##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780406_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99473 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.995134358067013 34.0 24.0 34.0 12.0 34.0 2 29.89115639419742 34.0 31.0 34.0 12.0 34.0 3 30.188422989152837 34.0 31.0 34.0 11.0 34.0 4 30.497310828063895 34.0 32.0 34.0 21.0 34.0 5 30.490525067103636 34.0 32.0 34.0 21.0 34.0 6 32.94394458797865 37.0 34.0 38.0 11.0 38.0 7 32.31422597086647 37.0 31.0 38.0 11.0 38.0 8 31.4478903823148 37.0 27.0 38.0 11.0 38.0 9 32.11918812139978 37.0 31.0 38.0 12.0 38.0 10-11 32.450202567530894 37.0 31.5 38.0 11.0 38.0 12-13 32.806796819237384 37.0 33.0 38.0 11.0 38.0 14-15 32.39455932765675 37.0 31.0 38.0 11.0 38.0 16-17 32.1875634594312 37.0 31.0 38.0 11.0 38.0 18-19 32.23950217647 37.0 31.0 38.0 11.0 38.0 20-21 32.33915233279382 37.0 31.0 38.0 11.0 38.0 22-23 32.22250258864214 37.0 31.0 38.0 11.0 38.0 24-25 32.60691846028571 37.0 32.0 38.0 11.0 38.0 26-27 32.58917495199702 37.0 32.5 38.0 11.0 38.0 28-29 32.66107888572778 37.0 33.5 38.0 11.0 38.0 30-31 32.5416997577232 37.0 32.0 38.0 11.0 38.0 32-33 32.37913303107376 37.0 31.5 38.0 11.0 38.0 34-35 32.32517366521569 37.0 31.0 38.0 11.0 38.0 36-37 32.25476762538578 37.0 31.0 38.0 11.0 38.0 38-39 32.622701637630314 37.0 33.0 38.0 11.0 38.0 40-41 32.256923989424266 37.0 31.0 38.0 11.0 38.0 42-43 32.5651332522393 37.0 32.5 38.0 11.0 38.0 44-45 31.746906195651082 37.0 29.0 38.0 11.0 38.0 46-47 32.367310727534104 37.0 31.0 38.0 11.0 38.0 48-49 32.179968433645314 37.0 31.0 38.0 11.0 38.0 50-51 32.17337367928986 37.0 31.0 38.0 11.0 38.0 52-53 32.14007821217818 37.0 31.0 38.0 11.0 38.0 54-55 31.92388386798428 37.0 31.0 38.0 11.0 38.0 56-57 31.911116584399785 37.0 29.0 38.0 11.0 38.0 58-59 31.775119881776963 37.0 29.0 38.0 11.0 38.0 60-61 31.869105184321374 37.0 29.5 38.0 11.0 38.0 62-63 31.895730499733595 37.0 31.0 38.0 11.0 38.0 64-65 31.952931951383793 37.0 31.0 38.0 11.0 38.0 66-67 31.935495058960722 37.0 30.5 38.0 11.0 38.0 68-69 31.625722557880028 37.0 28.5 38.0 11.0 38.0 70-71 31.556683723221376 37.0 27.0 38.0 11.0 38.0 72-73 31.63229217978748 37.0 28.5 38.0 11.0 38.0 74-75 31.20258763684618 36.5 27.0 38.0 11.0 38.0 76 30.429322529731685 36.0 25.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.873932969728898 1101 2 -1.1155645586627152 1101 3 0.10841023198323896 1101 4 -1.7672949061493064 1101 5 -0.4029348710464866 1101 6 -1.95085997371854 1101 7 -1.3240874163605518 1101 8 -0.8848625848627911 1101 9 0.12706976543119453 1101 10-11 0.09935982976208635 1101 12-13 -0.5704986225707103 1101 14-15 -0.4105361381363366 1101 16-17 -2.145832079345361 1101 18-19 -0.1189283397372165 1101 20-21 -0.10647293825719117 1101 22-23 -0.37127207490760483 1101 24-25 -1.3840215819660955 1101 26-27 -0.825619753189784 1101 28-29 -0.01173526668578262 1101 30-31 -0.6477873774712535 1101 32-33 -0.12046986657805547 1101 34-35 -0.8439918688989678 1101 36-37 0.05381060431580664 1101 38-39 -0.6364258032294856 1101 40-41 0.03805860049565979 1101 42-43 -0.6508217615318515 1101 44-45 -0.33779129691097154 1101 46-47 0.5489308872065024 1101 48-49 -0.8688589094825403 1101 50-51 -0.8961255293310941 1101 52-53 -0.07710582480698491 1101 54-55 -1.012890708956668 1101 56-57 -0.6355247989047363 1101 58-59 -0.17169346407656505 1101 60-61 -0.2972820053689631 1101 62-63 -1.230546901476199 1101 64-65 0.07331820173541814 1101 66-67 -0.6913285919160401 1101 68-69 -0.5565738595199186 1101 70-71 0.05214793252200067 1101 72-73 -0.3547331362318147 1101 74-75 -1.14482986948968 1101 76 -2.0552762797482593 1102 1 -0.007459220245639386 1102 2 -0.4809359242407787 1102 3 -0.39083187908194006 1102 4 0.6942594551570167 1102 5 0.20968053476858728 1102 6 0.6944480047849737 1102 7 1.4168523659186292 1102 8 1.346844732210382 1102 9 0.3116846200543222 1102 10-11 -0.01821630362636739 1102 12-13 -3.4973278908633922 1102 14-15 -1.5511934345398117 1102 16-17 -1.7003484084069527 1102 18-19 -0.5807238475228047 1102 20-21 0.7208834073401604 1102 22-23 -0.859473122172318 1102 24-25 0.3482024775626371 1102 26-27 -4.120851942799817 1102 28-29 0.06760192499121587 1102 30-31 0.24784787125411611 1102 32-33 -6.5167596557471335 1102 34-35 -0.3564970363235247 1102 36-37 0.2553229372357393 1102 38-39 0.3679665076427767 1102 40-41 0.3967467636484798 1102 42-43 -0.06509418815442558 1102 44-45 -4.819188568510803 1102 46-47 -0.25017687082023343 1102 48-49 0.6925989932320746 1102 50-51 -5.365499926464892 1102 52-53 -1.5098948560871328 1102 54-55 0.3787139486153315 1102 56-57 -1.1462558172318715 1102 58-59 0.5438053646405372 1102 60-61 -0.12458667672076018 1102 62-63 -4.18722665895174 1102 64-65 -0.3370269822248986 1102 66-67 -0.3579608090856681 1102 68-69 -7.093538169840986 1102 70-71 -0.644334773830483 1102 72-73 -0.1697393371412801 1102 74-75 -5.609605258768994 1102 76 -2.1727490646456005 1103 1 -0.877118448752821 1103 2 -0.5349058256419212 1103 3 -0.0034421541208615736 1103 4 -0.4031285399995319 1103 5 -0.25174482416585064 1103 6 -1.70323405714203 1103 7 0.1363645610405797 1103 8 0.9165558548535238 1103 9 -0.6455892080031091 1103 10-11 -0.6628712076222136 1103 12-13 -2.230073090655818 1103 14-15 -1.224606338881621 1103 16-17 -1.011756469296074 1103 18-19 -1.3496652071491653 1103 20-21 -0.9879039996828425 1103 22-23 -1.666124220598192 1103 24-25 -0.48833099415506354 1103 26-27 -1.6698919012845082 1103 28-29 -0.5181963798003437 1103 30-31 -0.02615316662860323 1103 32-33 -4.7738086092153935 1103 34-35 -1.2395622499543855 1103 36-37 -0.4918228852863109 1103 38-39 -0.12234658697753531 1103 40-41 0.09831223675001866 1103 42-43 -0.4103631727591619 1103 44-45 -5.192220915855554 1103 46-47 -0.8853610950029029 1103 48-49 -0.21727819064614096 1103 50-51 -0.34344491868077043 1103 52-53 -0.24605641983043114 1103 54-55 -0.22338776389115722 1103 56-57 0.2604212282619862 1103 58-59 -0.06616695848694931 1103 60-61 -0.21103365821184994 1103 62-63 -0.22337957367237493 1103 64-65 -0.2513151676599463 1103 66-67 -0.9649492193953009 1103 68-69 -3.4900093685955262 1103 70-71 -0.34019189197613287 1103 72-73 -0.04856652966852337 1103 74-75 -2.514120048597743 1103 76 -0.12950395134340553 1104 1 -1.8792563497692818 1104 2 -0.23583011578197244 1104 3 -0.20435364245841825 1104 4 -0.08345472992707315 1104 5 -0.7791662851075536 1104 6 -0.5539010310519572 1104 7 -0.19335866398950685 1104 8 0.337159075787028 1104 9 -0.6115492151721185 1104 10-11 -0.8762260479837352 1104 12-13 -0.5768163121013998 1104 14-15 -0.2499475069180761 1104 16-17 -1.2206882868959923 1104 18-19 0.03624512902616317 1104 20-21 0.384218795206678 1104 22-23 -0.22090953437037086 1104 24-25 -3.7108940047403394E-4 1104 26-27 0.07592831020126312 1104 28-29 -0.6762858387961685 1104 30-31 -1.180683724531157 1104 32-33 -1.0365271999929746 1104 34-35 -0.5019799983733613 1104 36-37 -0.3592026123518828 1104 38-39 -0.9345121398873815 1104 40-41 0.3363361505170701 1104 42-43 0.2698093955384522 1104 44-45 -3.296229160216363 1104 46-47 -0.3704918014063665 1104 48-49 0.5090704792465814 1104 50-51 0.6953509437766598 1104 52-53 -0.40169433364869533 1104 54-55 -0.8740009702306075 1104 56-57 -0.011962723398404762 1104 58-59 0.4384224877070757 1104 60-61 0.008965317643948367 1104 62-63 0.10679171444499147 1104 64-65 -0.14445852224427114 1104 66-67 -0.26954250613007247 1104 68-69 -3.1700475179669603 1104 70-71 -0.6896298928466571 1104 72-73 0.06885800370577755 1104 74-75 -1.2422033627283717 1104 76 -0.32958332251886446 1105 1 -0.6655752841649019 1105 2 0.16526536535832648 1105 3 -0.38673880366842894 1105 4 0.15245962336559415 1105 5 0.6398179051078081 1105 6 -0.4486974956355496 1105 7 0.6926864001210404 1105 8 0.7875512082406537 1105 9 0.5444572581255258 1105 10-11 0.47250418978546094 1105 12-13 -1.375671036363812 1105 14-15 0.10170816344168543 1105 16-17 0.8320317429807673 1105 18-19 0.8243504445994247 1105 20-21 0.06373454274806534 1105 22-23 0.3844994037598397 1105 24-25 -0.40924075204206645 1105 26-27 0.046151141024125764 1105 28-29 -0.5031437997074093 1105 30-31 -0.1358499139995999 1105 32-33 0.34459442331090173 1105 34-35 0.04493273659489461 1105 36-37 0.5748070975945936 1105 38-39 0.2267974580212453 1105 40-41 0.3997184485377403 1105 42-43 0.44255930217358497 1105 44-45 1.3422496148365468 1105 46-47 0.3357076259557701 1105 48-49 0.011971014538495695 1105 50-51 0.043471197729111566 1105 52-53 -0.4306125460226511 1105 54-55 -0.3673471671682371 1105 56-57 -0.11847336699357314 1105 58-59 -0.284215381084163 1105 60-61 -0.14035623096802397 1105 62-63 0.6558207197979122 1105 64-65 0.6460680556220311 1105 66-67 0.45262231398694297 1105 68-69 1.00134548589212 1105 70-71 0.8083285286646138 1105 72-73 0.3116315627746147 1105 74-75 -1.1179596181746554 1105 76 -0.8037274778780095 1106 1 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-0.20491840166689457 1106 60-61 0.31420137395044634 1106 62-63 0.10451090958673959 1106 64-65 0.17805148251324 1106 66-67 0.32075917578859503 1106 68-69 0.7561971298375916 1106 70-71 0.06014622446787499 1106 72-73 0.062393391162750333 1106 74-75 -1.4662889418848941 1106 76 -0.8249517494631462 1107 1 0.3503490453370155 1107 2 0.5129665147836135 1107 3 0.6541376331131801 1107 4 -0.20055761801371474 1107 5 -0.008984776884528145 1107 6 0.23899407524567806 1107 7 0.1363645610405797 1107 8 1.1359707484705446 1107 9 -0.6244603663955459 1107 10-11 0.09170917062838413 1107 12-13 0.07880693298484687 1107 14-15 -0.4356361852173194 1107 16-17 0.3481356701838294 1107 18-19 0.8038882798484721 1107 20-21 0.3475348301043937 1107 22-23 -0.0601845042861342 1107 24-25 -0.430337495337092 1107 26-27 0.17382116018121252 1107 28-29 -0.2902486847648831 1107 30-31 -0.20925748104940567 1107 32-33 0.7439957643070656 1107 34-35 0.45358934579029153 1107 36-37 0.05372232747964745 1107 38-39 0.5289167108948121 1107 40-41 0.6423429696105387 1107 42-43 0.46988209910845313 1107 44-45 0.442752488399762 1107 46-47 0.10060226197109046 1107 48-49 0.337797163963792 1107 50-51 0.7864309678440549 1107 52-53 0.1974645612570427 1107 54-55 0.7455838673145188 1107 56-57 1.1643810391366891 1107 58-59 0.817772166808556 1107 60-61 0.17567585715458023 1107 62-63 -0.18049393537450342 1107 64-65 0.25104889853390944 1107 66-67 0.6199355323777525 1107 68-69 1.1445207732484413 1107 70-71 -0.16465997708251834 1107 72-73 0.23778098806197434 1107 74-75 -0.10295722353864178 1107 76 -0.5180234548895051 1108 1 -0.1793193018357826 1108 2 0.5162286737397217 1108 3 0.3960934456873204 1108 4 0.16665768447738927 1108 5 0.49622973478016164 1108 6 0.7212251942650525 1108 7 1.226518772191227 1108 8 1.864247735421909 1108 9 0.17820373008527923 1108 10-11 0.3470225355947676 1108 12-13 0.7026330500271598 1108 14-15 0.8921057582385927 1108 16-17 -0.4380013899189734 1108 18-19 0.27268152816795777 1108 20-21 0.6039664730462633 1108 22-23 -0.16731677000380785 1108 24-25 -0.13376523357354841 1108 26-27 0.1429851083820779 1108 28-29 0.6691411432303767 1108 30-31 1.0255870721336677 1108 32-33 0.7516198252006987 1108 34-35 0.4795198024925469 1108 36-37 0.8076332112281577 1108 38-39 1.2207353645128407 1108 40-41 0.5765560973290036 1108 42-43 0.810567646755743 1108 44-45 1.2132521918398567 1108 46-47 1.044972566439263 1108 48-49 0.3981656396458746 1108 50-51 0.8464102086506955 1108 52-53 0.6894512654879605 1108 54-55 1.0529682089515298 1108 56-57 0.8426629687531424 1108 58-59 -0.23821587194193583 1108 60-61 0.9586743249284027 1108 62-63 0.5050598902482726 1108 64-65 0.722776854247634 1108 66-67 0.6415473354619721 1108 68-69 1.599406597684979 1108 70-71 1.8083530715533094 1108 72-73 0.6560787342066945 1108 74-75 1.4939375471216998 1108 76 1.2595195462967368 1109 1 0.46308997504223015 1109 2 -0.760843009025912 1109 3 0.3290951161063802 1109 4 0.06517367450329203 1109 5 0.6252272412560629 1109 6 1.2414033722978317 1109 7 -0.6731592484832305 1109 8 0.972280272280063 1109 9 0.9026087946021875 1109 10-11 1.2185515742565016 1109 12-13 0.28664423457215094 1109 14-15 -0.008800867757003061 1109 16-17 -0.5651650667776238 1109 18-19 0.7300858422067407 1109 20-21 0.9260850001724208 1109 22-23 1.08734950931931 1109 24-25 1.1831120511481714 1109 26-27 1.0205119198184036 1109 28-29 0.9743133900650491 1109 30-31 0.8140914418530869 1109 32-33 1.4141346531959584 1109 34-35 0.8820544818447189 1109 36-37 1.0650956267993692 1109 38-39 1.3389719830036526 1109 40-41 1.0604268698372934 1109 42-43 0.8669200809678586 1109 44-45 0.8409242571072539 1109 46-47 0.2316963662794791 1109 48-49 0.509672163963792 1109 50-51 1.4399173624018715 1109 52-53 1.6296853662457025 1109 54-55 0.7056178809199629 1109 56-57 1.2695397692954238 1109 58-59 1.191142348214452 1109 60-61 1.0704108344788423 1109 62-63 0.5137024931969272 1109 64-65 0.9931123905974033 1109 66-67 1.1928521990444167 1109 68-69 1.6902265555613702 1109 70-71 0.46629240386986837 1109 72-73 0.10767894724564542 1109 74-75 1.7523077991370961 1109 76 0.13652586483838647 1110 1 -0.1411604842655798 1110 2 -0.841656356262618 1110 3 -1.0904639483953353 1110 4 -1.2429846253129853 1110 5 -0.6698221653679006 1110 6 -0.6624150893934129 1110 7 -0.17872059711027077 1110 8 0.3961593129474288 1110 9 -1.7335945918618876 1110 10-11 -1.4740349494040643 1110 12-13 -0.6234183305999039 1110 14-15 -0.993333366888038 1110 16-17 -1.5051819494430951 1110 18-19 -0.5922365173940278 1110 20-21 -1.356426544591475 1110 22-23 -0.44963097630560256 1110 24-25 -0.426054243106762 1110 26-27 -1.5299374570126218 1110 28-29 -1.023179037562933 1110 30-31 -0.908225952257844 1110 32-33 -0.16189941006438602 1110 34-35 -0.4804511043313582 1110 36-37 -1.3470152029339815 1110 38-39 -1.400637729494484 1110 40-41 -1.4433220749961109 1110 42-43 -0.741923456447104 1110 44-45 -0.051697024982232165 1110 46-47 -0.9733535368934696 1110 48-49 -0.591301072045944 1110 50-51 -1.008429129479552 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2116 38-39 0.11084073050265175 2116 40-41 -0.0038988604548180206 2116 42-43 0.6512137984548545 2116 44-45 0.9678750374193683 2116 46-47 0.4006839613174975 2116 48-49 0.15869177180692162 2116 50-51 -0.02410824784222143 2116 52-53 0.7429506723681527 2116 54-55 0.03234857319687379 2116 56-57 0.49951175809094295 2116 58-59 -0.469441885479025 2116 60-61 -0.9146631951330022 2116 62-63 0.08120949599804561 2116 64-65 0.6111796174881547 2116 66-67 0.22716592453461004 2116 68-69 0.4193982242288321 2116 70-71 -2.328385467278597 2116 72-73 0.1363103998266766 2116 74-75 -0.5234024849765468 2116 76 -1.0411852195953877 2117 1 0.36390191272052874 2117 2 0.1285579126330738 2117 3 0.14640913848952408 2117 4 0.01920044650241337 2117 5 -0.7027571783182189 2117 6 -0.9278966057579083 2117 7 -1.975194578744368 2117 8 -2.134862584862791 2117 9 -0.1676951334206329 2117 10-11 0.020696625825515014 2117 12-13 0.2157917000099303 2117 14-15 -0.18401208127466617 2117 16-17 0.19451783864261074 2117 18-19 0.051144104221229725 2117 20-21 -0.6160068365622777 2117 22-23 -0.45803396665172613 2117 24-25 0.17102899211811007 2117 26-27 0.7624748340163379 2117 28-29 0.3137499711490932 2117 30-31 0.3884687734308798 2117 32-33 0.8812045456690711 2117 34-35 0.1963648834247067 2117 36-37 -1.2821311671440085 2117 38-39 0.40912504422814067 2117 40-41 -0.4333738762675985 2117 42-43 -0.4415202306406556 2117 44-45 0.6440293701201938 2117 46-47 -0.129684476380163 2117 48-49 -0.25376869625126375 2117 50-51 0.5673449463386788 2117 52-53 0.8420098121531012 2117 54-55 -0.7846580681693531 2117 56-57 -0.9523304303973568 2117 58-59 -0.5309061486036306 2117 60-61 0.8409424342155063 2117 62-63 -0.29793345150353545 2117 64-65 -0.6465765494947604 2117 66-67 -1.5376585536437588 2117 68-69 -0.482831377289191 2117 70-71 0.8259314207669455 2117 72-73 0.716275611717883 2117 74-75 0.1329858768197809 2117 76 -0.17091323983574114 2118 1 0.30409499018697517 2118 2 1.0853509430074553 2118 3 0.19785721948787582 2118 4 0.7168693892855558 2118 5 0.22068878353720933 2118 6 1.5200666624314039 2118 7 0.9234691839116209 2118 8 -2.9505560155197266 2118 9 -0.6496529860224705 2118 10-11 -0.08717363553544999 2118 12-13 0.8820561219548324 2118 14-15 -0.3218005201727152 2118 16-17 0.38532899946201127 2118 18-19 -0.19807593345242225 2118 20-21 -2.77066012123381 2118 22-23 -0.13393754564867066 2118 24-25 0.3385442970392276 2118 26-27 0.7474348057610953 2118 28-29 -0.1618651689497952 2118 30-31 -0.5724595289001755 2118 32-33 0.3709473052640817 2118 34-35 1.1910817423839717 2118 36-37 0.5270723883068982 2118 38-39 0.45520168656390325 2118 40-41 -0.8210593012734861 2118 42-43 0.13216413479377564 2118 44-45 0.47932487282798775 2118 46-47 0.2602231054423001 2118 48-49 -0.6661185902941185 2118 50-51 0.07003802380511814 2118 52-53 -0.9448838775101933 2118 54-55 0.1260583198692622 2118 56-57 1.053776821780648 2118 58-59 0.7485558245538932 2118 60-61 -1.043137504564804 2118 62-63 0.9500945682751301 2118 64-65 -0.06788865215546735 2118 66-67 -1.9817220102013273 2118 68-69 1.134104106581777 2118 70-71 -0.43534124228932924 2118 72-73 -0.9060754596265923 2118 74-75 0.6359291308814718 2118 76 0.03299540155818548 2119 1 0.6209131042939298 2119 2 0.3779325011781687 2119 3 0.41276858549413475 2119 4 0.6461260554556745 2119 5 0.6286286018002798 2119 6 0.5951448688964689 2119 7 0.12139857464602244 2119 8 1.6355455784025175 2119 9 0.6236972299763366 2119 10-11 0.8875991933041192 2119 12-13 0.6611340304905156 2119 14-15 -0.28601175211073837 2119 16-17 0.4161274502291832 2119 18-19 0.27702461771694686 2119 20-21 -0.45248642839901265 2119 22-23 1.0220433868703225 2119 24-25 0.50726171101212 2119 26-27 0.4524847089340511 2119 28-29 0.9379188322419196 2119 30-31 0.6899417819891411 2119 32-33 0.852229891291195 2119 34-35 0.612666726742674 2119 36-37 -0.39612886299654804 2119 38-39 0.2634617789220144 2119 40-41 -0.006579932883795436 2119 42-43 0.08766838028758883 2119 44-45 -0.41791928030771786 2119 46-47 0.11945146832029963 2119 48-49 -0.3406679720906354 2119 50-51 0.6501214440345251 2119 52-53 -0.650246606543412 2119 54-55 -0.3630896020732344 2119 56-57 0.9443697012682115 2119 58-59 1.3795777223641146 2119 60-61 1.0949006303972055 2119 62-63 0.5341106564622287 2119 64-65 1.0308674926382153 2119 66-67 1.005437233058018 2119 68-69 0.047369412704224345 2119 70-71 0.29826519298551446 2119 72-73 -0.9477632296251031 2119 74-75 0.4910833093411746 2119 76 0.9589748444302231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 25.0 13 144.0 14 599.0 15 1389.0 16 1952.0 17 2331.0 18 2611.0 19 2439.0 20 2086.0 21 1735.0 22 1381.0 23 1112.0 24 1026.0 25 1096.0 26 1223.0 27 1277.0 28 1575.0 29 1981.0 30 2415.0 31 2985.0 32 3773.0 33 5055.0 34 6900.0 35 10684.0 36 18067.0 37 23607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.6601322411122 27.203596634230802 10.342348545449022 27.79392257920797 2 17.945573170609112 22.741849547113286 31.319051400882653 27.993525881394955 3 16.061644868456767 29.08930061423703 27.9010384727514 26.9480160445548 4 12.020347229901581 24.84191690207393 34.21632000643391 28.92141586159058 5 11.43827973419923 39.1694228584641 32.818955897580246 16.573341509756418 6 23.234445527932202 37.60015280528385 17.34842620610618 21.81697546067777 7 24.596624209584512 32.50630824444824 20.73125370703608 22.165813838931168 8 22.591054859107494 34.90897027334051 19.444472369386666 23.055502498165332 9 22.25729594965468 19.729976978677634 20.513104058387704 37.49962301327999 10-11 22.039648949966324 26.878650488072143 26.34735053733174 24.7343500246298 12-13 21.952188030922965 24.789138761271904 25.659224111065313 27.59944909673982 14-15 20.891598725282236 26.08647572708172 23.10777799000734 29.914147557628702 16-17 21.74710725523509 28.20111990188292 24.629296391985765 25.422476450896227 18-19 20.605591467031253 27.394368321051942 25.18271289696702 26.817327314949786 20-21 19.963708745086606 27.79196364842721 25.28072944417078 26.9635981623154 22-23 22.229147607893598 27.189790194324086 24.987685100479524 25.593377097302785 24-25 20.901149055522605 28.770621173584793 24.66749771294723 25.660732057945374 26-27 19.77421008715933 29.322529731685986 25.973379711077378 24.929880470077308 28-29 21.074562946729262 27.725613985704662 27.36420938345079 23.835613684115287 30-31 21.239431805615595 27.57130075497874 27.05005378343872 24.139213655966945 32-33 19.719923999477246 29.494435676012586 26.30965186533029 24.475988459179877 34-35 20.741809335196486 28.20664904044314 26.42877966885486 24.622761955505513 36-37 20.9167311732832 27.653232535461886 26.58158495270073 24.84845133855418 38-39 20.397494797583263 27.20336171624461 27.79598484010737 24.60315864606476 40-41 20.199953756295677 26.451901521015753 27.79900073386748 25.549143988821086 42-43 19.173544579936262 26.30563067365014 28.177998049722035 26.342826696691564 44-45 18.797563157841825 26.729363746946404 28.683160254541434 25.789912840670333 46-47 19.44246177354659 27.043519346958472 28.15387089964111 25.36014797985383 48-49 18.643249927115903 27.615533863460435 27.877413971630492 25.86380223779317 50-51 18.403486373186695 27.32248951976918 27.30791269992862 26.966111407115502 52-53 18.114965870135617 27.22899681320559 27.575824595618915 27.08021272103988 54-55 17.807847355563823 27.61000472490022 27.26217164456687 27.319976274969086 56-57 18.65782674695646 27.213917344405015 27.183758406803854 26.944497501834668 58-59 18.48290490886974 27.091773647120327 26.810290229509516 27.61503121450042 60-61 18.19036321413851 26.578569058940616 26.97063524775567 28.2604324791652 62-63 18.5728790727132 26.848491550470982 26.64089752998301 27.937731846832808 64-65 18.760367134800397 27.352648457370343 25.537583062740644 28.349401345088616 66-67 18.632694298955496 28.18151659244217 24.989695696319604 28.19609341228273 68-69 18.265760558141405 28.435856966211936 24.87710232927528 28.421280146371373 70-71 18.488936696389977 29.107898625757745 24.584560634544047 27.818604043308238 72-73 18.582932051913584 29.266735697123842 24.185457360288723 27.96487489067385 74-75 19.18359755913665 29.588933680496215 24.135192464286792 27.092276296080342 76 19.511827330029256 29.10337478511757 24.188473254048837 27.19632463080434 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.5 10 2.0 11 1.5 12 1.0 13 3.0 14 5.5 15 9.0 16 11.5 17 11.0 18 18.0 19 25.0 20 32.5 21 40.0 22 54.5 23 81.5 24 118.5 25 143.0 26 199.0 27 312.5 28 474.0 29 578.0 30 679.5 31 865.0 32 1221.5 33 1494.0 34 1651.5 35 2095.5 36 2578.0 37 2774.0 38 3259.5 39 4194.5 40 5100.5 41 5899.0 42 6241.0 43 6356.5 44 6856.0 45 7245.5 46 7251.0 47 7311.0 48 7054.5 49 6474.5 50 6211.0 51 5832.5 52 4915.0 53 4010.5 54 3645.0 55 3398.0 56 2766.5 57 2087.0 58 1792.0 59 1581.0 60 1223.0 61 894.0 62 712.0 63 637.5 64 504.5 65 388.5 66 284.0 67 237.0 68 207.0 69 158.0 70 116.5 71 94.0 72 75.0 73 45.5 74 27.5 75 20.0 76 21.0 77 17.5 78 12.0 79 11.0 80 10.0 81 7.0 82 5.0 83 5.0 84 7.5 85 6.0 86 3.5 87 5.0 88 5.0 89 3.5 90 3.0 91 4.0 92 4.0 93 4.5 94 3.0 95 1.5 96 2.0 97 2.0 98 3.0 99 6.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7177827149075628 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 99473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.42324047731546 #Duplication Level Percentage of deduplicated Percentage of total 1 79.69852113680673 50.54738471746102 2 9.410515303777203 11.936907502538377 3 4.133842666708935 7.865450926382033 4 2.0304648987937672 5.151146542277804 5 1.3266972055350377 4.207171795361556 6 0.8892199908066383 3.3838327988499395 7 0.6165892627874907 2.7374262362651174 8 0.40260584253990395 2.042765373518442 9 0.33127803579070836 1.8909653875926131 >10 1.1602656564535816 10.236948719753098 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004021191680154414 0.0 2 0.0 0.0 0.0 0.004021191680154414 0.0 3 0.0 0.0 0.0 0.004021191680154414 0.0 4 0.0 0.0 0.0 0.005026489600193017 0.0 5 0.0 0.0 0.0 0.007037085440270224 0.0 6 0.0 0.0 0.0 0.007037085440270224 0.0 7 0.0 0.0 0.0 0.007037085440270224 0.0 8 0.0 0.0 0.0 0.008042383360308828 0.0 9 0.0 0.0 0.0 0.012063575040463242 0.0 10 0.0 0.0 0.0 0.013068872960501845 0.0 11 0.0 0.0 0.0 0.015079468800579051 0.0 12 0.0 0.0 0.0 0.016084766720617656 0.0 13 0.0 0.0 0.0 0.01709006464065626 0.0 14 0.0 0.0 0.0 0.018095362560694862 0.0 15 0.0 0.0 0.0 0.019100660480733465 0.0 16 0.0 0.0 0.0 0.022116554240849275 0.0 17 0.0 0.0 0.0 0.024127150080926484 0.0 18 0.0 0.0 0.0 0.027143043841042293 0.0 19 0.0 0.0 0.0 0.03216953344123531 0.0 20 0.0 0.0 0.0 0.036190725121389725 0.0 21 0.0 0.0 0.0 0.043227810561659946 0.0 22 0.0 0.0 0.0 0.04423310848169855 0.0 23 0.0 0.0 0.0 0.046243704321775755 0.0 24 0.0 0.0 0.0 0.04925959808189157 0.0 25 0.0 0.0 0.0 0.050264896001930175 0.0 26 0.0 0.0 0.0 0.057301981442200396 0.0 27 0.0 0.0 0.0 0.06735496064258643 0.0 28 0.0 0.0 0.0 0.08545032320328129 0.0 29 0.0 0.0 0.0 0.1156092608044394 0.0 30 0.0 0.0 0.0 0.13973641088536587 0.0 31 0.0 0.0 0.0 0.19402249856745046 0.0 32 0.0 0.0 0.0 0.22116554240849276 0.0 33 0.0 0.0 0.0 0.29857348225146524 0.0 34 0.0 0.0 0.0 0.3810079116946307 0.0 35 0.0 0.0 0.0 0.471484724498105 0.0 36 0.0 0.0 0.0 0.6092105395433937 0.0 37 0.0 0.0 0.0 0.8374131673921567 0.0 38 0.0 0.0 0.0 1.1872568435655906 0.0 39 0.0 0.0 0.0 1.5491640947794878 0.0 40 0.0 0.0 0.0 2.010595840077207 0.0 41 0.0 0.0 0.0 2.587636846179365 0.0 42 0.0 0.0 0.0 3.117428850039709 0.0 43 0.0 0.0 0.0 3.7155811124626785 0.0 44 0.0 0.0 0.0 4.296643310244991 0.0 45 0.0 0.0 0.0 4.884742593467574 0.0 46 0.0 0.0 0.0 5.483900153810581 0.0 47 0.0 0.0 0.0 6.139354397675751 0.0 48 0.0 0.0 0.0 6.750575533059222 0.0 49 0.0 0.0 0.0 7.372854945563118 0.0 50 0.0 0.0 0.0 8.015240316467786 0.0 51 0.0 0.0 0.0 8.625456153931218 0.0 52 0.0 0.0 0.0 9.27186271651604 0.0 53 0.0 0.0 0.0 9.83281895589758 0.0 54 0.0 0.0 0.0 10.363616257677963 0.0 55 0.0 0.0 0.0 10.947694349220392 0.0 56 0.0 0.0 0.0 11.576005549244519 0.0 57 0.0 0.0 0.0 12.070612125903512 0.0 58 0.0 0.0 0.0 12.579292873443045 0.0 59 0.0 0.0 0.0 13.176439837945976 0.0 60 0.0 0.0 0.0 13.704221245966242 0.0 61 0.0 0.0 0.0 14.268193379107899 0.0 62 0.0 0.0 0.0 14.819096639289054 0.0 63 0.0 0.0 0.0 15.342856855629165 0.0 64 0.0 0.0 0.0 15.927940245091634 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTTGC 15 0.0022196756 69.993965 2 GAGACAT 15 0.0022196756 69.993965 19 AAGGCAC 15 0.0022196756 69.993965 18 AGGCGTC 20 0.006935835 52.495476 18 CAAGGCA 20 0.006935835 52.495476 17 CGCAGAA 20 0.006935835 52.495476 58 GACATAC 20 0.006935835 52.495476 21 CCCGGCC 20 0.006935835 52.495476 22 TGGTCTT 20 0.006935835 52.495476 55 CAAACCG 30 5.8558804E-4 46.662643 2 CCGACAA 30 5.8558804E-4 46.662643 21 GTCTACC 35 0.0012507765 39.99655 39 AGTCTAC 35 0.0012507765 39.99655 38 CCCTTAG 35 0.0012507765 39.99655 48 TCCCGAC 35 0.0012507765 39.99655 19 AACAGTC 35 0.0012507765 39.99655 35 CTTGCCC 40 0.0024098766 34.996983 25 GAACAGT 40 0.0024098766 34.996983 34 AACAGGT 40 0.0024098766 34.996983 26 TCCTTGC 45 0.0042915535 31.10843 23 >>END_MODULE