FastQCFastQC Report
Wed 25 May 2016
SRR1780405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916665
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCGT353.2430363E-431.3858324
ACTCGGT508.3050196E-526.4159740
TTGCGAT801.284352E-724.79885716
CCGAGTT450.001388165424.47394613
GACCTAT450.00140104124.4351941
CATCGTT1153.5470293E-1022.9340632
CCGTGTA1551.8189894E-1221.30436734
GGTACAA956.9201633E-720.82398430
ATTGCGA1201.39825715E-820.20647615
CGAGTTC550.00444175720.03180514
GAATACG550.00446430720.01428611
CGTGTAT550.00446571920.01319135
ATACACG550.004518239319.9728034
ATCGTAC909.9536755E-619.56952510
TCGTTGT1252.2688255E-819.37276834
CGATTAG1051.756378E-618.89643519
GCCGTTA951.5695263E-518.56288715
CGTTAAA951.5702664E-518.5618717
GTGTCAC951.6084268E-518.51020827
GGCATAC600.007426961318.3263951
Produced by FastQC (version 0.11.5)