##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780412_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21595 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92113915258162 34.0 34.0 34.0 32.0 34.0 2 33.335586941421624 34.0 34.0 34.0 33.0 34.0 3 33.530030099560086 34.0 34.0 34.0 34.0 34.0 4 33.56587172956703 34.0 34.0 34.0 34.0 34.0 5 33.642787682333875 34.0 34.0 34.0 34.0 34.0 6 37.38263486918268 38.0 38.0 38.0 37.0 38.0 7 36.99661958786756 38.0 37.0 38.0 36.0 38.0 8 37.02829358647835 38.0 37.0 38.0 36.0 38.0 9 36.7059504514934 38.0 37.0 38.0 34.0 38.0 10-11 36.45667978698773 38.0 37.0 38.0 34.0 38.0 12-13 36.182935864783516 38.0 37.0 38.0 33.5 38.0 14-15 36.22414910858995 38.0 37.0 38.0 34.0 38.0 16-17 36.19650382032878 38.0 36.5 38.0 32.5 38.0 18-19 36.365709655012736 38.0 37.0 38.0 34.0 38.0 20-21 36.13665200277842 38.0 37.0 38.0 32.5 38.0 22-23 36.10803426719148 38.0 36.5 38.0 32.5 38.0 24-25 34.92479740680713 37.5 34.5 38.0 28.0 38.0 26-27 34.85705024311183 37.0 34.5 38.0 28.0 38.0 28-29 34.75941190090299 37.0 34.0 38.0 27.0 38.0 30-31 34.19608705718916 37.0 34.0 38.0 25.0 38.0 32-33 34.98645519796249 37.0 34.5 38.0 29.0 38.0 34-35 34.006181986570965 37.0 34.0 38.0 24.0 38.0 36-37 33.73003009956008 37.0 34.0 38.0 24.0 38.0 38-39 32.43878212549201 37.0 31.5 38.0 11.5 38.0 40-41 33.806922898819174 37.0 34.0 38.0 24.0 38.0 42-43 35.19256772401019 37.0 35.0 38.0 29.0 38.0 44-45 35.53718453345682 37.5 36.0 38.0 31.0 38.0 46-47 35.62544570502431 38.0 36.0 38.0 31.0 38.0 48-49 35.16841861542024 37.5 35.0 38.0 29.0 38.0 50-51 35.22528363047002 37.5 35.5 38.0 31.0 38.0 52-53 34.95278999768465 37.0 35.0 38.0 27.0 38.0 54-55 35.295508219495254 37.5 36.0 38.0 29.0 38.0 56-57 35.1926140310257 38.0 36.0 38.0 27.0 38.0 58-59 35.02746006019912 37.0 36.0 38.0 27.0 38.0 60-61 34.920838156980786 37.0 35.5 38.0 28.5 38.0 62-63 35.52190321833758 37.5 36.0 38.0 31.0 38.0 64-65 35.44873813382728 38.0 36.0 38.0 31.0 38.0 66-67 33.693007640657555 37.0 34.0 38.0 22.5 38.0 68-69 29.92435749015976 35.0 24.0 38.0 11.0 38.0 70-71 26.849965269738366 29.0 16.0 37.0 11.0 38.0 72-73 24.015188701088213 23.5 11.0 35.5 11.0 38.0 74-75 23.653044686269972 24.0 11.0 35.5 11.0 38.0 76 23.65927297985645 24.0 11.0 35.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19748555910860688 1101 2 0.20803092772516862 1101 3 -0.45182059724831447 1101 4 0.23262877479174193 1101 5 0.16955281615636864 1101 6 -1.6393992765101402 1101 7 0.15980025211504056 1101 8 0.5916169058369221 1101 9 0.41671363802588957 1101 10-11 0.46371975389799047 1101 12-13 0.9316424654247228 1101 14-15 0.8899722875468044 1101 16-17 -0.29702064350355784 1101 18-19 -1.0213084291412784 1101 20-21 -0.5520331237841631 1101 22-23 0.34713539512505776 1101 24-25 -2.0469615863449704 1101 26-27 0.6220073110539275 1101 28-29 1.1660178896789972 1101 30-31 1.2954155759286579 1101 32-33 0.06911222657964089 1101 34-35 -0.3898036653836314 1101 36-37 -1.0657017653215561 1101 38-39 -0.27166816875319455 1101 40-41 1.6454869180821703 1101 42-43 0.4726607270412586 1101 44-45 -0.015978244212952575 1101 46-47 0.3377389852850712 1101 48-49 -1.910406385739499 1101 50-51 -1.299085138739315 1101 52-53 -1.9125390721380242 1101 54-55 -2.711456405480561 1101 56-57 -3.088072165670667 1101 58-59 -2.9912457887182455 1101 60-61 -2.630921362621507 1101 62-63 -1.0519344522709417 1101 64-65 -2.5802889187259765 1101 66-67 -3.104515818088636 1101 68-69 -1.867692362505796 1101 70-71 -0.13117256539870326 1101 72-73 -0.6162480226846334 1101 74-75 -1.8319608393825355 1101 76 -1.859623292817382 1102 1 -1.329515091509485 1102 2 -0.5583971789761932 1102 3 -0.003544735179346503 1102 4 0.5156476427162673 1102 5 -0.12452655925027045 1102 6 0.35539577879239914 1102 7 0.38654065549825134 1102 8 0.4943495018030859 1102 9 0.5644039438163944 1102 10-11 0.7830105801829603 1102 12-13 0.344134332698637 1102 14-15 -0.6781799570855966 1102 16-17 -0.38631797596940487 1102 18-19 -1.176318188412587 1102 20-21 0.5945186003537728 1102 22-23 0.6631731309741156 1102 24-25 -5.119830812109441 1102 26-27 -0.85635963754725 1102 28-29 -0.08577131006074978 1102 30-31 2.3878033443086153 1102 32-33 0.04194892143976148 1102 34-35 1.0814064842585296 1102 36-37 -2.202819527195331 1102 38-39 -0.361128155740829 1102 40-41 -0.26212531353786517 1102 42-43 -0.5632859222755968 1102 44-45 0.6042234473940766 1102 46-47 1.0916426938081685 1102 48-49 -3.674719983657525 1102 50-51 -0.7224748589735341 1102 52-53 -0.4863842250332766 1102 54-55 -0.02554228706807038 1102 56-57 0.4132290705036965 1102 58-59 -0.4203284172153161 1102 60-61 -7.521129560409403 1102 62-63 -3.4608479200653406 1102 64-65 0.15312032005085996 1102 66-67 2.010318274299131 1102 68-69 0.8457103700901705 1102 70-71 -1.3829617651384538 1102 72-73 -2.569078211363877 1102 74-75 -1.2133856929934623 1102 76 -2.2922843207939607 1103 1 0.8186530119387925 1103 2 0.5299648899893228 1103 3 -0.04557059724831447 1103 4 0.4843976427162673 1103 5 0.1082320614393879 1103 6 0.43459836499929594 1103 7 0.6618639313603225 1103 8 0.8445650190444667 1103 9 0.529921185195704 1103 10-11 0.6143683388036507 1103 12-13 -0.03032687419791813 1103 14-15 -0.3427812501890415 1103 16-17 0.5686927998926663 1103 18-19 0.9602658633115553 1103 20-21 0.39327937621583686 1103 22-23 0.6319231309741156 1103 24-25 -4.385994605212893 1103 26-27 -1.0551742927196628 1103 28-29 1.3062006726978694 1103 30-31 1.5874261891362025 1103 32-33 1.966517886957 1103 34-35 1.1137340704654264 1103 36-37 -0.509392803057402 1103 38-39 0.268720982190203 1103 40-41 0.398435031289722 1103 42-43 -0.04900790503421604 1103 44-45 1.1408613784285606 1103 46-47 1.262709504152994 1103 48-49 -2.992069121588557 1103 50-51 0.1239691065437043 1103 52-53 0.8433571542770721 1103 54-55 1.5092098681043424 1103 56-57 1.412959673951974 1103 58-59 -0.10297927928428408 1103 60-61 -4.544836456961129 1103 62-63 -1.089080678686031 1103 64-65 0.39288325108534394 1103 66-67 -1.2380653463905205 1103 68-69 -1.1125331643925875 1103 70-71 1.6574477176201654 1103 72-73 -2.490953211363877 1103 74-75 -1.240864141269327 1103 76 1.1527587826543169 1104 1 0.278307862685061 1104 2 0.10552459148185989 1104 3 -0.30256686590502824 1104 4 -0.5589792229553723 1104 5 -0.4029619684113612 1104 6 -0.5087318588812977 1104 7 -0.7712050984904266 1104 8 0.22749412352207798 1104 9 0.7985779016136121 1104 10-11 -0.23101412388291465 1104 12-13 0.4419212601304352 1104 14-15 0.6382122199602165 1104 16-17 0.7805631357135567 1104 18-19 0.8253544827145376 1104 20-21 0.07483254785762483 1104 22-23 0.6669044742576986 1104 24-25 0.9241733052348664 1104 26-27 1.7953387669818355 1104 28-29 1.9348154115038412 1104 30-31 1.2298002563003791 1104 32-33 0.6540178869569999 1104 34-35 -0.3909301086390542 1104 36-37 2.0108963760470786 1104 38-39 2.3398497135334893 1104 40-41 2.132110404424054 1104 42-43 0.636276609891155 1104 44-45 0.8171673485778115 1104 46-47 1.3516787205709022 1104 48-49 0.3295260276651746 1104 50-51 1.0537732110213156 1104 52-53 0.6726481990531923 1104 54-55 0.412311547208823 1104 56-57 0.9016490396236136 1104 58-59 -0.10741024943354205 1104 60-61 1.1053501102030552 1104 62-63 0.870340963105015 1104 64-65 0.8959149675032521 1104 66-67 -0.7187090031069374 1104 68-69 2.9391226191895043 1104 70-71 1.3465801803067308 1104 72-73 -0.14370507703551993 1104 74-75 0.6241078736560439 1104 76 0.9573296781767056 1105 1 0.9414208690816466 1105 2 0.5317506042750395 1105 3 0.3481794027516827 1105 4 -0.027209500140877196 1105 5 0.15823206143938506 1105 6 0.24308050785644042 1105 7 0.6471317885031809 1105 8 0.10617216190161116 1105 9 1.1013497566242734 1105 10-11 0.4674933388036564 1105 12-13 -0.29059473134077507 1105 14-15 0.6779776783823834 1105 16-17 0.7957017284640884 1105 18-19 0.4162926490258414 1105 20-21 0.4336811619301173 1105 22-23 0.7274588452598323 1105 24-25 1.8791839662156775 1105 26-27 0.9586649929946205 1105 28-29 1.1524060298407264 1105 30-31 1.8242565462790594 1105 32-33 1.4254464583855722 1105 34-35 -1.8523373581060056 1105 36-37 -0.470553517343113 1105 38-39 0.6548816964759183 1105 40-41 0.6671850312897192 1105 42-43 0.919965309251495 1105 44-45 1.592647092714273 1105 46-47 1.7901648612958496 1105 48-49 -0.13358697873141523 1105 50-51 1.2610226779722709 1105 52-53 0.30942858284849706 1105 54-55 1.552290225247198 1105 56-57 0.7770221739519769 1105 58-59 0.9742528635728576 1105 60-61 0.8855206858960116 1105 62-63 0.04038360702825372 1105 64-65 1.3986868225139162 1105 66-67 1.8347025107523365 1105 68-69 0.4506364784645527 1105 70-71 -0.30907013952269224 1105 72-73 -0.8047924970781644 1105 74-75 -0.37791771269790075 1105 76 0.5929373540828884 1106 1 -1.17887988279805 1106 2 0.436763135603357 1106 3 0.05695133257624718 1106 4 0.41038448482152745 1106 5 0.2880566228428947 1106 6 0.15636371587648767 1106 7 -0.27316896337651997 1106 8 0.4668237909742885 1106 9 -0.522710393751666 1106 10-11 0.6727564966983905 1106 12-13 1.5604077749248901 1106 14-15 0.057300986653068264 1106 16-17 1.2829197735768716 1106 18-19 0.8507538019080485 1106 20-21 0.8777914376193436 1106 22-23 0.6500152362372731 1106 24-25 -0.16066565784447562 1106 26-27 1.1238279002627891 1106 28-29 1.4417544007680476 1106 30-31 0.9600961452765517 1106 32-33 1.075070518535945 1106 34-35 1.9218481055531456 1106 36-37 3.1103988636092623 1106 38-39 1.3748065085059906 1106 40-41 2.4856060839212972 1106 42-43 1.931118191457017 1106 44-45 1.6397648872004922 1106 46-47 1.4777588462582543 1106 48-49 1.8568892117447788 1106 50-51 0.5650327030349303 1106 52-53 1.0220852244525105 1106 54-55 0.0556736838938221 1106 56-57 0.5200046300923304 1106 58-59 1.4559571242244829 1106 60-61 2.1163477535651865 1106 62-63 1.3204587949981743 1106 64-65 -0.8376540296164094 1106 66-67 1.5363315834340412 1106 68-69 2.3305863531512756 1106 70-71 0.5393007877956038 1106 72-73 0.19407968337296566 1106 74-75 0.9978968236429537 1106 76 1.6829122914262484 1107 1 0.4381241657849486 1107 2 -0.23638126385682767 1107 3 -0.128743674171389 1107 4 0.20795533502396069 1107 5 0.15823206143938506 1107 6 0.37714644192237046 1107 7 0.6053735467449357 1107 8 -0.5213003655709159 1107 9 -0.9008480455735253 1107 10-11 0.35980103111133843 1107 12-13 0.10885581810977385 1107 14-15 0.3609447113494255 1107 16-17 0.444053376815738 1107 18-19 0.6113475940807902 1107 20-21 0.5018130300619887 1107 22-23 -0.7099037921028071 1107 24-25 -1.1230138359821282 1107 26-27 -0.934741600411968 1107 28-29 0.8452631726978694 1107 30-31 1.0055752275977383 1107 32-33 1.4617101946493065 1107 34-35 -0.6545351603038085 1107 36-37 1.7052706584810622 1107 38-39 1.0752113668055898 1107 40-41 -0.37512266101796854 1107 42-43 0.9018334411196278 1107 44-45 0.58605368612087 1107 46-47 1.083022004152994 1107 48-49 1.1389404937960563 1107 50-51 1.5742094911590883 1107 52-53 0.6077802312001523 1107 54-55 0.983608906565884 1107 56-57 1.527022173951977 1107 58-59 0.6313957207157159 1107 60-61 1.1657404661157926 1107 62-63 -0.08763837099372296 1107 64-65 1.5887967126238038 1107 66-67 -1.3185941925443672 1107 68-69 0.9385485663766424 1107 70-71 -1.367311897764452 1107 72-73 -1.4844628267484943 1107 74-75 -0.6641814489616351 1107 76 -0.3092604481149124 1108 1 0.13266510871298465 1108 2 0.26585198676351496 1108 3 0.12882456404200582 1108 4 0.48338957820013917 1108 5 0.21307077111680428 1108 6 -0.23526050596844783 1108 7 -0.09065622992999778 1108 8 -0.6624914325684372 1108 9 -0.05072397609461632 1108 10-11 0.16426753235203506 1108 12-13 1.5084836096730427 1108 14-15 0.9397288304561187 1108 16-17 -0.008676151720237613 1108 18-19 0.006384814924459192 1108 20-21 1.0447410697642212 1108 22-23 0.009947324522499912 1108 24-25 0.9496908786580747 1108 26-27 -0.7109202604615987 1108 28-29 -0.533769085366643 1108 30-31 0.38808143107169 1108 32-33 1.4604694998602241 1108 34-35 0.014943747884778702 1108 36-37 2.153409616297438 1108 38-39 2.3518863047708436 1108 40-41 1.7704108377413377 1108 42-43 0.19922798206255266 1108 44-45 1.2719097655253364 1108 46-47 1.2685058751207379 1108 48-49 1.5235558784114431 1108 50-51 1.1476586226727363 1108 52-53 -0.05785252314228728 1108 54-55 -0.6548526318956576 1108 56-57 0.5366995933068068 1108 58-59 0.6072021723286127 1108 60-61 2.078147414006615 1108 62-63 1.480475772926873 1108 64-65 0.6030647026982479 1108 66-67 1.0356241697385116 1108 68-69 2.1593922388332203 1108 70-71 0.23355658858790918 1108 72-73 0.5518895305716072 1108 74-75 0.2999624716338971 1108 76 1.2505410407188329 1109 1 -2.042706115045334 1109 2 0.20952838205281665 1109 3 0.16722702179930593 1109 4 0.24580637287499485 1109 5 0.1677558709631981 1109 6 0.06530273007866327 1109 7 0.05348099485238578 1109 8 0.41252136825081465 1109 9 1.085476740751261 1109 10-11 0.31352508483539765 1109 12-13 0.8975005067544615 1109 14-15 0.8597237101284136 1109 16-17 1.2687176014799704 1109 18-19 0.8837529664861634 1109 20-21 1.6701890984380583 1109 22-23 0.9925382103391911 1109 24-25 -0.9668477798160708 1109 26-27 0.02691896124859028 1109 28-29 -0.9721971447624469 1109 30-31 -0.5741561521336394 1109 32-33 0.25719249013160095 1109 34-35 0.4381388323701856 1109 36-37 1.7691290223394205 1109 38-39 -0.09511830352408879 1109 40-41 0.9425818566865445 1109 42-43 1.3985367378229228 1109 44-45 1.2370915371587188 1109 46-47 0.829053750184741 1109 48-49 1.1902225450781074 1109 50-51 -0.29294557599597937 1109 52-53 -0.5485079250880034 1109 54-55 0.25784578080275367 1109 56-57 0.049244396174195515 1109 58-59 -0.2574931681731698 1109 60-61 1.1029810033563336 1109 62-63 0.4253042419488935 1109 64-65 1.024083647910743 1109 66-67 0.5354961615459857 1109 68-69 -0.9668238389957651 1109 70-71 1.000453670001118 1109 72-73 -1.8706655129511809 1109 74-75 -2.2882351730153587 1109 76 -3.703888042742509 1110 1 -0.7907219880612075 1110 2 -0.20441011001067722 1110 3 0.12630440275168553 1110 4 0.3593976427162673 1110 5 0.2644820614393879 1110 6 0.012723364999295939 1110 7 0.6306139313603225 1110 8 0.5008150190444667 1110 9 0.639296185195704 1110 10-11 -0.2684441611963493 1110 12-13 0.7040481258020819 1110 14-15 1.1494062498109585 1110 16-17 0.9280677998926663 1110 18-19 0.2571408633115553 1110 20-21 0.8698418762158369 1110 22-23 -1.0946393690258844 1110 24-25 -0.4406821052128933 1110 26-27 1.8042007072803372 1110 28-29 1.0874506726978694 1110 30-31 -1.0531988108637975 1110 32-33 1.419642886957 1110 34-35 1.2543590704654264 1110 36-37 2.631232196942598 1110 38-39 1.393720982190203 1110 40-41 -0.546877468710278 1110 42-43 0.693179594965784 1110 44-45 1.4611738784285606 1110 46-47 1.200209504152994 1110 48-49 1.7501183784114431 1110 50-51 1.1864691065437043 1110 52-53 1.3902321542770721 1110 54-55 0.34514736810434243 1110 56-57 0.8817096739519741 1110 58-59 -1.165479279284284 1110 60-61 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-3.0144376126176198 2116 60-61 -2.1698364569611286 2116 62-63 1.004669321313969 2116 64-65 -1.4560750822479847 2116 66-67 0.15255965360947954 2116 68-69 -1.7818039977259232 2116 70-71 0.4543227176201654 2116 72-73 -0.13157821136387682 2116 74-75 -0.8814891412693271 2116 76 0.6631754493209847 2117 1 1.062538881504011 2117 2 0.7174648899893228 2117 3 0.4829620114473343 2117 4 -0.4191349659793815 2117 5 0.24953640926547394 2117 6 0.43935379978190525 2117 7 0.6204237139690179 2117 8 0.34592371469663874 2117 9 -3.0570353365434286 2117 10-11 -1.0738110090224424 2117 12-13 -2.635625787241402 2117 14-15 0.008101901984865378 2117 16-17 -1.4024349174986384 2117 18-19 -1.1868129410362727 2117 20-21 -1.2259461672624283 2117 22-23 0.15230356575672488 2117 24-25 -2.6509402573868073 2117 26-27 -4.574875379676186 2117 28-29 -2.980823783823869 2117 30-31 -0.4937558760811882 2117 32-33 -4.498156026086477 2117 34-35 0.19797320090020776 2117 36-37 -2.5019199769704414 2117 38-39 -1.0050561917228436 2117 40-41 -1.382814968710278 2117 42-43 -0.5921464919907322 2117 44-45 -1.649899491136658 2117 46-47 -1.6126301697600525 2117 48-49 -3.7481832520233382 2117 50-51 -2.02344937171717 2117 52-53 -0.2734906718098813 2117 54-55 -0.9918091536347831 2117 56-57 0.5552830435171927 2117 58-59 -1.8577347140668934 2117 60-61 -2.3682060221785193 2117 62-63 -1.1366350265121241 2117 64-65 -2.9923069663059607 2117 66-67 -2.309396868129653 2117 68-69 -1.3356989252621503 2117 70-71 -0.7888838041189672 2117 72-73 0.9635304842882952 2117 74-75 0.2652499891654543 2117 76 0.5780305217847506 2118 1 0.5190606206344412 2118 2 0.1305083682501902 2118 3 0.20035331579516225 2118 4 -0.13652627032720943 2118 5 0.3582320614393879 2118 6 -0.2780375045659227 2118 7 0.7725976270124946 2118 8 0.7154889320879434 2118 9 -3.470078814804296 2118 10-11 -0.8020718785876539 2118 12-13 -2.4399736133283554 2118 14-15 0.029841032419660962 2118 16-17 -4.598087091411681 2118 18-19 -0.925943375818882 2118 20-21 -0.8346418194363352 2118 22-23 0.8805644353219435 2118 24-25 1.3273206121784114 2118 26-27 -3.1509623361979244 2118 28-29 -1.8395194359977864 2118 30-31 -2.3741906586898835 2118 32-33 0.045322234783085946 2118 34-35 0.3175384182915195 2118 36-37 -4.87148519436175 2118 38-39 -1.842012713461969 2118 40-41 -0.991510620884192 2118 42-43 -0.29866823112116947 2118 44-45 -2.965116882441002 2118 46-47 -0.9169779958470059 2118 48-49 0.001816747976661759 2118 50-51 -2.5451885021519516 2118 52-53 -3.2843602370272755 2118 54-55 -1.9374613275478296 2118 56-57 0.12050043482153683 2118 58-59 -1.1838216705886282 2118 60-61 -2.802988630874175 2118 62-63 -1.9409828525990775 2118 64-65 -0.39448087934943743 2118 66-67 -0.6789620855209577 2118 68-69 -2.172655447001283 2118 70-71 -0.7127968475972253 2118 72-73 1.5939652668969941 2118 74-75 0.3522065109045869 2118 76 0.14324791308909823 2119 1 0.8950888227496065 2119 2 -1.5528053802809474 2119 3 -0.2356043810320969 2119 4 -0.214082087013459 2119 5 -0.6687949655876366 2119 6 -1.3268036620277286 2119 7 -1.5775786362072424 2119 8 -0.5042525485230982 2119 9 -0.5781869229124013 2119 10-11 -0.7973715260612124 2119 12-13 -0.6996680904141357 2119 14-15 -0.7222153718106625 2119 16-17 -2.140977808215446 2119 18-19 -1.4626648799316868 2119 20-21 -2.9574385291895666 2119 22-23 -0.26250254470156165 2119 24-25 1.0455932326249453 2119 26-27 0.7999777343073617 2119 28-29 -0.15473682730213056 2119 30-31 1.5373839594064762 2119 32-33 -0.06219832925921054 2119 34-35 0.05461244884380534 2119 36-37 0.06535381856422617 2119 38-39 0.04194733354155744 2119 40-41 -2.139571725467036 2119 42-43 -0.8118879726017809 2119 44-45 0.740523540590722 2119 46-47 -1.5250861039551182 2119 48-49 -1.7872549323993638 2119 50-51 0.28148599843559197 2119 52-53 0.35518147860139493 2119 54-55 0.27757980053677755 2119 56-57 -1.6459507990209943 2119 58-59 -0.5442799549599613 2119 60-61 -0.06003915966383033 2119 62-63 -1.0628982462535959 2119 64-65 -0.5349039110768175 2119 66-67 1.0410731671229954 2119 68-69 1.3294009572290335 2119 70-71 1.129153798701246 2119 72-73 4.593083950798285 2119 74-75 3.9056730208928343 2119 76 3.2566439177894537 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 4.0 23 8.0 24 32.0 25 55.0 26 126.0 27 262.0 28 434.0 29 717.0 30 1038.0 31 1569.0 32 2124.0 33 3060.0 34 3977.0 35 3581.0 36 1989.0 37 2618.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 12.303774021764298 28.705718916415833 9.942116230608937 49.04839083121093 2 6.126418152350081 8.4695531373003 72.94281083584163 12.461217874507987 3 5.468858532067608 33.822644130585786 51.266496874276456 9.442000463070155 4 4.352859458207918 34.67006251447094 28.784440842787685 32.19263718453346 5 3.9731419310025466 49.96063903681407 38.62005093771707 7.446168094466311 6 8.07131280388979 41.06043065524427 10.60893725399398 40.25931928687196 7 8.288955776800186 50.043991664737206 10.076406575596202 31.590645982866405 8 6.459828664042602 39.495253530909935 29.108589951377635 24.93632785366983 9 6.700625144709424 23.98240333410512 9.335494327390599 59.981477193794866 10-11 7.270201435517482 23.530909932854826 38.34452419541561 30.854364436212084 12-13 18.41398471868488 8.890946978467237 30.319518407038665 42.375549895809215 14-15 25.265107663811065 19.620282472794628 11.717990275526741 43.39661958786756 16-17 28.807594350544107 26.281546654318127 7.624450104190784 37.28640889094697 18-19 23.000694605232695 23.83885158601528 7.346608011113684 45.81384579763834 20-21 19.90043991664737 16.332484371382264 7.464690900671451 56.302384811298914 22-23 25.76522343134985 34.6839546191248 7.6175040518638575 31.933317897661496 24-25 28.53901366056958 40.22458902523732 8.198657096550127 23.037740217642973 26-27 35.19796249131743 30.252373234545033 18.90946978467238 15.640194489465154 28-29 39.182681176198194 12.998379254457051 26.911322065292893 20.907617504051863 30-31 52.55614725630933 7.983329474415374 29.736050011576754 9.724473257698541 32-33 48.08983561009493 8.492706645056726 36.17735586941422 7.240101875434128 34-35 33.34105116925214 20.74785830053253 38.369993053947674 7.5410974762676535 36-37 23.306320907617504 26.793239175735124 30.641352164852975 19.259087751794397 38-39 19.742996063903682 13.764760361194723 28.842324612178743 37.64991896272285 40-41 14.364436212086131 19.45589256772401 36.57328085204908 29.606390368140772 42-43 31.8082889557768 15.427182218106042 40.69692058346839 12.067608242648761 44-45 15.68187080342672 21.498031951840705 31.426256077795784 31.39384116693679 46-47 32.6140310257004 9.590182912711276 24.674693216022227 33.1210928455661 48-49 25.982866404260246 7.872192637184533 27.818939569344757 38.32600138921047 50-51 10.178282009724473 7.513313266959945 43.19981477193795 39.10858995137763 52-53 16.89974531141468 8.078258856216717 55.14934012502894 19.87265570733966 54-55 13.329474415373928 8.168557536466775 44.54040287103496 33.961565177124335 56-57 30.56494558925677 8.305163232229683 33.322528363047 27.80736281546654 58-59 15.417920815003471 31.36374160685344 40.483908312109286 12.734429266033803 60-61 19.86107895346145 56.91826811761982 14.570502431118316 8.650150497800418 62-63 7.091919425793007 75.49664274137533 8.47649918962723 8.934938643204445 64-65 5.742069923593425 77.77957860615882 7.612873350312572 8.86547811993517 66-67 5.711970363510072 75.95508219495254 7.793470710812687 10.539476730724704 68-69 6.221347534151424 68.85158601528131 9.330863625839314 15.596202824727945 70-71 7.390599675850891 61.30354248668674 11.166936790923824 20.13892104653855 72-73 8.70803426719148 52.23894420004631 13.461449409585551 25.591572123176658 74-75 9.35401713359574 49.6295438758972 13.970826580226905 27.045612410280157 76 8.724241722620977 51.887010882148644 13.040055568418616 26.34869182681176 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 1.0 18 0.5 19 1.0 20 1.0 21 0.0 22 5.0 23 8.5 24 8.0 25 9.0 26 14.5 27 23.5 28 30.5 29 34.0 30 32.0 31 45.0 32 67.0 33 74.0 34 106.0 35 150.5 36 174.0 37 185.0 38 209.0 39 301.5 40 612.5 41 1797.0 42 2739.0 43 3488.0 44 4127.0 45 3414.0 46 2811.0 47 2307.0 48 1436.5 49 858.5 50 647.0 51 523.0 52 355.5 53 286.0 54 260.0 55 236.0 56 179.5 57 149.0 58 151.0 59 151.0 60 134.0 61 107.0 62 97.0 63 88.5 64 68.5 65 44.5 66 26.5 67 21.0 68 16.0 69 5.5 70 0.5 71 1.0 72 0.5 73 1.0 74 1.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 21595.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.84463996295439 #Duplication Level Percentage of deduplicated Percentage of total 1 72.25878953283032 20.842787682333874 2 15.989725477604752 9.22435749015976 3 5.522555787445818 4.778884000926141 4 2.488360892599133 2.8710349617967124 5 1.1558837694654038 1.6670525584626072 6 0.38529458982180126 0.6668210233850429 7 0.33713276609407605 0.6807131280388978 8 0.24080911863862578 0.5556841861542023 9 0.09632364745545031 0.2500578837693911 >10 1.1398298282228287 6.144940958555221 >50 0.12843152994060042 2.4403797175272057 >100 0.19264729491090063 9.590182912711276 >500 0.016053941242575052 2.6533919888863164 >1k 0.04816182372772516 37.633711507293356 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT 3485 16.137994906228293 TruSeq Adapter, Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 3330 15.420236165779116 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 1312 6.075480435285946 No Hit CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 573 2.6533919888863164 No Hit TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT 320 1.4818244964112062 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 280 1.2965964343598055 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT 188 0.8705718916415837 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 183 0.8474183838851587 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 162 0.7501736513081732 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 161 0.7455429497568882 No Hit CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 155 0.7177587404491781 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT 146 0.6760824264876129 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT 122 0.5649455892567724 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT 121 0.5603148877054873 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT 118 0.5464227830516324 No Hit CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 115 0.5325306783977772 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT 96 0.4445473489233619 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 89 0.4121324380643668 No Hit CTTATTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 69 0.31951840703866635 No Hit CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 58 0.26858068997453116 No Hit CTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT 57 0.26394998842324613 No Hit TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 54 0.2500578837693911 No Hit CTTATATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 53 0.24542718221810603 No Hit CTTATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGT 51 0.23616577911553602 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 44 0.20375086825654087 No Hit CTTATACACATCTCCGAGCCCACGCGACGTGTGGTGATCTCGTATGCCGT 43 0.19912016670525584 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTC 40 0.18522806205140077 No Hit CTTATACACATCTCCGAGCCCACGAGCCGTGTGGTGATCTCGTATGCCGT 39 0.18059736050011577 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTC 38 0.17596665894883076 No Hit ACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCT 33 0.15281315119240566 RNA PCR Primer, Index 5 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACGTGTGGTGCTCTCGTATGCCGTCTT 33 0.15281315119240566 No Hit ATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGTCTT 32 0.14818244964112062 Illumina PCR Primer Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGTGGTGATCTCGTATGCCGT 31 0.14355174808983562 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTCTGCCGTCTT 29 0.13429034498726558 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTC 28 0.12965964343598055 No Hit TCTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 28 0.12965964343598055 No Hit ATACACATCTCCGAGCCCACGAGCCGTGTGGTGATCTCGTATGCCGTCTT 27 0.12502894188469554 TruSeq Adapter, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGAAGTGTGGTGATCTCGTATGCCGTCTT 27 0.12502894188469554 Illumina PCR Primer Index 5 (95% over 21bp) TCTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 26 0.12039824033341053 No Hit CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTC 25 0.11576753878212549 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCG 23 0.10650613567955544 No Hit CTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTC 22 0.10187543412827044 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0046307015512850195 0.0 6 0.0 0.0 0.0 0.0046307015512850195 0.0 7 0.0 0.0 0.0 0.0046307015512850195 0.0 8 0.0 0.0 0.0 0.009261403102570039 0.0 9 0.0 0.0 0.0 0.009261403102570039 0.0 10 0.0 0.0 0.0 0.009261403102570039 0.0 11 0.0 0.0 0.0 0.009261403102570039 0.0 12 0.0 0.0 0.0 0.009261403102570039 0.0 13 0.0 0.0 0.0 0.009261403102570039 0.0 14 0.0 0.0 0.0 0.009261403102570039 0.0 15 0.0 0.0 0.0 0.009261403102570039 0.0 16 0.0 0.0 0.0 0.009261403102570039 0.0 17 0.0 0.0 0.0 0.0231535077564251 0.0 18 0.0 0.0 0.0 0.0463070155128502 0.0 19 0.0 0.0 0.0 0.05556841861542024 0.0 20 0.0 0.0 0.0 0.060199120166705256 0.0 21 0.0 0.0 0.0 0.06946052326927529 0.0 22 0.0 0.0 0.0 0.07409122482056031 0.0 23 0.0 0.0 0.0 0.07872192637184533 0.0 24 0.0 0.0 0.0 0.08335262792313035 0.0 25 0.0 0.0 0.0 0.08798332947441537 0.0 26 0.0 0.0 0.0 0.10187543412827044 0.0 27 0.0 0.0 0.0 0.13429034498726558 0.0 28 0.0 0.0 0.0 0.14818244964112062 0.0 29 0.0 0.0 0.0 0.14818244964112062 0.0 30 0.0 0.0 0.0 0.15744385274369066 0.0 31 0.0 0.0 0.0 0.17596665894883073 0.0 32 0.0 0.0 0.0 0.19448946515397084 0.0 33 0.0 0.0 0.0 0.23153507756425099 0.0 34 0.0 0.0 0.0 0.31951840703866635 0.0 35 0.0 0.0 0.0 0.38434822875665664 0.0 36 0.0 0.0 0.0 0.5093771706413521 0.0 37 0.0 0.0 0.0 0.6899745311414679 0.0 38 0.0 0.0 0.0 0.8844639962954387 0.0 39 0.0 0.0 0.0 1.0974762676545498 0.0 40 0.0 0.0 0.0 1.2965964343598055 0.0 41 0.0 0.0 0.0 1.5929613336420467 0.0 42 0.0 0.0 0.0 1.884695531373003 0.0 43 0.0 0.0 0.0 2.1903218337578143 0.0 44 0.0 0.0 0.0 2.4913174345913407 0.0 45 0.0 0.0 0.0 2.7506367214633016 0.0 46 0.0 0.0 0.0 3.084047233155823 0.0 47 0.0 0.0 0.0 3.347997221579069 0.0 48 0.0 0.0 0.0 3.60731650845103 0.0 49 0.0 0.0 0.0 3.8944200046307014 0.0 50 0.0 0.0 0.0 4.172262097707803 0.0 51 0.0 0.0 0.0 4.519564714054179 0.0 52 0.0 0.0 0.0 4.8159296133364204 0.0 53 0.0 0.0 0.0 5.140078721926372 0.0 54 0.0 0.0 0.0 5.357721694836767 0.0 55 0.0 0.0 0.0 5.552211159990739 0.0 56 0.0 0.0 0.0 5.746700625144709 0.0 57 0.0 0.0 0.0 5.95971289650382 0.0 58 0.0 0.0 0.0 6.107895346144941 0.0 59 0.0 0.0 0.0 6.330169020606622 0.0 60 0.0 0.0 0.0 6.501504978004168 0.0 61 0.0 0.0 0.0 6.7191479509145635 0.0 62 0.0 0.0 0.0 6.858068997453114 0.0 63 0.0 0.0 0.0 7.03866635795323 0.0 64 0.0 0.0 0.0 7.284093540171336 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACA 25 2.7474834E-6 70.00001 3 TTATAAC 25 2.7474834E-6 70.00001 2 TTATTAC 20 7.7536926E-5 70.0 2 ATTACAC 20 7.7536926E-5 70.0 4 TCTCGTC 15 0.0021953168 70.0 38 CTTATTA 20 7.7536926E-5 70.0 1 CGAGCAC 15 0.0021953168 70.0 15 CCGAGCA 15 0.0021953168 70.0 14 TATTACA 20 7.7536926E-5 70.0 3 GTGGTGC 15 0.0021953168 70.0 28 TCTTTAT 35 3.4888217E-9 70.0 1 GCACACG 15 0.0021953168 70.0 18 TTTACAC 130 0.0 67.30769 2 CTTTACA 125 0.0 67.2 1 CTATACA 60 0.0 64.166664 1 CTTAATA 35 2.8402792E-7 60.0 1 AATACAC 35 2.8402792E-7 60.0 4 TTAATAC 35 2.8402792E-7 60.0 2 ATAACAC 30 8.105602E-6 58.333332 4 CTTATAA 30 8.105602E-6 58.333332 1 >>END_MODULE