FastQCFastQC Report
Wed 25 May 2016
SRR1780405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916665
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT443384.836881521602766No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT368464.019570944674445RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC127371.3894934354426098No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC70270.7665832119694763No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG48210.5259282289604164No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT35010.3819279671417584No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC26090.284618699306726No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG19170.209127652959369No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC18160.19810945110809294No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC13950.15218209487653614No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG13460.14683663061205568No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC11110.12120022036403702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT39000.042.5902744
GCCGTCT43100.038.5387643
CTTATAA1850.035.7131351
ACGTGGA250.00235361335.19659830
CTATACA10200.034.546041
TTTACAC15500.034.0667952
CTTTACA15650.033.4919971
TTACACA17550.030.3382153
GCGCGAC300.00574548129.33209816
TTATACA72750.029.2143272
CTTATAC77450.027.838111
CGCAATA407.0348004E-427.49734328
TATACAC82600.026.0768053
CTTTATA2900.025.7948282
CCAACGT801.309636E-724.74895722
TGCCGTC67250.024.69919242
CGTGCGC450.001398299624.4434136
ACGTTCC901.5132173E-824.4420817
TCCGTAT1001.7407729E-924.20294213
CCCAACG752.06412E-623.46567521
Produced by FastQC (version 0.11.5)