FastQCFastQC Report
Wed 25 May 2016
SRR1780410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44811
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT37828.439891990805828No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT36108.056057664412755No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC12632.8185043850840197No Hit
CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC5691.2697775099863873No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG3250.725268349289237No Hit
TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT3230.7208051594474572No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT1870.4173082502064225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1660.37044475686773337No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1460.3258128584499342No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT1450.3235812635290442No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1410.31465488384548435No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT1270.2834125549530249No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1220.27225458034857514No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT1200.26779139050679523No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT1110.24770703621878556No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT1000.22315949208899602No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC980.2186963022472161No Hit
CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC820.1829907835129767No Hit
TTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC750.167369619066747No Hit
CTTATTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG680.15174845462051728No Hit
CTTATCACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC580.1294325054116177No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTGGGATCTCGTATGCCGT540.12050612572805784No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG520.11604293588627793No Hit
ACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCT510.11381134096538796Illumina PCR Primer Index 3 (95% over 22bp)
TCTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT490.10934815112360805No Hit
CTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT470.10488496128182813No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTC470.10488496128182813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAC150.00221093670.02
CTTGACA150.00221093670.054
AATGAGG150.00221093670.032
CTATACA950.066.315791
TCTTTAT501.2732926E-1163.0000041
CTTTACA1800.062.2222251
TTTACAC1800.062.2222252
CTTTATA553.45608E-1157.272732
GAGGCGG200.00690872452.50000441
GCGGGCA200.00690872452.50000444
CAAGACG200.00690872452.50000459
CATGACA200.00690872452.50000449
GCCTCCG200.00690872452.50000420
ATCTCCC200.00690872452.50000470
GCAAGAC200.00690872452.50000458
AGAAGAC200.00690872452.50000466
CCTCGTG200.00690872452.5000047
AGGCGGG200.00690872452.50000442
TCGTGGA200.00690872452.5000049
GAAGACC200.00690872452.50000467
Produced by FastQC (version 0.11.5)