FastQCFastQC Report
Wed 25 May 2016
SRR1780406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99473
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTGC150.002219675669.9939652
GAGACAT150.002219675669.99396519
AAGGCAC150.002219675669.99396518
AGGCGTC200.00693583552.49547618
CAAGGCA200.00693583552.49547617
CGCAGAA200.00693583552.49547658
GACATAC200.00693583552.49547621
CCCGGCC200.00693583552.49547622
TGGTCTT200.00693583552.49547655
CAAACCG305.8558804E-446.6626432
CCGACAA305.8558804E-446.66264321
GTCTACC350.001250776539.9965539
AGTCTAC350.001250776539.9965538
CCCTTAG350.001250776539.9965548
TCCCGAC350.001250776539.9965519
AACAGTC350.001250776539.9965535
CTTGCCC400.002409876634.99698325
GAACAGT400.002409876634.99698334
AACAGGT400.002409876634.99698326
TCCTTGC450.004291553531.1084323
Produced by FastQC (version 0.11.5)