FastQCFastQC Report
Wed 25 May 2016
SRR1780410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44811
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAGG200.006910903452.49581518
TGAACTA200.006910903452.49581568
CACAGGT200.006910903452.49581519
GTATCAT200.006910903452.49581554
TCATCGA200.006910903452.49581545
CATCGAA200.006910903452.49581546
GACGAAT305.821065E-446.66294523
TTATCCA404.52627E-543.7465132
GAACATA900.005202064319.44289669
CTTATCC950.006882435618.522951
CCCAACA1300.002163491216.24321
AACATAA1350.002889442515.55431570
CCGACGA2659.13511E-613.20649421
GCCGACG2751.3380792E-512.72625820
CCCAACC2852.518742E-411.113771
CCCCACC2600.001474595910.82881
CGCTGCC3300.00102476329.54469316
TGCCGAC2950.0044079479.49076819
GCTGCCG3050.0058017829.17959617
CTGCCGA3050.0058017829.17959618
Produced by FastQC (version 0.11.5)