FastQCFastQC Report
Wed 25 May 2016
SRR1780413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162309
Sequences flagged as poor quality0
Sequence length50
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCG1650.10165794872742731No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1630.10042573116709486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCAC357.310078E-637.670192
GTTCGTC250.002342530535.213081
CTTTAGT250.00235675635.1696788
TTATACG250.002360323235.158842
TTAGACA401.8249792E-532.9614142
TATACGC353.2277653E-431.3918253
TTTACAC2050.031.0855622
CTTTACA2050.030.0599481
TGTATGG300.00566853929.39865919
ATTTAGT300.005728771429.33518611
CGAAACT1200.029.30806425
GACATGT300.005754739529.3080649
TAGACAC454.080568E-529.2990363
TATACAG300.00576341629.2990363
TATAACA300.00576341629.2990363
TTACACA2250.029.2990363
GAAACTC1150.028.6709326
CTTAAAC406.9905096E-427.510221
TAATACA407.041126E-427.4763094
ATAACAC407.041126E-427.4763094
Produced by FastQC (version 0.11.5)