##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780406_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99473 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.90311943944588 34.0 34.0 34.0 31.0 34.0 2 33.289777125451124 34.0 34.0 34.0 33.0 34.0 3 33.49555155670383 34.0 34.0 34.0 34.0 34.0 4 33.52876659998191 34.0 34.0 34.0 34.0 34.0 5 33.55564826636374 34.0 34.0 34.0 34.0 34.0 6 37.29106390678878 38.0 38.0 38.0 36.0 38.0 7 36.96313572527218 38.0 37.0 38.0 35.0 38.0 8 36.96268334120817 38.0 37.0 38.0 35.0 38.0 9 36.73593839534346 38.0 37.0 38.0 34.0 38.0 10-11 36.48561921325385 38.0 37.0 38.0 34.0 38.0 12-13 36.35009500065344 38.0 37.0 38.0 34.0 38.0 14-15 36.550028650990726 38.0 37.0 38.0 34.0 38.0 16-17 36.63332763664512 38.0 37.0 38.0 34.0 38.0 18-19 36.679551235008496 38.0 37.0 38.0 34.0 38.0 20-21 36.4453871904939 38.0 37.0 38.0 34.0 38.0 22-23 36.48498084907462 38.0 37.0 38.0 34.0 38.0 24-25 35.7760447558634 38.0 36.5 38.0 32.0 38.0 26-27 36.01472761452857 38.0 37.0 38.0 33.0 38.0 28-29 35.813280990821625 38.0 36.5 38.0 31.5 38.0 30-31 35.50032672182401 38.0 36.0 38.0 31.0 38.0 32-33 35.91502719330874 38.0 36.5 38.0 32.5 38.0 34-35 35.91116182280619 38.0 37.0 38.0 32.5 38.0 36-37 36.007067244377865 38.0 37.0 38.0 33.5 38.0 38-39 35.553220471886846 38.0 36.5 38.0 29.5 38.0 40-41 35.37566475324962 38.0 36.0 38.0 29.0 38.0 42-43 35.88409920279875 38.0 36.5 38.0 32.5 38.0 44-45 36.0781769927518 38.0 37.0 38.0 33.5 38.0 46-47 36.32116755300433 38.0 37.0 38.0 34.0 38.0 48-49 36.074185960009245 38.0 37.0 38.0 33.0 38.0 50-51 36.190101836679304 38.0 37.0 38.0 34.0 38.0 52-53 36.08956199169624 38.0 37.0 38.0 34.0 38.0 54-55 36.275150040714564 38.0 37.0 38.0 34.0 38.0 56-57 36.285580006634966 38.0 37.0 38.0 34.0 38.0 58-59 36.30712856755099 38.0 37.0 38.0 34.0 38.0 60-61 36.04631407517618 38.0 37.0 38.0 33.0 38.0 62-63 36.36600384023805 38.0 37.0 38.0 34.0 38.0 64-65 36.335779558272094 38.0 37.0 38.0 34.0 38.0 66-67 35.573733575945234 38.0 36.5 38.0 31.0 38.0 68-69 34.444844329617084 37.5 34.5 38.0 24.0 38.0 70-71 33.83772480974737 37.0 34.0 38.0 22.0 38.0 72-73 33.127763312657706 37.0 34.0 38.0 20.0 38.0 74-75 33.52018638223437 38.0 34.0 38.0 20.5 38.0 76 33.115659525700444 37.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2962097802839736 1101 2 0.4745999001026533 1101 3 0.10419406859571012 1101 4 0.01723153812341849 1101 5 0.258258325492271 1101 6 -1.1211504245953137 1101 7 -0.014668418030936436 1101 8 0.5412407559946715 1101 9 0.1198476054319002 1101 10-11 -0.04807720553618822 1101 12-13 0.6218136492599058 1101 14-15 0.5240167480494122 1101 16-17 -0.07290413059744338 1101 18-19 -0.6547674808075286 1101 20-21 0.16969539667772437 1101 22-23 0.6384782361434205 1101 24-25 -1.4267610841171887 1101 26-27 -0.4025489850839179 1101 28-29 0.7155064218521261 1101 30-31 1.1490712522723427 1101 32-33 0.40913808439005095 1101 34-35 0.6844350908275132 1101 36-37 0.5579261654956369 1101 38-39 0.36379448617198307 1101 40-41 1.3401954363341844 1101 42-43 0.7605221853146631 1101 44-45 0.42531422243738604 1101 46-47 0.6032505256018297 1101 48-49 -1.4377698106315222 1101 50-51 -1.2523944835532177 1101 52-53 -1.4949061535613524 1101 54-55 -1.0284209319514446 1101 56-57 -0.730753814405837 1101 58-59 -1.1898947068070882 1101 60-61 -1.3476003134358265 1101 62-63 -0.23633353510700772 1101 64-65 -1.1567836865826777 1101 66-67 -1.4673640100577856 1101 68-69 -1.546853138206174 1101 70-71 -0.163920385812375 1101 72-73 -1.013103244613795 1101 74-75 -0.735712539481419 1101 76 -0.9099895806547096 1102 1 -0.6017852589884569 1102 2 0.1945943881831269 1102 3 0.24605709739548587 1102 4 0.34019557284850777 1102 5 0.03447439273768538 1102 6 0.16887989096207434 1102 7 -0.1741695893138342 1102 8 0.0852672132083967 1102 9 0.43326912947765095 1102 10-11 0.6114067409981985 1102 12-13 0.6993422423424391 1102 14-15 0.1522894502679577 1102 16-17 0.05242313962440903 1102 18-19 -0.2894305647265085 1102 20-21 0.4021089284401569 1102 22-23 0.5674950474979781 1102 24-25 -4.561148572059864 1102 26-27 -0.9361716941923675 1102 28-29 0.05891623306888505 1102 30-31 1.4588239048836158 1102 32-33 0.4943489153119174 1102 34-35 0.7973260926188814 1102 36-37 -2.2119511942949117 1102 38-39 -0.0297565679826306 1102 40-41 0.7507645420252373 1102 42-43 0.8541731430106765 1102 44-45 0.8958254529734191 1102 46-47 1.028546791276348 1102 48-49 -3.1979496370060616 1102 50-51 -0.48601374771195793 1102 52-53 0.011897622103525407 1102 54-55 0.30815065410340026 1102 56-57 0.6815205413885579 1102 58-59 0.01649222994363697 1102 60-61 -5.845533343613589 1102 62-63 -2.6103861739384797 1102 64-65 0.14799101370669376 1102 66-67 0.9424816561954827 1102 68-69 -1.0125414391569763 1102 70-71 -1.4320063717569838 1102 72-73 -5.9905215993058185 1102 74-75 -2.2993235357608697 1102 76 -0.7473535051414117 1103 1 0.8076594737567433 1103 2 0.4346912572369206 1103 3 0.10749975437524739 1103 4 0.4089553029990043 1103 5 0.26523031731821334 1103 6 0.03983647286832337 1103 7 0.7530921301118667 1103 8 0.40372422756577464 1103 9 0.2619319905743467 1103 10-11 -0.026545170435817056 1103 12-13 -0.9114084928244779 1103 14-15 -0.3254393163814058 1103 16-17 -0.07921498526747683 1103 18-19 0.5204639171970413 1103 20-21 -0.13456292764010414 1103 22-23 0.355766972770418 1103 24-25 -3.0805656279325433 1103 26-27 -0.6431428064566802 1103 28-29 0.06267855792617638 1103 30-31 1.1019652299138798 1103 32-33 0.9524876456353866 1103 34-35 0.5083622455336041 1103 36-37 -1.068290927905018 1103 38-39 -0.2870649921306949 1103 40-41 -0.48959179770837125 1103 42-43 -0.35729546103706156 1103 44-45 0.8431529049713404 1103 46-47 0.7864606470106494 1103 48-49 -2.265498503984091 1103 50-51 -0.5238964669646862 1103 52-53 0.19197027389386534 1103 54-55 -0.2806748086193167 1103 56-57 0.12573434522872873 1103 58-59 -0.507811523732201 1103 60-61 -4.0857599480072935 1103 62-63 -1.314978804971176 1103 64-65 -0.013226768683892942 1103 66-67 -0.16991239327692398 1103 68-69 0.3560799066309528 1103 70-71 -0.3003250017517871 1103 72-73 -5.041637323229015 1103 74-75 -2.86579127490635 1103 76 -3.0275818393396463 1104 1 0.26437657952524063 1104 2 0.4445870505646212 1104 3 0.20882602111360882 1104 4 -0.16601205240463912 1104 5 -0.22552678128127468 1104 6 0.013234327286220093 1104 7 0.3684619741859123 1104 8 0.4863854662681817 1104 9 0.8545658334194215 1104 10-11 -0.39119811357020495 1104 12-13 0.3324466419784926 1104 14-15 0.598658796632904 1104 16-17 0.2765727096399644 1104 18-19 0.6502101854716145 1104 20-21 -0.04650572918656337 1104 22-23 0.1601618315665334 1104 24-25 0.07651248866626759 1104 26-27 0.8530038509926854 1104 28-29 1.2350484276038287 1104 30-31 0.6278312718525925 1104 32-33 0.4795122506997842 1104 34-35 -0.025088864382674103 1104 36-37 0.9761633312546749 1104 38-39 1.1033771821274314 1104 40-41 1.5179932702692014 1104 42-43 0.38509386738038387 1104 44-45 0.6724536705191255 1104 46-47 0.8473076142636415 1104 48-49 0.047185521926756735 1104 50-51 0.7030986939539687 1104 52-53 0.4766183523433156 1104 54-55 0.4227202294020387 1104 56-57 0.3037946161148639 1104 58-59 -0.5185522360244903 1104 60-61 0.5832110431082569 1104 62-63 0.5229823799636648 1104 64-65 0.6744284531713447 1104 66-67 0.3168249393395044 1104 68-69 1.364362672844436 1104 70-71 0.5220307859431159 1104 72-73 0.2853655187711439 1104 74-75 -0.7063192212411948 1104 76 -0.7192504262766874 1105 1 0.6951869865960703 1105 2 0.37267120343403803 1105 3 0.25954181844959834 1105 4 -0.2619911386737783 1105 5 -0.10544710992561335 1105 6 0.07566430363378629 1105 7 0.3305682856511183 1105 8 -0.48950149999598125 1105 9 0.44036192454353085 1105 10-11 0.14419339645095164 1105 12-13 -0.08650020228890298 1105 14-15 0.31400798124636964 1105 16-17 0.1929053375498384 1105 18-19 0.6411413444408609 1105 20-21 0.2577579332060651 1105 22-23 -0.014604756258684404 1105 24-25 0.46385843663091464 1105 26-27 -0.12621935328964895 1105 28-29 0.908215363991232 1105 30-31 1.0517451271992897 1105 32-33 0.7415314216197402 1105 34-35 -1.247066954759866 1105 36-37 0.2551500111999019 1105 38-39 -0.1572166184452044 1105 40-41 0.5488161259893545 1105 42-43 0.2695081584298009 1105 44-45 0.5878215281065806 1105 46-47 0.6672627991260853 1105 48-49 0.14111680538250226 1105 50-51 0.6609164457278993 1105 52-53 0.3603977622773371 1105 54-55 0.6133090505149426 1105 56-57 0.4663677519296314 1105 58-59 0.08032738553657026 1105 60-61 0.4342816269499039 1105 62-63 -0.7345801744991789 1105 64-65 0.25274290605320715 1105 66-67 0.26483937977271665 1105 68-69 0.5213525894384787 1105 70-71 0.8084057392606852 1105 72-73 0.4734397127210954 1105 74-75 -0.255715461749098 1105 76 0.4977544286965525 1106 1 -1.1580340772905906 1106 2 0.09344105106836054 1106 3 0.35061372435710325 1106 4 0.020286327244427582 1106 5 0.27100303709389806 1106 6 0.058556578426625094 1106 7 -0.9228933556212695 1106 8 0.19871020810346351 1106 9 -0.6653317402519789 1106 10-11 0.07794105750414815 1106 12-13 1.0259162638966117 1106 14-15 -0.702645320009978 1106 16-17 0.13107694941832193 1106 18-19 0.08252065478568937 1106 20-21 0.12970393694838833 1106 22-23 0.3198111753104129 1106 24-25 -0.7148638301426757 1106 26-27 0.776533921235881 1106 28-29 0.5292792506525501 1106 30-31 0.37790123519179986 1106 32-33 -0.15397780051337406 1106 34-35 0.5906235023371593 1106 36-37 0.7499508665861612 1106 38-39 0.10453157722440665 1106 40-41 0.7743039634659681 1106 42-43 0.7034830314584042 1106 44-45 0.9560920440114202 1106 46-47 0.7911200565438818 1106 48-49 0.588163049702203 1106 50-51 0.10483518403317049 1106 52-53 0.36820316190310365 1106 54-55 -0.19062038011857396 1106 56-57 -0.06167287891774009 1106 58-59 0.4081541698188502 1106 60-61 0.9106114852765543 1106 62-63 0.5902661232821629 1106 64-65 -1.0825818734175243 1106 66-67 0.20127348829985436 1106 68-69 1.2483774491016746 1106 70-71 0.1663581583999445 1106 72-73 0.06449825321828939 1106 74-75 -0.31449653632808605 1106 76 -0.7951886346554957 1107 1 0.8208095919600567 1107 2 -0.13992458200657154 1107 3 0.11902457707028447 1107 4 0.27464679236070566 1107 5 0.00872913528511532 1107 6 0.28651200478321215 1107 7 0.7282694350764061 1107 8 0.17012493678563345 1107 9 -0.40887179193155987 1107 10-11 0.48043621254290514 1107 12-13 -0.07966351646514624 1107 14-15 0.2503585559590107 1107 16-17 0.6271606057490686 1107 18-19 0.6293956547856894 1107 20-21 0.8388043891447694 1107 22-23 0.24576401768767653 1107 24-25 0.5923067124929844 1107 26-27 0.07797125973716845 1107 28-29 0.6196377777843338 1107 30-31 0.6894726176207442 1107 32-33 0.9181717472902378 1107 34-35 0.2941926237841912 1107 36-37 1.3283181146481766 1107 38-39 0.7932024428574778 1107 40-41 0.6839454363341844 1107 42-43 1.022321855747812 1107 44-45 0.7297516047349362 1107 46-47 0.6999136611950476 1107 48-49 0.8875009049993565 1107 50-51 1.0505716181416958 1107 52-53 0.833925061127907 1107 54-55 0.8224206405532613 1107 56-57 0.7063342270254154 1107 58-59 0.819168381705154 1107 60-61 1.2553304775246232 1107 62-63 1.0005132163054213 1107 64-65 0.8226183849804087 1107 66-67 -0.03168839800505907 1107 68-69 0.847214658403999 1107 70-71 0.2470671377281164 1107 72-73 0.5708774521847033 1107 74-75 -0.08387638128932196 1107 76 0.7554412103057473 1108 1 -0.38557138866469387 1108 2 0.3305924207613202 1108 3 -0.029107095527578508 1108 4 0.31979803791230665 1108 5 -0.005901153366529854 1108 6 -0.41095982202580217 1108 7 -0.3547673383518273 1108 8 -0.5344272577576632 1108 9 -0.2112640418327132 1108 10-11 0.46455172262594857 1108 12-13 1.102504583574401 1108 14-15 0.671696041131014 1108 16-17 -0.27668602335334214 1108 18-19 0.3343000142162964 1108 20-21 0.8664277086386392 1108 22-23 -0.048633471438456866 1108 24-25 1.3952426555534885 1108 26-27 -0.4745074868029633 1108 28-29 -0.7919503005074802 1108 30-31 0.5846448694989519 1108 32-33 0.7919793293384743 1108 34-35 0.6967850516212835 1108 36-37 1.3016833281713076 1108 38-39 1.220872717669664 1108 40-41 0.9780957922060693 1108 42-43 0.22999410762601968 1108 44-45 0.7468655825917949 1108 46-47 0.8810190882413096 1108 48-49 0.8815573502346297 1108 50-51 0.6866926145829737 1108 52-53 -0.06329839676057958 1108 54-55 -0.5619271747887709 1108 56-57 0.37827718867033155 1108 58-59 0.5110673536308212 1108 60-61 1.2094749021984015 1108 62-63 1.080121015830926 1108 64-65 0.8276846166925509 1108 66-67 0.9274728317300145 1108 68-69 1.485115014275884 1108 70-71 0.20522129945211987 1108 72-73 0.015358966617284864 1108 74-75 -0.33862133977093833 1108 76 0.7912137488585316 1109 1 -0.710794716429966 1109 2 0.41095410280068023 1109 3 0.3171538287709623 1109 4 0.2750719624287328 1109 5 0.099432083130921 1109 6 -0.21157472991066584 1109 7 -0.0506421295494448 1109 8 0.4154480660373352 1109 9 0.5327381853927022 1109 10-11 0.33237073635243775 1109 12-13 0.7946632975938144 1109 14-15 0.8451714811290856 1109 16-17 0.859126308696915 1109 18-19 0.5638131717924963 1109 20-21 0.9595172576254924 1109 22-23 0.28821016281239764 1109 24-25 -0.19765927390157856 1109 26-27 0.18461808513399092 1109 28-29 -0.8376326303789341 1109 30-31 0.46048310514908763 1109 32-33 0.6522633005782197 1109 34-35 0.09046529952115634 1109 36-37 1.1152016180495323 1109 38-39 1.302166445125053 1109 40-41 0.6327124431369029 1109 42-43 0.9119547922557487 1109 44-45 0.6702986568891234 1109 46-47 0.654314171399129 1109 48-49 0.600581970758995 1109 50-51 0.18662603991040072 1109 52-53 -0.2680094626816114 1109 54-55 -0.40862386058052635 1109 56-57 -0.05649173669340257 1109 58-59 0.1702171345396266 1109 60-61 0.9668525863681552 1109 62-63 0.4816994407952109 1109 64-65 0.7757079541414029 1109 66-67 0.7386489035822379 1109 68-69 0.22306499854005324 1109 70-71 0.6751779653925141 1109 72-73 0.47982019594887504 1109 74-75 0.2868719180303998 1109 76 0.7139871286730894 1110 1 -0.7448331493213729 1110 2 -0.401126933993595 1110 3 0.07066691283670679 1110 4 -0.07069992296776206 1110 5 0.1466042366638618 1110 6 -0.12582072027761626 1110 7 0.42656627205937525 1110 8 0.4403499976128842 1110 9 0.6387762210449139 1110 10-11 -0.17440715728677247 1110 12-13 0.3852214186524563 1110 14-15 0.7860945656913714 1110 16-17 0.6309496365682179 1110 18-19 0.19383452339882723 1110 20-21 0.997652219639491 1110 22-23 -0.3288760877462238 1110 24-25 -0.6513064261931447 1110 26-27 1.037850619023466 1110 28-29 0.15316879968214892 1110 30-31 -0.06203539130058999 1110 32-33 0.6027697805424737 1110 34-35 0.8444004502237732 1110 36-37 1.25543946501314 1110 38-39 0.5554312546985187 1110 40-41 -0.5758629578264021 1110 42-43 0.3296642117899822 1110 44-45 0.8542700556676195 1110 46-47 0.8359766173994316 1110 48-49 1.3242152196790045 1110 50-51 -0.4092823964568453 1110 52-53 0.4407149029770565 1110 54-55 -0.30350444054974446 1110 56-57 0.7652099366766763 1110 58-59 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44-45 -0.5157957808859877 2116 46-47 -0.5766182015500547 2116 48-49 -0.37446804924900334 2116 50-51 -1.605310734799481 2116 52-53 -0.3873371937740515 2116 54-55 -0.26248540519837604 2116 56-57 -0.10351054421641237 2116 58-59 -2.224540768621644 2116 60-61 -1.9016548165930303 2116 62-63 0.14824605944266978 2116 64-65 -1.95638488299344 2116 66-67 -0.833199835913554 2116 68-69 -1.1812167141450232 2116 70-71 0.48430161485229917 2116 72-73 0.21299346525659502 2116 74-75 0.342594206945968 2116 76 -0.4895342798903357 2117 1 0.6176285883758297 2117 2 0.5090449520436024 2117 3 0.26821812545738055 2117 4 -0.27575643344574274 2117 5 -0.0831167428510895 2117 6 0.1853695316649322 2117 7 0.5978930909903895 2117 8 0.2541303131297141 2117 9 -0.5885312901394499 2117 10-11 -0.2465463143388078 2117 12-13 -1.375418444780557 2117 14-15 -0.06281348705174139 2117 16-17 -0.8338048960430413 2117 18-19 -1.0545761194078622 2117 20-21 -0.2980123671276331 2117 22-23 0.08301760191707075 2117 24-25 -1.270596513313464 2117 26-27 -2.4389418227001016 2117 28-29 -1.5386283512479224 2117 30-31 -0.42415865478069037 2117 32-33 -1.9608045071900477 2117 34-35 0.39219889618562576 2117 36-37 -1.3959762401905351 2117 38-39 0.4683256507427842 2117 40-41 -0.21625617656904694 2117 42-43 0.25344864786250554 2117 44-45 -0.3622062805697297 2117 46-47 -0.7421226291275289 2117 48-49 -0.8926290233160543 2117 50-51 -0.5542670915357277 2117 52-53 0.2934344697300659 2117 54-55 -0.33545346338939197 2117 56-57 0.09667920910427341 2117 58-59 -0.9035018691909045 2117 60-61 -0.7584464041958086 2117 62-63 -0.6686736116515704 2117 64-65 -1.4544873497866107 2117 66-67 -0.8718541686143837 2117 68-69 -0.15076921256374476 2117 70-71 -0.40117434972708566 2117 72-73 0.8597439396398983 2117 74-75 0.5821182423665832 2117 76 0.5924707801982194 2118 1 -0.10979665297100638 2118 2 0.1134716061523875 2118 3 -1.2286031601559912 2118 4 0.06798620841910008 2118 5 0.2779910979777327 2118 6 0.3668800096493925 2118 7 0.8170772209644852 2118 8 0.6155324793647097 2118 9 -1.3867712242105554 2118 10-11 -0.6269619018123223 2118 12-13 -0.8647785813475437 2118 14-15 0.7204011350344359 2118 16-17 -2.8854737210960195 2118 18-19 -0.31711438171065964 2118 20-21 -0.20855215992254728 2118 22-23 0.6911969049545021 2118 24-25 0.9454089022740106 2118 26-27 -2.274193176596974 2118 28-29 0.21703741282082945 2118 30-31 -0.5091156832713963 2118 32-33 0.23415664185633744 2118 34-35 -0.12092801692951127 2118 36-37 -2.5000349875416035 2118 38-39 -0.16537166500950917 2118 40-41 -0.28206733738844036 2118 42-43 0.4792992482863312 2118 44-45 -2.1256569516316475 2118 46-47 -0.1822715577830536 2118 48-49 0.2968429569052802 2118 50-51 -0.712202104486046 2118 52-53 -1.513664659066734 2118 54-55 -0.4896358274110497 2118 56-57 0.407545703100034 2118 58-59 -0.8073016101845383 2118 60-61 -0.7675557755167404 2118 62-63 -0.8435029029159935 2118 64-65 0.011841823747644753 2118 66-67 0.2878320805026462 2118 68-69 -0.9283327868514704 2118 70-71 -0.20372615099856262 2118 72-73 1.0135731131741537 2118 74-75 -0.02244085817497421 2118 76 -0.21498153908598283 2119 1 0.8483889570394183 2119 2 -1.0686377339340112 2119 3 -0.36311828007256963 2119 4 -0.031730758659705316 2119 5 -0.33866315496432264 2119 6 -0.3680373149446794 2119 7 -0.6322747826106649 2119 8 0.12633241977882648 2119 9 -0.6107992295732885 2119 10-11 0.3711462465565134 2119 12-13 -0.06384010376672933 2119 14-15 -0.3531278385988088 2119 16-17 -5.7364772473533776E-5 2119 18-19 0.07129616498976787 2119 20-21 -1.3884419260479746 2119 22-23 -0.3383204494325085 2119 24-25 0.8193475288195202 2119 26-27 0.8825772688074665 2119 28-29 0.3807347165598429 2119 30-31 0.8118436493667716 2119 32-33 0.34512044343536274 2119 34-35 0.30032924509938397 2119 36-37 -0.19228137514773636 2119 38-39 -1.08694920113345 2119 40-41 -0.6240902779515238 2119 42-43 0.4469888058611886 2119 44-45 0.12642110586872235 2119 46-47 0.12982437548075865 2119 48-49 -0.2228874169961017 2119 50-51 0.34342876099884023 2119 52-53 0.4694055033047846 2119 54-55 0.11654620744668875 2119 56-57 -0.6020699679859192 2119 58-59 -0.07604136886173762 2119 60-61 0.4491655115382258 2119 62-63 -0.24006926668778306 2119 64-65 0.2682249609441314 2119 66-67 0.7689210124257784 2119 68-69 1.1910922094244114 2119 70-71 0.4452459926034038 2119 72-73 0.6975072707788001 2119 74-75 0.10762021735012439 2119 76 0.49902114227853644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 1.0 20 0.0 21 5.0 22 10.0 23 24.0 24 65.0 25 136.0 26 250.0 27 423.0 28 747.0 29 1126.0 30 1867.0 31 2877.0 32 4354.0 33 6633.0 34 9872.0 35 13090.0 36 19980.0 37 38011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.09696098438772 22.46036612950248 10.917535411619234 31.525137474490567 2 17.343399716505985 18.70959958983845 41.602243824957526 22.34475686869804 3 16.05360248509646 25.819066480351456 34.84563650437808 23.281694530174015 4 11.672514149568226 22.205020457812672 36.586812501884936 29.53565289073417 5 11.42420556331869 38.7421712424477 36.21183637771053 13.621786816523077 6 23.385240215937994 38.45365073939662 17.692238094759382 20.468870949906005 7 23.161058779769384 34.909975571260546 20.65183517135303 21.277130477617042 8 20.252731897097707 34.98637821318348 23.088677329526604 21.672212560192214 9 19.949131925246046 19.06044856393192 20.037598142209447 40.95282136861259 10-11 21.052949041448432 25.31591487137213 29.9583806661104 23.672755421069034 12-13 23.237461421692316 20.612125903511505 27.497914006815922 28.652498667980257 14-15 23.05650779608537 23.33648326681612 22.1120304002091 31.494978536889406 16-17 24.119610346526194 27.41849547113287 22.343751570778 26.118142611562938 18-19 22.165813838931168 26.494626682617394 23.133413086968325 28.206146391483117 20-21 21.250992731696037 25.18120495008696 22.945422375921105 30.6223799422959 22-23 23.18619122777035 28.78067415278518 23.244498507132587 24.788636112311885 24-25 23.880852090517024 30.44997134900928 22.985634292722647 22.683542267751047 26-27 21.89790194324088 28.351411940928696 23.34402300121641 26.406663114614016 28-29 21.77575824595619 23.961275924120113 26.259386969328357 28.003578860595336 30-31 22.79814623063545 22.953967408241432 30.177535612678817 24.0703507484443 32-33 22.046183386446575 23.786354086033395 34.371638535079875 19.79582399244016 34-35 23.01378263448373 25.49636584801906 32.16098840891498 19.32886310858223 36-37 20.232123289736915 26.8776451901521 30.841032239904298 22.04919928020669 38-39 20.83630733968011 24.584057985584028 28.679641711821297 25.89999296291456 40-41 20.40603982990359 24.815779156152924 30.115207141636425 24.66297387230706 42-43 24.204558020769454 24.082414323484766 30.83952429302424 20.873503362721543 44-45 19.842570345721956 25.669779739225717 29.263719803363724 25.223930111688595 46-47 23.811486534034362 22.649362138469737 27.75577292330582 25.78337840419008 48-49 21.88382777236034 22.254280055894565 29.314487348325674 26.54740482341942 50-51 18.430629417027735 22.27388336533532 32.23789369979794 27.057593517839013 52-53 19.4087842932253 22.375921104219234 35.2160887879123 22.99920581464317 54-55 18.37684597830567 22.810209805675914 32.4791652006072 26.333779015411217 56-57 22.08539000532808 22.644335648869543 30.157932303238066 25.11234204256431 58-59 18.78600223176138 27.47680275049511 31.556804358971778 22.180390658771724 60-61 19.98280940556734 33.0808360057503 25.475757240658268 21.460597348024088 62-63 16.77289314688408 37.20356277582862 23.996461351321464 22.02708272596584 64-65 16.855830225287264 38.005790516019424 23.052989253365237 22.08539000532808 66-67 16.616569320318074 37.430760105757344 22.94089853528093 23.011772038643652 68-69 16.57887064831663 36.045962220904165 23.181164738170157 24.19400239260905 70-71 17.29062157570396 34.37917826948016 23.119841565047803 25.210358589768077 72-73 17.41326792194867 31.951383792586935 23.3098428719351 27.3255054135293 74-75 17.545464598433746 31.363284509364348 23.691856081549766 27.39939481065214 76 17.70028047811969 31.349210338483807 23.954238838679842 26.996270344716656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.5 12 1.0 13 0.5 14 1.0 15 1.0 16 2.0 17 4.0 18 7.5 19 13.5 20 21.0 21 26.0 22 29.0 23 53.5 24 102.0 25 129.0 26 176.5 27 295.0 28 416.5 29 467.0 30 562.5 31 778.0 32 1047.0 33 1196.0 34 1431.0 35 1922.5 36 2318.5 37 2458.0 38 2948.0 39 4070.5 40 5992.5 41 8340.5 42 9399.0 43 9388.5 44 8932.5 45 8093.5 46 7700.0 47 7245.0 48 6398.0 49 5609.0 50 5212.0 51 4827.0 52 3999.0 53 3338.5 54 3121.0 55 2906.0 56 2225.0 57 1595.0 58 1431.0 59 1201.5 60 866.0 61 680.5 62 601.0 63 472.0 64 309.0 65 251.0 66 201.0 67 175.0 68 153.0 69 114.5 70 69.0 71 40.0 72 31.0 73 18.0 74 10.0 75 6.0 76 3.0 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 1.0 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 99473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.563771073557646 #Duplication Level Percentage of deduplicated Percentage of total 1 62.28004956629491 25.263136730570107 2 16.520446096654275 13.402631869954662 3 7.722428748451053 9.397524956520865 4 4.064436183395291 6.594754355453239 5 2.3370508054522925 4.739979692982015 6 1.600991325898389 3.896534738069627 7 1.100371747211896 3.1244659354799795 8 0.8897149938042132 2.887215626350869 9 0.6394052044609666 2.3343017703296374 >10 2.7806691449814123 16.536145486714986 >50 0.029739776951672865 0.8836568717139325 >100 0.024783147459727387 1.8246157248700652 >500 0.0024783147459727386 0.6665125209855941 >1k 0.007434944237918216 8.448523720004424 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT 3562 3.580871191177505 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT 3501 3.5195480180551506 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 1341 1.3481045107717673 No Hit CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 663 0.6665125209855941 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 387 0.38905029505493954 No Hit TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT 307 0.30862646145185124 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 171 0.17190594432660117 No Hit CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 170 0.17090064640656258 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT 148 0.1487840921657133 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 140 0.1407417088054045 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 133 0.13370462336513425 No Hit CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 128 0.12867813376494125 No Hit CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 116 0.11661455872447801 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT 115 0.1156092608044394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004021191680154414 0.0 2 0.0 0.0 0.0 0.004021191680154414 0.0 3 0.0 0.0 0.0 0.004021191680154414 0.0 4 0.0 0.0 0.0 0.005026489600193017 0.0 5 0.0 0.0 0.0 0.007037085440270224 0.0 6 0.0 0.0 0.0 0.007037085440270224 0.0 7 0.0 0.0 0.0 0.007037085440270224 0.0 8 0.0 0.0 0.0 0.008042383360308828 0.0 9 0.0 0.0 0.0 0.014074170880540448 0.0 10 0.0 0.0 0.0 0.015079468800579051 0.0 11 0.0 0.0 0.0 0.01709006464065626 0.0 12 0.0 0.0 0.0 0.01709006464065626 0.0 13 0.0 0.0 0.0 0.019100660480733465 0.0 14 0.0 0.0 0.0 0.02010595840077207 0.0 15 0.0 0.0 0.0 0.02111125632081067 0.0 16 0.0 0.0 0.0 0.024127150080926484 0.0 17 0.0 0.0 0.0 0.025132448000965087 0.0 18 0.0 0.0 0.0 0.028148341761080897 0.0 19 0.0 0.0 0.0 0.03418012928131252 0.0 20 0.0 0.0 0.0 0.03820132096146693 0.0 21 0.0 0.0 0.0 0.04523840640173715 0.0 22 0.0 0.0 0.0 0.04724900224181436 0.0 23 0.0 0.0 0.0 0.04925959808189157 0.0 24 0.0 0.0 0.0 0.05227549184200738 0.0 25 0.0 0.0 0.0 0.05529138560212319 0.0 26 0.0 0.0 0.0 0.06333376896243202 0.0 27 0.0 0.0 0.0 0.07137615232274085 0.0 28 0.0 0.0 0.0 0.09148211072351291 0.0 29 0.0 0.0 0.0 0.12465694208478682 0.0 30 0.0 0.0 0.0 0.15079468800579052 0.0 31 0.0 0.0 0.0 0.2040754777678365 0.0 32 0.0 0.0 0.0 0.2312185216088788 0.0 33 0.0 0.0 0.0 0.3116423552119671 0.0 34 0.0 0.0 0.0 0.3940767846551326 0.0 35 0.0 0.0 0.0 0.4925959808189157 0.0 36 0.0 0.0 0.0 0.6403747750645904 0.0 37 0.0 0.0 0.0 0.8766197862736622 0.0 38 0.0 0.0 0.0 1.2435535270877525 0.0 39 0.0 0.0 0.0 1.6205402471022288 0.0 40 0.0 0.0 0.0 2.104088546640797 0.0 41 0.0 0.0 0.0 2.6962090215435346 0.0 42 0.0 0.0 0.0 3.267218240125461 0.0 43 0.0 0.0 0.0 3.9005559297497814 0.0 44 0.0 0.0 0.0 4.524845938093754 0.0 45 0.0 0.0 0.0 5.151146542277804 0.0 46 0.0 0.0 0.0 5.78046304022197 0.0 47 0.0 0.0 0.0 6.472108009208529 0.0 48 0.0 0.0 0.0 7.125551657233621 0.0 49 0.0 0.0 0.0 7.781005901098791 0.0 50 0.0 0.0 0.0 8.460587295044887 0.0 51 0.0 0.0 0.0 9.112020347229901 0.0 52 0.0 0.0 0.0 9.8046706141365 0.0 53 0.0 0.0 0.0 10.400812280719391 0.0 54 0.0 0.0 0.0 10.94869964714043 0.0 55 0.0 0.0 0.0 11.568968463804248 0.0 56 0.0 0.0 0.0 12.22241211182934 0.0 57 0.0 0.0 0.0 12.758235903209917 0.0 58 0.0 0.0 0.0 13.284006715390106 0.0 59 0.0 0.0 0.0 13.909302021654117 0.0 60 0.0 0.0 0.0 14.474279452715813 0.0 61 0.0 0.0 0.0 15.052325756738009 0.0 62 0.0 0.0 0.0 15.618308485719743 0.0 63 0.0 0.0 0.0 16.171222341740975 0.0 64 0.0 0.0 0.0 16.783448775044484 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTTTG 20 7.8931145E-5 70.0 29 CTTATAA 20 7.8931145E-5 70.0 1 CACGTTT 20 7.8931145E-5 70.0 28 TTTACAC 150 0.0 60.666668 2 CTATACA 110 0.0 54.090908 1 CAGGTGT 20 0.006933472 52.5 14 AACCGTT 20 0.006933472 52.5 24 CGTATGA 20 0.006933472 52.5 38 AAAAGTT 20 0.006933472 52.5 13 GAATCTC 20 0.006933472 52.5 32 TATGACG 20 0.006933472 52.5 40 AATCTCG 20 0.006933472 52.5 33 GTATAGA 20 0.006933472 52.5 1 GAGCACT 20 0.006933472 52.5 61 ACCGTTT 20 0.006933472 52.5 25 GAACCGT 20 0.006933472 52.5 23 CCTATGC 20 0.006933472 52.5 38 TCTTTAT 55 2.25009E-9 50.90909 1 CTTTATA 55 2.25009E-9 50.90909 2 CTTTACA 200 0.0 47.249996 1 >>END_MODULE