FastQCFastQC Report
Wed 25 May 2016
SRR1780412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21595
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT348516.137994906228293TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT333015.420236165779116No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC13126.075480435285946No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC5732.6533919888863164No Hit
TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT3201.4818244964112062No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG2801.2965964343598055No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT1880.8705718916415837No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC1830.8474183838851587No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC1620.7501736513081732No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG1610.7455429497568882No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG1550.7177587404491781No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT1460.6760824264876129No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT1220.5649455892567724No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT1210.5603148877054873No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT1180.5464227830516324No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC1150.5325306783977772No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT960.4445473489233619No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC890.4121324380643668No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG690.31951840703866635No Hit
CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC580.26858068997453116No Hit
CTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT570.26394998842324613No Hit
TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC540.2500578837693911No Hit
CTTATATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC530.24542718221810603No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGT510.23616577911553602No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG440.20375086825654087No Hit
CTTATACACATCTCCGAGCCCACGCGACGTGTGGTGATCTCGTATGCCGT430.19912016670525584No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTC400.18522806205140077No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTGTGGTGATCTCGTATGCCGT390.18059736050011577No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTC380.17596665894883076No Hit
ACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCT330.15281315119240566RNA PCR Primer, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGTGTGGTGCTCTCGTATGCCGTCTT330.15281315119240566No Hit
ATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGTCTT320.14818244964112062Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTGGTGATCTCGTATGCCGT310.14355174808983562No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTCTGCCGTCTT290.13429034498726558No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTC280.12965964343598055No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT280.12965964343598055No Hit
ATACACATCTCCGAGCCCACGAGCCGTGTGGTGATCTCGTATGCCGTCTT270.12502894188469554TruSeq Adapter, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGAAGTGTGGTGATCTCGTATGCCGTCTT270.12502894188469554Illumina PCR Primer Index 5 (95% over 21bp)
TCTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT260.12039824033341053No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTC250.11576753878212549No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCG230.10650613567955544No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTC220.10187543412827044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACA252.7474834E-670.000013
TTATAAC252.7474834E-670.000012
TTATTAC207.7536926E-570.02
ATTACAC207.7536926E-570.04
TCTCGTC150.002195316870.038
CTTATTA207.7536926E-570.01
CGAGCAC150.002195316870.015
CCGAGCA150.002195316870.014
TATTACA207.7536926E-570.03
GTGGTGC150.002195316870.028
TCTTTAT353.4888217E-970.01
GCACACG150.002195316870.018
TTTACAC1300.067.307692
CTTTACA1250.067.21
CTATACA600.064.1666641
CTTAATA352.8402792E-760.01
AATACAC352.8402792E-760.04
TTAATAC352.8402792E-760.02
ATAACAC308.105602E-658.3333324
CTTATAA308.105602E-658.3333321
Produced by FastQC (version 0.11.5)