FastQCFastQC Report
Wed 25 May 2016
SRR1780408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77081
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT41785.420272181211972No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT40305.228266369144148No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC14751.9135714378381181No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC7330.9509477043629428No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG3970.5150426175062597No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT3330.4320130771526057No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1930.2503859576289877No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1720.22314188970044496No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1630.21146586058821243No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT1620.21016852402018657No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT1200.15568038816310115No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1180.15308571502704946No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT1160.15049104189099777No Hit
CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC1140.1478963687549461No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT1090.14140968591481687No Hit
TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC1020.132328329938636No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1010.13103099337061014No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT870.11286828141824834No Hit
CTTATCACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC810.10508426201009329No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG800.10378692544206743No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGTCTT780.10119225230601574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAG150.002217013470.030
CCAGAAG150.002217013470.060
CCGACCC150.002217013470.09
GCCGTTG150.002217013470.028
TTTACAC2050.063.170732
CTTTACA2250.057.5555571
TTAATAC252.3770155E-455.9999962
ATATCCG252.3770155E-455.99999610
TATCCGA252.3770155E-455.99999611
CTATACA1200.055.4166641
TCAAAAT200.006927568552.50000446
AGCCGTT200.006927568552.50000427
AGTCATC200.006927568552.50000466
GTACACT200.006927568552.50000441
ACCATAT200.006927568552.50000440
CGGCAGG200.006927568552.50000470
GCCGGGA200.006927568552.50000444
TTCGTCC200.006927568552.50000420
TTTCCAG200.006927568552.50000435
TAGCCGT200.006927568552.50000426
Produced by FastQC (version 0.11.5)