FastQCFastQC Report
Wed 25 May 2016
SRR1780414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24546
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT415016.90703169559195No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT398816.2470463619327RNA PCR Primer, Index 35 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC14355.846166381487819No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC7072.8803063635622914No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG4091.6662592683125559No Hit
TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT3521.434042206469486No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT2170.8840544284201092No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1940.7903528069746599No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1900.7740568728102338No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1600.6518373665770391No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1410.5744316792960156No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1400.5703576957549091No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT1290.5255438768027377No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT1250.5092479426383117No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC1150.46850810722724684No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT1090.4440642059806078No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT1060.4318422553572883No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC920.3748064857817974No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT870.35443656807626495No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG850.346288600994052No Hit
TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC800.32591868328851953No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG750.30554876558298705No Hit
CTTATACACATCTCCGAGCCCACGCGACTGGGTTTCATCTCGTATGCCGT590.24036502892528316No Hit
ATACACATCTCCGAGCCCACGAGACTGGGCTTCATCTCGTATGCCGTCTT480.1955512099731117TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGCCTGGGTTTCATCTCGTATGCCGT440.17925527580868575No Hit
CTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT430.17518129226757925No Hit
TCTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT420.17110730872647273No Hit
ACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCT380.15481137456204677RNA PCR Primer, Index 35 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC370.15073739102094028No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTC360.14666340747983378No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC350.14258942393872728No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT340.1385154403976208No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTAGTATGCCGT320.13036747331540782No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCCTCTCGTATGCCGTCTT310.1262934897743013No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCG310.1262934897743013No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTCTGCCGTCTT310.1262934897743013No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTC280.11407153915098184No Hit
CTTATACACATATCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT280.11407153915098184No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC270.10999755560987534No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG260.10592357206876885No Hit
ATACACATCTCCGAGCCCACGAGACTGGGATTCATCTCGTATGCCGTCTT260.10592357206876885RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCCT260.10592357206876885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACACC252.758834E-670.0000170
GGGTTTA150.00219893570.030
ATCTAGT150.00219893570.037
CATCTAG150.00219893570.036
TTATCAC150.00219893570.02
TGTTTAA150.00219893570.05
GGTTTAA150.00219893570.031
GTTTAAT150.00219893570.032
TAATCTC150.00219893570.035
CTAGTAT150.00219893570.039
CTTAATA353.5161065E-970.01
TAATACA353.5161065E-970.03
TCATCTA150.00219893570.035
TTAATAC353.5161065E-970.02
TTTAATC150.00219893570.033
CTTTACA2150.068.372091
TTTACAC2150.066.744192
CTATACA650.064.615391
TTACACA2300.062.3913043
AATACAC401.0104486E-861.254
Produced by FastQC (version 0.11.5)