FastQCFastQC Report
Wed 25 May 2016
SRR1780407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650401
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATT300.005768219529.30713332
TGACACG300.005776897529.2981199
CGTGTAT609.695203E-625.68324735
GGTCATA609.776637E-625.655581
GGTCTAA450.001401040924.4338841
CGGTGGT2500.023.7369543
CTATACA2350.023.3941481
GGTAGAC500.002570659222.01251611
TCGGTGG3800.021.97865942
TAACTGA500.002597217721.9735896
TATATCG602.895721E-421.97195
TACACAG904.1262865E-721.9718995
GTCTACT1101.2691453E-719.9913621
CGAAACT1252.2842869E-819.35908522
CATCGTT809.042259E-519.23280532
GCATGTA809.068998E-519.2254122
TCTACTG1152.0451444E-719.1059992
GACGACC1504.5656634E-1019.0922220
GATACCT1051.7975035E-618.84899728
AATTATC1303.594323E-818.60877630
Produced by FastQC (version 0.11.5)