##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780410_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44811 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89857401084555 34.0 34.0 34.0 31.0 34.0 2 33.284572984311886 34.0 34.0 34.0 32.0 34.0 3 33.48200218696302 34.0 34.0 34.0 34.0 34.0 4 33.51741759835755 34.0 34.0 34.0 34.0 34.0 5 33.53681015822008 34.0 34.0 34.0 34.0 34.0 6 37.3086742094575 38.0 38.0 38.0 36.0 38.0 7 36.94414317913012 38.0 37.0 38.0 35.0 38.0 8 36.96389279418 38.0 37.0 38.0 35.0 38.0 9 36.68775523866908 38.0 37.0 38.0 34.0 38.0 10-11 36.42743969114726 38.0 37.0 38.0 34.0 38.0 12-13 36.31362835018187 38.0 37.0 38.0 34.0 38.0 14-15 36.39151101292093 38.0 37.0 38.0 34.0 38.0 16-17 36.476568253330655 38.0 37.0 38.0 34.0 38.0 18-19 36.54804623864676 38.0 37.0 38.0 34.0 38.0 20-21 36.34310771908683 38.0 37.0 38.0 34.0 38.0 22-23 36.356742764053465 38.0 37.0 38.0 33.5 38.0 24-25 35.508692062216866 38.0 36.0 38.0 31.0 38.0 26-27 35.72295864854611 38.0 36.0 38.0 31.0 38.0 28-29 35.476891834594184 38.0 36.0 38.0 30.5 38.0 30-31 35.23200776595033 37.5 36.0 38.0 29.0 38.0 32-33 35.59498783780768 38.0 36.0 38.0 31.0 38.0 34-35 35.099116288411324 38.0 36.0 38.0 28.5 38.0 36-37 35.07015018633818 38.0 35.5 38.0 27.0 38.0 38-39 34.31559215371226 37.0 34.0 38.0 25.0 38.0 40-41 34.79303072906206 37.0 34.5 38.0 28.5 38.0 42-43 35.67473388230569 37.5 36.5 38.0 32.5 38.0 44-45 35.86148490326036 38.0 36.5 38.0 32.5 38.0 46-47 36.06986007899846 38.0 37.0 38.0 33.0 38.0 48-49 35.74813103925376 38.0 36.5 38.0 31.5 38.0 50-51 35.83394702193658 38.0 36.5 38.0 31.5 38.0 52-53 35.699158688714824 38.0 36.0 38.0 31.0 38.0 54-55 35.9327955189574 38.0 37.0 38.0 33.0 38.0 56-57 35.89072995469863 38.0 37.0 38.0 32.5 38.0 58-59 35.828858985516945 38.0 37.0 38.0 32.0 38.0 60-61 35.667157617549265 38.0 36.5 38.0 31.5 38.0 62-63 36.08457744750173 38.0 37.0 38.0 33.5 38.0 64-65 36.02616545044744 38.0 37.0 38.0 33.0 38.0 66-67 34.86175269465087 38.0 35.0 38.0 26.0 38.0 68-69 32.72249001361273 37.0 32.0 38.0 16.0 38.0 70-71 31.229653433308783 37.0 26.5 38.0 11.0 38.0 72-73 29.79298609716364 36.0 22.5 38.0 11.0 38.0 74-75 29.917966570708085 37.0 23.0 38.0 11.0 38.0 76 29.737252014014416 36.0 23.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01066521025335021 1101 2 0.37941056285094277 1101 3 0.024976532856591405 1101 4 0.3805966370922249 1101 5 0.358454967232106 1101 6 -1.096482726026494 1101 7 0.18997969097085132 1101 8 0.18141372129746713 1101 9 0.34368799724015275 1101 10-11 0.11208150635369662 1101 12-13 0.64930348113608 1101 14-15 0.4981536759742653 1101 16-17 0.1352984479060737 1101 18-19 -0.4322848483453825 1101 20-21 0.0170160293665802 1101 22-23 0.5142704503413285 1101 24-25 -1.5750947154016544 1101 26-27 -0.18278913583900902 1101 28-29 0.2701929931644571 1101 30-31 0.26487308411838484 1101 32-33 0.2997789195066005 1101 34-35 -0.07497350554347548 1101 36-37 0.00978012190402211 1101 38-39 -0.008236193419598692 1101 40-41 1.139452261896956 1101 42-43 0.2936488197739706 1101 44-45 0.643623896241742 1101 46-47 0.7484072935645614 1101 48-49 -1.9265251915343384 1101 50-51 -1.900449020630525 1101 52-53 -1.3097529199109559 1101 54-55 -1.4285128475692872 1101 56-57 -1.3758035900334562 1101 58-59 -1.2342292134822301 1101 60-61 -1.844349350935154 1101 62-63 -1.2257214971475818 1101 64-65 -1.625287217676643 1101 66-67 -1.9798839431909272 1101 68-69 -2.5946392768809474 1101 70-71 -2.0786343132351917 1101 72-73 -2.9669028917020093 1101 74-75 -2.646126082432122 1101 76 -3.691884293898635 1102 1 -1.0437518057379584 1102 2 -0.14628420504224238 1102 3 0.1890814855036922 1102 4 0.13187749410200666 1102 5 -0.1294351119481476 1102 6 0.17086706437872579 1102 7 -0.06557068940487198 1102 8 0.3955109111748172 1102 9 0.9100596786560828 1102 10-11 0.6304016429784411 1102 12-13 0.6091699614962707 1102 14-15 -0.03390656311903939 1102 16-17 -0.11182932728411288 1102 18-19 -0.25956322126884146 1102 20-21 0.5636702756016376 1102 22-23 0.4596593651061127 1102 24-25 -5.407923468700837 1102 26-27 -1.2529257605867201 1102 28-29 0.36513166728338575 1102 30-31 1.4549056877513635 1102 32-33 0.1872187580409843 1102 34-35 0.5936338535622525 1102 36-37 -1.4905303888862278 1102 38-39 0.5954355966751095 1102 40-41 1.301577708256211 1102 42-43 0.6151439292290206 1102 44-45 1.2755521682491846 1102 46-47 0.949074891763594 1102 48-49 -3.194185492729808 1102 50-51 -0.5699879121070026 1102 52-53 -0.7221500632116928 1102 54-55 0.13289066120264437 1102 56-57 0.665300275825885 1102 58-59 -0.35773488030415734 1102 60-61 -6.469756896347359 1102 62-63 -3.094724757510882 1102 64-65 -0.5154052117769368 1102 66-67 0.8465017112105571 1102 68-69 -1.6905482972194008 1102 70-71 -1.6925529594081432 1102 72-73 -4.133103792183302 1102 74-75 -2.0686924541135348 1102 76 -0.6310241790096427 1103 1 0.577110851916558 1103 2 0.2317382324185573 1103 3 -0.09368626587251327 1103 4 0.17089916506570546 1103 5 0.049089032710760705 1103 6 0.14180985582182615 1103 7 0.5317396025608474 1103 8 0.4687462257870365 1103 9 0.3830580759803084 1103 10-11 0.057654099253262814 1103 12-13 -0.4967111617703992 1103 14-15 -0.3987243458519458 1103 16-17 0.1591622412476923 1103 18-19 0.8257618431865907 1103 20-21 -0.3572759999192314 1103 22-23 0.7798112708966514 1103 24-25 -3.256784175271811 1103 26-27 -0.32821668108006463 1103 28-29 -0.06811340274658306 1103 30-31 1.1888266688676623 1103 32-33 1.3143527556718198 1103 34-35 0.9708201123595472 1103 36-37 -0.7819038123410422 1103 38-39 0.186300344776285 1103 40-41 -0.5232428617389075 1103 42-43 -0.8541961864423584 1103 44-45 1.302658293257899 1103 46-47 0.8249372010103357 1103 48-49 -2.1218560383363823 1103 50-51 -0.5614519215836893 1103 52-53 0.3186833281896 1103 54-55 0.12107963758059981 1103 56-57 0.4322154733730912 1103 58-59 -0.6836006736286606 1103 60-61 -4.578808530379831 1103 62-63 -1.0165420524729072 1103 64-65 0.35174690305826317 1103 66-67 -0.32996682756722606 1103 68-69 0.8375682103410469 1103 70-71 0.22724357045040122 1103 72-73 -2.257067279048325 1103 74-75 -0.4040000981801519 1103 76 -1.1360064102130423 1104 1 -0.17098121349833661 1104 2 0.059842411703840526 1104 3 0.3041938068107086 1104 4 -0.14490943578226734 1104 5 -0.2407353161687098 1104 6 0.0699140351071108 1104 7 -0.002058095803782578 1104 8 0.21920049592635138 1104 9 0.9436879972401542 1104 10-11 -0.38886316301202584 1104 12-13 -0.10791649187336105 1104 14-15 0.5709606935181313 1104 16-17 0.38766012132037986 1104 18-19 0.7001712920054999 1104 20-21 -0.7895291798102093 1104 22-23 -0.1569846103874184 1104 24-25 0.169169792020746 1104 26-27 0.9157263837291367 1104 28-29 1.3959689715720174 1104 30-31 -0.024195741792546244 1104 32-33 0.922923318966788 1104 34-35 0.44162568473992536 1104 36-37 1.291021687356114 1104 38-39 1.2960822951094215 1104 40-41 1.4308152848389213 1104 42-43 0.3647014513529214 1104 44-45 0.8818830055534832 1104 46-47 1.1229686970733255 1104 48-49 -0.06168038721585134 1104 50-51 0.8305226392885103 1104 52-53 0.02958581153303186 1104 54-55 0.052121430433409444 1104 56-57 0.4563826447843624 1104 58-59 -0.5050119395281101 1104 60-61 0.22285712679763492 1104 62-63 0.10241615467428034 1104 64-65 0.6272768848874648 1104 66-67 -0.4422321213285798 1104 68-69 1.758464636749288 1104 70-71 1.7364803966163613 1104 72-73 0.3945680934531879 1104 74-75 0.5769508406448018 1104 76 1.0770765697990718 1105 1 0.85746243587554 1105 2 0.5348573192768882 1105 3 0.318326186059366 1105 4 -0.0257681119897768 1105 5 0.11018895336021473 1105 6 0.07283591942434242 1105 7 0.7260874021091581 1105 8 -0.027072610811870845 1105 9 0.9793469119688325 1105 10-11 0.4333789359742539 1105 12-13 -0.710957636774971 1105 14-15 0.5163095307274261 1105 16-17 0.31267502889342325 1105 18-19 0.7517216796023902 1105 20-21 0.4931482203090525 1105 22-23 0.034160291222598005 1105 24-25 0.8485078957774519 1105 26-27 0.7232696156909739 1105 28-29 1.2087894843925326 1105 30-31 1.256305152661838 1105 32-33 0.9415577852756769 1105 34-35 -0.918302758909455 1105 36-37 -0.2845895231388198 1105 38-39 -0.4518306446639926 1105 40-41 0.47431558299037846 1105 42-43 0.46082548236066856 1105 44-45 1.0806903997097095 1105 46-47 1.2202555187787496 1105 48-49 0.36172450246811394 1105 50-51 0.9977557937309669 1105 52-53 0.24461264516451564 1105 54-55 0.865448800737532 1105 56-57 0.15775414150469658 1105 58-59 0.46222121491434365 1105 60-61 0.4003466914965017 1105 62-63 -0.5477329210561948 1105 64-65 0.7332399141063064 1105 66-67 0.6191572644794121 1105 68-69 -0.6522688158446357 1105 70-71 0.11975409965750217 1105 72-73 -0.8296119900292211 1105 74-75 -1.7837229816569256 1105 76 -1.1349687492229918 1106 1 -1.701081547946501 1106 2 0.12204976956337532 1106 3 0.1767690576468297 1106 4 0.1815119689000113 1106 5 0.17699043633965061 1106 6 0.31038226253185996 1106 7 -0.39369689179040535 1106 8 0.24930083037450856 1106 9 -1.012833741890283 1106 10-11 0.2636451981919876 1106 12-13 1.0539564178256313 1106 14-15 -0.5094740890905669 1106 16-17 0.03465008787556201 1106 18-19 0.5762582485272318 1106 20-21 0.45378185697909146 1106 22-23 0.6461926471376671 1106 24-25 -0.06109776650768595 1106 26-27 0.6774320693478728 1106 28-29 0.36820977424759604 1106 30-31 0.6955366996790247 1106 32-33 0.47994673033802826 1106 34-35 0.9723949155091489 1106 36-37 1.3448678412022659 1106 38-39 -0.6057571697735256 1106 40-41 0.32797247547437536 1106 42-43 0.7212231904833502 1106 44-45 0.966163942008329 1106 46-47 0.929490436203757 1106 48-49 0.9930018870316388 1106 50-51 -0.042554283788412306 1106 52-53 0.7085155774193268 1106 54-55 0.4502819655504666 1106 56-57 0.5446769591656277 1106 58-59 0.9740850504384397 1106 60-61 1.2049641502090083 1106 62-63 0.8716469239050468 1106 64-65 -1.0593451886911325 1106 66-67 0.4298414572667326 1106 68-69 1.7039495865820626 1106 70-71 0.5985205303956249 1106 72-73 0.08771190616221602 1106 74-75 -0.07438695199734013 1106 76 0.1961401149495714 1107 1 0.8358443552990593 1107 2 -0.1325736229657153 1107 3 0.10083519359293547 1107 4 0.32388796400105235 1107 5 0.23651278783996332 1107 6 0.2704557469164257 1107 7 0.6587219692016362 1107 8 -0.011568734842882122 1107 9 -0.3746218619147754 1107 10-11 0.538384940996643 1107 12-13 0.09284190465967868 1107 14-15 0.3815240737998238 1107 16-17 0.6628497204536359 1107 18-19 0.7543966441181666 1107 20-21 0.9422693033967278 1107 22-23 -0.12453607300930258 1107 24-25 0.24582201303916662 1107 26-27 -0.8031251872351106 1107 28-29 0.8378974656131888 1107 30-31 0.4637240848596704 1107 32-33 0.9768236439938747 1107 34-35 -0.12408395773028502 1107 36-37 1.1293748834557888 1107 38-39 -0.01188086848754466 1107 40-41 0.45660076696243124 1107 42-43 0.7780817330430594 1107 44-45 0.6406587368644168 1107 46-47 1.0835320773550237 1107 48-49 0.5444409562294368 1107 50-51 1.250403462690464 1107 52-53 0.7876031463109356 1107 54-55 1.0835948865547564 1107 56-57 1.056924356593676 1107 58-59 0.4366998697893294 1107 60-61 1.3055765200804146 1107 62-63 1.010379318271248 1107 64-65 1.1833982283327487 1107 66-67 -0.019913594784704003 1107 68-69 0.8170236833364939 1107 70-71 -1.2621653238603479 1107 72-73 -1.0031025457667475 1107 74-75 -2.3573936880659225 1107 76 -0.9141476988899875 1108 1 -0.7142860568031821 1108 2 0.21995637181780126 1108 3 -0.4670312644143664 1108 4 0.3154894246165938 1108 5 -0.01366978910318295 1108 6 -0.040342375149300835 1108 7 5.060067603182006E-4 1108 8 -0.4930502163243631 1108 9 -0.37483052127836913 1108 10-11 0.12338754923868578 1108 12-13 1.0362430522861743 1108 14-15 0.4061458787033132 1108 16-17 -0.3837074000471432 1108 18-19 -0.1535324116982153 1108 20-21 0.6450862048051746 1108 22-23 -0.3146769180797264 1108 24-25 0.6419618148127668 1108 26-27 -1.2129060949033388 1108 28-29 -1.1763957007926606 1108 30-31 -0.06807038566719115 1108 32-33 1.0857438317872976 1108 34-35 0.44276528587952413 1108 36-37 1.2930159893504154 1108 38-39 0.8953700443971613 1108 40-41 0.2896471936708309 1108 42-43 -0.09455780790634805 1108 44-45 1.039432863103336 1108 46-47 0.576672400777035 1108 48-49 0.5079777324422636 1108 50-51 0.6722605310264029 1108 52-53 0.09482797677519983 1108 54-55 -0.5374797091677266 1108 56-57 0.16464475304646697 1108 58-59 0.28487410035792493 1108 60-61 0.8229995769400844 1108 62-63 0.6086839609420807 1108 64-65 0.6210090786196574 1108 66-67 0.3348334057369442 1108 68-69 0.772709650994301 1108 70-71 -1.9058272956913314 1108 72-73 -1.8639789150938206 1108 74-75 -2.2850149713210115 1108 76 -1.1468550541325548 1109 1 -0.8026179977667027 1109 2 0.5423178310213643 1109 3 0.25555297395934673 1109 4 0.19262671228019457 1109 5 0.3396579747508994 1109 6 -0.21678347790619057 1109 7 -0.32380477018203635 1109 8 0.34181472380373634 1109 9 0.546695516037147 1109 10-11 0.39528952640382187 1109 12-13 -0.022375716858910266 1109 14-15 0.6472764829918134 1109 16-17 0.6960753902619672 1109 18-19 0.8321261792235433 1109 20-21 1.1398230469104362 1109 22-23 0.474796766130801 1109 24-25 -0.7098064948502767 1109 26-27 -0.2686287348365042 1109 28-29 -1.533065152198951 1109 30-31 0.3212640615619975 1109 32-33 0.695768894443944 1109 34-35 -0.8456501972727963 1109 36-37 1.157649796089487 1109 38-39 1.2994079168560901 1109 40-41 0.6576226879621174 1109 42-43 1.3030473160145704 1109 44-45 0.8823457007530138 1109 46-47 0.6744724564718254 1109 48-49 0.5446527533278172 1109 50-51 0.5168442124521917 1109 52-53 -0.09609377204128577 1109 54-55 -0.33703415082742794 1109 56-57 -0.03181862762743748 1109 58-59 -1.014930967868196 1109 60-61 0.5635453859069557 1109 62-63 0.4791657208975266 1109 64-65 0.683610025431129 1109 66-67 0.6761310944030541 1109 68-69 -0.6366192267556343 1109 70-71 0.29793210280491067 1109 72-73 0.1483853288493684 1109 74-75 0.23169346644005984 1109 76 0.48355430259259435 1110 1 -0.15472811176136503 1110 2 -0.16869356844504324 1110 3 -0.03501214852428092 1110 4 -0.0544627856581954 1110 5 0.25616294685858776 1110 6 -0.2586219450417744 1110 7 0.25265654439473195 1110 8 0.7061732006409898 1110 9 0.7469138036917684 1110 10-11 -0.8976100612750528 1110 12-13 0.7126170068859423 1110 14-15 0.7338639193245768 1110 16-17 0.4397693024618121 1110 18-19 0.009848711360326945 1110 20-21 0.7692797531922793 1110 22-23 -0.2945156277571499 1110 24-25 -0.5380262377559362 1110 26-27 1.1378107509747935 1110 28-29 0.5582518499839253 1110 30-31 -0.152359513504706 1110 32-33 0.5634320038303144 1110 34-35 0.8522336251865781 1110 36-37 1.6577710670087171 1110 38-39 0.8695934613625198 1110 40-41 -0.3500780154092169 1110 42-43 0.09776596748194777 1110 44-45 0.9494388368189917 1110 46-47 0.976194503524944 1110 48-49 1.4844114241985409 1110 50-51 -0.8998123483045433 1110 52-53 0.7669307246843928 1110 54-55 -0.5404964355715478 1110 56-57 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46-47 -1.0431027314981023 2116 48-49 0.06908884355338074 2116 50-51 -1.6800542837884151 2116 52-53 -0.19101858407136518 2116 54-55 -0.8626450530830638 2116 56-57 -0.27500471785300107 2116 58-59 -2.8405888626050384 2116 60-61 -2.423296719356209 2116 62-63 -0.11763879038066705 2116 64-65 -1.4303467414861686 2116 66-67 -0.3525343191307826 2116 68-69 -1.1899556929210462 2116 70-71 1.419172704308668 2116 72-73 1.4343345769696718 2116 74-75 0.9386953461393084 2116 76 0.029137009359509136 2117 1 0.6891997819767752 2117 2 0.4299781583101776 2117 3 -0.21496154763876518 2117 4 -0.2886500089346029 2117 5 0.23513195381620733 2117 6 0.4675007621056011 2117 7 0.7142314969564012 2117 8 0.4394116573139826 2117 9 -1.3621943557010212 2117 10-11 0.012494303051319378 2117 12-13 -0.8891382113303834 2117 14-15 -0.3202157770701035 2117 16-17 -0.7824002105046617 2117 18-19 -1.3910051785827378 2117 20-21 -0.13236477558697857 2117 22-23 0.30787210152811895 2117 24-25 -2.0781906906338534 2117 26-27 -2.6740172060144474 2117 28-29 -1.8892497312937522 2117 30-31 -0.8068503420942008 2117 32-33 -2.4417071486048556 2117 34-35 0.5047478566856256 2117 36-37 -1.4992498058565573 2117 38-39 -0.19373670941546806 2117 40-41 -0.6415074904250275 2117 42-43 0.05783870625487708 2117 44-45 -0.8328982915807615 2117 46-47 -0.9515411068482393 2117 48-49 -0.6416954701721096 2117 50-51 -0.6347111465335118 2117 52-53 0.0950458416989477 2117 54-55 -1.4259328682091237 2117 56-57 -0.09406984390342643 2117 58-59 -1.4153437645658258 2117 60-61 -1.9919241703366026 2117 62-63 -0.8469805270753454 2117 64-65 -1.551757805911933 2117 66-67 -1.7046001454613133 2117 68-69 -0.01182193941964016 2117 70-71 -0.2401410211815289 2117 72-73 2.039289058762389 2117 74-75 1.216619015607094 2117 76 1.3132756648216954 2118 1 -0.5161379086550326 2118 2 0.35699340885484077 2118 3 -0.4077720051551026 2118 4 -0.07895501982785191 2118 5 0.35114502571163797 2118 6 -0.03849052329744751 2118 7 0.7338393400936525 2118 8 0.13657941330526313 2118 9 -1.6007564472042901 2118 10-11 -1.2590198581687204 2118 12-13 -1.8248680588249329 2118 14-15 0.04225698981442605 2118 16-17 -3.6707444370841813 2118 18-19 -0.6766805598463606 2118 20-21 -1.2363952766763049 2118 22-23 0.5325453041425021 2118 24-25 0.9697395925905425 2118 26-27 -2.576794983792226 2118 28-29 -0.5458401452371007 2118 30-31 -0.38936668196348734 2118 32-33 -0.004070983027517627 2118 34-35 -0.22945693634269304 2118 36-37 -3.7977247513903265 2118 38-39 -0.22314847412135208 2118 40-41 -0.32887132484768955 2118 42-43 0.5804421920936562 2118 44-45 -3.588344914674444 2118 46-47 -0.45943871033407646 2118 48-49 0.45334810281264026 2118 50-51 -1.3953320615661937 2118 52-53 -2.1440609121136873 2118 54-55 -1.5847019313899509 2118 56-57 0.252607716009436 2118 58-59 -0.9632740477902217 2118 60-61 -1.0807041267636137 2118 62-63 -1.602427150169028 2118 64-65 -0.14843536582478833 2118 66-67 0.7774259983295337 2118 68-69 -2.2846977564131095 2118 70-71 -0.6132347030987404 2118 72-73 -0.13064558176048635 2118 74-75 0.580725769419729 2118 76 -0.9764846837621839 2119 1 0.7014546839375555 2119 2 -0.9983306652192354 2119 3 0.0019502170671188424 2119 4 -0.5870568716796996 2119 5 -0.468299418732812 2119 6 -0.47367570848263085 2119 7 -1.1724106599063475 2119 8 0.030097931823782176 2119 9 -1.020895336093183 2119 10-11 -0.484135598909468 2119 12-13 -0.09165046623234474 2119 14-15 -0.44674763981520016 2119 16-17 -0.7569712889360289 2119 18-19 0.1022546253388299 2119 20-21 -1.301788795194824 2119 22-23 -0.21571858474639782 2119 24-25 1.1647627407386878 2119 26-27 0.14889946065221693 2119 28-29 -0.6922521822741388 2119 30-31 0.4057722069254055 2119 32-33 0.21699383178729903 2119 34-35 0.018228248842483197 2119 36-37 -0.632215492131067 2119 38-39 -2.238773474121352 2119 40-41 -1.5661398433662086 2119 42-43 -0.12904854864708426 2119 44-45 0.16802082606630364 2119 46-47 0.06255203040665691 2119 48-49 -0.49861948977994786 2119 50-51 0.46057071621158485 2119 52-53 0.4265409397381603 2119 54-55 0.13809899453597296 2119 56-57 -2.2549154321387164 2119 58-59 -0.752047195938367 2119 60-61 -0.06913005268953754 2119 62-63 -0.28876974276162315 2119 64-65 0.4424211156566926 2119 66-67 0.3364537761073194 2119 68-69 0.2534966880313405 2119 70-71 -0.37770229569133207 2119 72-73 1.6158821960172887 2119 74-75 1.504915584234542 2119 76 1.7225893903118923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 6.0 23 8.0 24 44.0 25 75.0 26 152.0 27 315.0 28 554.0 29 922.0 30 1322.0 31 1925.0 32 2927.0 33 4238.0 34 5815.0 35 6421.0 36 7182.0 37 12902.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.007007208051597 25.884269487402644 10.943741492044364 37.164981812501395 2 13.166410033250763 15.380152194773606 52.59199750061369 18.861440271361943 3 12.113097230590702 29.892213965321012 39.68891566802794 18.305773136060342 4 9.187476289303966 26.431010243020687 33.741715203856195 30.639798263819152 5 8.013657360915847 43.3643525027337 37.252014014416105 11.369976121934346 6 16.66331927428533 40.90513489991297 15.422552498270514 27.00899332753119 7 16.66331927428533 40.51237419383634 17.415366762625247 25.408939769253085 8 15.10343442458325 36.65617817053848 26.022628372497824 22.217759032380442 9 14.730758072794625 20.117828211822992 16.473633706009686 48.677780009372704 10-11 15.713775635446654 24.864430608555935 33.14253196759724 26.27926178840017 12-13 21.278257570685767 16.363169757425634 28.65033139184575 33.70824128004284 14-15 23.330209100444087 22.60047756131307 18.207582959541185 35.86173037870166 16-17 25.232643770502776 27.345964160585574 16.709066970163576 30.712325098748078 18-19 22.33157037334583 26.16545044743478 17.38635602865368 34.11662315056571 20-21 20.723706232844613 21.947736046952755 17.42764053469014 39.90091718551248 22-23 23.83008636272344 31.58041552297427 17.630715672491128 26.958782441811163 24-25 25.000557898730225 34.3844145410725 17.52806230613019 23.08696525406708 26-27 27.7956305371449 35.47454865992725 17.849411974738345 18.880408828189505 28-29 25.169043315257415 28.58226774675861 24.16817299323827 22.08051594474571 30-31 36.520050880364195 19.567740063823617 27.938452612081853 15.973756443730332 32-33 32.71406574278637 18.429626654169734 33.99388542991676 14.862422173127134 34-35 34.38329874361206 24.175983575461384 26.405346901430455 15.035370779496107 36-37 24.884514962843944 27.122804668496574 26.619580013835886 21.373100354823592 38-39 20.82524380174511 21.25371002655598 28.093548459083706 29.82749771261521 40-41 17.53698868581375 23.50538930173395 32.88031956439267 26.07730244805963 42-43 26.765749481154185 21.078529825266116 34.66001651380242 17.495704179777285 44-45 18.132824529691373 24.535270357724663 29.661243891008905 27.67066122157506 46-47 27.436343754881616 18.024592176028207 26.94762446720671 27.591439601883465 48-49 23.011091026756823 17.977728682689516 28.045569168284572 30.965611122269088 50-51 15.634554015755059 17.51244114168396 35.583896811050856 31.269108031510118 52-53 18.624891209747606 17.393050813416348 42.431545825801706 21.550512151034347 54-55 16.334159023454063 17.525830711209302 36.84474794135368 29.29526232398295 56-57 25.638794046104753 17.410903572783468 31.00020084354288 25.950101537568898 58-59 17.177701903550467 29.836424092298763 34.82961772779005 18.156256276360715 60-61 20.047532971814956 42.31550288991542 21.28718395036933 16.349780187900294 62-63 13.330432259936176 51.853339581799105 18.07257146682734 16.74365669143737 64-65 12.610742897949164 53.30499207783803 17.158733346722904 16.925531677489904 66-67 12.839481377340386 51.84999218941778 17.470040838187053 17.840485595054787 68-69 12.965566490370668 48.309566847425856 18.030171163330433 20.694695498873045 70-71 14.036732052397848 44.28042221775903 18.956283055499764 22.726562674343352 72-73 14.287786480997969 39.49141951752918 20.005132668318044 26.215661333154806 74-75 15.182656044274841 38.705898105375915 19.885742340050435 26.22570351029881 76 14.355850126085112 39.405503113074914 20.07765950324697 26.160987257593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 1.0 17 0.0 18 0.5 19 2.0 20 6.5 21 10.0 22 9.5 23 10.0 24 17.5 25 24.0 26 54.5 27 102.5 28 149.0 29 178.0 30 189.5 31 230.5 32 335.0 33 410.0 34 474.0 35 615.5 36 771.0 37 849.0 38 1026.5 39 1383.0 40 1933.0 41 3393.5 42 4483.0 43 5240.0 44 6011.5 45 5461.5 46 4897.0 47 4319.5 48 3270.0 49 2401.5 50 2005.0 51 1811.0 52 1404.5 53 1055.5 54 919.0 55 920.0 56 742.0 57 488.0 58 413.0 59 344.0 60 232.0 61 154.5 62 120.0 63 91.5 64 55.0 65 39.0 66 30.5 67 30.0 68 21.5 69 7.5 70 2.5 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 44811.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.59121644239138 #Duplication Level Percentage of deduplicated Percentage of total 1 61.54348471356486 23.134944544866215 2 17.90442267735233 13.460980562808238 3 8.679133273968537 9.787775323023364 4 4.31582071831404 6.489478029948003 5 2.831700801424755 5.322353886322555 6 1.5553576728999703 3.5080672156390174 7 0.9201543484713566 2.4212804891656066 8 0.5342831700801425 1.6067483430407714 9 0.3918076580587711 1.3255673830086363 >10 1.1813594538438705 6.665774028698311 >50 0.053428317008014245 1.4237575595277945 >100 0.06530127634312852 4.2690410836624935 >500 0.005936479667557139 1.2697775099863873 >1k 0.017809439002671415 19.314454040302603 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT 3782 8.439891990805828 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT 3610 8.056057664412755 No Hit CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 1263 2.8185043850840197 No Hit CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 569 1.2697775099863873 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 325 0.725268349289237 No Hit TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT 323 0.7208051594474572 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT 187 0.4173082502064225 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 166 0.37044475686773337 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 146 0.3258128584499342 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT 145 0.3235812635290442 No Hit CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 141 0.31465488384548435 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT 127 0.2834125549530249 No Hit CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 122 0.27225458034857514 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT 120 0.26779139050679523 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT 111 0.24770703621878556 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT 100 0.22315949208899602 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 98 0.2186963022472161 No Hit CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 82 0.1829907835129767 No Hit TTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 75 0.167369619066747 No Hit CTTATTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 68 0.15174845462051728 No Hit CTTATCACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 58 0.1294325054116177 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTGGGATCTCGTATGCCGT 54 0.12050612572805784 No Hit CTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 52 0.11604293588627793 No Hit ACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCT 51 0.11381134096538796 Illumina PCR Primer Index 3 (95% over 22bp) TCTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT 49 0.10934815112360805 No Hit CTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT 47 0.10488496128182813 No Hit CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTC 47 0.10488496128182813 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00223159492088996 0.0 2 0.0 0.0 0.0 0.00446318984177992 0.0 3 0.0 0.0 0.0 0.00892637968355984 0.0 4 0.0 0.0 0.0 0.0111579746044498 0.0 5 0.0 0.0 0.0 0.01562116444622972 0.0 6 0.0 0.0 0.0 0.01562116444622972 0.0 7 0.0 0.0 0.0 0.01562116444622972 0.0 8 0.0 0.0 0.0 0.01562116444622972 0.0 9 0.0 0.0 0.0 0.02008435428800964 0.0 10 0.0 0.0 0.0 0.02008435428800964 0.0 11 0.0 0.0 0.0 0.02008435428800964 0.0 12 0.0 0.0 0.0 0.0223159492088996 0.0 13 0.0 0.0 0.0 0.024547544129789562 0.0 14 0.0 0.0 0.0 0.02677913905067952 0.0 15 0.0 0.0 0.0 0.03124232889245944 0.0 16 0.0 0.0 0.0 0.0334739238133494 0.0 17 0.0 0.0 0.0 0.03793711365512932 0.0 18 0.0 0.0 0.0 0.04016870857601928 0.0 19 0.0 0.0 0.0 0.055789873022249 0.0 20 0.0 0.0 0.0 0.05802146794313896 0.0 21 0.0 0.0 0.0 0.06248465778491888 0.0 22 0.0 0.0 0.0 0.06248465778491888 0.0 23 0.0 0.0 0.0 0.06248465778491888 0.0 24 0.0 0.0 0.0 0.06471625270580884 0.0 25 0.0 0.0 0.0 0.08033741715203856 0.0 26 0.0 0.0 0.0 0.08480060699381849 0.0 27 0.0 0.0 0.0 0.09372698667737832 0.0 28 0.0 0.0 0.0 0.10488496128182813 0.0 29 0.0 0.0 0.0 0.11604293588627793 0.0 30 0.0 0.0 0.0 0.12720091049072774 0.0 31 0.0 0.0 0.0 0.1606748343040771 0.0 32 0.0 0.0 0.0 0.18745397335475664 0.0 33 0.0 0.0 0.0 0.22762268193077592 0.0 34 0.0 0.0 0.0 0.30572850416192454 0.0 35 0.0 0.0 0.0 0.37267635178862335 0.0 36 0.0 0.0 0.0 0.508803641962911 0.0 37 0.0 0.0 0.0 0.6984892102385575 0.0 38 0.0 0.0 0.0 0.9127223226439937 0.0 39 0.0 0.0 0.0 1.2764722947490572 0.0 40 0.0 0.0 0.0 1.6290642922496708 0.0 41 0.0 0.0 0.0 1.9191716319653656 0.0 42 0.0 0.0 0.0 2.3364798821717883 0.0 43 0.0 0.0 0.0 2.735935373011091 0.0 44 0.0 0.0 0.0 3.1041485349579343 0.0 45 0.0 0.0 0.0 3.5147620004016873 0.0 46 0.0 0.0 0.0 3.9365334404498897 0.0 47 0.0 0.0 0.0 4.3806208297069915 0.0 48 0.0 0.0 0.0 4.835866193568544 0.0 49 0.0 0.0 0.0 5.226395304724287 0.0 50 0.0 0.0 0.0 5.779830845104996 0.0 51 0.0 0.0 0.0 6.214991854678539 0.0 52 0.0 0.0 0.0 6.772890584901028 0.0 53 0.0 0.0 0.0 7.145566936689652 0.0 54 0.0 0.0 0.0 7.542790832608065 0.0 55 0.0 0.0 0.0 7.926625159001138 0.0 56 0.0 0.0 0.0 8.32608064984044 0.0 57 0.0 0.0 0.0 8.723304545758854 0.0 58 0.0 0.0 0.0 9.093749302626588 0.0 59 0.0 0.0 0.0 9.51105755283301 0.0 60 0.0 0.0 0.0 9.848028385887394 0.0 61 0.0 0.0 0.0 10.225167927517797 0.0 62 0.0 0.0 0.0 10.638012987882439 0.0 63 0.0 0.0 0.0 11.046394858405302 0.0 64 0.0 0.0 0.0 11.412376425431257 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTAAC 15 0.002210936 70.0 2 CTTGACA 15 0.002210936 70.0 54 AATGAGG 15 0.002210936 70.0 32 CTATACA 95 0.0 66.31579 1 TCTTTAT 50 1.2732926E-11 63.000004 1 CTTTACA 180 0.0 62.222225 1 TTTACAC 180 0.0 62.222225 2 CTTTATA 55 3.45608E-11 57.27273 2 GAGGCGG 20 0.006908724 52.500004 41 GCGGGCA 20 0.006908724 52.500004 44 CAAGACG 20 0.006908724 52.500004 59 CATGACA 20 0.006908724 52.500004 49 GCCTCCG 20 0.006908724 52.500004 20 ATCTCCC 20 0.006908724 52.500004 70 GCAAGAC 20 0.006908724 52.500004 58 AGAAGAC 20 0.006908724 52.500004 66 CCTCGTG 20 0.006908724 52.500004 7 AGGCGGG 20 0.006908724 52.500004 42 TCGTGGA 20 0.006908724 52.500004 9 GAAGACC 20 0.006908724 52.500004 67 >>END_MODULE