##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780414_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24546 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89122463945246 34.0 34.0 34.0 31.0 34.0 2 33.28269371791738 34.0 34.0 34.0 32.0 34.0 3 33.48402998451886 34.0 34.0 34.0 34.0 34.0 4 33.520084738857655 34.0 34.0 34.0 34.0 34.0 5 33.56013199706673 34.0 34.0 34.0 34.0 34.0 6 37.341481300415545 38.0 38.0 38.0 37.0 38.0 7 36.918764768190336 38.0 37.0 38.0 35.0 38.0 8 36.95807870936201 38.0 37.0 38.0 35.0 38.0 9 36.70202884380347 38.0 37.0 38.0 34.0 38.0 10-11 36.42454982481871 38.0 37.0 38.0 34.0 38.0 12-13 36.12582498166707 38.0 36.5 38.0 33.5 38.0 14-15 36.166218528477145 38.0 37.0 38.0 33.5 38.0 16-17 36.15699095575654 38.0 36.5 38.0 32.5 38.0 18-19 36.263199706673184 38.0 37.0 38.0 33.5 38.0 20-21 36.11877699014096 38.0 37.0 38.0 33.0 38.0 22-23 36.0728835655504 38.0 36.5 38.0 32.5 38.0 24-25 34.82989081724109 37.5 34.5 38.0 28.0 38.0 26-27 35.023567994785296 37.0 35.0 38.0 30.0 38.0 28-29 35.030412287134354 37.5 35.5 38.0 29.0 38.0 30-31 34.462111953067705 37.0 34.0 38.0 26.5 38.0 32-33 35.04892854232869 37.0 35.5 38.0 27.0 38.0 34-35 34.09180721909884 37.0 34.0 38.0 24.5 38.0 36-37 34.11244194573454 37.0 34.0 38.0 25.0 38.0 38-39 32.429438605068036 36.5 31.5 38.0 11.5 38.0 40-41 33.6038254705451 37.0 34.0 38.0 23.5 38.0 42-43 34.984254053613626 37.0 34.5 38.0 29.0 38.0 44-45 35.347449686303264 37.0 36.0 38.0 30.5 38.0 46-47 35.33752953638067 38.0 36.0 38.0 31.0 38.0 48-49 34.90729650452212 37.5 35.0 38.0 28.0 38.0 50-51 34.89749857410576 37.0 34.5 38.0 29.0 38.0 52-53 34.66316304082132 37.0 34.0 38.0 27.0 38.0 54-55 35.009023873543555 37.0 35.5 38.0 27.0 38.0 56-57 34.89662266764442 37.0 35.5 38.0 27.0 38.0 58-59 34.704208424997965 37.0 35.0 38.0 27.0 38.0 60-61 34.62619164018577 37.0 34.5 38.0 26.0 38.0 62-63 35.27719384013689 37.0 36.0 38.0 31.0 38.0 64-65 35.24443901246639 38.0 36.0 38.0 29.0 38.0 66-67 33.47097286726962 37.0 33.5 38.0 22.5 38.0 68-69 29.86056791330563 34.5 24.0 38.0 11.0 38.0 70-71 26.591949808522774 28.5 16.0 37.0 11.0 38.0 72-73 23.690601319970668 23.5 11.0 34.5 11.0 38.0 74-75 23.2805548765583 23.0 11.0 34.0 11.0 38.0 76 23.422920231402266 24.0 11.0 34.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5304600673877786 1101 2 0.6297649086892463 1101 3 0.2713198553068139 1101 4 0.36142810090866817 1101 5 0.0024847324262040615 1101 6 -0.8064834633838913 1101 7 0.1603788181667838 1101 8 0.2557570239129774 1101 9 -0.1237746500197332 1101 10-11 -0.368916554511145 1101 12-13 0.01689683149101029 1101 14-15 0.2739461206614209 1101 16-17 -0.3963953747127249 1101 18-19 -1.2822411519333343 1101 20-21 0.0014685034197370328 1101 22-23 0.9909720213163311 1101 24-25 -1.470462928965489 1101 26-27 -0.48995836706431106 1101 28-29 1.0163321003291585 1101 30-31 0.8265866591237625 1101 32-33 0.7843386661575025 1101 34-35 0.02122682436223755 1101 36-37 0.592784823027614 1101 38-39 -0.10198857762333091 1101 40-41 -0.0371627006075812 1101 42-43 -0.06448823203022158 1101 44-45 -0.9080971008943521 1101 46-47 0.4402956953361894 1101 48-49 -3.0665883107118503 1101 50-51 -3.148906651339548 1101 52-53 -4.186598208315953 1101 54-55 -2.3806682191370356 1101 56-57 -2.487348405018686 1101 58-59 -3.0276647591407126 1101 60-61 -3.3103044850168217 1101 62-63 -2.077298314694957 1101 64-65 -1.8097141523666949 1101 66-67 -3.6530822875475835 1101 68-69 -1.3206131055364736 1101 70-71 -0.8675507888063123 1101 72-73 -1.2080037659608571 1101 74-75 -0.6108169568649942 1101 76 -0.7956880865596005 1102 1 -0.05777522672986635 1102 2 0.3797649086892463 1102 3 0.3889669141303429 1102 4 0.4349575126733711 1102 5 0.414249438308552 1102 6 0.29645771308669566 1102 7 0.10155528875502284 1102 8 0.6675217297953324 1102 9 0.8468135852743899 1102 10-11 0.726671680782971 1102 12-13 1.1418968314910174 1102 14-15 0.22247553242613094 1102 16-17 0.30213403705197805 1102 18-19 -0.5469470342862763 1102 20-21 0.214703797537382 1102 22-23 -0.1340279786836689 1102 24-25 -6.088109987789018 1102 26-27 -1.004664249417253 1102 28-29 0.6707438650350426 1102 30-31 2.2089396003002335 1102 32-33 0.8063974896869226 1102 34-35 0.18299153024458548 1102 36-37 -1.988097529913567 1102 38-39 0.1553643635531401 1102 40-41 1.0584255346865348 1102 42-43 1.1487470620874305 1102 44-45 1.4669028991056408 1102 46-47 1.8520604012185373 1102 48-49 -4.882764781300086 1102 50-51 -1.5312595925160224 1102 52-53 0.3648723799193405 1102 54-55 1.1708023690982543 1102 56-57 1.247945712628372 1102 58-59 -0.3511941709054227 1102 60-61 -6.229422132075648 1102 62-63 -2.194945373518486 1102 64-65 0.23440349469213118 1102 66-67 1.5969177124524165 1102 68-69 -0.35737781141882863 1102 70-71 -1.3822566711592543 1102 72-73 -3.1050625894902666 1102 74-75 -1.1990522509826391 1102 76 -1.0015704395007745 1103 1 0.5745777144466047 1103 2 0.720451183199053 1103 3 0.3089015546532252 1103 4 0.40717973489559256 1103 5 0.19529518994254147 1103 6 0.5521766673350612 1103 7 0.649757903134109 1103 8 0.7271622526711496 1103 9 0.20465672252928613 1103 10-11 0.3741390010444121 1103 12-13 -0.9696227763521179 1103 14-15 -0.3208251211686388 1103 16-17 0.6317908997970747 1103 18-19 0.9546869526418362 1103 20-21 -1.0839073135737323 1103 22-23 0.16131515857123446 1103 24-25 -3.101590379945879 1103 26-27 -0.3424910468028699 1103 28-29 0.41216543366249425 1103 30-31 1.3964396003002335 1103 32-33 1.8725739602751545 1103 34-35 1.0874032949504695 1103 36-37 -0.007705373050818309 1103 38-39 1.2386976968864687 1103 40-41 0.24960200527476673 1103 42-43 0.4935183039174902 1103 44-45 1.8925564938768815 1103 46-47 1.7319623620028608 1103 48-49 -1.0388105329340718 1103 50-51 0.5823024989872465 1103 52-53 1.6152808766513687 1103 54-55 0.7112435455688484 1103 56-57 1.1576679348505934 1103 58-59 -0.7159817526047689 1103 60-61 -4.400173766062572 1103 62-63 -0.8068734780936495 1103 64-65 1.5436355208359203 1103 66-67 0.9363784967661459 1103 68-69 1.2876385284504508 1103 70-71 0.9420897340695014 1103 72-73 -3.608330563346474 1103 74-75 -0.6794444078453878 1103 76 -1.870851485252409 1104 1 -0.21489596976392278 1104 2 0.3015915340762447 1104 3 0.053053601436850784 1104 4 0.15864172319968617 1104 5 -0.5795586112270499 1104 6 0.09676731060991273 1104 7 0.363939189683812 1104 8 0.2936827205074053 1104 9 0.8932532137573617 1104 10-11 -1.5399691860900901 1104 12-13 0.3845438903145464 1104 14-15 0.23253745193077435 1104 16-17 -0.435095065115199 1104 18-19 0.6469384146301351 1104 20-21 -0.18003304456787816 1104 22-23 0.458464281378248 1104 24-25 0.024506111282192933 1104 26-27 0.888137608167888 1104 28-29 2.2137005213817886 1104 30-31 0.2155185476686583 1104 32-33 0.17520553922252446 1104 34-35 -1.2941756524179553 1104 36-37 0.30369813572110615 1104 38-39 1.6882591003952427 1104 40-41 1.6311809526431915 1104 42-43 0.6309452045022894 1104 44-45 0.16814128919851612 1104 46-47 0.5916114848098744 1104 48-49 0.6110432682355196 1104 50-51 1.4865422650691187 1104 52-53 0.8524884789905514 1104 54-55 0.510585650832013 1104 56-57 -0.4165279715821484 1104 58-59 -1.3186864309673396 1104 60-61 0.506258982475444 1104 62-63 -0.11754599271353783 1104 64-65 0.6492641758066782 1104 66-67 -1.3848934330584228 1104 68-69 -0.02997843061387684 1104 70-71 -0.8006003244100306 1104 72-73 -0.6704650662704523 1104 74-75 -0.5829531797752097 1104 76 0.2329496843382337 1105 1 0.7145777144466052 1105 2 0.5621178498657144 1105 3 0.12779044354211067 1105 4 0.2482908460067037 1105 5 0.12640630105365602 1105 6 -0.23060111044271991 1105 7 0.5025356809118833 1105 8 -0.782282191773298 1105 9 0.6613233891959567 1105 10-11 0.6660834454888516 1105 12-13 -0.24878944301878647 1105 14-15 0.42473043438691604 1105 16-17 0.3542908997970784 1105 18-19 1.0124647304196088 1105 20-21 0.44637046420405113 1105 22-23 0.05881515857122821 1105 24-25 0.3975762867207919 1105 26-27 -0.29387993569175563 1105 28-29 0.5413321003291571 1105 30-31 1.2256062669668992 1105 32-33 1.3309072936084831 1105 34-35 -1.6075967050495308 1105 36-37 1.0089612936158474 1105 38-39 -0.2829689697801925 1105 40-41 0.07460200527476957 1105 42-43 0.5979627483619367 1105 44-45 1.3686676049879907 1105 46-47 1.5386290286695257 1105 48-49 -0.6349216440451855 1105 50-51 0.25952472120947334 1105 52-53 0.6872253210958092 1105 54-55 0.2837435455688464 1105 56-57 0.32461237929503994 1105 58-59 1.367629358506342 1105 60-61 1.0037151228263141 1105 62-63 -0.6896512558714321 1105 64-65 1.087246631947032 1105 66-67 1.7355451634328105 1105 68-69 -0.1254170271051045 1105 70-71 0.10431195629172407 1105 72-73 -1.288886118902031 1105 74-75 -0.8894444078453851 1105 76 0.23470407030314533 1106 1 -0.7342458149651634 1106 2 0.3062354969245433 1106 3 0.4036727964832849 1106 4 0.22907515973219716 1106 5 0.193661203014436 1106 6 0.38469300720434063 1106 7 -1.0749152994802742 1106 8 -0.06777238785173267 1106 9 -1.1678922970785521 1106 10-11 -0.0821518486287971 1106 12-13 1.3036615373733724 1106 14-15 -0.858406820515043 1106 16-17 -0.12433655118331188 1106 18-19 0.3868764951254917 1106 20-21 0.14852732694915005 1106 22-23 0.108619080139853 1106 24-25 -0.36752175249490193 1106 26-27 0.7453357505827469 1106 28-29 0.6339791591526875 1106 30-31 0.4736454826531755 1106 32-33 0.25492690145162555 1106 34-35 -8.319991671825733E-4 1106 36-37 1.394255411262904 1106 38-39 -0.06522387174097588 1106 40-41 1.6907784758629987 1106 42-43 0.7958058856168364 1106 44-45 0.6948440755762277 1106 46-47 0.35941334239501543 1106 48-49 1.8525293363469757 1106 50-51 -0.7592007689866094 1106 52-53 -0.1571864436100725 1106 54-55 -0.11596233678409362 1106 56-57 -0.22999546384222214 1106 58-59 0.4208646526239974 1106 60-61 0.7558719855714173 1106 62-63 0.3932899205991589 1106 64-65 -1.787655328837289 1106 66-67 0.1925059477465325 1106 68-69 0.8337986591694033 1106 70-71 -1.5807860829239644 1106 72-73 -0.09770964831379558 1106 74-75 -1.7505228392179362 1106 76 -1.6780410277360716 1107 1 0.8087549296364784 1107 2 -0.6049707577292196 1107 3 0.11867651949147984 1107 4 0.19445118355944402 1107 5 -0.2990789310138595 1107 6 0.34560142120285064 1107 7 0.6274301956798212 1107 8 -0.24945518755389173 1107 9 -0.5474529821120626 1107 10-11 0.8162100277673403 1107 12-13 0.09340464980822105 1107 14-15 0.2484435145556887 1107 16-17 0.8039955411472945 1107 18-19 0.523603970925933 1107 20-21 0.9409696203221927 1107 22-23 0.3118953273476066 1107 24-25 0.7910783964254264 1107 26-27 -0.1504200200799417 1107 28-29 0.7046232395696634 1107 30-31 0.8703320053635295 1107 32-33 1.940611934958703 1107 34-35 1.1301248139378046 1107 36-37 2.079847369565215 1107 38-39 1.9480858825404752 1107 40-41 0.4315640305912183 1107 42-43 1.1489332124969636 1107 44-45 1.305882794861411 1107 46-47 1.4070678472349272 1107 48-49 1.2229686513134652 1107 50-51 1.500199826694704 1107 52-53 1.582668359070496 1107 54-55 0.7262751911384697 1107 56-57 1.0106039404764715 1107 58-59 1.0810470800253356 1107 60-61 1.4375547852735764 1107 62-63 0.4359183643817417 1107 64-65 0.7436179399639116 1107 66-67 -1.7595603217992632 1107 68-69 2.039308711291522 1107 70-71 0.4955355849837062 1107 72-73 1.1225062861612578 1107 74-75 0.3532560140955425 1107 76 0.1520036483622178 1108 1 0.2879110477799358 1108 2 0.042117849865718426 1108 3 -0.0988762231245559 1108 4 0.394957512673372 1108 5 0.24640630105365346 1108 6 -0.27060111044271906 1108 7 -0.12413098575478188 1108 8 -0.5156155251066323 1108 9 0.31465672252928556 1108 10-11 0.39941677882218585 1108 12-13 1.1445438903145515 1108 14-15 0.5247304343869104 1108 16-17 -0.8523757668695922 1108 18-19 0.05913139708627568 1108 20-21 0.9197037975373803 1108 22-23 -0.24118484142876895 1108 24-25 0.5975762867207877 1108 26-27 -1.2338799356917605 1108 28-29 -0.8186678996708352 1108 30-31 0.8122729336335723 1108 32-33 0.7642406269418203 1108 34-35 1.2990699616171355 1108 36-37 1.7089612936158431 1108 38-39 1.597031030219803 1108 40-41 0.7479353386081016 1108 42-43 -0.7153705849713887 1108 44-45 0.15533427165466662 1108 46-47 0.9119623620028534 1108 48-49 0.4050783559548137 1108 50-51 0.5995247212094696 1108 52-53 -0.17277467890419018 1108 54-55 -0.44292312109782017 1108 56-57 0.25127904596170225 1108 58-59 -0.04570397482698496 1108 60-61 1.9703817894929827 1108 62-63 1.0236820774619062 1108 64-65 0.8739132986137008 1108 66-67 0.7488784967661459 1108 68-69 2.3479163062282282 1108 70-71 -1.9290213770416074 1108 72-73 0.2044472144313012 1108 74-75 0.4172222588212833 1108 76 -0.04529592969685581 1109 1 -1.6049094650405742 1109 2 0.08263067037853489 1109 3 0.15291864867031535 1109 4 0.02470110241696233 1109 5 -0.12949113484378216 1109 6 -0.38906264890425746 1109 7 -0.37695149857529486 1109 8 0.311563962072853 1109 9 0.22388749176005973 1109 10-11 0.018903958309365976 1109 12-13 -0.10263559686494261 1109 14-15 0.2860124856689623 1109 16-17 0.7199319254381038 1109 18-19 0.3093878073426879 1109 20-21 -0.006450048616464699 1109 22-23 -0.02297971322364134 1109 24-25 -1.1993467902022843 1109 26-27 0.763812372000551 1109 28-29 -1.2304627714657173 1109 30-31 0.715349856710489 1109 32-33 1.1706508833520743 1109 34-35 -0.1209300383828662 1109 36-37 1.4730638577184152 1109 38-39 -0.5914305082417322 1109 40-41 0.8746020052747667 1109 42-43 1.1046294150286045 1109 44-45 0.8402060665264557 1109 46-47 1.1422187722592696 1109 48-49 0.725078355954814 1109 50-51 0.11542215710690584 1109 52-53 -0.9899541660836775 1109 54-55 -0.7006154287901225 1109 56-57 0.16461237929504335 1109 58-59 -0.35006294918596126 1109 60-61 0.5175612766724669 1109 62-63 0.4121436159234477 1109 64-65 1.8839132986136917 1109 66-67 -0.4626599647723211 1109 68-69 -0.9041349758230552 1109 70-71 0.1514914434712118 1109 72-73 0.4675241375082244 1109 74-75 0.19235046394948796 1109 76 0.6782938138928891 1110 1 -0.2916194686519873 1110 2 -0.6181638402751304 1110 3 0.06750875340126328 1110 4 0.04059131549027484 1110 5 0.23363634800200828 1110 6 0.14235663603615478 1110 7 0.5184981222264398 1110 8 0.737530014799475 1110 9 1.1189759713555816 1110 10-11 -0.09250810380692087 1110 12-13 0.9267974114413136 1110 14-15 1.127922922649823 1110 16-17 1.0792674256186743 1110 18-19 0.5071126177435517 1110 20-21 1.1002671778190773 1110 22-23 -0.34954164894050876 1110 24-25 -0.12477113111958715 1110 26-27 1.9831153694725572 1110 28-29 0.7610504101883109 1110 30-31 0.4589396003002335 1110 32-33 1.2651795940779706 1110 34-35 1.7616990695983574 1110 36-37 1.908961293615846 1110 38-39 1.079660138201028 1110 40-41 -0.9493416566970652 1110 42-43 0.3088547671412769 1110 44-45 1.4666957740020763 1110 46-47 1.537126681251685 1110 48-49 2.551369435767022 1110 50-51 0.5691960827118123 1110 52-53 1.085253490109892 1110 54-55 0.3617717145829289 1110 56-57 1.490903459107244 1110 58-59 -0.8980044443105513 1110 60-61 0.21817521672302576 1110 62-63 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2116 62-63 0.8605241827250651 2116 64-65 -2.6029288066494587 2116 66-67 -0.6019986962163202 2116 68-69 -2.2931363253507193 2116 70-71 1.6555400264671647 2116 72-73 1.6146226530277907 2116 74-75 2.6144152412774204 2116 76 1.276809333461042 2117 1 -0.02157613170724204 2117 2 0.4736563114041772 2117 3 -0.10990186415019565 2117 4 -0.16119633348047557 2117 5 -0.10385010920276017 2117 6 0.14939888955728264 2117 7 0.48843311680931834 2117 8 -0.23971808920919813 2117 9 -1.3081637902912249 2117 10-11 -0.3112242468188384 2117 12-13 -1.3846868789162272 2117 14-15 0.04883299848947331 2117 16-17 -3.0813501258439473 2117 18-19 -2.729073731118852 2117 20-21 -0.8718346640010779 2117 22-23 -0.45246689271082374 2117 24-25 -3.8980647389202403 2117 26-27 -3.6528542946661204 2117 28-29 -4.118283284286225 2117 30-31 -0.8775988612382264 2117 32-33 -3.069733732032539 2117 34-35 0.2027879103350827 2117 36-37 -1.917961783307227 2117 38-39 -1.09463563644686 2117 40-41 0.3457558514286134 2117 42-43 -1.337678277279089 2117 44-45 -0.9963323950120042 2117 46-47 -1.4539350738945842 2117 48-49 -2.2588960030195437 2117 50-51 -0.6217573300725832 2117 52-53 0.09337916724965112 2117 54-55 -1.4570256852003851 2117 56-57 -0.5309004412177813 2117 58-59 -2.311601410724421 2117 60-61 -1.3894900053788177 2117 62-63 -1.2865743327945083 2117 64-65 -2.85326618856579 2117 66-67 -1.7543266314389854 2117 68-69 -0.46823753992561734 2117 70-71 -1.851713684733916 2117 72-73 -0.8465784265943377 2117 74-75 0.942350463949488 2117 76 1.2359861215851957 2118 1 -0.435226207122021 2118 2 0.46800020280689125 2118 3 -0.9247585760657344 2118 4 0.3369182969870934 2118 5 0.08091610497522339 2118 6 -0.23295405161918836 2118 7 0.6358690142452161 2118 8 0.5204629062659194 2118 9 -3.6139707284511005 2118 10-11 -1.1311714564719324 2118 12-13 -1.6742796390972217 2118 14-15 0.8670833755633822 2118 16-17 -3.3253169433401766 2118 18-19 -0.983221544090199 2118 20-21 -1.189707967168502 2118 22-23 0.3046975115124084 2118 24-25 0.36777236515216316 2118 26-27 -4.6370171905937205 2118 28-29 -1.0449424094747641 2118 30-31 -0.7469427526409405 2118 32-33 -1.1200730985483744 2118 34-35 -0.39543984230443385 2118 36-37 -4.6498622357959185 2118 38-39 0.442129069435488 2118 40-41 -1.7626528966860207 2118 42-43 0.36933529738154647 2118 44-45 -3.204665728345333 2118 46-47 0.16333491102246 2118 48-49 0.24468619909207234 2118 50-51 -1.791063514084648 2118 52-53 -2.291990365178698 2118 54-55 -1.1821388073723256 2118 56-57 0.2724555165499396 2118 58-59 -1.694331425807377 2118 60-61 -2.20491232815408 2118 62-63 -3.4547492950871117 2118 64-65 -0.09157689746473352 2118 66-67 -1.015827385586796 2118 68-69 -3.048554282007064 2118 70-71 -0.43372725939455137 2118 72-73 -0.4555527855686954 2118 74-75 0.6293791215663802 2118 76 -2.5652959296968554 2119 1 0.4658820622726907 2119 2 -2.1074473675255874 2119 3 -0.07394868689267042 2119 4 -0.3911294438483708 2119 5 -0.3530139888014219 2119 6 -0.7853837191383661 2119 7 -0.3568846089431901 2119 8 0.33887722851655866 2119 9 0.07059875151479389 2119 10-11 -0.004786119728535709 2119 12-13 -0.32197784881589087 2119 14-15 -0.6275884061927997 2119 16-17 -0.3129554770145191 2119 18-19 -0.2023178782760482 2119 20-21 -1.6385570720278366 2119 22-23 -1.350460203747609 2119 24-25 0.09859077947440653 2119 26-27 0.23510557155461953 2119 28-29 -0.8695374648882321 2119 30-31 0.15459177421328008 2119 32-33 -0.5567738658117989 2119 34-35 -0.004988009397358439 2119 36-37 -2.2540821846450214 2119 38-39 -1.5239834625338204 2119 40-41 -1.1906153860295774 2119 42-43 0.18071637155034637 2119 44-45 0.1286676049879958 2119 46-47 -0.04339995683771747 2119 48-49 -1.919849180277069 2119 50-51 0.08894501106453845 2119 52-53 -0.44842685281723504 2119 54-55 -0.9219086283441982 2119 56-57 -2.7303151569368467 2119 58-59 -0.14019672845017794 2119 60-61 -0.6399080655794762 2119 62-63 -0.5900860384801234 2119 64-65 -0.6015939477631136 2119 66-67 0.9300379170559978 2119 68-69 -0.5677358676848137 2119 70-71 0.23315253600186736 2119 72-73 2.0915486637066643 2119 74-75 0.8278019689662095 2119 76 1.5361533456654648 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 3.0 22 3.0 23 15.0 24 37.0 25 105.0 26 176.0 27 310.0 28 570.0 29 837.0 30 1204.0 31 1798.0 32 2475.0 33 3444.0 34 4530.0 35 4211.0 36 2154.0 37 2671.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.938645807870936 28.285667725902385 10.641245009370163 50.134441456856514 2 5.068035525136478 8.33129634156278 74.22798011896032 12.372688014340422 3 5.255438768027377 33.73258372036177 52.395502322170614 8.616475189440234 4 3.821396561557891 35.78587142507944 29.703413998207445 30.68931801515522 5 3.6828811211602703 50.38295445286401 39.383198891876475 6.550965534099243 6 6.925772019881039 42.32054102501426 10.94679377495315 39.806893180151555 7 7.162063065265216 49.76778293815693 10.091257231320785 32.97889676525707 8 6.074309459789783 40.617615904831744 28.493440886498817 24.814633748879654 9 5.9806078383443335 24.00798500774057 8.70202884380347 61.309378310111626 10-11 6.569298460034221 24.307422798011896 38.82098916320378 30.302289578750102 12-13 17.6281267823678 8.528884543306445 31.31263749694451 42.53035117738124 14-15 24.445938238409518 19.106982807789457 11.604742116841848 44.84233683695918 16-17 28.8865802982156 26.896439338385076 7.306689480974497 36.91029088242483 18-19 23.508922023955023 23.240039110241995 7.437056954289904 45.81398191151308 20-21 19.40438360629023 15.841684999592601 7.125397213395258 57.628534180721914 22-23 25.285178847877454 35.12588609142019 7.1926179418235145 32.39631711887884 24-25 29.28175670170293 40.37521388413591 7.559276460523099 22.783752953638068 26-27 34.82441130937831 30.978570846573778 19.512344170129552 14.684673673918358 28-29 38.97987452130693 12.562128249001875 26.863847470056225 21.59414975963497 30-31 48.109671636926585 7.714087835085147 35.3214373013933 8.854803226594965 32-33 28.281593742361284 7.8729731931883 46.31711887883973 17.52831418561069 34-35 8.618512181210788 19.970667318504034 57.280208587957304 14.130611912327872 36-37 6.2963415627800865 27.371058420923976 32.432982970748796 33.89961704554714 38-39 17.155544691599445 13.808767212580461 29.66267416279638 39.37301393302371 40-41 13.590809093131265 19.225128330481546 37.067139248757435 30.116923327629756 42-43 32.496129715635945 14.682636682147804 41.202232542980525 11.61900105923572 44-45 15.725576468671065 22.217469241424265 31.76688666177789 30.29006762812678 46-47 33.34962926749776 8.911838996170456 24.07927971970993 33.659252016621856 48-49 26.666259268312558 7.485944756783184 26.816996659333498 39.03079931557076 50-51 10.178847877454574 7.455389880224884 43.216817404057686 39.14894483826286 52-53 16.54852114397458 7.386132160026074 56.77096064531899 19.294386050680355 54-55 12.94100871832478 7.600016295934164 45.02159211276787 34.437382872973195 56-57 31.681333007414654 7.705939868002934 32.272060620875095 28.340666503707325 58-59 15.124663896357859 30.691355006925775 42.102582905565065 12.081398191151308 60-61 20.471767294060133 57.033732583720365 14.6174529454901 7.8770471767294055 62-63 7.09484233683696 77.39957630571172 7.557239468752547 7.948341888698769 64-65 5.367473315407806 79.90711317526277 6.885032184469974 7.8403813248594485 66-67 5.171922105434694 78.04326570520655 7.121323229854151 9.663488959504605 68-69 5.536543632363725 70.76713110079035 8.649067057769088 15.047258209076835 70-71 6.695591949808523 63.18952171433227 10.983459626823109 19.131426709036095 72-73 8.0603764360792 53.361036421412855 13.366739998370406 25.21184714413754 74-75 8.626660148293002 50.93497922268394 13.69469567342948 26.743664955593577 76 7.813900431842255 52.78253075857574 13.297482278171596 26.106086531410412 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 1.0 12 2.0 13 1.0 14 1.0 15 1.0 16 0.0 17 0.0 18 2.5 19 5.5 20 6.0 21 6.0 22 3.0 23 4.5 24 15.5 25 22.0 26 23.0 27 27.0 28 34.5 29 39.0 30 53.0 31 60.0 32 74.5 33 96.0 34 108.5 35 133.0 36 176.5 37 208.0 38 284.0 39 640.5 40 2067.0 41 4110.5 42 5008.0 43 4802.0 44 3973.5 45 2682.5 46 2014.0 47 1596.5 48 987.5 49 659.0 50 522.0 51 457.5 52 340.0 53 266.0 54 245.0 55 227.0 56 175.5 57 130.5 58 119.0 59 107.5 60 74.5 61 55.5 62 58.0 63 49.5 64 41.0 65 31.5 66 23.5 67 25.0 68 18.0 69 8.0 70 3.0 71 1.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 24546.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.19384013688585 #Duplication Level Percentage of deduplicated Percentage of total 1 71.17602996254682 19.355495803796952 2 16.98876404494382 9.239794671229529 3 5.49812734082397 4.485455878758249 4 2.2322097378277155 2.4280941904994706 5 1.2134831460674158 1.6499633341481301 6 0.599250936329588 0.9777560498655585 7 0.2696629213483146 0.5133219261794182 8 0.22471910112359553 0.48887802493277926 9 0.22471910112359553 0.5499877780493767 >10 1.2284644194756553 6.758738694695674 >50 0.0898876404494382 1.947364132648904 >100 0.1947565543071161 9.7245987126212 >500 0.0149812734082397 2.8803063635622914 >1k 0.0449438202247191 39.00024443901246 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 4150 16.90703169559195 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT 3988 16.2470463619327 RNA PCR Primer, Index 35 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 1435 5.846166381487819 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 707 2.8803063635622914 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 409 1.6662592683125559 No Hit TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 352 1.434042206469486 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT 217 0.8840544284201092 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 194 0.7903528069746599 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 190 0.7740568728102338 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 160 0.6518373665770391 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 141 0.5744316792960156 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 140 0.5703576957549091 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT 129 0.5255438768027377 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT 125 0.5092479426383117 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 115 0.46850810722724684 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT 109 0.4440642059806078 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT 106 0.4318422553572883 No Hit CTTATCACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 92 0.3748064857817974 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT 87 0.35443656807626495 No Hit CTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 85 0.346288600994052 No Hit TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 80 0.32591868328851953 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 75 0.30554876558298705 No Hit CTTATACACATCTCCGAGCCCACGCGACTGGGTTTCATCTCGTATGCCGT 59 0.24036502892528316 No Hit ATACACATCTCCGAGCCCACGAGACTGGGCTTCATCTCGTATGCCGTCTT 48 0.1955512099731117 TruSeq Adapter, Index 11 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGCCTGGGTTTCATCTCGTATGCCGT 44 0.17925527580868575 No Hit CTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 43 0.17518129226757925 No Hit TCTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 42 0.17110730872647273 No Hit ACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCT 38 0.15481137456204677 RNA PCR Primer, Index 35 (95% over 23bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC 37 0.15073739102094028 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTC 36 0.14666340747983378 No Hit CTTATATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 35 0.14258942393872728 No Hit TCTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 34 0.1385154403976208 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTAGTATGCCGT 32 0.13036747331540782 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCCTCTCGTATGCCGTCTT 31 0.1262934897743013 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCG 31 0.1262934897743013 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTCTGCCGTCTT 31 0.1262934897743013 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTC 28 0.11407153915098184 No Hit CTTATACACATATCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 28 0.11407153915098184 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTC 27 0.10999755560987534 No Hit CCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 26 0.10592357206876885 No Hit ATACACATCTCCGAGCCCACGAGACTGGGATTCATCTCGTATGCCGTCTT 26 0.10592357206876885 RNA PCR Primer, Index 39 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCCT 26 0.10592357206876885 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008147967082212987 0.0 2 0.0 0.0 0.0 0.008147967082212987 0.0 3 0.0 0.0 0.0 0.008147967082212987 0.0 4 0.0 0.0 0.0 0.008147967082212987 0.0 5 0.0 0.0 0.0 0.02036991770553247 0.0 6 0.0 0.0 0.0 0.02036991770553247 0.0 7 0.0 0.0 0.0 0.02036991770553247 0.0 8 0.0 0.0 0.0 0.02036991770553247 0.0 9 0.0 0.0 0.0 0.02036991770553247 0.0 10 0.0 0.0 0.0 0.03259186832885195 0.0 11 0.0 0.0 0.0 0.03259186832885195 0.0 12 0.0 0.0 0.0 0.03259186832885195 0.0 13 0.0 0.0 0.0 0.036665851869958445 0.0 14 0.0 0.0 0.0 0.04073983541106494 0.0 15 0.0 0.0 0.0 0.04481381895217143 0.0 16 0.0 0.0 0.0 0.04481381895217143 0.0 17 0.0 0.0 0.0 0.04481381895217143 0.0 18 0.0 0.0 0.0 0.048887802493277926 0.0 19 0.0 0.0 0.0 0.0651837366577039 0.0 20 0.0 0.0 0.0 0.0651837366577039 0.0 21 0.0 0.0 0.0 0.0692577201988104 0.0 22 0.0 0.0 0.0 0.0692577201988104 0.0 23 0.0 0.0 0.0 0.0692577201988104 0.0 24 0.0 0.0 0.0 0.07740568728102339 0.0 25 0.0 0.0 0.0 0.07740568728102339 0.0 26 0.0 0.0 0.0 0.07740568728102339 0.0 27 0.0 0.0 0.0 0.10999755560987534 0.0 28 0.0 0.0 0.0 0.10999755560987534 0.0 29 0.0 0.0 0.0 0.10999755560987534 0.0 30 0.0 0.0 0.0 0.1344414568565143 0.0 31 0.0 0.0 0.0 0.15073739102094028 0.0 32 0.0 0.0 0.0 0.17518129226757925 0.0 33 0.0 0.0 0.0 0.1996251935142182 0.0 34 0.0 0.0 0.0 0.23629104538417664 0.0 35 0.0 0.0 0.0 0.29332681495966756 0.0 36 0.0 0.0 0.0 0.3911024199462234 0.0 37 0.0 0.0 0.0 0.4807300578505663 0.0 38 0.0 0.0 0.0 0.6355414324126131 0.0 39 0.0 0.0 0.0 0.7822048398924468 0.0 40 0.0 0.0 0.0 0.9329422309133871 0.0 41 0.0 0.0 0.0 1.1040495396398597 0.0 42 0.0 0.0 0.0 1.3647844862706755 0.0 43 0.0 0.0 0.0 1.6458893506070236 0.0 44 0.0 0.0 0.0 1.8903283630734131 0.0 45 0.0 0.0 0.0 2.167359243868655 0.0 46 0.0 0.0 0.0 2.334392569054021 0.0 47 0.0 0.0 0.0 2.5462397131915586 0.0 48 0.0 0.0 0.0 2.7254949890002442 0.0 49 0.0 0.0 0.0 2.957712050843315 0.0 50 0.0 0.0 0.0 3.1369673266520004 0.0 51 0.0 0.0 0.0 3.3040006518373666 0.0 52 0.0 0.0 0.0 3.5769575490915018 0.0 53 0.0 0.0 0.0 3.752138841359081 0.0 54 0.0 0.0 0.0 3.935468100708873 0.0 55 0.0 0.0 0.0 4.0943534588120265 0.0 56 0.0 0.0 0.0 4.293978652326245 0.0 57 0.0 0.0 0.0 4.5017518129226755 0.0 58 0.0 0.0 0.0 4.701377006436894 0.0 59 0.0 0.0 0.0 4.852114397457834 0.0 60 0.0 0.0 0.0 5.023221706184307 0.0 61 0.0 0.0 0.0 5.226920883239631 0.0 62 0.0 0.0 0.0 5.3206225046850815 0.0 63 0.0 0.0 0.0 5.512099731117086 0.0 64 0.0 0.0 0.0 5.601727369021429 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACACC 25 2.758834E-6 70.00001 70 GGGTTTA 15 0.002198935 70.0 30 ATCTAGT 15 0.002198935 70.0 37 CATCTAG 15 0.002198935 70.0 36 TTATCAC 15 0.002198935 70.0 2 TGTTTAA 15 0.002198935 70.0 5 GGTTTAA 15 0.002198935 70.0 31 GTTTAAT 15 0.002198935 70.0 32 TAATCTC 15 0.002198935 70.0 35 CTAGTAT 15 0.002198935 70.0 39 CTTAATA 35 3.5161065E-9 70.0 1 TAATACA 35 3.5161065E-9 70.0 3 TCATCTA 15 0.002198935 70.0 35 TTAATAC 35 3.5161065E-9 70.0 2 TTTAATC 15 0.002198935 70.0 33 CTTTACA 215 0.0 68.37209 1 TTTACAC 215 0.0 66.74419 2 CTATACA 65 0.0 64.61539 1 TTACACA 230 0.0 62.391304 3 AATACAC 40 1.0104486E-8 61.25 4 >>END_MODULE