##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780410_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44811 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.757782687286603 32.0 21.0 34.0 12.0 34.0 2 27.169601213987637 32.0 21.0 34.0 11.0 34.0 3 27.521144361875432 33.0 23.0 34.0 11.0 34.0 4 27.952043025150076 34.0 23.0 34.0 11.0 34.0 5 27.981879449242374 34.0 23.0 34.0 11.0 34.0 6 30.072794626319432 35.0 24.0 38.0 11.0 38.0 7 29.347905648166744 34.0 23.0 37.0 11.0 38.0 8 28.390618374952577 34.0 21.0 37.0 11.0 38.0 9 29.358996674923567 34.0 21.0 38.0 12.0 38.0 10-11 29.23189618620428 34.0 21.5 38.0 11.0 38.0 12-13 29.522606056548614 35.5 21.5 38.0 11.0 38.0 14-15 29.151413715382382 34.5 21.0 38.0 11.0 38.0 16-17 28.920968065876682 34.0 21.0 38.0 11.0 38.0 18-19 28.98273861328692 34.5 21.0 38.0 11.0 38.0 20-21 29.156769543192517 34.0 21.5 38.0 11.0 38.0 22-23 28.813572560308856 34.0 21.0 38.0 11.0 38.0 24-25 29.178929280756957 35.5 21.0 38.0 11.0 38.0 26-27 29.133873379304188 36.0 21.0 38.0 11.0 38.0 28-29 29.05287764165049 36.0 21.0 38.0 11.0 38.0 30-31 28.910791993037424 36.0 21.0 38.0 11.0 38.0 32-33 28.882417263618308 35.0 21.0 38.0 11.0 38.0 34-35 28.983698199102896 34.5 21.0 38.0 11.0 38.0 36-37 28.79578674878936 34.5 21.0 38.0 11.0 38.0 38-39 28.999988842025395 36.0 21.0 38.0 11.0 38.0 40-41 28.772165316551742 34.5 21.0 38.0 11.0 38.0 42-43 29.06100064716253 35.0 21.0 38.0 11.0 38.0 44-45 28.33184932271094 34.0 21.0 38.0 11.0 38.0 46-47 28.964930485818215 34.5 21.0 38.0 11.0 38.0 48-49 28.908917453303875 34.0 21.0 38.0 11.0 38.0 50-51 28.875398897592106 34.0 21.0 38.0 11.0 38.0 52-53 28.751578853406528 34.0 21.0 38.0 11.0 38.0 54-55 28.61155743009529 34.0 21.0 38.0 11.0 38.0 56-57 28.464227533418132 34.0 21.0 38.0 11.0 38.0 58-59 28.308562629711453 34.0 20.5 38.0 11.0 38.0 60-61 28.534946776461137 34.0 21.0 38.0 11.0 38.0 62-63 28.42141438486086 34.0 20.0 38.0 11.0 38.0 64-65 28.51095713106157 34.0 20.5 38.0 11.0 38.0 66-67 28.65784070875455 34.0 21.0 38.0 11.0 38.0 68-69 28.395193144540404 34.0 20.0 38.0 11.0 38.0 70-71 28.38977036888264 34.0 20.0 37.5 11.0 38.0 72-73 28.37943808439892 34.0 19.5 37.5 11.0 38.0 74-75 27.84540626185535 33.0 19.0 37.0 11.0 38.0 76 26.925710205083572 31.0 11.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.3424713816899825 1101 2 -0.842624553440249 1101 3 -2.0495505689066498 1101 4 -1.811789486412895 1101 5 -2.8788338614853473 1101 6 -3.426164537720215 1101 7 -2.492177505089032 1101 8 -1.2790280818583426 1101 9 -2.653337254937334 1101 10-11 -1.8842354457149675 1101 12-13 -2.249299989929643 1101 14-15 -1.355001913247925 1101 16-17 -1.770130117440182 1101 18-19 -1.5189702007094574 1101 20-21 -1.2915923952701576 1101 22-23 -0.7988561792948623 1101 24-25 -2.4876906137856167 1101 26-27 -1.4970625276508365 1101 28-29 -1.7385507866589123 1101 30-31 -1.7208174526373945 1101 32-33 -1.387989990737836 1101 34-35 -1.8340392487729567 1101 36-37 -1.3760817715714282 1101 38-39 -2.2703164177218333 1101 40-41 -2.0345771430483985 1101 42-43 -1.7705734283105272 1101 44-45 -1.542616580688577 1101 46-47 -2.0084206982605757 1101 48-49 -2.6615263056715754 1101 50-51 -1.5049265290734546 1101 52-53 -2.4045042217316634 1101 54-55 -2.7874053377074013 1101 56-57 -2.376263968376694 1101 58-59 -1.7666099668701314 1101 60-61 -1.6544678281072827 1101 62-63 -2.7356127801593964 1101 64-65 -2.2230964436359884 1101 66-67 -2.8144461022607317 1101 68-69 -1.6888819828969943 1101 70-71 -1.9533843756621927 1101 72-73 -3.146715795851609 1101 74-75 -2.434159896740862 1101 76 -1.8762309605481313 1102 1 0.11141776595789565 1102 2 -0.335948571449876 1102 3 0.795970312943993 1102 4 1.6580909669328605 1102 5 0.9970394501122328 1102 6 0.7733386450153823 1102 7 1.486397250383721 1102 8 -0.35650052402570864 1102 9 -0.35617842866811955 1102 10-11 -1.3236082139186607 1102 12-13 -3.026392056378949 1102 14-15 -1.3348963395792381 1102 16-17 -2.162538128618568 1102 18-19 -0.6207012983651019 1102 20-21 -0.6959162580243898 1102 22-23 -1.4045462895261949 1102 24-25 -1.1018033292024754 1102 26-27 -4.5011767956216495 1102 28-29 -0.900404536076703 1102 30-31 -0.152932031118997 1102 32-33 -4.396140898981898 1102 34-35 -1.0850095949924885 1102 36-37 -1.125150239200675 1102 38-39 -0.5564132970883904 1102 40-41 -0.3386060205518895 1102 42-43 -0.17637221731844122 1102 44-45 -4.421439744793528 1102 46-47 -0.8232398257252527 1102 48-49 -1.217107737126316 1102 50-51 -4.683703116559872 1102 52-53 -0.6592395112829728 1102 54-55 0.1705204502137292 1102 56-57 -1.167251392689689 1102 58-59 -0.036405029748568296 1102 60-61 -0.16079448545862718 1102 62-63 -3.792669137867829 1102 64-65 -0.19045399681095887 1102 66-67 -0.0024215719083002796 1102 68-69 -5.745899526756645 1102 70-71 -1.1139960819189838 1102 72-73 -0.6009930819873048 1102 74-75 -5.4893143758590135 1102 76 -2.549030215322233 1103 1 -0.35635451471940627 1103 2 -0.3804750852816632 1103 3 0.13887324653747157 1103 4 1.0366011752807083 1103 5 -0.5454314578384327 1103 6 -0.8991580451107382 1103 7 -0.5120697553998461 1103 8 2.0344130011641752 1103 9 1.657479156169174 1103 10-11 0.36724956602698455 1103 12-13 -0.4969515992679483 1103 14-15 0.4321233803009079 1103 16-17 0.07197514571771535 1103 18-19 0.3467225745357432 1103 20-21 -0.26835023475075204 1103 22-23 -0.3952438018946687 1103 24-25 1.139329692886573 1103 26-27 -0.7211266249018387 1103 28-29 0.29108037786657803 1103 30-31 0.5805708606655493 1103 32-33 -1.7582759418360538 1103 34-35 -0.7460436692730283 1103 36-37 -0.42819532316694975 1103 38-39 0.8420955176283549 1103 40-41 0.714386802247919 1103 42-43 0.5216976035999608 1103 44-45 -2.621425808482467 1103 46-47 -0.22913488530297954 1103 48-49 1.217600645564783 1103 50-51 -0.2086839541321659 1103 52-53 0.23885121410201648 1103 54-55 0.7583192098820462 1103 56-57 0.06437009710196406 1103 58-59 0.45251560295995574 1103 60-61 0.9974523266102189 1103 62-63 0.9401020975861449 1103 64-65 0.6355511596241321 1103 66-67 0.5685142513792734 1103 68-69 -2.4939310228196376 1103 70-71 -0.26882849777850737 1103 72-73 1.2929167500801455 1103 74-75 -0.20564076426400035 1103 76 1.3233131753822462 1104 1 -0.29348352743897266 1104 2 0.34914332780131474 1104 3 0.36812824350900186 1104 4 0.06095005474769977 1104 5 0.2320973126037238 1104 6 -0.631562648381486 1104 7 0.4365674341822263 1104 8 0.6292040369000915 1104 9 0.3523307612570008 1104 10-11 0.5956565920718084 1104 12-13 -0.4378964811577113 1104 14-15 0.5076836468060542 1104 16-17 1.0335136072561752 1104 18-19 0.48818229591672235 1104 20-21 -0.24530359634978183 1104 22-23 0.505664738383949 1104 24-25 0.7570462283196484 1104 26-27 1.1678497530509162 1104 28-29 0.4624613590900779 1104 30-31 -0.01879316113941698 1104 32-33 -0.043860436081967435 1104 34-35 1.0717637201879064 1104 36-37 1.7725012243799902 1104 38-39 0.6470924891607588 1104 40-41 1.0976090917694172 1104 42-43 0.3654589603534433 1104 44-45 -2.1394451906750795 1104 46-47 0.9969099360174276 1104 48-49 1.0422523717913137 1104 50-51 0.977597087660687 1104 52-53 0.28442965141273646 1104 54-55 0.790057550281805 1104 56-57 0.3783919020686497 1104 58-59 0.8086936768545634 1104 60-61 0.7565982987483153 1104 62-63 1.2040633332009243 1104 64-65 0.8383070651359432 1104 66-67 0.7460127371454774 1104 68-69 -1.8305149113720276 1104 70-71 0.14189227750919997 1104 72-73 0.6866175374817232 1104 74-75 0.22521932053308902 1104 76 1.2193155981563244 1105 1 0.01921772479716566 1105 2 -0.32956865788741396 1105 3 -0.9267435513627902 1105 4 -1.3113814896768687 1105 5 0.18624161791081661 1105 6 -0.5204117837422473 1105 7 0.4249991574976697 1105 8 -0.7106886285739691 1105 9 -1.3020520652188488 1105 10-11 -0.7002289177671912 1105 12-13 -1.713090279607325 1105 14-15 -1.5377844509876226 1105 16-17 -0.5714357069954659 1105 18-19 -0.031108103606232618 1105 20-21 -1.3122982296234866 1105 22-23 -0.914013258038235 1105 24-25 -0.6859770274943067 1105 26-27 -1.0476541229180754 1105 28-29 -1.4800848543863694 1105 30-31 -0.6709398516582006 1105 32-33 -0.22275131264726156 1105 34-35 -0.20307229650857295 1105 36-37 -0.5181666348925198 1105 38-39 -1.0072605221689948 1105 40-41 -1.024275225463736 1105 42-43 -0.9424420533615248 1105 44-45 -0.09994012210024295 1105 46-47 -0.6664174342866858 1105 48-49 -1.3575687373321195 1105 50-51 -0.7566605152015669 1105 52-53 -1.402362238867525 1105 54-55 -2.0523992177563635 1105 56-57 -0.8590261062795932 1105 58-59 -0.6789926678085259 1105 60-61 -0.9046718264752975 1105 62-63 -1.2107249792618084 1105 64-65 -1.1868867333136706 1105 66-67 -1.2902722956511852 1105 68-69 -0.6102406120279618 1105 70-71 -1.1763545919004628 1105 72-73 -1.3017545555415353 1105 74-75 -2.3257347641419237 1105 76 -1.3460392020821992 1106 1 0.23762015149079474 1106 2 0.7377720903431211 1106 3 -0.3412363049859799 1106 4 0.5873714594299742 1106 5 0.6692210584231191 1106 6 0.0038888566352390797 1106 7 0.3201794743160029 1106 8 0.6579665787061124 1106 9 1.4198892896850914 1106 10-11 0.2779308395634459 1106 12-13 -0.029200828983800164 1106 14-15 0.8556769578428458 1106 16-17 0.6676273196307605 1106 18-19 0.4288177474217427 1106 20-21 -0.8769089475203486 1106 22-23 0.5484741029324418 1106 24-25 -0.014692902115132966 1106 26-27 0.10263236174656853 1106 28-29 0.4248359410298761 1106 30-31 -0.7005657363902529 1106 32-33 -0.26258953307193167 1106 34-35 0.16474030881666835 1106 36-37 0.003437679229488566 1106 38-39 -0.06745600582251754 1106 40-41 0.17720775397677713 1106 42-43 0.7325158165407331 1106 44-45 0.7470096588232487 1106 46-47 -0.27733755562136864 1106 48-49 -0.0286506382421301 1106 50-51 0.4403061177610219 1106 52-53 -0.11941650243341684 1106 54-55 0.20176323590053258 1106 56-57 1.1487932398612912 1106 58-59 -0.3928113398678441 1106 60-61 0.6517488004205596 1106 62-63 0.5161034669801872 1106 64-65 0.010046195570723171 1106 66-67 0.6354776200886221 1106 68-69 0.8062743862868302 1106 70-71 1.1913906052683956 1106 72-73 -0.2177302886052317 1106 74-75 -0.25387766943346435 1106 76 -0.4412195189005317 1107 1 0.6990408495924463 1107 2 0.40298948164747017 1107 3 -0.24833979549424612 1107 4 -1.0279990243422255 1107 5 -0.5637856776454662 1107 6 0.2130744047062727 1107 7 -1.5007023382988152 1107 8 -0.32041676483338577 1107 9 -0.7463691304006375 1107 10-11 -0.23863376545798687 1107 12-13 -0.5752204248196797 1107 14-15 0.10194150162729088 1107 16-17 -0.4421093613245404 1107 18-19 0.07875651043459797 1107 20-21 0.2455956452536867 1107 22-23 -0.7150286527319771 1107 24-25 -1.230277715342325 1107 26-27 -0.697643204695563 1107 28-29 -1.5159785108990889 1107 30-31 -0.3249686757656356 1107 32-33 0.3922175704185733 1107 34-35 -0.9779112527265035 1107 36-37 -0.29120408439574064 1107 38-39 -0.19841130282190633 1107 40-41 -0.17227173163253084 1107 42-43 -0.4786905521059275 1107 44-45 0.44749955472036973 1107 46-47 -1.2401973229208174 1107 48-49 -0.02871187522927343 1107 50-51 -0.40176369240431953 1107 52-53 0.046076455313063036 1107 54-55 0.05852992297952753 1107 56-57 -0.536938542135033 1107 58-59 -0.3466692700576779 1107 60-61 -1.0170203361379215 1107 62-63 -1.0840190069940903 1107 64-65 -0.10887603345560848 1107 66-67 -0.4429363419444492 1107 68-69 0.4759314591588506 1107 70-71 -0.28313480808126457 1107 72-73 -0.22535429350418923 1107 74-75 -1.0256474183618174 1107 76 -0.3358306640321942 1108 1 -0.23935247330791753 1108 2 -1.3340475553895672 1108 3 -0.8378546624739016 1108 4 -1.3527251589275124 1108 5 -1.156221775715366 1108 6 -1.7626167794356142 1108 7 -0.06371746610267692 1108 8 -0.1246421169460632 1108 9 0.6714190803453199 1108 10-11 -0.3202978238826084 1108 12-13 -1.2823409256021527 1108 14-15 -0.36767247855006957 1108 16-17 -1.1243211505785808 1108 18-19 -2.1603932026587778 1108 20-21 -0.717810718856903 1108 22-23 -1.337355204635994 1108 24-25 -1.1744352531618354 1108 26-27 -1.1395576543564907 1108 28-29 -1.5601882135594956 1108 30-31 -1.0140923064385596 1108 32-33 -0.9150855073070403 1108 34-35 -1.0299456815214967 1108 36-37 -1.301715299836534 1108 38-39 -1.096924604856337 1108 40-41 -1.5459806518203258 1108 42-43 -1.9301250852305998 1108 44-45 -1.3244879257178148 1108 46-47 -0.42645188734439543 1108 48-49 -0.254898625359683 1108 50-51 -1.3402091801455818 1108 52-53 -2.2083053913223054 1108 54-55 -1.5367230764988271 1108 56-57 -0.9798702061934605 1108 58-59 -1.9612493430884577 1108 60-61 -1.1914073992573826 1108 62-63 -1.0141702850326944 1108 64-65 -1.9042855274566506 1108 66-67 -0.9917365506038109 1108 68-69 -0.7125661934233136 1108 70-71 -0.026483790866869583 1108 72-73 -1.0170861662219792 1108 74-75 -0.6535556082418381 1108 76 -0.6869522081114852 1109 1 0.007152678460393247 1109 2 -0.029341345420199616 1109 3 0.2787702330983599 1109 4 -0.607528834783821 1109 5 -0.3487586735154231 1109 6 0.03749461014945865 1109 7 -0.20646321937474355 1109 8 0.452801492076496 1109 9 -0.31499139027567935 1109 10-11 0.6069926244604709 1109 12-13 -0.26120475183440206 1109 14-15 -0.6946009107416629 1109 16-17 0.41032101037936997 1109 18-19 0.18341075167149157 1109 20-21 0.5699364267849774 1109 22-23 -0.5400842494703006 1109 24-25 0.24163269448505886 1109 26-27 0.6107068959080593 1109 28-29 0.3328777847696607 1109 30-31 0.37504720901674204 1109 32-33 0.5706566258035153 1109 34-35 0.6045572424551153 1109 36-37 0.6897077021127807 1109 38-39 1.193969296563882 1109 40-41 1.0387311276282958 1109 42-43 0.5282987521406035 1109 44-45 0.08770923384776452 1109 46-47 -0.3097991443758623 1109 48-49 -1.113907258052528 1109 50-51 -0.18287139122884355 1109 52-53 0.27907973816808607 1109 54-55 -0.5969291472312115 1109 56-57 -0.03603840446692175 1109 58-59 -0.6143543277723857 1109 60-61 0.6995421969553739 1109 62-63 -0.6303496222646601 1109 64-65 -0.0921440626836052 1109 66-67 0.6485864791929004 1109 68-69 1.4683861875290702 1109 70-71 0.12430985992678245 1109 72-73 -0.5126305828190283 1109 74-75 0.08025113082805646 1109 76 -0.18826103573610098 1110 1 0.005151708293038126 1110 2 0.6207314171313421 1110 3 0.30175107229312204 1110 4 0.6239773500330585 1110 5 0.5426432183109178 1110 6 0.6751371034795568 1110 7 0.19500415874798094 1110 8 0.9592288507958742 1110 9 -0.44196204271322515 1110 10-11 -0.1947900580522628 1110 12-13 0.11049061561648088 1110 14-15 0.27629406864228656 1110 16-17 0.6082654682983595 1110 18-19 0.5451922214750375 1110 20-21 0.6470040959579109 1110 22-23 0.8699327284583909 1110 24-25 0.7094655831583587 1110 26-27 -0.6611825050135991 1110 28-29 -0.12649645728709658 1110 30-31 -0.07735395518408339 1110 32-33 1.2306432860023975 1110 34-35 0.03367439016309248 1110 36-37 0.5428486189209316 1110 38-39 0.9497599829572856 1110 40-41 1.3327827890398893 1110 42-43 1.3427542457132446 1110 44-45 1.7764977373646218 1110 46-47 1.9008181246030382 1110 48-49 0.9417560938756786 1110 50-51 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44-45 1.5144009631710738 2116 46-47 0.03935499096248307 2116 48-49 0.7664968772858209 2116 50-51 1.3530778568914563 2116 52-53 0.2734406404237255 2116 54-55 1.2660877700620219 2116 56-57 0.3044221218488694 2116 58-59 0.0014821383930261334 2116 60-61 0.23455159545161308 2116 62-63 1.542112783750376 2116 64-65 0.9766999222788009 2116 66-67 1.0205319679147067 2116 68-69 0.37463618752907024 2116 70-71 -1.4694401400732175 2116 72-73 0.15090325176743846 2116 74-75 -0.9740251849614161 2116 76 -0.2159865996458734 2117 1 0.4857019929230191 2117 2 0.5500483051768832 2117 3 1.6909035074607388 2117 4 -0.2220062046791469 2117 5 -1.0191847386783301 2117 6 0.10840718570599606 2117 7 -0.3064189911571411 2117 8 0.2204559222696254 2117 9 1.2481075335043599 2117 10-11 0.7795932436555191 2117 12-13 1.9689662639403274 2117 14-15 1.2946368896416516 2117 16-17 1.5523673025804605 2117 18-19 1.3638987363390456 2117 20-21 0.27935703713438187 2117 22-23 0.908832159198429 2117 24-25 1.4854776008686663 2117 26-27 1.6541242628548396 2117 28-29 1.3744523093163217 2117 30-31 0.6914632491169961 2117 32-33 1.8614940133901285 2117 34-35 0.6236490897203382 2117 36-37 -0.22154100789753883 2117 38-39 0.9900034997188278 2117 40-41 0.22264679916006713 2117 42-43 -0.25354556453343235 2117 44-45 1.4261656690534288 2117 46-47 1.6494740385815305 2117 48-49 0.937802899694784 2117 50-51 1.088984719636553 2117 52-53 2.890387419135209 2117 54-55 -0.45458800024610113 2117 56-57 0.8631581162466304 2117 58-59 0.4408203736871421 2117 60-61 1.5163268055356447 2117 62-63 0.5303830918736274 2117 64-65 0.03438549650849154 2117 66-67 -0.7349827379676483 2117 68-69 1.1168455712825676 2117 70-71 1.5927972548847649 2117 72-73 2.6032842041483875 2117 74-75 1.4453550671394275 2117 76 1.1598887504941828 2118 1 0.32175863780319247 2118 2 1.2363228149808023 2118 3 0.34177496715572886 2118 4 -0.611984418186772 2118 5 -0.40807362756721943 2118 6 0.418864702045866 2118 7 1.5955417931565847 2118 8 -2.043160635464581 2118 9 -1.0008031418769043 2118 10-11 -0.31844597203075864 2118 12-13 0.6454368521756244 2118 14-15 -0.3769317378093291 2118 16-17 0.33586403460660463 2118 18-19 -0.26409690636247873 2118 20-21 -1.5520699781161618 2118 22-23 0.7228299805491893 2118 24-25 0.3931573394307577 2118 26-27 0.8150719752731383 2118 28-29 -0.027780806152087223 2118 30-31 0.0016484343021794245 2118 32-33 -0.828048470270005 2118 34-35 0.4932024666266521 2118 36-37 0.4723587742375379 2118 38-39 0.2114087284770001 2118 40-41 -0.674684355524029 2118 42-43 0.5291341740286555 2118 44-45 0.2458824446525547 2118 46-47 -0.0866370725295802 2118 48-49 0.1376939672329094 2118 50-51 -0.08557955051595201 2118 52-53 0.07039831238139982 2118 54-55 0.7030917383159903 2118 56-57 0.9127223863991354 2118 58-59 1.2989358420967285 2118 60-61 -0.5414073992573805 2118 62-63 0.6469408260784171 2118 64-65 0.7225663243952027 2118 66-67 -0.8926624765297362 2118 68-69 1.6800263991692788 2118 70-71 0.14018287579979472 2118 72-73 0.07087679674098268 2118 74-75 1.2473703176840907 2118 76 -1.1554707266300035 2119 1 0.3993049340994901 2119 2 1.819656148314138 2119 3 0.781589781970542 2119 4 0.7792192855169304 2119 5 0.8453985946550056 2119 6 1.6445591464903089 2119 7 -0.8732082068434153 2119 8 1.548274549720606 2119 9 -0.11770129002505314 2119 10-11 1.0200956946359128 2119 12-13 0.5985618521756209 2119 14-15 1.2584849288573352 2119 16-17 1.1084334790510475 2119 18-19 0.8724771677115939 2119 20-21 1.6574207626245787 2119 22-23 2.2118346101788227 2119 24-25 0.4903795616529827 2119 26-27 1.0199330863842526 2119 28-29 1.5196728975516152 2119 30-31 0.6289632491169961 2119 32-33 1.0278543075077735 2119 34-35 1.3901932073673926 2119 36-37 -0.03342826279949662 2119 38-39 0.8659226173658858 2119 40-41 0.6279776815130056 2119 42-43 0.30864806291754476 2119 44-45 0.727942629837738 2119 46-47 1.9515573719148627 2119 48-49 0.19730044871439034 2119 50-51 1.0614111902247885 2119 52-53 0.38058349756658316 2119 54-55 -0.23093603946178476 2119 56-57 1.8542733123250628 2119 58-59 1.3608571383930261 2119 60-61 0.11773611926113858 2119 62-63 2.0138389742265694 2119 64-65 1.3070570651359432 2119 66-67 0.827823634581371 2119 68-69 0.8791004732433549 2119 70-71 0.888446764688684 2119 72-73 -0.6362991291849447 2119 74-75 0.8607962436100145 2119 76 1.2438348289255536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 9.0 13 98.0 14 437.0 15 1052.0 16 1827.0 17 2334.0 18 2606.0 19 2494.0 20 1961.0 21 1560.0 22 1174.0 23 809.0 24 689.0 25 611.0 26 598.0 27 606.0 28 662.0 29 824.0 30 934.0 31 1153.0 32 1527.0 33 1833.0 34 2604.0 35 3763.0 36 6081.0 37 6563.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.792131928374037 23.161607764721744 9.757577119234313 39.28868318766991 2 13.884983597777332 21.563901720559684 29.885519180558344 34.66559550110464 3 12.579500569056703 24.03427729798487 26.245787864586823 37.1404342683716 4 9.120528441677267 30.13099462185624 30.22249001361273 30.525986922853765 5 9.118296846756376 43.4313003503604 27.640534690143042 19.809868112740176 6 17.658610609002253 35.424337774207224 14.947222780120953 31.969828836669567 7 17.451072281359487 31.19769699404164 17.596125951217335 33.75510477338153 8 16.16121041708509 34.437972819173865 21.992367945370557 27.40844881837049 9 18.319162705585683 21.92765169266475 19.075673383767377 40.67751221798219 10-11 17.769074557586308 26.996719555466292 26.244672067126373 28.989533819821027 12-13 17.59054696391511 24.539733547566446 25.645488830867418 32.22423065765102 14-15 18.156256276360715 26.937582290062707 22.498939992412577 32.407221441164005 16-17 20.730401017607285 28.25310749592734 22.821405458481177 28.1950860279842 18-19 18.69295485483475 26.761286291312402 23.374840998861888 31.17091785499096 20-21 17.202249447680256 27.427417375198054 23.513199883957064 31.857133293164626 22-23 21.736850326928657 27.20202628818817 22.628372497824195 28.432750887058983 24-25 19.191716319653658 30.37200687331236 22.32264399366227 28.11363281337172 26-27 17.105175068621545 31.16310727276785 24.642386913927382 27.089330744683227 28-29 19.93372163084957 27.91055767557073 26.808149784651093 25.347570908928613 30-31 19.36466492602263 27.487670438062082 27.342616768204238 25.80504786771105 32-33 16.725803932070253 31.221128740710984 26.272567003637498 25.78050032358126 34-35 18.05137131507889 29.81968713039209 25.100979670170272 27.027961884358753 36-37 18.50661667894044 27.562428867911898 26.397536319207337 27.53341813394033 38-39 18.21985673160608 26.11412376425431 28.31112896387048 27.35489054026913 40-41 17.062774765124637 26.013701992814266 27.97527392827654 28.94824931378456 42-43 15.28977260047756 25.90100644930932 29.286335944299392 29.522885005913725 44-45 15.592153712258149 26.83716051862266 29.8844033830979 27.68628238602129 46-47 15.990493405637007 28.446140456584324 27.793398942224005 27.769967195554663 48-49 15.895650621499186 29.7281917386356 25.377697440360624 28.998460199504585 50-51 15.65686996496396 28.488540760081232 25.183548682243202 30.671040592711613 52-53 15.66133315480574 27.97973711811832 26.443284015085585 29.915645711990358 54-55 15.269688246189553 28.343487090223384 25.23933855526545 31.147486108321615 56-57 16.90098413336011 28.02660061145701 24.612260382495368 30.46015487268751 58-59 16.55731851554306 27.550155095847 24.82537769744036 31.067148691169578 60-61 15.441521055098079 26.740086139563946 24.911294101894622 32.90709870344335 62-63 16.24154783423713 27.585860614581243 24.326616232621454 31.84597531856018 64-65 17.290397447055412 27.89382071366406 22.483318827966347 32.33246301131419 66-67 15.72604940751155 31.147486108321615 21.579522885005915 31.54694159916092 68-69 15.632322420834171 31.47776215661333 21.432237620227177 31.457677802325325 70-71 15.602195889402157 32.51656959228761 21.42889022784584 30.452344290464396 72-73 15.362299435406484 32.584633237374746 21.499185467853877 30.55388185936489 74-75 17.096248688937983 32.369284327508865 21.60295463167526 28.931512351877885 76 17.761263975363192 31.079422463234472 21.87409341456339 29.28522014683895 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.5 14 4.0 15 6.0 16 7.5 17 10.0 18 13.0 19 13.5 20 13.0 21 15.0 22 22.0 23 38.0 24 52.0 25 57.0 26 84.5 27 125.5 28 173.5 29 208.0 30 235.5 31 325.5 32 463.0 33 538.0 34 640.5 35 840.0 36 1031.0 37 1125.0 38 1326.5 39 1746.0 40 2172.0 41 2516.0 42 2652.0 43 2772.0 44 3029.0 45 3176.0 46 3186.0 47 3238.5 48 3177.0 49 2911.5 50 2760.0 51 2557.0 52 2237.5 53 2005.0 54 1889.0 55 1764.0 56 1460.5 57 1127.0 58 972.0 59 830.0 60 635.0 61 502.5 62 423.0 63 372.5 64 284.0 65 218.0 66 160.5 67 131.0 68 120.5 69 94.0 70 64.5 71 51.0 72 48.0 73 34.5 74 22.5 75 21.0 76 20.5 77 16.0 78 9.0 79 6.0 80 6.5 81 6.0 82 8.0 83 11.0 84 9.0 85 6.5 86 5.5 87 5.0 88 4.5 89 3.5 90 3.0 91 2.5 92 2.0 93 3.5 94 3.5 95 2.5 96 3.0 97 1.5 98 0.5 99 3.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6739416661087679 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 44811.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.42964897011895 #Duplication Level Percentage of deduplicated Percentage of total 1 86.22935700492837 66.76708843810671 2 7.0207798945153765 10.872330454575884 3 3.0579012594748827 7.1031666331927426 4 1.7177277574430068 5.320122291401665 5 0.7868115399025852 3.0461270670147953 6 0.48130962331037264 2.23605811073174 7 0.28820935527567226 1.562116444622972 8 0.18733608092918697 1.1604293588627792 9 0.10951955500475546 0.7632054629443663 >10 0.12104792921578235 1.1693557385463391 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00223159492088996 0.0 2 0.0 0.0 0.0 0.00223159492088996 0.0 3 0.0 0.0 0.0 0.00669478476266988 0.0 4 0.0 0.0 0.0 0.00892637968355984 0.0 5 0.0 0.0 0.0 0.01562116444622972 0.0 6 0.0 0.0 0.0 0.01562116444622972 0.0 7 0.0 0.0 0.0 0.01562116444622972 0.0 8 0.0 0.0 0.0 0.01562116444622972 0.0 9 0.0 0.0 0.0 0.02008435428800964 0.0 10 0.0 0.0 0.0 0.02008435428800964 0.0 11 0.0 0.0 0.0 0.02008435428800964 0.0 12 0.0 0.0 0.0 0.02008435428800964 0.0 13 0.0 0.0 0.0 0.0223159492088996 0.0 14 0.0 0.0 0.0 0.0223159492088996 0.0 15 0.0 0.0 0.0 0.02677913905067952 0.0 16 0.0 0.0 0.0 0.02901073397156948 0.0 17 0.0 0.0 0.0 0.03124232889245944 0.0 18 0.0 0.0 0.0 0.0334739238133494 0.0 19 0.0 0.0 0.0 0.04686349333868916 0.0 20 0.0 0.0 0.0 0.049095088259579124 0.0 21 0.0 0.0 0.0 0.05132668318046908 0.0 22 0.0 0.0 0.0 0.05355827810135904 0.0 23 0.0 0.0 0.0 0.05355827810135904 0.0 24 0.0 0.0 0.0 0.055789873022249 0.0 25 0.0 0.0 0.0 0.06471625270580884 0.0 26 0.0 0.0 0.0 0.0669478476266988 0.0 27 0.0 0.0 0.0 0.08033741715203856 0.0 28 0.0 0.0 0.0 0.09819017651915825 0.0 29 0.0 0.0 0.0 0.10934815112360805 0.0 30 0.0 0.0 0.0 0.12050612572805784 0.0 31 0.0 0.0 0.0 0.15174845462051728 0.0 32 0.0 0.0 0.0 0.18075918859208676 0.0 33 0.0 0.0 0.0 0.2186963022472161 0.0 34 0.0 0.0 0.0 0.3168864787663743 0.0 35 0.0 0.0 0.0 0.37937113655129323 0.0 36 0.0 0.0 0.0 0.5199616165673607 0.0 37 0.0 0.0 0.0 0.6984892102385575 0.0 38 0.0 0.0 0.0 0.9238802972484434 0.0 39 0.0 0.0 0.0 1.2697775099863873 0.0 40 0.0 0.0 0.0 1.5933587735154315 0.0 41 0.0 0.0 0.0 1.8544553792595568 0.0 42 0.0 0.0 0.0 2.2583740599406394 0.0 43 0.0 0.0 0.0 2.6332820066501528 0.0 44 0.0 0.0 0.0 2.976947624467207 0.0 45 0.0 0.0 0.0 3.3764031153065095 0.0 46 0.0 0.0 0.0 3.791479770592042 0.0 47 0.0 0.0 0.0 4.202093236035795 0.0 48 0.0 0.0 0.0 4.626096271004887 0.0 49 0.0 0.0 0.0 5.003235812635291 0.0 50 0.0 0.0 0.0 5.512039454598201 0.0 51 0.0 0.0 0.0 5.944968869250854 0.0 52 0.0 0.0 0.0 6.478320055343554 0.0 53 0.0 0.0 0.0 6.830912052844168 0.0 54 0.0 0.0 0.0 7.212514784316351 0.0 55 0.0 0.0 0.0 7.582959541184084 0.0 56 0.0 0.0 0.0 7.944477918368258 0.0 57 0.0 0.0 0.0 8.31715427015688 0.0 58 0.0 0.0 0.0 8.663051482894826 0.0 59 0.0 0.0 0.0 9.042422619446118 0.0 60 0.0 0.0 0.0 9.381625047421393 0.0 61 0.0 0.0 0.0 9.743143424605565 0.0 62 0.0 0.0 0.0 10.133672535761308 0.0 63 0.0 0.0 0.0 10.508580482470823 0.0 64 0.0 0.0 0.0 10.841088125683426 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACAGG 20 0.0069109034 52.495815 18 TGAACTA 20 0.0069109034 52.495815 68 CACAGGT 20 0.0069109034 52.495815 19 GTATCAT 20 0.0069109034 52.495815 54 TCATCGA 20 0.0069109034 52.495815 45 CATCGAA 20 0.0069109034 52.495815 46 GACGAAT 30 5.821065E-4 46.662945 23 TTATCCA 40 4.52627E-5 43.746513 2 GAACATA 90 0.0052020643 19.442896 69 CTTATCC 95 0.0068824356 18.52295 1 CCCAACA 130 0.0021634912 16.2432 1 AACATAA 135 0.0028894425 15.554315 70 CCGACGA 265 9.13511E-6 13.206494 21 GCCGACG 275 1.3380792E-5 12.726258 20 CCCAACC 285 2.518742E-4 11.11377 1 CCCCACC 260 0.0014745959 10.8288 1 CGCTGCC 330 0.0010247632 9.544693 16 TGCCGAC 295 0.004407947 9.490768 19 GCTGCCG 305 0.005801782 9.179596 17 CTGCCGA 305 0.005801782 9.179596 18 >>END_MODULE