FastQCFastQC Report
Wed 25 May 2016
SRR1780409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241209
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA207.8373373E-444.00768310
AGCGTCC250.002332742335.25005318
AAGCGTC250.002337505635.23540517
GATATCT250.0023398935.22808539
ATGCCAA250.0023398935.22808540
TCCAATC250.002344664735.21345522
CTTCCGA250.002347055135.20614611
TCCCATG250.002351841435.19153625
AGATTCA250.002359035235.1696431
CGCAACA250.002359035235.1696433
CTTAGAC353.1913986E-431.460181
CAGCGGG502.3327302E-630.830981
GATATCG502.366989E-630.77343441
CGTCCAA300.00569050929.3811520
GCGTCCA300.00569050929.3811519
GCAACAA300.00571368229.35673734
GACGAAT300.005725296729.34454523
CTATAGA300.00573111129.33845311
TTCCGAA300.00573111129.33845312
GCGCAAC300.00576025229.30803132
Produced by FastQC (version 0.11.5)