FastQCFastQC Report
Wed 25 May 2016
SRR1780407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650401
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT442506.803495074577069No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT350945.395748161518816Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC116551.7919714145580956No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC62690.9638669067236982No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG48790.7501525981663619No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT33700.5181418847756999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC24720.3800732163695935No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC15770.2424658018668483No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG14090.2166355832786235No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC10510.1615926174775254No Hit
CTTATAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG10080.15498131152934883No Hit
CTTAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC9430.14498747695652373No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC8080.12423105130527168No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG8020.12330854349854936No Hit
CTTATCACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC7060.10854841859099233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT37500.042.23719444
GCCGTCT41750.037.8849143
CAGTTAC357.2426265E-637.746611
CTATACA8800.037.0317081
TTTACAC12900.036.672692
CTTTACA13400.035.8217161
CGTAGCG505.9222657E-835.21119715
CTTATAA1600.033.0282821
TTACACA14600.032.4025843
GCGGAAT551.3737917E-731.99541519
TTATACA67100.029.5130422
AGTGGTC300.005744950429.33138742
CGGAATC602.945526E-729.3291320
CTTATAC68000.029.2397441
TCTTTAT2650.027.4197041
CAGACTA508.287174E-526.4226251
TATACAC77100.025.999083
ATTCAAC609.748957E-625.66496344
AATACAT609.754776E-625.66298929
TGCCGTC62850.025.16618742
Produced by FastQC (version 0.11.5)