##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780408_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 77081 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86132769424372 34.0 34.0 34.0 31.0 34.0 2 33.25723589470817 34.0 34.0 34.0 32.0 34.0 3 33.47564250593531 34.0 34.0 34.0 34.0 34.0 4 33.52055629792037 34.0 34.0 34.0 34.0 34.0 5 33.532219353666925 34.0 34.0 34.0 34.0 34.0 6 37.26364473735421 38.0 37.0 38.0 36.0 38.0 7 36.91899430469247 38.0 37.0 38.0 35.0 38.0 8 36.91146975259792 38.0 37.0 38.0 35.0 38.0 9 36.694126957356545 38.0 37.0 38.0 34.0 38.0 10-11 36.41342873081564 38.0 37.0 38.0 34.0 38.0 12-13 36.27103306910912 38.0 37.0 38.0 34.0 38.0 14-15 36.44981253486592 38.0 37.0 38.0 34.0 38.0 16-17 36.55236699056836 38.0 37.0 38.0 34.0 38.0 18-19 36.584151736485 38.0 37.0 38.0 34.0 38.0 20-21 36.368430611953656 38.0 37.0 38.0 34.0 38.0 22-23 36.38188399216409 38.0 37.0 38.0 33.5 38.0 24-25 35.59493909004813 38.0 36.0 38.0 31.0 38.0 26-27 35.821765415601774 38.0 36.0 38.0 31.5 38.0 28-29 35.74389278810602 38.0 36.5 38.0 31.5 38.0 30-31 35.41746993422504 38.0 36.0 38.0 29.0 38.0 32-33 35.849158677235636 38.0 36.5 38.0 31.5 38.0 34-35 35.631485061169414 38.0 36.0 38.0 31.0 38.0 36-37 35.6788443325852 38.0 36.0 38.0 31.5 38.0 38-39 35.117590586525864 38.0 35.5 38.0 29.0 38.0 40-41 35.09312281885289 37.0 35.5 38.0 29.0 38.0 42-43 35.706406247972915 38.0 36.5 38.0 32.5 38.0 44-45 35.92317821512435 38.0 37.0 38.0 32.5 38.0 46-47 36.15645230342108 38.0 37.0 38.0 34.0 38.0 48-49 35.88212399942917 38.0 36.5 38.0 32.0 38.0 50-51 35.97662848172702 38.0 37.0 38.0 32.5 38.0 52-53 35.849002996847474 38.0 37.0 38.0 32.0 38.0 54-55 36.086097741337035 38.0 37.0 38.0 34.0 38.0 56-57 36.086966956837614 38.0 37.0 38.0 33.5 38.0 58-59 36.06101373879426 38.0 37.0 38.0 34.0 38.0 60-61 35.83063271104423 38.0 37.0 38.0 32.5 38.0 62-63 36.198966022755286 38.0 37.0 38.0 34.0 38.0 64-65 36.2018136765221 38.0 37.0 38.0 34.0 38.0 66-67 35.238846148856396 38.0 36.0 38.0 29.0 38.0 68-69 33.79186180770877 37.0 34.0 38.0 22.5 38.0 70-71 32.85368638185805 37.0 32.5 38.0 16.0 38.0 72-73 31.895246558814755 37.0 29.0 38.0 11.0 38.0 74-75 32.25859161142175 37.0 31.0 38.0 11.0 38.0 76 31.859576289876884 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2489526634814112 1101 2 0.45155572527009014 1101 3 0.010748181122515632 1101 4 0.26644235639518143 1101 5 0.21883622189235297 1101 6 -0.9613524847829495 1101 7 0.13952819541441386 1101 8 0.5189091358128053 1101 9 0.35255055609736985 1101 10-11 0.5506658492487517 1101 12-13 0.6497347731365366 1101 14-15 0.5610575265615125 1101 16-17 0.037501084665279905 1101 18-19 -0.6058152802027621 1101 20-21 0.08038145793702256 1101 22-23 0.7359462415356361 1101 24-25 -1.875961006959983 1101 26-27 -0.7534092825784313 1101 28-29 0.6771936709838116 1101 30-31 1.086989079664015 1101 32-33 0.13220457722592727 1101 34-35 0.42020858248923076 1101 36-37 0.3247566634044148 1101 38-39 0.10565704040713086 1101 40-41 1.6323837255698521 1101 42-43 0.8858223971170318 1101 44-45 0.5411026071845129 1101 46-47 0.5556120446921966 1101 48-49 -1.6489305038171977 1101 50-51 -1.1958256316280185 1101 52-53 -1.9816939296268288 1101 54-55 -0.8345401297142914 1101 56-57 -0.9838606233504166 1101 58-59 -1.576556987504972 1101 60-61 -1.6733471527404475 1101 62-63 -0.9221298028080298 1101 64-65 -0.9984487574746126 1101 66-67 -1.6713445298792777 1101 68-69 -1.7143817888976542 1101 70-71 -1.3497091415845865 1101 72-73 -1.866176463126287 1101 74-75 -1.1882079010670061 1101 76 -1.0589765134316345 1102 1 -0.6202365257077744 1102 2 0.2784476171619801 1102 3 0.3896671000414358 1102 4 0.34090181585463597 1102 5 0.209511897568035 1102 6 0.319728596298134 1102 7 0.29790657379279395 1102 8 0.5226929195965937 1102 9 0.3868748804216935 1102 10-11 0.6849901735730768 1102 12-13 0.5396672055689677 1102 14-15 -0.2652938247898433 1102 16-17 0.31209567925986903 1102 18-19 -0.07142338831086192 1102 20-21 0.5722733498289116 1102 22-23 0.30324353883293753 1102 24-25 -4.922582628581605 1102 26-27 -1.348206579875736 1102 28-29 0.594626103416239 1102 30-31 1.8421917823667187 1102 32-33 0.732609982631331 1102 34-35 0.9927085824892288 1102 36-37 -2.4291622555144983 1102 38-39 0.0976840674341588 1102 40-41 1.4808296715157994 1102 42-43 0.9349440187386548 1102 44-45 1.0812377423196438 1102 46-47 1.1670309636111185 1102 48-49 -2.8935250984117857 1102 50-51 -0.3745418478442346 1102 52-53 0.053170935238036066 1102 54-55 0.5158652756911053 1102 56-57 1.0801934307036376 1102 58-59 0.05465922871123752 1102 60-61 -5.50327958517288 1102 62-63 -2.0346973703755964 1102 64-65 0.19648367495781116 1102 66-67 1.5266960106612615 1102 68-69 -0.8888412483571102 1102 70-71 -1.465452384827831 1102 72-73 -5.404757544207371 1102 74-75 -1.6109781713372762 1102 76 0.0733207838656611 1103 1 0.6841978634324946 1103 2 0.630325445216279 1103 3 0.19482547108216153 1103 4 0.47612806019852627 1103 5 0.40401416001147084 1103 6 0.26271502163749716 1103 7 0.7468658498108951 1103 8 0.6683037793251003 1103 9 0.5528929799692079 1103 10-11 0.1383385898626699 1103 12-13 -0.6255251021233406 1103 14-15 -0.25952459402061834 1103 16-17 0.06784228559471472 1103 18-19 0.5376603220963716 1103 20-21 0.013902309104935284 1103 22-23 0.5540580184709469 1103 24-25 -3.1971301398938223 1103 26-27 -0.6968604260295805 1103 28-29 -0.11842819522629355 1103 30-31 0.9403705153983921 1103 32-33 1.0946235572919676 1103 34-35 -0.10513078402660625 1103 36-37 -1.0516283188629174 1103 38-39 -0.5967276972717244 1103 40-41 -0.006285713099586587 1103 42-43 -0.17445643375003073 1103 44-45 1.0920748463920518 1103 46-47 0.702585262253649 1103 48-49 -2.3570997590452762 1103 50-51 -0.6025395854007982 1103 52-53 -0.04578834078006366 1103 54-55 0.18534491370015616 1103 56-57 0.04666401893892669 1103 58-59 -0.66215072603989 1103 60-61 -4.738358770693246 1103 62-63 -1.3865299495611154 1103 64-65 -0.10945523906934085 1103 66-67 -0.36655059567357995 1103 68-69 -0.33945210808562365 1103 70-71 -0.6694229730631207 1103 72-73 -4.592053924297865 1103 74-75 -2.788794913418723 1103 76 -3.151724465003113 1104 1 0.09662983373922884 1104 2 -0.10908694504999517 1104 3 0.28542746870503066 1104 4 -0.13769265419144716 1104 5 -0.14604109782367658 1104 6 0.2128161539018194 1104 7 0.45431210374670883 1104 8 0.5819555924076525 1104 9 0.8943864011590179 1104 10-11 -0.6116457711273853 1104 12-13 0.26511651432472405 1104 14-15 0.7332084793576215 1104 16-17 0.14177309861470633 1104 18-19 0.7224821416430558 1104 20-21 0.06167427148790239 1104 22-23 0.6194186540403095 1104 24-25 0.8217722101280742 1104 26-27 0.9327035583731131 1104 28-29 1.3110085918955008 1104 30-31 0.9468346395095821 1104 32-33 0.7965961577465421 1104 34-35 -0.05767160184257136 1104 36-37 1.2436257629186827 1104 38-39 1.580149505222174 1104 40-41 1.7446891185204123 1104 42-43 0.7452320371718386 1104 44-45 0.5393713828726447 1104 46-47 0.7777567700627301 1104 48-49 0.37306476333936445 1104 50-51 0.2690641429391718 1104 52-53 0.5578253131182223 1104 54-55 0.4330772572579278 1104 56-57 0.24576946756999973 1104 58-59 -0.6640274072334549 1104 60-61 0.6057411521543088 1104 62-63 0.15362060197463734 1104 64-65 0.5597901265707179 1104 66-67 0.1977674392326918 1104 68-69 1.6639467700760662 1104 70-71 1.292761900886454 1104 72-73 0.9674889995714366 1104 74-75 0.3479734415659479 1104 76 0.16735304193018408 1105 1 0.8224753386990074 1105 2 0.3875154137721495 1105 3 0.4364467610583844 1105 4 0.24624079890548245 1105 5 -0.208877932940446 1105 6 0.041762494603219125 1105 7 0.7958726754877077 1105 8 -0.4481545380305292 1105 9 0.6031460668623723 1105 10-11 0.5813461057764684 1105 12-13 -1.3347819469734077 1105 14-15 0.19402820910845975 1105 16-17 0.17573974705648254 1105 18-19 0.49624610321455975 1105 20-21 0.11930724813399252 1105 22-23 0.04637913205327493 1105 24-25 0.8007224561641593 1105 26-27 0.46920867436155334 1105 28-29 0.5973379678230231 1105 30-31 1.3820646637226517 1105 32-33 0.605830321614377 1105 34-35 -0.5992829429345008 1105 36-37 0.5621936766888922 1105 38-39 -0.03765491561669165 1105 40-41 0.4736686545666515 1105 42-43 0.35405418823018664 1105 44-45 0.8304750304552471 1105 46-47 0.9041919805602703 1105 48-49 -0.34776238654738023 1105 50-51 0.5917717114777972 1105 52-53 0.2844421216787154 1105 54-55 0.9979839197589015 1105 56-57 0.622312074771429 1105 58-59 0.46720160159259905 1105 60-61 0.9925254995728778 1105 62-63 -0.39944313308745905 1105 64-65 0.9674582512289973 1105 66-67 0.7035180445595657 1105 68-69 -0.18273955344186277 1105 70-71 0.8968781236467436 1105 72-73 0.8917255066400926 1105 74-75 -0.4521222391338853 1105 76 0.3179818008148203 1106 1 -0.8774345933406238 1106 2 0.04296452537454343 1106 3 0.399232317432741 1106 4 0.28435592213482863 1106 5 0.2626278395970161 1106 6 0.004752750887504931 1106 7 -0.8096296580912608 1106 8 0.4167025814323395 1106 9 -0.46225555436091526 1106 10-11 0.6281302701914342 1106 12-13 0.9599449833467446 1106 14-15 -0.3719363368671367 1106 16-17 0.27025993046760277 1106 18-19 0.34501380975676454 1106 20-21 0.5877322870269808 1106 22-23 0.6090889494609542 1106 24-25 -0.4019304546685589 1106 26-27 1.038182309013152 1106 28-29 0.9632492918220379 1106 30-31 0.8167449224633359 1106 32-33 0.272875683114421 1106 34-35 1.126971867513383 1106 36-37 1.3179150391714956 1106 38-39 0.19838455052595094 1106 40-41 1.1995857101631486 1106 42-43 1.2446420863715062 1106 44-45 1.1390396746867992 1106 46-47 1.1933715433212555 1106 48-49 1.5622720030374921 1106 50-51 0.22708858693837186 1106 52-53 1.0251999207452869 1106 54-55 0.19695223221284408 1106 56-57 0.3218842519596734 1106 58-59 1.1642969098706644 1106 60-61 0.9541237964696307 1106 62-63 0.9600610837306789 1106 64-65 -0.7747119772161 1106 66-67 0.5469737884390398 1106 68-69 1.7020041622709101 1106 70-71 0.596419595848495 1106 72-73 0.14694535434335165 1106 74-75 -0.528526480516021 1106 76 0.2074029094695291 1107 1 0.7963706171493712 1107 2 -0.1717309542665859 1107 3 0.10109567147000575 1107 4 0.35625895871178415 1107 5 -0.2063809595748225 1107 6 0.21526431058384787 1107 7 0.6714780023642248 1107 8 -0.2856999375462621 1107 9 -0.45973226243545184 1107 10-11 0.27141874500164676 1107 12-13 -0.37586850871674216 1107 14-15 -0.06172239621841413 1107 16-17 0.7245063935455889 1107 18-19 0.8235766116891341 1107 20-21 0.6544162069717743 1107 22-23 -0.10782788973849478 1107 24-25 0.3220602285612557 1107 26-27 -0.36856372273287263 1107 28-29 0.475251103416241 1107 30-31 0.2883524966524291 1107 32-33 1.0237706969170404 1107 34-35 -0.17086284608220126 1107 36-37 1.1428913159140706 1107 38-39 0.43714835314844436 1107 40-41 0.6327939572300849 1107 42-43 0.9860154473100877 1107 44-45 0.9110591708910789 1107 46-47 0.8767631064682533 1107 48-49 1.069421330159642 1107 50-51 1.075011723584332 1107 52-53 0.944778078095176 1107 54-55 0.6208652756911093 1107 56-57 1.1068005735607755 1107 58-59 0.8771592287112426 1107 60-61 1.2102918433985508 1107 62-63 1.1004812010529719 1107 64-65 0.6107693892435222 1107 66-67 -0.0388397036244541 1107 68-69 1.1011587516428847 1107 70-71 0.12758332945788453 1107 72-73 0.41658174150691707 1107 74-75 -0.6236567427658457 1107 76 0.4579636410085186 1108 1 -0.6419909116726856 1108 2 0.45397393295145605 1108 3 -0.005420619256810255 1108 4 0.3680070790125356 1108 5 0.05591540633996317 1108 6 -0.6574643861580043 1108 7 -0.035602198137027585 1108 8 -0.2920439225086682 1108 9 -0.3020724879993608 1108 10-11 0.5113936823450089 1108 12-13 1.0681321178496646 1108 14-15 0.3649693331048951 1108 16-17 0.07463953890899688 1108 18-19 0.42335731344351757 1108 20-21 0.9113084375482146 1108 22-23 -0.09263365414951608 1108 24-25 0.9769787749271615 1108 26-27 -0.346320614963453 1108 28-29 -0.7223914404434097 1108 30-31 0.6182005542965427 1108 32-33 0.9884871756137841 1108 34-35 0.5197699859980034 1108 36-37 1.2707500251872546 1108 38-39 1.2594384533990777 1108 40-41 1.1875401978315878 1108 42-43 -0.20132791108590453 1108 44-45 0.9380798475828058 1108 46-47 0.637688858347957 1108 48-49 1.0534047261496156 1108 50-51 0.6326949942610227 1108 52-53 -0.015513275288277839 1108 54-55 -0.297994373431699 1108 56-57 0.43598290438784915 1108 58-59 0.7488697550270302 1108 60-61 1.1929046253534352 1108 62-63 0.799337717343704 1108 64-65 0.2923170082911497 1108 66-67 1.0674416246963503 1108 68-69 1.7590534884849944 1108 70-71 -0.12672431465239242 1108 72-73 0.4080056136873722 1108 74-75 -0.28961852221446804 1108 76 0.626040082111281 1109 1 -0.4402365257077747 1109 2 0.3662546347058466 1109 3 0.44633376670810065 1109 4 0.38993690357393973 1109 5 0.1260908449364493 1109 6 -0.17062228089484677 1109 7 0.003871486073492747 1109 8 0.501815726614133 1109 9 0.7198573365620433 1109 10-11 0.4763059630467623 1109 12-13 0.607605802060192 1109 14-15 0.5842675787189222 1109 16-17 0.7171833985581202 1109 18-19 0.7698485415136957 1109 20-21 1.1064838761447078 1109 22-23 0.6156996791838125 1109 24-25 -0.15898613735353706 1109 26-27 0.2238548236330331 1109 28-29 -0.2859879316714782 1109 30-31 0.8111830104368991 1109 32-33 1.3283994563155375 1109 34-35 0.05924367020853083 1109 36-37 1.0931184462398846 1109 38-39 1.0883858218201254 1109 40-41 0.9156103732701837 1109 42-43 1.0464791064579586 1109 44-45 1.0718517774073675 1109 46-47 1.1311099109795393 1109 48-49 0.8231415682548828 1109 50-51 -0.18090149696704572 1109 52-53 0.041504268571372904 1109 54-55 0.055075802006903984 1109 56-57 0.6311583429843353 1109 58-59 0.3760627374831742 1109 60-61 1.1600098885113326 1109 62-63 0.5208289454138821 1109 64-65 1.0971415696946565 1109 66-67 0.773581975573542 1109 68-69 -0.4295430027430811 1109 70-71 -0.34602256026642664 1109 72-73 -0.7191873687687718 1109 74-75 -0.5725132590565742 1109 76 -0.42220553192380805 1110 1 -1.1655886383838308 1110 2 -0.4443458100680644 1110 3 -0.13699956662522794 1110 4 0.20195815388280636 1110 5 0.33958232010323997 1110 6 0.11878962916198077 1110 7 0.7094558695674422 1110 8 0.9102046566857922 1110 9 0.9341518757268616 1110 10-11 -0.3358079484926506 1110 12-13 0.7361578158976059 1110 14-15 0.8946827010317548 1110 16-17 0.5189501393537697 1110 18-19 -0.16087174511837787 1110 20-21 0.857719359218585 1110 22-23 -0.6578362733736398 1110 24-25 -0.9050943656708057 1110 26-27 1.0765234670726187 1110 28-29 0.4068092020077927 1110 30-31 -0.36101244298539115 1110 32-33 0.5366710154951804 1110 34-35 0.5784245449305416 1110 36-37 1.1250865707765811 1110 38-39 1.0735291378566956 1110 40-41 -0.5911538965593195 1110 42-43 0.4647210140438247 1110 44-45 0.8837494794088485 1110 46-47 0.7688971607942108 1110 48-49 1.3700899250623877 1110 50-51 -0.3031216600508131 1110 52-53 0.5902131887591651 1110 54-55 -0.16904082759527483 1110 56-57 0.23404319596185275 1110 58-59 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44-45 -0.10993247044631005 2116 46-47 -0.6121711640484548 2116 48-49 -0.07490807713519132 2116 50-51 -1.3178929116740221 2116 52-53 -0.049807788166212674 2116 54-55 -0.7248794051599603 2116 56-57 -0.46757252674316874 2116 58-59 -2.547042898948334 2116 60-61 -2.14780086176863 2116 62-63 0.36083454451802055 2116 64-65 -2.2564418569570748 2116 66-67 -0.6962295212536276 2116 68-69 -0.6488412483571153 2116 70-71 1.4937497428317386 2116 72-73 0.741572243026674 2116 74-75 0.5202452329180431 2116 76 -1.3925302799641237 2117 1 0.49061453812200995 2117 2 0.4349369788641084 2117 3 0.14668837663717937 2117 4 -0.7081407373368478 2117 5 0.22323530182335105 2117 6 0.2037711494896186 2117 7 0.48492785038853725 2117 8 0.5016290898093558 2117 9 -1.0582315025570281 2117 10-11 -0.3984140817460755 2117 12-13 -1.1236838582608186 2117 14-15 0.0788551113803635 2117 16-17 -0.597159639889064 2117 18-19 -1.105944664906609 2117 20-21 0.041954200892746485 2117 22-23 0.4417541771308109 2117 24-25 -2.201306032836925 2117 26-27 -1.9464512607267963 2117 28-29 -0.7318632582858839 2117 30-31 -0.29594651550561935 2117 32-33 -1.763985762049515 2117 34-35 0.44520858248922934 2117 36-37 -1.2659707661527975 2117 38-39 0.3635351312639514 2117 40-41 0.048542437473244604 2117 42-43 0.025529125121636298 2117 44-45 -1.035464385339921 2117 46-47 -1.2185541427718647 2117 48-49 -0.6148016941564691 2117 50-51 -0.17959503933359855 2117 52-53 0.05125604162101638 2117 54-55 -0.6903049370748491 2117 56-57 0.4287040690015118 2117 58-59 -1.858213111714285 2117 60-61 -0.48024767027926174 2117 62-63 -0.9317186469713405 2117 64-65 -1.5117610058932556 2117 66-67 -1.2627188829557596 2117 68-69 -0.4041603972932819 2117 70-71 -0.511569406104428 2117 72-73 1.0474233068564658 2117 74-75 1.169181403130807 2117 76 0.9638526987592826 2118 1 -0.29556647494635513 2118 2 0.23583340396401553 2118 3 -0.8205867070651607 2118 4 0.11981044529625962 2118 5 0.23562864883706425 2118 6 0.2730280886839225 2118 7 0.8285664722699551 2118 8 0.8285812444696887 2118 9 -0.9870845104412496 2118 10-11 -0.9427763238888502 2118 12-13 -0.6071728959538731 2118 14-15 0.6619904391695428 2118 16-17 -2.473995691298498 2118 18-19 -0.4184919162296481 2118 20-21 -0.27188908671931244 2118 22-23 0.7423805946705002 2118 24-25 0.8922650871544349 2118 26-27 -1.8583207930737018 2118 28-29 -0.42489166308122606 2118 30-31 -0.1539630399683034 2118 32-33 -0.017212352394054165 2118 34-35 -0.022431011419399738 2118 36-37 -2.7271571793723695 2118 38-39 -0.38457481581457387 2118 40-41 -0.8704012929512004 2118 42-43 0.0967841202614963 2118 44-45 -2.0379754556498995 2118 46-47 0.2120944153877602 2118 48-49 0.6234799777303408 2118 50-51 -1.0247322031741888 2118 52-53 -1.8279965774523177 2118 54-55 -0.5656829476591483 2118 56-57 0.22806145100820174 2118 58-59 -0.547650415958806 2118 60-61 -0.9159318694368324 2118 62-63 -1.0399511774821946 2118 64-65 0.12332377648065318 2118 66-67 -0.45415424314585096 2118 68-69 -1.7154656138393491 2118 70-71 -0.5792468010714842 2118 72-73 0.49944296340684247 2118 74-75 0.04405989972871538 2118 76 0.2007573320890117 2119 1 0.6112786258073797 2119 2 -0.35352207980771055 2119 3 0.3296671000414335 2119 4 -0.8862193962665756 2119 5 -0.2509426478865109 2119 6 -0.4136047370352003 2119 7 -1.4457297898435684 2119 8 -0.06094344403977203 2119 9 -0.5776705741237578 2119 10-11 0.2477174463003493 2119 12-13 -0.13601461261285408 2119 14-15 0.024857690361670848 2119 16-17 -0.5709346237704267 2119 18-19 -0.3563476307351081 2119 20-21 -1.482272104716543 2119 22-23 -0.2970594914700939 2119 24-25 0.66605373505476 2119 26-27 0.4795964504272945 2119 28-29 -0.24188904809891199 2119 30-31 0.9351463278212648 2119 32-33 0.32245846747981943 2119 34-35 0.2747540370346826 2119 36-37 0.26947410812186234 2119 38-39 -1.3764068416567525 2119 40-41 -1.324700631514503 2119 42-43 -0.40164689035225365 2119 44-45 0.23987410595601233 2119 46-47 -0.31334782426767305 2119 48-49 -0.004131159017845221 2119 50-51 0.5091702733678787 2119 52-53 -0.19561694354984382 2119 54-55 0.05874406356989681 2119 56-57 -1.0964732359630318 2119 58-59 -0.30579531674330696 2119 60-61 0.10331132391802811 2119 62-63 -0.41697009764832416 2119 64-65 -0.014501173527037281 2119 66-67 0.5388929803582272 2119 68-69 2.035249660733797 2119 70-71 0.8191688272933817 2119 72-73 1.8012273042774822 2119 74-75 1.2718248589657577 2119 76 1.3474116929565767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 4.0 21 7.0 22 5.0 23 24.0 24 57.0 25 130.0 26 212.0 27 394.0 28 716.0 29 1188.0 30 1804.0 31 2665.0 32 3866.0 33 6020.0 34 8567.0 35 11015.0 36 14845.0 37 25561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.730257780776068 22.637225775482932 11.442508529987935 34.19000791375307 2 16.049350683047702 17.658048027399747 45.21736874197273 21.07523254757982 3 15.15159377797382 26.708267925948025 36.17363552626458 21.966502769813573 4 11.067578261828466 23.381896965529766 35.8233546528976 29.727170119744166 5 10.386476563614899 39.6284428069174 37.11939388435542 12.865686745112285 6 21.812119718218497 38.19877790895292 17.20138555545465 22.787716817373934 7 21.54876039490925 36.26574642259441 19.03063011637109 23.154863066125245 8 18.947600576017436 36.44737354211804 23.22491924079864 21.38010664106589 9 18.76467611992579 20.617272739066696 18.18087466431416 42.43717647669335 10-11 19.471075881215864 25.20335750703805 30.44784058328252 24.877726028463563 12-13 22.700795267316202 19.249879996367458 27.73056914155239 30.318755594763953 14-15 23.305354108016243 23.20935120198233 20.26050518285959 33.224789507141836 16-17 24.67663886041956 27.50353524214787 20.334453367237064 27.485372530195505 18-19 22.344676379393107 26.311931604416134 21.11804465432467 30.22534736186609 20-21 21.049285816219303 24.020835225282493 20.97274295870578 33.95713599979243 22-23 23.352706892749186 29.803064308973674 21.335997197753013 25.508231600524123 24-25 24.502795760304096 31.708203059119626 21.067448528171663 22.721552652404615 26-27 25.355794553781084 28.843035248634553 25.236439589522707 20.564730608061648 28-29 20.32926402096496 22.444571295131098 31.307326059599642 25.918838624304303 30-31 29.094718542831565 21.083665235271987 25.564017072949234 24.25759914894721 32-33 29.31007641312386 21.84779647383921 29.506622903179775 19.335504209857163 34-35 29.308130408271815 25.277305691415524 27.73056914155239 17.683994758760267 36-37 19.317990166188814 27.022872043694296 31.71923041994785 21.939907370169042 38-39 20.53618920356508 23.121780983640587 28.894280043071575 27.44774976972276 40-41 19.486643920032172 24.06040399060728 30.973910561617004 25.47904152774354 42-43 24.88096936988363 22.96934393689755 32.15189216538447 19.997794527834355 44-45 19.43020977932305 24.963998910237283 29.594841789805532 26.010949520634135 46-47 24.70128825521205 20.297479275048325 27.84473475953867 27.156497710200956 48-49 22.346622384245144 20.17423230108587 29.187478107445415 28.29166720722357 50-51 17.02105577249906 20.68927491859213 33.59258442417716 28.697084884731645 52-53 18.514290162296803 20.641922133859186 38.04374618907383 22.80004151477018 54-55 17.266901052139957 21.06485385503561 33.816374982161626 27.85187011066281 56-57 22.883719723407843 20.815116565690637 30.22405002529806 26.077113685603454 58-59 17.71383349982486 27.850572774094783 33.25916892619452 21.176424799885833 60-61 19.38091098973807 35.935574266031836 24.283545880307727 20.399968863922368 62-63 15.00304874093486 42.28149608853025 21.98142214034587 20.73403303018902 64-65 15.270300073948185 43.16627962792387 20.96949961728571 20.59392068084223 66-67 15.001102736082823 42.28149608853025 20.414239566170654 22.30316160921628 68-69 15.190513875014593 40.364681309272065 20.907876130304484 23.536928685408856 70-71 15.801559398554769 37.28480429677871 21.784875650289955 25.128760654376563 72-73 16.466444389668013 34.18611590404899 22.219483400578614 27.127956305704387 74-75 17.00548773368275 33.64512655518221 22.197428678922172 27.151957032212863 76 17.25198168160766 34.04989556440627 22.15721124531337 26.540911508672693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 2.0 16 2.0 17 2.0 18 3.5 19 7.5 20 18.0 21 26.0 22 26.0 23 41.0 24 67.5 25 79.0 26 129.0 27 204.0 28 280.5 29 332.0 30 385.0 31 508.5 32 729.0 33 879.0 34 979.0 35 1265.5 36 1600.0 37 1748.0 38 2013.5 39 2693.5 40 3556.5 41 5255.0 42 6505.0 43 7363.0 44 8367.5 45 7870.0 46 7226.0 47 6562.5 48 5408.5 49 4516.5 50 4115.0 51 3704.0 52 3030.5 53 2553.5 54 2339.0 55 2164.0 56 1698.0 57 1204.0 58 1001.0 59 828.0 60 601.0 61 470.5 62 394.0 63 319.0 64 215.0 65 149.5 66 108.0 67 103.0 68 88.0 69 45.5 70 17.5 71 17.0 72 14.0 73 8.0 74 3.5 75 2.0 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 77081.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.77294015386412 #Duplication Level Percentage of deduplicated Percentage of total 1 62.83424951085438 26.247713444298853 2 17.575079971427684 14.68325527691649 3 8.121370228889097 10.177605376162738 4 3.9908071679244697 6.668309959652833 5 2.1739805584024348 4.54067798809045 6 1.3261281406254852 3.3237762872822096 7 1.0372992950091617 3.033172896044421 8 0.6801453461287618 2.272933667181277 9 0.4627472902885183 1.7397283377226556 >10 1.726761700673934 10.346259130006096 >50 0.01863411907202087 0.5993694944279394 >100 0.04037392465604522 2.8541404496568545 >500 0.003105686512003478 0.9509477043629428 >1k 0.009317059536010435 12.562109988194237 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT 4178 5.420272181211972 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT 4030 5.228266369144148 No Hit CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 1475 1.9135714378381181 No Hit CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 733 0.9509477043629428 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 397 0.5150426175062597 No Hit TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT 333 0.4320130771526057 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 193 0.2503859576289877 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 172 0.22314188970044496 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 163 0.21146586058821243 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT 162 0.21016852402018657 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT 120 0.15568038816310115 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 118 0.15308571502704946 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT 116 0.15049104189099777 No Hit CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 114 0.1478963687549461 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT 109 0.14140968591481687 No Hit TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 102 0.132328329938636 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 101 0.13103099337061014 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT 87 0.11286828141824834 No Hit CTTATCACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 81 0.10508426201009329 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 80 0.10378692544206743 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGTCTT 78 0.10119225230601574 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009081355976180901 0.0 2 0.0 0.0 0.0 0.010378692544206744 0.0 3 0.0 0.0 0.0 0.010378692544206744 0.0 4 0.0 0.0 0.0 0.014270702248284273 0.0 5 0.0 0.0 0.0 0.014270702248284273 0.0 6 0.0 0.0 0.0 0.014270702248284273 0.0 7 0.0 0.0 0.0 0.014270702248284273 0.0 8 0.0 0.0 0.0 0.014270702248284273 0.0 9 0.0 0.0 0.0 0.014270702248284273 0.0 10 0.0 0.0 0.0 0.015568038816310116 0.0 11 0.0 0.0 0.0 0.015568038816310116 0.0 12 0.0 0.0 0.0 0.016865375384335957 0.0 13 0.0 0.0 0.0 0.019460048520387643 0.0 14 0.0 0.0 0.0 0.019460048520387643 0.0 15 0.0 0.0 0.0 0.02205472165643933 0.0 16 0.0 0.0 0.0 0.02205472165643933 0.0 17 0.0 0.0 0.0 0.028541404496568546 0.0 18 0.0 0.0 0.0 0.03762276047274945 0.0 19 0.0 0.0 0.0 0.0454067798809045 0.0 20 0.0 0.0 0.0 0.0454067798809045 0.0 21 0.0 0.0 0.0 0.0454067798809045 0.0 22 0.0 0.0 0.0 0.048001453016956186 0.0 23 0.0 0.0 0.0 0.048001453016956186 0.0 24 0.0 0.0 0.0 0.05319079928905956 0.0 25 0.0 0.0 0.0 0.05708280899313709 0.0 26 0.0 0.0 0.0 0.06746150153734383 0.0 27 0.0 0.0 0.0 0.09730024260193822 0.0 28 0.0 0.0 0.0 0.1297336568025843 0.0 29 0.0 0.0 0.0 0.17124842697941126 0.0 30 0.0 0.0 0.0 0.21016852402018657 0.0 31 0.0 0.0 0.0 0.23481791881267758 0.0 32 0.0 0.0 0.0 0.27892736212555624 0.0 33 0.0 0.0 0.0 0.33471283455066747 0.0 34 0.0 0.0 0.0 0.3930929801118304 0.0 35 0.0 0.0 0.0 0.5046639249620529 0.0 36 0.0 0.0 0.0 0.6914803907577743 0.0 37 0.0 0.0 0.0 0.9794891088595115 0.0 38 0.0 0.0 0.0 1.3349593284985923 0.0 39 0.0 0.0 0.0 1.8357312437565678 0.0 40 0.0 0.0 0.0 2.3170431104941556 0.0 41 0.0 0.0 0.0 3.0098208378199556 0.0 42 0.0 0.0 0.0 3.8115748368599265 0.0 43 0.0 0.0 0.0 4.750846512110637 0.0 44 0.0 0.0 0.0 5.4280562006201265 0.0 45 0.0 0.0 0.0 6.067643128656868 0.0 46 0.0 0.0 0.0 6.85642376201658 0.0 47 0.0 0.0 0.0 7.573850884134871 0.0 48 0.0 0.0 0.0 8.17322037856281 0.0 49 0.0 0.0 0.0 8.767400526718646 0.0 50 0.0 0.0 0.0 9.331741933809889 0.0 51 0.0 0.0 0.0 9.972626198414655 0.0 52 0.0 0.0 0.0 10.747136129526083 0.0 53 0.0 0.0 0.0 11.608567610695243 0.0 54 0.0 0.0 0.0 12.431078994823627 0.0 55 0.0 0.0 0.0 13.131640741557582 0.0 56 0.0 0.0 0.0 13.754362294209987 0.0 57 0.0 0.0 0.0 14.388759875974625 0.0 58 0.0 0.0 0.0 14.940127917385608 0.0 59 0.0 0.0 0.0 15.431818476667402 0.0 60 0.0 0.0 0.0 15.983186518078385 0.0 61 0.0 0.0 0.0 16.515094510968982 0.0 62 0.0 0.0 0.0 17.07035456208404 0.0 63 0.0 0.0 0.0 17.6606427005358 0.0 64 0.0 0.0 0.0 18.234065463603223 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGAG 15 0.0022170134 70.0 30 CCAGAAG 15 0.0022170134 70.0 60 CCGACCC 15 0.0022170134 70.0 9 GCCGTTG 15 0.0022170134 70.0 28 TTTACAC 205 0.0 63.17073 2 CTTTACA 225 0.0 57.555557 1 TTAATAC 25 2.3770155E-4 55.999996 2 ATATCCG 25 2.3770155E-4 55.999996 10 TATCCGA 25 2.3770155E-4 55.999996 11 CTATACA 120 0.0 55.416664 1 TCAAAAT 20 0.0069275685 52.500004 46 AGCCGTT 20 0.0069275685 52.500004 27 AGTCATC 20 0.0069275685 52.500004 66 GTACACT 20 0.0069275685 52.500004 41 ACCATAT 20 0.0069275685 52.500004 40 CGGCAGG 20 0.0069275685 52.500004 70 GCCGGGA 20 0.0069275685 52.500004 44 TTCGTCC 20 0.0069275685 52.500004 20 TTTCCAG 20 0.0069275685 52.500004 35 TAGCCGT 20 0.0069275685 52.500004 26 >>END_MODULE