FastQCFastQC Report
Wed 25 May 2016
SRR1780411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122309
Sequences flagged as poor quality0
Sequence length50
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA1250.036.967911
AATGGAT250.002323309435.265321
GCTAGCA250.002337379635.2219634
ACTTCTG250.0023609835.149968
GTACGCC250.0023609835.149963
TTACACA1450.034.8469433
TTTACAC1450.033.3318562
GTAGAAC353.1893843E-431.44817735
ATGTATT353.2201866E-431.39672928
ATGCGAC300.005684802329.37569814
AGCACTT300.005696211529.3636612
CTGATAC300.00571908429.3396131
CCGGGTT300.00575352829.30361427
CTGGATC300.00575352829.30361443
TAGAACT406.970273E-427.51715536
AGAACTC406.970273E-427.51715537
CTATACA508.2692495E-526.4056511
TTATACA5200.024.5035782
TATACAC5400.024.4096953
TACGCCC551.5975698E-423.965884
Produced by FastQC (version 0.11.5)