FastQCFastQC Report
Wed 25 May 2016
SRR1780406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99473
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT35623.580871191177505No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT35013.5195480180551506Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC13411.3481045107717673No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC6630.6665125209855941No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG3870.38905029505493954No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT3070.30862646145185124No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1710.17190594432660117No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1700.17090064640656258No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT1480.1487840921657133No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1400.1407417088054045No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1330.13370462336513425No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1280.12867813376494125No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC1160.11661455872447801No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT1150.1156092608044394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTG207.8931145E-570.029
CTTATAA207.8931145E-570.01
CACGTTT207.8931145E-570.028
TTTACAC1500.060.6666682
CTATACA1100.054.0909081
CAGGTGT200.00693347252.514
AACCGTT200.00693347252.524
CGTATGA200.00693347252.538
AAAAGTT200.00693347252.513
GAATCTC200.00693347252.532
TATGACG200.00693347252.540
AATCTCG200.00693347252.533
GTATAGA200.00693347252.51
GAGCACT200.00693347252.561
ACCGTTT200.00693347252.525
GAACCGT200.00693347252.523
CCTATGC200.00693347252.538
TCTTTAT552.25009E-950.909091
CTTTATA552.25009E-950.909092
CTTTACA2000.047.2499961
Produced by FastQC (version 0.11.5)