FastQCFastQC Report
Wed 25 May 2016
SRR1780404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60690
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCC150.0021651270.40051
TACTCCG150.002215453769.9942346
AGCCTGC150.002215453769.994236
GGTTGAC252.3739284E-455.99538425
AACCCGT200.006922743352.49567438
ATCCAGT400.002402441634.9971164
AGGTTGA500.007160366527.99769224
GAAGGCT900.00522307119.442847
ACATAAG1200.001322053317.49855870
CGACGAA3201.2878445E-915.31123870
GACGAAC2505.0731596E-613.99884570
CCGACGA4053.5235644E-812.09776869
GCCGACG4104.1802195E-811.95023468
TGCCGAC4702.2853783E-811.169291567
CTGCCGA4904.22242E-810.71340366
GCTGCCG5056.5743734E-810.39518365
CGCTGCC5703.6165147E-89.82375264
ACGCTGC6151.1507163E-68.53588263
GACGCTG6301.6263384E-68.33264662
TGACGCT6351.8214105E-68.26703561
Produced by FastQC (version 0.11.5)