FastQCFastQC Report
Wed 25 May 2016
SRR1780412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21595
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACA150.002196182769.99303420
TGCAGAC150.002196182769.99303421
GACTAAC150.002196182769.99303417
AATTATC200.00686298952.4947835
CTCCGAC200.00686298952.4947819
ACCACCA1051.9244893E-523.3310132
CACCACC1904.3837645E-1022.2579921
AACACAC1653.155985E-516.968012
ACCACCC1450.00454585414.4813182
CAACACA1951.239809E-414.4581831
CACCACA1800.001129783613.7051531
ACACACC2203.661636E-412.7260073
CACATCT2802.1500049E-411.2488814
ATACACA3150.00689016668.9503041
Produced by FastQC (version 0.11.5)