 1105 30-31 -1.8214704663306236 1105 32-33 -1.0842183652877821 1105 34-35 -0.3364338463242582 1105 36-37 -1.0431632584324433 1105 38-39 -1.4165517941945858 1105 40-41 -0.5178908304936733 1105 42-43 -0.7234546177815915 1105 44-45 0.6356154545384882 1105 46-47 -1.716740987298632 1105 48-49 -1.2744946153635404 1105 50-51 -1.6117645424645204 1105 52-53 -0.7129018035561714 1105 54-55 -0.9969530730028744 1105 56-57 -0.9601674905298623 1105 58-59 -2.0309702375082566 1105 60-61 -1.3835129469068974 1105 62-63 -1.9905316434872944 1105 64-65 -2.237281236026469 1105 66-67 -2.355376489975164 1105 68-69 -1.7079200331804358 1105 70-71 -1.7240740521662268 1105 72-73 -0.7115277657661636 1105 74-75 -0.7781775152792605 1105 76 -1.7468764255440625 1106 1 -1.7539699650837086 1106 2 -0.876833574458292 1106 3 -0.9557425413348959 1106 4 -2.4080709304690515 1106 5 -1.4377614660299152 1106 6 0.32481488101163336 1106 7 -1.0009797506505862 1106 8 1.8768354909183174 1106 9 -1.8558864323338362 1106 10-11 -2.548675870494538 1106 12-13 -1.670324117367528 1106 14-15 -1.3616469001062548 1106 16-17 -1.7797298945189155 1106 18-19 -2.637596786246778 1106 20-21 -1.5087756852165732 1106 22-23 -1.6037769520889533 1106 24-25 -2.4706322225547197 1106 26-27 -2.4804498288980312 1106 28-29 -0.4567057643151138 1106 30-31 -1.5327449761345449 1106 32-33 -1.9349046397975869 1106 34-35 -0.3736887482850406 1106 36-37 -1.0904181603932273 1106 38-39 0.11835016658972464 1106 40-41 -1.2294594579446532 1106 42-43 -0.14816050013453363 1106 44-45 -0.5502668984026862 1106 46-47 -1.2269370657300058 1106 48-49 -1.421651478108636 1106 50-51 -0.5068625816802061 1106 52-53 -2.391137097673816 1106 54-55 -1.8246001318264042 1106 56-57 -1.6461478826867264 1106 58-59 -2.3049898453513933 1106 60-61 -1.572434515534347 1106 62-63 -1.8101394866245464 1106 64-65 -1.4124773144578384 1106 66-67 -1.1242000193869295 1106 68-69 -1.4955670920039665 1106 70-71 -1.206819150205444 1106 72-73 -0.5142728638053811 1106 74-75 -0.7519030054753415 1106 76 -2.003150935347982 1107 1 0.1626346514464494 1107 2 -0.2655156295588128 1107 3 0.20378835963308717 1107 4 0.3127034552345975 1107 5 0.1257158236201228 1107 6 0.6529980530146098 1107 7 1.1025198770485929 1107 8 1.2532316189897976 1107 9 0.921849978686268 1107 10-11 0.4436919627146949 1107 12-13 0.5659007523271882 1107 14-15 1.408829644942145 1107 16-17 0.7260407681765422 1107 18-19 1.1981254773422023 1107 20-21 1.1357701077841718 1107 22-23 -0.6508730057002694 1107 24-25 0.5141965190685092 1107 26-27 1.0148033356589323 1107 28-29 0.40014457075562504 1107 30-31 0.9072215167917363 1107 32-33 1.2021951368219206 1107 34-35 1.717152651566039 1107 36-37 1.2757396951540514 1107 38-39 0.5934060117125846 1107 40-41 1.2796619121223607 1107 42-43 1.584899812598156 1107 44-45 1.3454888722600096 1107 46-47 0.7801366498321691 1107 48-49 1.6007796462398396 1107 50-51 1.7498388330629027 1107 52-53 1.3774779432792705 1107 54-55 0.4164646485161114 1107 56-57 0.35915740398490215 1107 58-59 1.480844108483307 1107 60-61 0.3585967577344533 1107 62-63 0.3845738417447748 1107 64-65 1.491748299838509 1107 66-67 1.4947500923033132 1107 68-69 0.5746116123891838 1107 70-71 1.5314533739941112 1107 72-73 0.8126072553308816 1107 74-75 0.4204722737502742 1107 76 0.6377649246669073 1108 1 0.38406925060256825 1108 2 0.827480151031903 1108 3 1.522100595920005 1108 4 1.2750663244329097 1108 5 0.7181208869112616 1108 6 0.5387364496390816 1108 7 -0.03705818202313793 1108 8 0.657619804643808 1108 9 1.1405841559014576 1108 10-11 -0.33318567441610725 1108 12-13 0.49104843165208223 1108 14-15 -0.43792140991017803 1108 16-17 1.4459563799908892 1108 18-19 0.026226743164986743 1108 20-21 0.683871373606955 1108 22-23 1.5713210871267336 1108 24-25 -0.4983773205939386 1108 26-27 1.1878835044353018 1108 28-29 1.2697648239201804 1108 30-31 0.37186286700271154 1108 32-33 -0.22421836528778272 1108 34-35 -1.223100512990925 1108 36-37 0.39017007490088673 1108 38-39 -0.823218460861252 1108 40-41 -0.37789083049367633 1108 42-43 0.516545382218407 1108 44-45 0.4889487878718235 1108 46-47 -0.38340765396529974 1108 48-49 -0.03449461536353837 1108 50-51 -0.04509787579785396 1108 52-53 -0.25956847022283824 1108 54-55 -0.30361973966953926 1108 56-57 0.9664991761368 1108 58-59 0.175696429158414 1108 60-61 0.16982038642643715 1108 62-63 0.06946835651270789 1108 64-65 1.0827187639735314 1108 66-67 1.7179568433581665 1108 68-69 -0.21458669984710355 1108 70-71 0.15592594783377578 1108 72-73 0.9418055675671688 1108 74-75 -0.12484418194592806 1108 76 -0.6668764255440607 1109 1 1.8312487377820545 1109 2 0.9844032279549815 1109 3 1.7738954677148797 1109 4 -0.5264721371055536 1109 5 1.312992681783058 1109 6 -0.5186994477968163 1109 7 0.5080700231050699 1109 8 0.5171069841309865 1109 9 1.9744303097476106 1109 10-11 0.9396348384044053 1109 12-13 0.5046381752418228 1109 14-15 0.8366939747052093 1109 16-17 0.6667256107601176 1109 18-19 1.091098538036782 1109 20-21 -0.054590164854584344 1109 22-23 0.3079877537934017 1109 24-25 0.08008421786760067 1109 26-27 0.4394219659737644 1109 28-29 0.48899559315094976 1109 30-31 0.13109363623348003 1109 32-33 0.6237303526609352 1109 34-35 0.6353610254706155 1109 36-37 1.3801700749008887 1109 38-39 0.3570379493951563 1109 40-41 -0.1027626253654681 1109 42-43 0.5203915360645617 1109 44-45 0.30946160838464465 1109 46-47 -0.5444332949909416 1109 48-49 1.4608900000210774 1109 50-51 0.45618417548419643 1109 52-53 0.3765853759310076 1109 54-55 0.7609956449458437 1109 56-57 0.2311145607521894 1109 58-59 0.4972348906968733 1109 60-61 0.8949485915546411 1109 62-63 0.0492119462562961 1109 64-65 0.27964184089660904 1109 66-67 0.4730850484863751 1109 68-69 -0.46766362292402874 1109 70-71 -0.23509969319186652 1109 72-73 -0.38229699653539484 1109 74-75 0.4400276129258671 1109 76 -0.6437995024671395 1110 1 -0.2850387306180906 1110 2 -0.9141160930995511 1110 3 -0.3772421270846884 1110 4 0.585301066216946 1110 5 -0.35511854970845746 1110 6 0.2566707219395532 1110 7 -0.02334926183534236 1110 8 -0.08961024230455195 1110 9 -0.5174440131126268 1110 10-11 0.35169695469187445 1110 12-13 -0.17674499557796608 1110 14-15 -1.3730387808021938 1110 16-17 -0.5065318829199086 1110 18-19 -1.7988436793702256 1110 20-21 -0.5442976404775486 1110 22-23 -1.272998161699558 1110 24-25 -1.9208186351479277 1110 26-27 -1.3308019415740873 1110 28-29 -1.8330520774882686 1110 30-31 -0.9632545038893099 1110 32-33 -0.804312262001396 1110 34-35 -0.8045089636951488 1110 36-37 -1.8698299250991113 1110 38-39 -0.20227949372510423 1110 40-41 -0.356012896221376 1110 42-43 -0.0665532093308876 1110 44-45 0.33167179256665946 1110 46-47 -1.825942865232907 1110 48-49 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2116 54-55 0.16585394454098434 2116 56-57 1.311411456838556 2116 58-59 1.895345551965427 2116 60-61 2.567013368882577 2116 62-63 1.5866613389688489 2116 64-65 0.9220170095875666 2116 66-67 2.3337463170423796 2116 68-69 2.1176940019072816 2116 70-71 1.7962768250267551 2116 72-73 0.2519810061636605 2116 74-75 1.33375230928214 2116 76 2.915579714806814 2117 1 -1.39311023657692 2117 2 -0.3168788233270696 2117 3 -2.0337968399774304 2117 4 -2.1354464960799113 2117 5 -0.48187911308873765 2117 6 -0.7622891913865573 2117 7 0.5080700231050699 2117 8 0.9978762149002165 2117 9 0.8205841559014573 2117 10-11 0.23130150507107317 2117 12-13 -0.37677208116843275 2117 14-15 0.05464269265392829 2117 16-17 0.11223843127293875 2117 18-19 0.3250728970111396 2117 20-21 -0.39112862639304424 2117 22-23 -0.06701224620659829 2117 24-25 -0.4904286026452169 2117 26-27 -1.9227575212057246 2117 28-29 0.7486109777663366 2117 30-31 0.031734661874502024 2117 32-33 -1.341013237082656 2117 34-35 -1.826177436067848 2117 36-37 -0.11982992509911128 2117 38-39 -0.9698851275279203 2117 40-41 -0.24699339459623815 2117 42-43 -0.9219161562431317 2117 44-45 -0.03669223776920205 2117 46-47 -0.7399461155037592 2117 48-49 0.992941282072362 2117 50-51 0.731825201125222 2117 52-53 0.9951751195207521 2117 54-55 1.2257392346894314 2117 56-57 0.6253453299829559 2117 58-59 0.25044001890200107 2117 60-61 -0.6018462802402311 2117 62-63 -0.659121387077036 2117 64-65 -0.569717133462369 2117 66-67 -1.9019149515136249 2117 68-69 -0.1759969562573609 2117 70-71 0.3514387683465934 2117 72-73 -0.5617841760225737 2117 74-75 0.19323274113099487 2117 76 1.369021010353375 2118 1 0.2656378780535462 2118 2 0.6721860333848433 2118 3 -1.068487639374112 2118 4 0.31741926560938083 2118 5 -0.8348202895593246 2118 6 -0.9938125699687603 2118 7 0.5480398571925491 2118 8 1.004286471310472 2118 9 -0.14020015782403306 2118 10-11 -0.35749939990630253 2118 12-13 -0.12620647030870202 2118 14-15 0.22168643322708093 2118 16-17 0.9898779486183393 2118 18-19 -0.43416541369775885 2118 20-21 -0.16318744992245726 2118 22-23 0.13641912634242104 2118 24-25 -0.1054361441233489 2118 26-27 1.5514129162000074 2118 28-29 0.22466678470449608 2118 30-31 0.40598051406153246 2118 32-33 -0.7167673848956255 2118 34-35 0.253762232107114 2118 36-37 1.1889936043126532 2118 38-39 0.5105070293348248 2118 40-41 0.2558346597024048 2118 42-43 0.6459571469242924 2118 44-45 -0.4718355258536633 2118 46-47 -1.1190939284751025 2118 48-49 -0.4192004977164778 2118 50-51 -1.3745096405037387 2118 52-53 -0.24898023492871957 2118 54-55 0.5430469269971248 2118 56-57 0.8759109408426866 2118 58-59 -0.5525388649592351 2118 60-61 0.023153719759768876 2118 62-63 -0.41798262387944973 2118 64-65 -1.2482616281833323 2118 66-67 -0.11929805860261666 2118 68-69 0.5289427119176011 2118 70-71 -1.030348561970147 2118 72-73 0.21121733227305128 2118 74-75 1.218685229818778 2118 76 -0.22373917064210147 2119 1 2.16290983031271 2119 2 2.5234221800174126 2119 3 0.9093469727316013 2119 4 1.681443136027113 2119 5 1.7572513216938717 2119 6 2.1540987684796633 2119 7 -2.5303915153564702 2119 8 -1.3507859924576415 2119 9 0.8423232863362387 2119 10-11 0.6297128763085311 2119 12-13 -1.046922582840672 2119 14-15 -1.5298054678811894 2119 16-17 0.037405655353207834 2119 18-19 1.0353571779475956 2119 20-21 -0.07047645247999768 2119 22-23 0.8405964494455738 2119 24-25 0.45104296926113463 2119 26-27 -0.06197156802846848 2119 28-29 0.6654169978332263 2119 30-31 -0.08741249531612993 2119 32-33 -0.7210299594906786 2119 34-35 0.43385600874820796 2119 36-37 0.09756137924871666 2119 38-39 -0.5459720840496587 2119 40-41 -0.1303545986096175 2119 42-43 0.8882845126531898 2119 44-45 0.03605023714718669 2119 46-47 -0.44688591483486206 2119 48-49 0.4469546599987808 2119 50-51 0.415771689419536 2119 52-53 0.6210112399220904 2119 54-55 -2.2069530730028752 2119 56-57 -1.3056747369066741 2119 58-59 0.19149353060768703 2119 60-61 0.3166319806293316 2119 62-63 -0.40285048406700597 2119 64-65 1.0143129668720832 2119 66-67 -0.4784199682360324 2119 68-69 -0.5844417723108748 2119 70-71 0.03998391884826802 2119 72-73 0.8847041182918076 2119 74-75 -0.9091920080328819 2119 76 -2.1176010632252193 >>END_MODULE >>Per sequence quality scores fail #Quality Count 10 1.0 11 4.0 12 22.0 13 146.0 14 532.0 15 1183.0 16 1975.0 17 2575.0 18 2708.0 19 2593.0 20 2113.0 21 1571.0 22 1064.0 23 748.0 24 507.0 25 355.0 26 276.0 27 250.0 28 234.0 29 208.0 30 237.0 31 265.0 32 311.0 33 396.0 34 566.0 35 846.0 36 1294.0 37 1566.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.382029840957536 27.721757665191017 9.202328250532874 32.69388424331858 2 7.504277682718162 43.27385317363318 26.057198728917136 23.164670414731525 3 6.840218365517803 47.30709687932861 21.482115212254545 24.370569542899045 4 4.929520084738857 47.893750509247944 20.748798174855374 26.427931231157824 5 4.819522529128982 35.961052717347016 21.294711969363643 37.924712784160356 6 9.329422309133871 34.909964963741544 11.15456693554958 44.606045791575 7 8.339444308644993 34.8203373258372 11.720850647763383 45.11936771775442 8 7.985007740568728 46.72451723295038 12.710828648252262 32.57964637822863 9 11.602705125071294 31.671148048561882 11.733072598386702 44.99307422798012 10-11 10.356066161492707 31.701702925120184 22.79597490426139 35.14625600912573 12-13 10.05662837122138 28.179744153833617 22.889676525706836 38.873950949238164 14-15 13.242483500366658 30.8644993074228 19.518455145441212 36.374562046769334 16-17 17.79312311578261 30.813574513158965 19.09679784893669 32.29650452212173 18-19 14.256905402102177 28.911024199462233 18.30440805019148 38.527662348244114 20-21 12.657866862217876 29.458974985741058 19.62845270105109 38.25470545098998 22-23 19.595860832722238 29.14731524484641 16.929438605068036 34.327385317363316 24-25 15.758168336999919 34.48627067546647 16.214454493603846 33.54110649392976 26-27 12.580461174936852 35.35810315326326 20.69583638882099 31.365599282978895 28-29 16.057606127271246 30.13729324533529 24.07927971970993 29.72582090768353 30-31 15.829463048969284 29.32860751242565 25.67628126782368 29.165648170781388 32-33 12.58453515847796 35.659577935305144 22.73486515114479 29.021021755072105 34-35 13.08563513403406 32.97685977348652 22.386539558380182 31.55096553409924 36-37 13.800619245498247 29.324533528884544 24.380754501751813 32.4940927238654 38-39 13.894320866943696 25.354436568076267 28.208262038621363 32.542980526358676 40-41 11.828811211602705 26.798663733398513 26.356636519188463 35.01588853581031 42-43 8.555365436323639 25.5622097286727 29.491566854069912 36.39085798093375 44-45 10.12588609142019 28.06363562291208 29.615823352073654 32.194654933594066 46-47 10.233846655259512 30.34914038947283 26.727369021429155 32.68964393383851 48-49 10.217550721095087 32.78538254705451 21.954697303022897 35.042369428827506 50-51 9.973111708628696 31.000977756049863 21.288600994051983 37.737309541269454 52-53 9.484233683695917 30.080257475759797 23.30725983867025 37.12824900187403 54-55 9.708302778456774 30.03748064857818 21.677666422227652 38.57655015073739 56-57 12.900268882913712 30.21266194084576 19.740487248431513 37.14658192780902 58-59 12.472500611097532 29.27157174285016 20.494174203536218 37.761753442516095 60-61 10.07496129715636 27.254949890002443 21.64303756212825 41.02705125071295 62-63 11.651592927564574 29.442679051576633 19.738450256660965 39.167277764197834 64-65 12.54583231483745 30.459137945082702 17.08221298785953 39.91281675222032 66-67 10.37032510388658 36.1667888861729 15.367065917053695 38.095820092886825 68-69 10.494581601890328 37.03658437219914 14.859854966185937 37.608979059724604 70-71 9.435345881202641 38.81284119612157 15.65835574024281 36.09345718243298 72-73 9.777560498655586 39.146907846492304 16.137048806322824 34.93848284852929 74-75 12.568239224313535 38.06730220809908 15.955756538743584 33.4087020288438 76 14.478937505092478 35.993644585675874 15.896683777397538 33.6307341318341 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 1.5 10 1.5 11 1.5 12 3.0 13 4.5 14 7.5 15 8.0 16 6.0 17 5.0 18 9.0 19 16.0 20 21.5 21 24.0 22 25.5 23 32.0 24 44.5 25 52.0 26 64.0 27 89.0 28 130.5 29 159.0 30 192.5 31 225.5 32 280.0 33 335.0 34 379.5 35 485.0 36 610.5 37 675.0 38 733.0 39 903.0 40 1083.5 41 1246.5 42 1341.0 43 1379.5 44 1489.5 45 1580.0 46 1599.0 47 1617.0 48 1586.0 49 1555.5 50 1574.0 51 1443.5 52 1238.0 53 1108.0 54 1053.0 55 977.0 56 827.0 57 680.0 58 607.0 59 537.5 60 430.0 61 356.5 62 321.0 63 277.5 64 213.0 65 158.5 66 109.5 67 94.0 68 88.5 69 70.5 70 43.5 71 29.0 72 28.0 73 25.0 74 20.5 75 18.0 76 13.5 77 9.5 78 10.5 79 11.0 80 8.0 81 6.5 82 7.5 83 7.0 84 6.0 85 6.0 86 5.0 87 3.0 88 2.5 89 1.5 90 3.0 91 3.0 92 1.0 93 2.5 94 2.5 95 1.5 96 2.0 97 4.0 98 5.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6110975311659741 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 24546.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.25348325592765 #Duplication Level Percentage of deduplicated Percentage of total 1 95.3385757972914 88.90654281756701 2 3.1236347750109217 5.825796463782287 3 1.0484927916120577 2.9332681495966755 4 0.29270423765836606 1.0918275890165403 5 0.109217999126256 0.5092479426383117 6 0.04805591961555264 0.2688829137130286 7 0.021843599825251202 0.14258942393872728 8 0.0 0.0 9 0.0 0.0 >10 0.01747487986020096 0.321844699747413 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTGGTC 31 0.1262934897743013 Illumina Single End PCR Primer 1 (95% over 22bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.012221950623319482 0.0 6 0.0 0.0 0.0 0.012221950623319482 0.0 7 0.0 0.0 0.0 0.012221950623319482 0.0 8 0.0 0.0 0.0 0.012221950623319482 0.0 9 0.0 0.0 0.0 0.012221950623319482 0.0 10 0.0 0.0 0.0 0.024443901246638963 0.0 11 0.0 0.0 0.0 0.024443901246638963 0.0 12 0.0 0.0 0.0 0.028517884787745456 0.0 13 0.0 0.0 0.0 0.03259186832885195 0.0 14 0.0 0.0 0.0 0.03259186832885195 0.0 15 0.0 0.0 0.0 0.03259186832885195 0.0 16 0.0 0.0 0.0 0.036665851869958445 0.0 17 0.0 0.0 0.0 0.036665851869958445 0.0 18 0.0 0.0 0.0 0.04073983541106494 0.0 19 0.0 0.0 0.0 0.048887802493277926 0.0 20 0.0 0.0 0.0 0.048887802493277926 0.0 21 0.0 0.0 0.0 0.048887802493277926 0.0 22 0.0 0.0 0.0 0.048887802493277926 0.0 23 0.0 0.0 0.0 0.048887802493277926 0.0 24 0.0 0.0 0.0 0.048887802493277926 0.0 25 0.0 0.0 0.0 0.048887802493277926 0.0 26 0.0 0.0 0.0 0.048887802493277926 0.0 27 0.0 0.0 0.0 0.07740568728102339 0.0 28 0.0 0.0 0.0 0.08147967082212988 0.0 29 0.0 0.0 0.0 0.08555365436323638 0.0 30 0.0 0.0 0.0 0.10592357206876885 0.0 31 0.0 0.0 0.0 0.11814552269208832 0.0 32 0.0 0.0 0.0 0.15073739102094028 0.0 33 0.0 0.0 0.0 0.17110730872647276 0.0 34 0.0 0.0 0.0 0.2077731605964312 0.0 35 0.0 0.0 0.0 0.2648089301719221 0.0 36 0.0 0.0 0.0 0.36258453515847794 0.0 37 0.0 0.0 0.0 0.4766560743094598 0.0 38 0.0 0.0 0.0 0.6355414324126131 0.0 39 0.0 0.0 0.0 0.8025747575979792 0.0 40 0.0 0.0 0.0 0.9573861321600261 0.0 41 0.0 0.0 0.0 1.1244194573453923 0.0 42 0.0 0.0 0.0 1.385154403976208 0.0 43 0.0 0.0 0.0 1.625519432901491 0.0 44 0.0 0.0 0.0 1.8740324289089871 0.0 45 0.0 0.0 0.0 2.130693391998696 0.0 46 0.0 0.0 0.0 2.3221706184307016 0.0 47 0.0 0.0 0.0 2.5136478448627066 0.0 48 0.0 0.0 0.0 2.6929031206713927 0.0 49 0.0 0.0 0.0 2.929194166055569 0.0 50 0.0 0.0 0.0 3.116597408946468 0.0 51 0.0 0.0 0.0 3.2795567505907277 0.0 52 0.0 0.0 0.0 3.5565876313859692 0.0 53 0.0 0.0 0.0 3.7154729894891223 0.0 54 0.0 0.0 0.0 3.882506314674489 0.0 55 0.0 0.0 0.0 4.029169722154323 0.0 56 0.0 0.0 0.0 4.220646948586328 0.0 57 0.0 0.0 0.0 4.432494092723865 0.0 58 0.0 0.0 0.0 4.6484152204025095 0.0 59 0.0 0.0 0.0 4.8195225291289825 0.0 60 0.0 0.0 0.0 4.994703821396562 0.0 61 0.0 0.0 0.0 5.206550965534099 0.0 62 0.0 0.0 0.0 5.3206225046850815 0.0 63 0.0 0.0 0.0 5.495803796952661 0.0 64 0.0 0.0 0.0 5.573209484233684 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACAGAG 15 0.0021999525 69.99184 19 GAAACAC 45 2.401248E-8 54.88615 1 GAAACCC 65 1.5643309E-10 48.854702 1 GAAAACA 50 1.5847055E-4 35.283947 1 TCGGTGG 45 0.0042298683 31.107483 42 CGGTGGT 45 0.0042298683 31.107483 43 TCTCGGT 50 0.0070796493 27.996735 40 CTCGGTG 50 0.0070796493 27.996735 41 CGACGAA 80 0.002595844 21.872448 19 AAAACCC 90 0.004898207 19.602196 1 AACCCAA 90 0.00513722 19.442177 3 AAACCCA 130 1.0058764E-4 18.843956 2 AAACCCC 150 1.4029034E-5 18.66449 2 GCTGCCG 150 1.4029034E-5 18.66449 14 CCGACGA 160 2.4454475E-5 17.49796 18 CGCTGCC 170 4.11522E-5 16.468668 13 ACGCTGC 170 4.11522E-5 16.468668 12 CTGCCGA 150 3.0068235E-4 16.331429 15 CTGACGC 175 5.2751337E-5 15.998133 9 TGACGCT 185 8.4823165E-5 15.13337 10 >>END_MODULE