FastQCFastQC Report
Wed 25 May 2016
SRR1780411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122309
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT2716222.207687087622332No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT2153917.61031485826881TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC75866.2023236229549745No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC37463.062734549379032No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG27852.2770196796638023No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT20141.6466490609848825No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC15291.250112420181671No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC11660.9533231405701951No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9850.8053373014250791No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8350.6826971032385188No Hit
CTTAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC7610.6221946054664824No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC6050.4946487993524598No Hit
CTTATTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4650.38018461437833684No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC4290.3507509668135624No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3950.3229525218912754No Hit
CTTATCACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC3470.2837076584715761No Hit
CTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT3170.25917961883426405No Hit
CTTATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC3010.24609799769436425No Hit
ACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTTCT2350.19213631049227775RNA PCR Primer, Index 18 (95% over 23bp)
TCTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT1920.15697945367879715No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGC1700.13899222461143496No Hit
TCTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT1680.1373570219689475No Hit
ATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTTCTGCTT1550.12672820479277896TruSeq Adapter, Index 18 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCTC207.829244E-443.999597
CCGTCTT22950.042.84927744
CTTTACA8300.041.365931
TTTACAC8350.041.1014142
CTTATAA950.039.3841551
GCCGTCT25350.038.6189743
TTAACAC800.038.499642
CTATACA4250.037.8033371
TCTTTAT1000.037.414951
ATAACAC1000.037.3996544
CTTATAC34100.035.5625951
ATTACAC751.8189894E-1235.1996734
CACAATT250.002344666735.19967320
TTACACA9750.035.1996733
ATACCTT250.002344666735.19967344
TTATACA35350.034.2910542
CTTAACA900.034.23591
TATAACA1100.033.9996833
TCTTTAC607.854396E-933.0131871
TCTTAAT1200.031.1791231
Produced by FastQC (version 0.11.5)