FastQCFastQC Report
Wed 25 May 2016
SRR1780408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77081
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATC150.002217910369.9928634
GCAAGAT150.002217910369.9928623
GTGTCAA150.002217910369.9928650
CATAAGT150.002217910369.9928614
TTTTAGA150.002217910369.9928628
AAAGAGG150.002217910369.9928630
TCAATAC252.3782131E-455.994293
GTCATAA200.00693036452.49464812
TGGAGTC200.00693036452.49464820
TACTTGG200.00693036452.4946487
GTTGTAA200.00693036452.49464870
GTCCGTA200.00693036452.49464824
GCTAGTC200.00693036452.49464865
TCCGTAA200.00693036452.49464825
AGCTAGT200.00693036452.49464864
GCATGGT200.00693036452.49464816
CCCATGA200.00693036452.49464834
GAAGGTC200.00693036452.49464836
ATTGAGG305.644912E-446.9973831
TACGGAT305.8480696E-446.6619077
Produced by FastQC (version 0.11.5)