##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780408_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 77081 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.186621865310517 34.0 23.0 34.0 12.0 34.0 2 28.94421452757489 34.0 23.0 34.0 12.0 34.0 3 29.22528249503769 34.0 27.0 34.0 11.0 34.0 4 29.54859174115541 34.0 31.0 34.0 11.0 34.0 5 29.52800300982084 34.0 31.0 34.0 11.0 34.0 6 31.612641247518845 37.0 27.0 38.0 11.0 38.0 7 31.091734668725106 36.0 27.0 38.0 11.0 38.0 8 30.72541871537733 36.0 26.0 38.0 11.0 38.0 9 31.11247908044784 37.0 27.0 38.0 12.0 38.0 10-11 31.29358726534425 37.0 27.0 38.0 11.0 38.0 12-13 31.582108431390353 37.0 27.5 38.0 11.0 38.0 14-15 31.21430054098935 37.0 27.0 38.0 11.0 38.0 16-17 31.00203033172896 37.0 26.0 38.0 11.0 38.0 18-19 31.07215137323076 37.0 26.5 38.0 11.0 38.0 20-21 31.19026089438383 37.0 26.5 38.0 11.0 38.0 22-23 31.005812067824756 37.0 26.0 38.0 11.0 38.0 24-25 31.406500953542377 37.0 27.0 38.0 11.0 38.0 26-27 31.352557699043864 37.0 27.0 38.0 11.0 38.0 28-29 31.379749873509684 37.0 27.0 38.0 11.0 38.0 30-31 31.25353848548929 37.0 27.0 38.0 11.0 38.0 32-33 31.13210129603923 37.0 26.5 38.0 11.0 38.0 34-35 31.123175620451214 37.0 26.0 38.0 11.0 38.0 36-37 31.0252656296623 37.0 26.0 38.0 11.0 38.0 38-39 31.344365018616777 37.0 27.0 38.0 11.0 38.0 40-41 30.995751222739713 37.0 26.0 38.0 11.0 38.0 42-43 31.32163568194497 37.0 27.0 38.0 11.0 38.0 44-45 30.511877116280278 36.0 25.5 38.0 11.0 38.0 46-47 31.12148259622994 37.0 26.5 38.0 11.0 38.0 48-49 30.997593440666314 37.0 26.0 38.0 11.0 38.0 50-51 30.96079448891426 37.0 26.5 38.0 11.0 38.0 52-53 30.93881112076906 37.0 26.0 38.0 11.0 38.0 54-55 30.748751313553274 36.5 26.0 38.0 11.0 38.0 56-57 30.702079630518547 36.5 25.5 38.0 11.0 38.0 58-59 30.585196092422258 36.5 25.5 38.0 11.0 38.0 60-61 30.642661615702963 36.5 25.0 38.0 11.0 38.0 62-63 30.641954567273388 36.5 25.0 38.0 11.0 38.0 64-65 30.7077295312723 37.0 25.0 38.0 11.0 38.0 66-67 30.784817270144394 36.5 25.0 38.0 11.0 38.0 68-69 30.455812716493043 36.0 24.5 38.0 11.0 38.0 70-71 30.392483231924857 36.0 25.0 38.0 11.0 38.0 72-73 30.45322453003983 36.0 24.5 38.0 11.0 38.0 74-75 29.965815181432518 36.0 24.0 38.0 11.0 38.0 76 29.091202760732216 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.935480914289844 1101 2 -1.3279413035723557 1101 3 -1.452699038365818 1101 4 -3.0444820486488915 1101 5 -2.0102830438282524 1101 6 -3.4109496686367606 1101 7 -1.7847113866839628 1101 8 -0.7826379882918388 1101 9 -1.450047797635225 1101 10-11 -1.341107657443942 1101 12-13 -1.9408325282566317 1101 14-15 -1.3806009949955858 1101 16-17 -2.436157815841103 1101 18-19 -0.9802512808832695 1101 20-21 -1.5521382659952145 1101 22-23 -0.5109694736624348 1101 24-25 -2.4897733650866662 1101 26-27 -1.2221056664933094 1101 28-29 -1.7189360478590814 1101 30-31 -1.4681470915407786 1101 32-33 -1.172133562146744 1101 34-35 -2.2565101879802647 1101 36-37 -1.3145447025544463 1101 38-39 -1.812766986233406 1101 40-41 -1.2927374510725258 1101 42-43 -1.5639115389257512 1101 44-45 -1.1246929773973449 1101 46-47 -0.8699152800093053 1101 48-49 -2.4862299048693117 1101 50-51 -2.1172407757252643 1101 52-53 -1.595354467331532 1101 54-55 -2.4155714874379655 1101 56-57 -1.623652094852904 1101 58-59 -1.2549205852079162 1101 60-61 -1.596041305354337 1101 62-63 -2.364566078079072 1101 64-65 -1.5428558777266943 1101 66-67 -1.9780370002008176 1101 68-69 -1.1533709044833103 1101 70-71 -0.6409975965055175 1101 72-73 -1.545757279577174 1101 74-75 -1.942103316073915 1101 76 -2.892996788651921 1102 1 0.33478935598042625 1102 2 -0.46050887113992545 1102 3 -0.030942281609060984 1102 4 -0.08731988648672839 1102 5 0.39079803725283213 1102 6 -0.22878750647459611 1102 7 1.2613696943971213 1102 8 1.2099295792757268 1102 9 0.5980603104728814 1102 10-11 -0.49914819798448207 1102 12-13 -3.9234000958241957 1102 14-15 -1.3170874814820728 1102 16-17 -2.254671329354615 1102 18-19 -1.458832361964351 1102 20-21 0.14772659886964945 1102 22-23 -0.4197532574462173 1102 24-25 0.2271860943727937 1102 26-27 -5.38440296379061 1102 28-29 -0.4858955073185385 1102 30-31 -0.1472687131624042 1102 32-33 -5.490038967552149 1102 34-35 -0.04948316095324046 1102 36-37 -0.09346362147336507 1102 38-39 0.09547625700983886 1102 40-41 0.26773552190044825 1102 42-43 -0.16093856595277956 1102 44-45 -4.506382166586533 1102 46-47 -0.7757260908201147 1102 48-49 0.20120252756311885 1102 50-51 -4.840821856806343 1102 52-53 -1.8542058186828783 1102 54-55 -0.32313905500553375 1102 56-57 -0.9617602029610097 1102 58-59 1.0151469823596493 1102 60-61 0.045242478429447175 1102 62-63 -3.430376888889885 1102 64-65 -0.38163966151048 1102 66-67 0.3641251619613435 1102 68-69 -5.952087120699527 1102 70-71 -0.4727543532622782 1102 72-73 0.15566163934174426 1102 74-75 -5.152306018776617 1102 76 -1.6998886805438147 1103 1 -1.268830553521834 1103 2 -0.9633821743073447 1103 3 -0.6307839105683364 1103 4 -0.3918221489301672 1103 5 -0.19888522066572278 1103 6 -0.7800092259316109 1103 7 -0.8728610748336507 1103 8 0.805744058913735 1103 9 -0.46773154473073575 1103 10-11 -0.4920554378034865 1103 12-13 -1.504327697634153 1103 14-15 -0.7259110108938387 1103 16-17 -1.1967301528840295 1103 18-19 -0.7604273845887839 1103 20-21 -0.8954181975104412 1103 22-23 -1.4336672845955434 1103 24-25 -1.016286756305938 1103 26-27 -0.8424798868675296 1103 28-29 -1.0915176792642391 1103 30-31 -0.9752890751533521 1103 32-33 -4.5236928137059955 1103 34-35 -1.9439627989622856 1103 36-37 -0.9429658839168056 1103 38-39 -0.9645463674245498 1103 40-41 -0.32971922923077557 1103 42-43 -0.7303050817898864 1103 44-45 -4.550081261609158 1103 46-47 -1.2395496202318803 1103 48-49 -1.026648151169912 1103 50-51 -1.0797698206072468 1103 52-53 -1.544443375243965 1103 54-55 -0.5066684667702397 1103 56-57 -0.5462398409700597 1103 58-59 -0.9719684022557331 1103 60-61 -0.7864656663669329 1103 62-63 -0.989856526898933 1103 64-65 -0.3261871728226957 1103 66-67 -0.7140648832875236 1103 68-69 -3.25917988088052 1103 70-71 -0.2936819550722305 1103 72-73 -0.6078338357713768 1103 74-75 -1.7303263807675648 1103 76 -1.936019902263272 1104 1 -1.5859479712085118 1104 2 -0.6743107144578957 1104 3 0.20175357092550072 1104 4 -0.5837023749659913 1104 5 -0.5430268475397959 1104 6 -0.7824280594699893 1104 7 0.8456784501574894 1104 8 1.7996761230545317 1104 9 0.6322538588599791 1104 10-11 0.05936562690030911 1104 12-13 -0.04618811425737945 1104 14-15 -0.3398985413899105 1104 16-17 -1.045063034423741 1104 18-19 0.43636349057021206 1104 20-21 1.1698694560125098 1104 22-23 -0.16526016988861514 1104 24-25 0.164156140455745 1104 26-27 0.4377398933522514 1104 28-29 -0.06595311100517165 1104 30-31 -0.6745613398905128 1104 32-33 -1.1568016403632093 1104 34-35 -0.5626628844555448 1104 36-37 0.37897877483999665 1104 38-39 0.012964736272511601 1104 40-41 0.9088069504718774 1104 42-43 0.8878632773651915 1104 44-45 -2.2489052080611884 1104 46-47 0.12342137461767066 1104 48-49 0.5959721128165754 1104 50-51 0.9786366685392807 1104 52-53 0.35651998776873484 1104 54-55 0.15557062241381914 1104 56-57 0.957179889204891 1104 58-59 1.276702281898821 1104 60-61 0.5582493908718469 1104 62-63 1.0504295627230213 1104 64-65 0.2802727808858343 1104 66-67 0.6126965905327744 1104 68-69 -2.6308774432801734 1104 70-71 -0.08208615049730028 1104 72-73 0.7390602568532678 1104 74-75 -0.7613958805277683 1104 76 0.27133251761286914 1105 1 -0.46351572876533353 1105 2 0.12991485767363642 1105 3 -0.5586541460158401 1105 4 -0.4018114119104581 1105 5 0.5428319355579134 1105 6 0.09273791725421532 1105 7 0.7141663045666107 1105 8 0.4145905962248797 1105 9 0.6589077681000006 1105 10-11 0.44080942913416266 1105 12-13 -1.2863661975191114 1105 14-15 -0.08234171877021268 1105 16-17 0.6515998570860617 1105 18-19 0.6145150956627674 1105 20-21 -0.23812085875746902 1105 22-23 0.17050097984191837 1105 24-25 0.39807592488126886 1105 26-27 0.13356313790431074 1105 28-29 -0.48475143952192923 1105 30-31 -0.08426023858613263 1105 32-33 0.10847798160039446 1105 34-35 0.7052626017586228 1105 36-37 0.24831603954358172 1105 38-39 0.47157795192509155 1105 40-41 0.024727047324176965 1105 42-43 0.24041736625061105 1105 44-45 1.3674737656168574 1105 46-47 0.6561383159595451 1105 48-49 -0.2232889978606103 1105 50-51 0.16905102454958865 1105 52-53 -1.3284854796998289 1105 54-55 -0.613223800768246 1105 56-57 -0.18515003346948333 1105 58-59 -0.008963187131875827 1105 60-61 -0.19064735207902928 1105 62-63 0.3330976873813043 1105 64-65 0.2775976266251128 1105 66-67 0.2370912636562572 1105 68-69 0.45837898099538776 1105 70-71 0.8310592060597592 1105 72-73 0.41850062239259245 1105 74-75 -0.680399239115598 1105 76 -0.8798039347811013 1106 1 -0.07487247976836287 1106 2 0.2204331578455836 1106 3 -0.5326572574544706 1106 4 -0.23594307489252486 1106 5 -0.20069954728823092 1106 6 0.21111587516791275 1106 7 0.18622476686088874 1106 8 0.1433836855559214 1106 9 0.466755962646797 1106 10-11 0.38984938655658397 1106 12-13 -0.2581585499304815 1106 14-15 -0.22505849597482452 1106 16-17 0.41168132765021426 1106 18-19 0.20919903900183456 1106 20-21 -0.4578048020965362 1106 22-23 0.17915978603204152 1106 24-25 0.5310387513776256 1106 26-27 0.39460669804514126 1106 28-29 -0.3938544445166059 1106 30-31 0.04356462017093321 1106 32-33 0.2161808392111375 1106 34-35 0.7135120081288875 1106 36-37 0.4303044944686647 1106 38-39 -0.4733884772896779 1106 40-41 0.2507669228666316 1106 42-43 0.5993995982984259 1106 44-45 1.000610587036654 1106 46-47 0.5004333294697396 1106 48-49 0.12253102997857823 1106 50-51 0.7867022977830302 1106 52-53 0.016096113684270108 1106 54-55 0.21058075175775315 1106 56-57 -0.08441720779192607 1106 58-59 -0.17189408044228216 1106 60-61 -0.0011464104594409719 1106 62-63 0.03718349758354478 1106 64-65 0.041476280518505604 1106 66-67 0.8338836160676237 1106 68-69 0.86374621263381 1106 70-71 0.17954033272806313 1106 72-73 -0.07659681476453528 1106 74-75 -0.805675583994006 1106 76 -0.5761930283699002 1107 1 -0.23931778687671468 1107 2 0.8098482717172182 1107 3 1.248700575533796 1107 4 1.0980372563704144 1107 5 0.2743694658242575 1107 6 0.5922839220968328 1107 7 0.441012551539977 1107 8 0.7108224364185851 1107 9 -0.423189689527117 1107 10-11 -0.04950534084162328 1107 12-13 0.6304391898900867 1107 14-15 -0.27423033862493185 1107 16-17 0.6445250992168141 1107 18-19 1.2109890666070804 1107 20-21 0.43549445601250625 1107 22-23 -0.23136040030336602 1107 24-25 -0.6512960484843511 1107 26-27 0.05157917906653964 1107 28-29 -0.19937765017568054 1107 30-31 0.2303205725518822 1107 32-33 0.9296038895907124 1107 34-35 0.7611418390467612 1107 36-37 0.22992923566948775 1107 38-39 0.7644048284384084 1107 40-41 0.9512176647575892 1107 42-43 0.20504357690436237 1107 44-45 0.14013569055632402 1107 46-47 0.5090060520370265 1107 48-49 0.7386132418488351 1107 50-51 0.9548031431936579 1107 52-53 0.9845441813171192 1107 54-55 1.1048073735658974 1107 56-57 0.8195790827532754 1107 58-59 0.8144326966453654 1107 60-61 0.03283176414373301 1107 62-63 -0.11287688888988257 1107 64-65 1.0568424813466635 1107 66-67 0.7287680191042014 1107 68-69 1.2804128793004743 1107 70-71 0.3053706467377246 1107 72-73 0.7227152107703141 1107 74-75 0.3859975526519577 1107 76 0.13207560517047057 1108 1 -0.7274913457739594 1108 2 0.1746665674565655 1108 3 -0.6161177202055512 1108 4 0.0790836222852036 1108 5 0.15500856356862158 1108 6 0.48279144089381987 1108 7 0.567422325976068 1108 8 1.7665085266441451 1108 9 0.33174452099919804 1108 10-11 0.21177285464709783 1108 12-13 0.9880911322459802 1108 14-15 0.7890528693951175 1108 16-17 -0.4597590486528631 1108 18-19 -0.015279730385401535 1108 20-21 0.4004458971152651 1108 22-23 -0.2820339592006036 1108 24-25 -0.7680332038728217 1108 26-27 -0.2154555953695514 1108 28-29 0.5983588786463727 1108 30-31 0.8308453219253167 1108 32-33 0.8049171727987279 1108 34-35 0.08981508466079546 1108 36-37 0.5893433960704968 1108 38-39 0.8234587131501883 1108 40-41 0.14690218856711112 1108 42-43 0.8261666972051103 1108 44-45 0.41594239481697315 1108 46-47 0.5319932074254972 1108 48-49 0.5712025275631198 1108 50-51 0.39641498529891805 1108 52-53 0.34145207605396166 1108 54-55 0.8475626993804326 1108 56-57 1.2726257619512715 1108 58-59 -0.5259495088684183 1108 60-61 1.2495845836926058 1108 62-63 0.32681609356625785 1108 64-65 0.27993928585794237 1108 66-67 0.07886200406660393 1108 68-69 0.8637462126338065 1108 70-71 1.4640877520008821 1108 72-73 0.5446528674119229 1108 74-75 1.0429132794690013 1108 76 0.5826551791053092 1109 1 -0.03889485454588737 1109 2 -0.7069123799118557 1109 3 -0.6555914044160787 1109 4 -0.6972321671884814 1109 5 -0.11253529608050172 1109 6 -0.08738399770266625 1109 7 -0.8580162705151615 1109 8 1.1261576494511658 1109 9 0.9106918894202529 1109 10-11 0.895983380962889 1109 12-13 -0.22024220108735548 1109 14-15 -0.42805239376277626 1109 16-17 -0.5694081714598767 1109 18-19 -0.2959814847713709 1109 20-21 -0.014027787095262312 1109 22-23 0.14559761974676277 1109 24-25 0.3481948663026202 1109 26-27 -0.19571875326429122 1109 28-29 -0.5902376125816957 1109 30-31 -0.175733625443101 1109 32-33 0.08342594472855325 1109 34-35 -0.5154480732339408 1109 36-37 -0.2439899372628389 1109 38-39 0.1282832745536986 1109 40-41 -0.1645013202048169 1109 42-43 0.3371316094858159 1109 44-45 -0.48756637711284867 1109 46-47 -1.0688839855569583 1109 48-49 -0.33669220927898635 1109 50-51 0.7889588449480449 1109 52-53 0.08268014622940001 1109 54-55 0.10852761166113112 1109 56-57 -0.3019356415575025 1109 58-59 0.01791014025438642 1109 60-61 0.1509003731662908 1109 62-63 -0.2323944327495333 1109 64-65 -0.6696221176508281 1109 66-67 -0.3356116801439164 1109 68-69 0.13348305473907018 1109 70-71 -0.4613508444903438 1109 72-73 -1.310610290482817 1109 74-75 0.5297553847321588 1109 76 -1.3910290314210059 1110 1 0.13197245457197582 1110 2 0.04345826501031169 1110 3 -1.2442990891207977 1110 4 -0.6231649569092639 1110 5 -0.44469492049364945 1110 6 -0.5314166144558179 1110 7 -0.6153673948047569 1110 8 1.0375117389001396 1110 9 -1.282784759949653 1110 10-11 -0.687751484369457 1110 12-13 -0.603705260143446 1110 14-15 -0.4159137725618862 1110 16-17 0.3235681072651033 1110 18-19 -0.049595272762470444 1110 20-21 0.16718669276636078 1110 22-23 -0.1473354170706358 1110 24-25 0.022737737565282856 1110 26-27 -1.0790273769361463 1110 28-29 -0.2671279016847343 1110 30-31 -0.5432898399229664 1110 32-33 0.12527793385629948 1110 34-35 -0.4628164942865709 1110 36-37 -0.6297077529287662 1110 38-39 -0.9305566068399287 1110 40-41 0.027183878707958797 1110 42-43 -0.2688728382532517 1110 44-45 0.7654605564181622 1110 46-47 0.42256029415641194 1110 48-49 0.38516966371335215 1110 50-51 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0.8459017889247278 2116 40-41 0.2568312665812975 2116 42-43 0.6196997319195603 2116 44-45 2.6456923014985776 2116 46-47 0.4043802921586064 2116 48-49 0.9382238041588629 2116 50-51 1.5389121857468524 2116 52-53 1.8415920536575427 2116 54-55 0.6333503066965918 2116 56-57 0.5607929885283518 2116 58-59 -0.24426791125737068 2116 60-61 -0.9393851811450169 2116 62-63 1.3352614089824577 2116 64-65 1.3957007640214378 2116 66-67 0.7160400555783646 2116 68-69 0.09424266653451596 2116 70-71 -2.044030949006956 2116 72-73 1.0359275967885537 2116 74-75 -0.784167720904275 2116 76 -0.2380801699055155 2117 1 0.5039382921506395 2117 2 0.9183209160941175 2117 3 1.0391641013696606 2117 4 1.1027864964919942 2117 5 -0.21537217551312793 2117 6 0.36716994033391614 2117 7 -0.5983111566667105 2117 8 -1.7161342505115051 2117 9 0.7835922253665011 2117 10-11 0.010904993504880167 2117 12-13 1.091067989282184 2117 14-15 0.8355720929860091 2117 16-17 1.088626542985807 2117 18-19 1.539731467822886 2117 20-21 0.8792159605717735 2117 22-23 1.3350339765963355 2117 24-25 0.6920265199047115 2117 26-27 1.3970863979115187 2117 28-29 0.9418172586389062 2117 30-31 0.14640149960355586 2117 32-33 1.8517163515967816 2117 34-35 0.6524317326637821 2117 36-37 -0.32069766402656086 2117 38-39 1.1118592357332417 2117 40-41 -0.06497724405700112 2117 42-43 -0.24732154467618273 2117 44-45 1.100479535541126 2117 46-47 -0.09296013337330677 2117 48-49 0.06322380415886286 2117 50-51 1.0335930368106787 2117 52-53 1.6740388621681817 2117 54-55 0.06420137052638353 2117 56-57 0.1671759672517581 2117 58-59 0.18658315257241398 2117 60-61 1.6723169465145595 2117 62-63 1.0480273664292632 2117 64-65 -0.03515029980835038 2117 66-67 -0.6509812210173784 2117 68-69 0.5676469218536688 2117 70-71 1.6102243701419816 2117 72-73 1.692842490405578 2117 74-75 0.7929599386701938 2117 76 0.6023453620093768 2118 1 -0.7809466846287094 2118 2 -0.022412424439416156 2118 3 0.7954282767665717 2118 4 0.5938222454929658 2118 5 -0.09772988152889539 2118 6 0.20908051382997073 2118 7 0.49027832383874426 2118 8 -2.6455272735161515 2118 9 -0.17239654231899593 2118 10-11 -0.3628664720961581 2118 12-13 0.15555929503874566 2118 14-15 -0.30160524797953414 2118 16-17 -0.2451028014358343 2118 18-19 -0.28722068683744695 2118 20-21 -2.41569979706944 2118 22-23 -0.8703877752127198 2118 24-25 -0.554679387860709 2118 26-27 0.41816048798604655 2118 28-29 0.29825423887435676 2118 30-31 0.16454600764978267 2118 32-33 0.6118011339706904 2118 34-35 0.7030041486914307 2118 36-37 0.01729779984643187 2118 38-39 -0.21977755009675803 2118 40-41 -0.7811857978965087 2118 42-43 -0.8757101395568405 2118 44-45 0.123173670976918 2118 46-47 0.11369015283470674 2118 48-49 -0.37867056888358164 2118 50-51 0.06512991984340388 2118 52-53 -0.9934063262970945 2118 54-55 -0.11714920728979905 2118 56-57 0.6032651777496518 2118 58-59 0.950718048349497 2118 60-61 -0.5843387398446644 2118 62-63 0.09619671517102901 2118 64-65 -0.3840000676018498 2118 66-67 -0.8514078329625114 2118 68-69 1.1141692244781396 2118 70-71 -0.7733888710287715 2118 72-73 -1.2694779545668844 2118 74-75 0.1734680928985135 2118 76 0.5054666494054239 2119 1 1.5711529923440644 2119 2 2.01964264401159 2119 3 1.9222395365727571 2119 4 1.3601043559375157 2119 5 1.8162525827073743 2119 6 2.04212158443449 2119 7 -0.164236366208943 2119 8 0.9970507913969406 2119 9 0.5792724316850055 2119 10-11 1.3213821050458208 2119 12-13 0.9855392981151958 2119 14-15 0.7541246397300476 2119 16-17 1.2321468524635648 2119 18-19 0.7598797592477702 2119 20-21 -0.10242491628186556 2119 22-23 1.8840346213416552 2119 24-25 1.19695882164552 2119 26-27 1.2109000665124192 2119 28-29 2.0670590381360086 2119 30-31 1.5526555292618411 2119 32-33 1.831551941538759 2119 34-35 2.1129410814710035 2119 36-37 0.9725969845872413 2119 38-39 1.6001732267068078 2119 40-41 1.0741749158398406 2119 42-43 0.7211826461684296 2119 44-45 0.30126934856498266 2119 46-47 1.0980617879677652 2119 48-49 1.2310510124116014 2119 50-51 2.3622842037997174 2119 52-53 0.1320820601049988 2119 54-55 0.2289821571156807 2119 56-57 2.8743004030995998 2119 58-59 2.6459803156929844 2119 60-61 1.9362273269142953 2119 62-63 1.233714020201024 2119 64-65 2.0824512475804333 2119 66-67 2.032610010446195 2119 68-69 1.1455643944519913 2119 70-71 0.4537607982528762 2119 72-73 -0.1207777545976505 2119 74-75 1.0915576175870214 2119 76 2.269656774001639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 16.0 13 137.0 14 600.0 15 1204.0 16 2126.0 17 2557.0 18 2846.0 19 2689.0 20 2359.0 21 1771.0 22 1317.0 23 1064.0 24 997.0 25 957.0 26 964.0 27 1067.0 28 1304.0 29 1489.0 30 1832.0 31 2266.0 32 2888.0 33 3885.0 34 5141.0 35 7613.0 36 12818.0 37 15170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.013122124634044 28.524937264742782 10.062473860309494 28.399466750313678 2 17.03662381131537 23.370220936417535 29.898418546723576 29.694736705543516 3 15.657555039503897 24.69739624550797 27.54245533918865 32.10259337579948 4 11.471049934484503 19.58978217719023 32.43211686407805 36.507051024247225 5 10.81978697733553 38.75274062349995 31.041372063154345 19.38610033601017 6 24.658476148467194 35.9738456947886 17.661940037103825 21.705738119640376 7 21.687575407688016 30.644387073338436 19.354964258377553 28.313073260595996 8 18.74651340797343 38.952530454975935 18.41309790999079 23.887858227059848 9 20.674355548059832 22.149427225905217 19.175931811989987 38.00028541404497 10-11 20.779439810069928 27.44126308688263 25.55363838040503 26.225658722642414 12-13 20.59392068084223 24.638367431662797 25.180005448813585 29.587706438681387 14-15 20.100284116708398 26.374203759681375 22.546412215721123 30.9790999078891 16-17 21.177073468169848 28.071119990659177 23.921588977828517 26.830217563342458 18-19 19.68513641494013 26.976816595529378 24.475551692375554 28.86249529715494 20-21 19.12403834926895 27.526887300372337 24.917943462072365 28.431130888286347 22-23 22.07223570010768 27.153254368780892 24.127865492144625 26.6466444389668 24-25 20.286451914220105 29.33667181276839 23.949481714041074 26.427394558970434 26-27 18.856138347971616 30.069666973702986 25.350605207508988 25.723589470816417 28-29 20.589380002854142 27.7558672046289 27.25444662108691 24.400306171430053 30-31 20.387644166526123 27.39261296558166 27.46785848652716 24.75188438136506 32-33 18.672565223595956 30.430975207898186 25.98694879412566 24.9095107743802 34-35 19.619620918254824 28.67567883135922 26.31582361412021 25.388876636265746 36-37 19.97898314759798 27.71435243445207 26.677131848315412 25.629532569634538 38-39 19.71627249257275 26.592156303109714 28.15220352616079 25.539367678156744 40-41 18.998845370454458 26.437773251514642 27.485372530195505 27.078008847835395 42-43 17.689184105032368 25.907811263476084 28.528431130888286 27.874573500603262 44-45 17.668426719943955 26.559074220625057 28.97471491028918 26.797784149141812 46-47 17.853297180887637 26.860704972691064 28.335127982252434 26.950869864168865 48-49 17.21630492598695 28.035443235038464 27.489264539899587 27.258987299075 50-51 17.037921147883395 27.490561876467613 27.217472528898174 28.25404444675082 52-53 16.818671267887026 27.397802311853763 27.282339357299463 28.501187062959744 54-55 16.6415848263515 27.825274711018277 26.535073494116578 28.99806696851364 56-57 17.497826961248556 27.880411515159377 26.26198414654714 28.359777377044924 58-59 17.232521633087273 26.952167200736888 26.43452991009458 29.380781256081267 60-61 16.803103229070718 26.361879062285126 26.787405456597607 30.047612252046545 62-63 17.425824781723122 26.989789961209638 25.92662264371246 29.657762613354784 64-65 18.058925026919734 27.491210544751628 24.49047106290785 29.959393365420787 66-67 17.168952141254003 29.134935976440367 23.66860834706348 30.02750353524215 68-69 17.279225749536202 29.289319028035443 23.30665144458427 30.124803777844082 70-71 17.25133301332365 29.97755607737315 23.425357740558635 29.345753168744565 72-73 17.3894993578184 30.2610241174868 23.206756528846277 29.14271999584852 74-75 18.226281444195067 30.86493428990283 22.54576354743711 28.363020718464995 76 18.999494038738472 29.67527665702313 23.045886794411075 28.279342509827327 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 1.0 11 2.0 12 2.0 13 3.5 14 7.0 15 9.5 16 11.5 17 13.0 18 18.5 19 29.0 20 37.0 21 40.0 22 43.5 23 64.5 24 99.5 25 117.0 26 146.5 27 219.0 28 335.0 29 408.0 30 465.5 31 626.5 32 894.5 33 1059.0 34 1197.0 35 1521.5 36 1923.0 37 2138.0 38 2434.5 39 3113.0 40 3806.5 41 4328.5 42 4539.0 43 4709.5 44 5082.0 45 5444.0 46 5604.0 47 5562.0 48 5418.5 49 5089.0 50 4861.0 51 4550.5 52 3872.5 53 3284.0 54 3063.0 55 2894.0 56 2378.5 57 1816.0 58 1600.0 59 1418.0 60 1049.5 61 780.0 62 697.0 63 620.5 64 486.5 65 360.5 66 246.5 67 201.0 68 178.5 69 132.5 70 86.5 71 64.0 72 49.5 73 35.0 74 31.0 75 27.0 76 22.5 77 19.0 78 18.5 79 17.0 80 16.5 81 12.5 82 9.0 83 9.0 84 9.0 85 7.0 86 6.0 87 7.0 88 4.5 89 4.5 90 5.5 91 4.0 92 4.0 93 4.0 94 3.0 95 3.0 96 4.0 97 3.0 98 4.5 99 7.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7381845072067047 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 77081.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.66073351409557 #Duplication Level Percentage of deduplicated Percentage of total 1 82.240296698858 58.111596891581584 2 8.954209965850255 12.654220884524072 3 3.9859729005250983 8.449553067552316 4 1.8304997613189882 5.173778233287061 5 1.009804281570154 3.5676755620710683 6 0.6242426467888224 2.6465665987727194 7 0.3965776814893695 1.9615728908550745 8 0.26254911320824 1.4841530338215643 9 0.1817647706826277 1.155926882111026 >10 0.5140821797084419 4.794955955423515 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003892009704077529 0.0 2 0.0 0.0 0.0 0.005189346272103372 0.0 3 0.0 0.0 0.0 0.005189346272103372 0.0 4 0.0 0.0 0.0 0.009081355976180901 0.0 5 0.0 0.0 0.0 0.009081355976180901 0.0 6 0.0 0.0 0.0 0.009081355976180901 0.0 7 0.0 0.0 0.0 0.009081355976180901 0.0 8 0.0 0.0 0.0 0.009081355976180901 0.0 9 0.0 0.0 0.0 0.010378692544206744 0.0 10 0.0 0.0 0.0 0.011676029112232587 0.0 11 0.0 0.0 0.0 0.011676029112232587 0.0 12 0.0 0.0 0.0 0.014270702248284273 0.0 13 0.0 0.0 0.0 0.015568038816310116 0.0 14 0.0 0.0 0.0 0.018162711952361802 0.0 15 0.0 0.0 0.0 0.019460048520387643 0.0 16 0.0 0.0 0.0 0.019460048520387643 0.0 17 0.0 0.0 0.0 0.024649394792491015 0.0 18 0.0 0.0 0.0 0.033730750768671915 0.0 19 0.0 0.0 0.0 0.04410944331287866 0.0 20 0.0 0.0 0.0 0.0454067798809045 0.0 21 0.0 0.0 0.0 0.0454067798809045 0.0 22 0.0 0.0 0.0 0.048001453016956186 0.0 23 0.0 0.0 0.0 0.048001453016956186 0.0 24 0.0 0.0 0.0 0.0544881358570854 0.0 25 0.0 0.0 0.0 0.05708280899313709 0.0 26 0.0 0.0 0.0 0.06616416496931798 0.0 27 0.0 0.0 0.0 0.09211089632983485 0.0 28 0.0 0.0 0.0 0.12454431053048093 0.0 29 0.0 0.0 0.0 0.17254576354743711 0.0 30 0.0 0.0 0.0 0.2049791777480832 0.0 31 0.0 0.0 0.0 0.23092590910860003 0.0 32 0.0 0.0 0.0 0.2763326889895045 0.0 33 0.0 0.0 0.0 0.338604844254745 0.0 34 0.0 0.0 0.0 0.39439031667985625 0.0 35 0.0 0.0 0.0 0.5020692518260013 0.0 36 0.0 0.0 0.0 0.6823990347815934 0.0 37 0.0 0.0 0.0 0.9639210700432013 0.0 38 0.0 0.0 0.0 1.3180939531142564 0.0 39 0.0 0.0 0.0 1.80070315641987 0.0 40 0.0 0.0 0.0 2.2690416574771994 0.0 41 0.0 0.0 0.0 2.922899287762224 0.0 42 0.0 0.0 0.0 3.7025985651457556 0.0 43 0.0 0.0 0.0 4.57181406572307 0.0 44 0.0 0.0 0.0 5.215293003463889 0.0 45 0.0 0.0 0.0 5.831527873276164 0.0 46 0.0 0.0 0.0 6.577496399891023 0.0 47 0.0 0.0 0.0 7.245624732424333 0.0 48 0.0 0.0 0.0 7.800884783539393 0.0 49 0.0 0.0 0.0 8.353550161518402 0.0 50 0.0 0.0 0.0 8.882863481272947 0.0 51 0.0 0.0 0.0 9.479638302564835 0.0 52 0.0 0.0 0.0 10.237282858291927 0.0 53 0.0 0.0 0.0 11.04811821330808 0.0 54 0.0 0.0 0.0 11.805762769035171 0.0 55 0.0 0.0 0.0 12.462215072456248 0.0 56 0.0 0.0 0.0 13.065476576588264 0.0 57 0.0 0.0 0.0 13.663548734448177 0.0 58 0.0 0.0 0.0 14.186375371362592 0.0 59 0.0 0.0 0.0 14.64303784330769 0.0 60 0.0 0.0 0.0 15.163269807086053 0.0 61 0.0 0.0 0.0 15.674420414888235 0.0 62 0.0 0.0 0.0 16.198544388370674 0.0 63 0.0 0.0 0.0 16.760291122325864 0.0 64 0.0 0.0 0.0 17.310361827168823 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTATC 15 0.0022179103 69.99286 34 GCAAGAT 15 0.0022179103 69.99286 23 GTGTCAA 15 0.0022179103 69.99286 50 CATAAGT 15 0.0022179103 69.99286 14 TTTTAGA 15 0.0022179103 69.99286 28 AAAGAGG 15 0.0022179103 69.99286 30 TCAATAC 25 2.3782131E-4 55.99429 3 GTCATAA 20 0.006930364 52.494648 12 TGGAGTC 20 0.006930364 52.494648 20 TACTTGG 20 0.006930364 52.494648 7 GTTGTAA 20 0.006930364 52.494648 70 GTCCGTA 20 0.006930364 52.494648 24 GCTAGTC 20 0.006930364 52.494648 65 TCCGTAA 20 0.006930364 52.494648 25 AGCTAGT 20 0.006930364 52.494648 64 GCATGGT 20 0.006930364 52.494648 16 CCCATGA 20 0.006930364 52.494648 34 GAAGGTC 20 0.006930364 52.494648 36 ATTGAGG 30 5.644912E-4 46.997383 1 TACGGAT 30 5.8480696E-4 46.661907 7 >>END_MODULE