##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780404_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60690 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.19752842313396 34.0 23.0 34.0 12.0 34.0 2 29.1638985005767 34.0 27.0 34.0 12.0 34.0 3 28.935360026363487 34.0 27.0 34.0 11.0 34.0 4 29.600082385895536 34.0 31.0 34.0 11.0 34.0 5 29.488943812819244 34.0 31.0 34.0 11.0 34.0 6 32.14354918437964 37.0 31.0 38.0 11.0 38.0 7 31.477706376668316 37.0 27.0 38.0 11.0 38.0 8 31.02064590542099 36.0 27.0 38.0 11.0 38.0 9 31.36551326412918 37.0 27.0 38.0 12.0 38.0 10-11 31.528283077937058 37.0 27.5 38.0 11.0 38.0 12-13 31.806648541769647 37.0 29.0 38.0 11.0 38.0 14-15 31.379980227385072 37.0 27.0 38.0 11.0 38.0 16-17 31.213000494315374 37.0 27.0 38.0 11.0 38.0 18-19 31.228711484593838 37.0 26.5 38.0 11.0 38.0 20-21 31.326495304003956 37.0 27.0 38.0 11.0 38.0 22-23 31.178299555116162 37.0 27.0 38.0 11.0 38.0 24-25 31.547165925193607 37.0 27.0 38.0 11.0 38.0 26-27 31.543821057834897 37.0 27.0 38.0 11.0 38.0 28-29 31.554926676552974 37.0 27.0 38.0 11.0 38.0 30-31 31.403765035425934 37.0 27.0 38.0 11.0 38.0 32-33 31.29225572581974 37.0 26.5 38.0 11.0 38.0 34-35 31.274048442906576 37.0 26.5 38.0 11.0 38.0 36-37 31.182888449497447 37.0 26.5 38.0 11.0 38.0 38-39 31.49401878398418 37.0 27.0 38.0 11.0 38.0 40-41 31.138919097050582 37.0 26.0 38.0 11.0 38.0 42-43 31.48959466139397 37.0 27.0 38.0 11.0 38.0 44-45 30.67327401548855 37.0 26.0 38.0 11.0 38.0 46-47 31.271716922062943 37.0 27.0 38.0 11.0 38.0 48-49 31.208576371725158 37.0 27.0 38.0 11.0 38.0 50-51 31.141679024550996 37.0 26.5 38.0 11.0 38.0 52-53 31.100255396276157 37.0 26.0 38.0 11.0 38.0 54-55 30.916996210248804 37.0 26.0 38.0 11.0 38.0 56-57 30.851524139067394 37.0 26.0 38.0 11.0 38.0 58-59 30.756640303180095 36.5 25.5 38.0 11.0 38.0 60-61 30.860965562695668 37.0 26.0 38.0 11.0 38.0 62-63 30.82916460701928 37.0 26.0 38.0 11.0 38.0 64-65 30.878208930631075 37.0 26.0 38.0 11.0 38.0 66-67 30.92154391168232 37.0 25.5 38.0 11.0 38.0 68-69 30.629864887131323 36.0 25.0 38.0 11.0 38.0 70-71 30.568701598286374 36.0 25.0 38.0 11.0 38.0 72-73 30.64434832756632 36.0 25.0 38.0 11.0 38.0 74-75 30.152743450321303 36.0 24.0 38.0 11.0 38.0 76 29.32529246992915 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.5311957542343748 1101 2 -0.4374906376970955 1101 3 0.18044626202945224 1101 4 -1.2173854139469285 1101 5 -0.4054750550692745 1101 6 -1.1706190027397163 1101 7 -0.8238470802311788 1101 8 1.4963854794362348 1101 9 -0.6375285392169054 1101 10-11 -0.852122993125846 1101 12-13 0.07351966353355266 1101 14-15 -0.08959097345104539 1101 16-17 -0.9980567104498022 1101 18-19 -0.07807061195577347 1101 20-21 -0.09596478001685327 1101 22-23 -0.4849619602846751 1101 24-25 -1.2184982408968175 1101 26-27 -0.6439210982717647 1101 28-29 -0.4995069682236384 1101 30-31 -1.3722561680341592 1101 32-33 -0.38706649962494666 1101 34-35 -0.7396510000206185 1101 36-37 0.17433095983808755 1101 38-39 -0.18694215901818012 1101 40-41 0.07842688660838348 1101 42-43 -0.7140765767205046 1101 44-45 -0.017165414735284656 1101 46-47 0.28899426573178744 1101 48-49 -0.6234470698449712 1101 50-51 -0.5249953351373691 1101 52-53 -0.06653924659342536 1101 54-55 -1.7003901752505968 1101 56-57 -1.1790486459777156 1101 58-59 -0.08543462098054277 1101 60-61 -0.2759009384787561 1101 62-63 -1.92666743447327 1101 64-65 -0.4111863861361087 1101 66-67 -0.8754908394018521 1101 68-69 -0.060695776966550596 1101 70-71 0.3311538670976617 1101 72-73 -0.4456490945813343 1101 74-75 0.06846860152674594 1101 76 -1.1649830378465467 1102 1 0.2669153812806684 1102 2 -0.6324390233768682 1102 3 -0.18199608367667608 1102 4 -0.09087542053599051 1102 5 -0.012151971388185245 1102 6 0.01888242489041403 1102 7 1.0767020082818917 1102 8 1.9754102982370227 1102 9 0.5879052407084195 1102 10-11 0.054048761761041675 1102 12-13 -3.519605748282828 1102 14-15 -0.7537245117774205 1102 16-17 -1.8787287947898008 1102 18-19 -0.04268734817365427 1102 20-21 0.301421558660941 1102 22-23 -0.31658945863740584 1102 24-25 0.043395016296241096 1102 26-27 -4.24025318700447 1102 28-29 0.012265489496545712 1102 30-31 0.3448973571140961 1102 32-33 -5.4852435257615575 1102 34-35 -1.0760665502073117 1102 36-37 0.4107904371058204 1102 38-39 0.37007519217883456 1102 40-41 0.6085389006650459 1102 42-43 0.6470479565542604 1102 44-45 -4.459664865646772 1102 46-47 0.2628137254286891 1102 48-49 0.35276641576891166 1102 50-51 -5.042082969664055 1102 52-53 -0.6232710717636429 1102 54-55 0.1331481511276138 1102 56-57 -1.5950600670187889 1102 58-59 0.8072734835659112 1102 60-61 0.04373446674856751 1102 62-63 -3.9216597472340844 1102 64-65 -0.3816673876735557 1102 66-67 -0.1566900487143954 1102 68-69 -6.650021496272501 1102 70-71 -0.35619513356124344 1102 72-73 -0.18114217606607141 1102 74-75 -5.508272009059677 1102 76 -1.7868587242071854 1103 1 1.0309133075138206 1103 2 0.1592214713646527 1103 3 -0.08282559041641946 1103 4 0.931973046357804 1103 5 0.8529050572001715 1103 6 0.08776110952972971 1103 7 1.1695771378923183 1103 8 2.0717663936302984 1103 9 1.5231370582169355 1103 10-11 1.8505233581171403 1103 12-13 -0.8037858697463172 1103 14-15 0.17287895437574008 1103 16-17 0.44977809307866323 1103 18-19 0.23951158531767902 1103 20-21 0.7775917556687943 1103 22-23 0.542524006034494 1103 24-25 0.9850703236581175 1103 26-27 0.45619919042109913 1103 28-29 0.3996710590417969 1103 30-31 0.8448047782368882 1103 32-33 -3.613409723361915 1103 34-35 -0.4967079366685745 1103 36-37 0.6672339269591774 1103 38-39 1.0464379532512673 1103 40-41 1.7485551945474356 1103 42-43 1.7121864545545549 1103 44-45 -3.836571990517161 1103 46-47 0.9561554525802229 1103 48-49 1.0420902196498147 1103 50-51 0.798922066626865 1103 52-53 0.23482402525902302 1103 54-55 0.7099145561046569 1103 56-57 1.3190267349364824 1103 58-59 0.933310366990586 1103 60-61 1.2141351481291025 1103 62-63 0.7405939929525509 1103 64-65 0.3936251615784059 1103 66-67 0.42416834263503844 1103 68-69 -1.480342930134153 1103 70-71 1.110624596849064 1103 72-73 1.4907582831251638 1103 74-75 -0.3638489606224091 1103 76 0.23950774002033626 1104 1 -0.21351073709382362 1104 2 0.6171978075539641 1104 3 0.5305975021988409 1104 4 0.5664419706064088 1104 5 0.29313353537234477 1104 6 0.8671708742516131 1104 7 1.8282198687504732 1104 8 1.6861414786296152 1104 9 -0.7641266436734497 1104 10-11 0.2711679929600628 1104 12-13 -0.5834676322922192 1104 14-15 0.3514455258431681 1104 16-17 -0.13056326419047792 1104 18-19 0.8212639312989154 1104 20-21 1.4240795837661722 1104 22-23 0.400660140945412 1104 24-25 0.5208040377883982 1104 26-27 0.804798401627405 1104 28-29 0.4761534471826998 1104 30-31 -0.13862035436204678 1104 32-33 -0.15839136350879457 1104 34-35 0.38888297860410503 1104 36-37 -0.3738037447394511 1104 38-39 0.3134208173883515 1104 40-41 0.7304333395222713 1104 42-43 0.6699927921038551 1104 44-45 -1.935919195739519 1104 46-47 0.4640093897487283 1104 48-49 1.1844980803869305 1104 50-51 1.0248332593373206 1104 52-53 -0.07266951479265771 1104 54-55 0.7204204720573273 1104 56-57 1.0133691801969142 1104 58-59 1.0466484602858408 1104 60-61 0.19766227991204843 1104 62-63 0.7090252455025343 1104 64-65 0.0013362998892993971 1104 66-67 0.3014803241391846 1104 68-69 -3.5663622419796575 1104 70-71 -0.12438152310197381 1104 72-73 0.3578193466366528 1104 74-75 -0.4516362583240294 1104 76 0.3727866737888661 1105 1 0.13992493542079742 1105 2 0.7998134375380133 1105 3 -0.557719882171174 1105 4 0.392332454391628 1105 5 0.4090625624855839 1105 6 0.388236796633322 1105 7 1.291670161148133 1105 8 -0.13410041398069694 1105 9 0.9293209905636566 1105 10-11 1.1986998908866973 1105 12-13 -0.5861590198520261 1105 14-15 0.2771997789000551 1105 16-17 1.1455365497382815 1105 18-19 0.6689513316178939 1105 20-21 -0.021826848982371416 1105 22-23 0.0569267544488703 1105 24-25 0.34335520737904446 1105 26-27 0.5815295286874793 1105 28-29 0.07118268694877727 1105 30-31 0.45936608901913445 1105 32-33 0.518791650845273 1105 34-35 0.7348591880671584 1105 36-37 0.94888033287884 1105 38-39 0.41509545853667973 1105 40-41 0.5803469493043067 1105 42-43 0.9102572790788699 1105 44-45 1.3336182843242739 1105 46-47 1.0818821967662693 1105 48-49 0.04261876087603156 1105 50-51 0.8300399313203073 1105 52-53 0.05769664682350495 1105 54-55 0.43328929183403986 1105 56-57 0.7086276863106882 1105 58-59 1.2059055044113087 1105 60-61 0.5338365223362871 1105 62-63 0.6331679887242245 1105 64-65 0.18240687405197775 1105 66-67 0.5799558690621005 1105 68-69 1.6550957590836113 1105 70-71 1.0949665630224246 1105 72-73 0.6039982408418645 1105 74-75 -0.7385846900093007 1105 76 -0.8466444798528165 1106 1 0.20478342598683597 1106 2 0.260362322615201 1106 3 -0.03477820121062791 1106 4 0.17992536462604747 1106 5 0.9071686230916427 1106 6 0.6829051785658535 1106 7 0.7177707900789514 1106 8 0.6802935254132443 1106 9 1.1169281946802947 1106 10-11 0.6372719320530784 1106 12-13 0.7104747708855399 1106 14-15 0.2134669315587132 1106 16-17 1.6912948401899683 1106 18-19 0.07118117724396456 1106 20-21 0.008047364854107286 1106 22-23 0.5245153765186252 1106 24-25 1.1576240753035698 1106 26-27 0.7482497974124662 1106 28-29 -0.5373078790889565 1106 30-31 -0.030378050489158426 1106 32-33 1.1948029430122311 1106 34-35 1.4131575013890476 1106 36-37 0.7287009446569961 1106 38-39 1.109349317884881 1106 40-41 1.0549395736610805 1106 42-43 1.3738436141274732 1106 44-45 0.9316743163425727 1106 46-47 0.7672202470807328 1106 48-49 1.0582705619051893 1106 50-51 1.1681081131384943 1106 52-53 0.3130747200081814 1106 54-55 0.3222079596442171 1106 56-57 1.2688585325085135 1106 58-59 -0.3217174229757731 1106 60-61 0.6850836635598441 1106 62-63 0.5948246496733383 1106 64-65 0.6353978689061783 1106 66-67 1.2214295683188148 1106 68-69 0.9585191438749163 1106 70-71 -0.03980687894441104 1106 72-73 0.6384107622826853 1106 74-75 -1.672975770626799 1106 76 -0.4128251545240573 1107 1 -2.0031761574753766 1107 2 -0.5153877547123571 1107 3 -0.3442946462049541 1107 4 -0.24527310944344194 1107 5 0.224108513802026 1107 6 -0.10934144479737995 1107 7 0.20287234694048095 1107 8 0.439856366943296 1107 9 -0.42580816989336867 1107 10-11 0.11154763057869488 1107 12-13 -0.5601716875122342 1107 14-15 -0.8674692474288079 1107 16-17 0.23186603309645903 1107 18-19 0.4401882913794424 1107 20-21 0.13254473571708303 1107 22-23 -0.37908293258540837 1107 24-25 -0.5161051751346157 1107 26-27 0.10802915610923591 1107 28-29 -0.7361943622315508 1107 30-31 0.22634944719101568 1107 32-33 0.7587786106068215 1107 34-35 0.6792674096270126 1107 36-37 -0.10494169891450511 1107 38-39 0.33225889619192017 1107 40-41 0.5359169940136965 1107 42-43 0.26336830739481343 1107 44-45 -0.006669842849099439 1107 46-47 0.20056389075534042 1107 48-49 0.05646625218253831 1107 50-51 0.6107310639581662 1107 52-53 1.1768751118011487 1107 54-55 0.32870347464576355 1107 56-57 0.7968511090627963 1107 58-59 0.21369859929456325 1107 60-61 0.030995613245380582 1107 62-63 -0.37754363571690774 1107 64-65 0.155348379268073 1107 66-67 1.0213058442235443 1107 68-69 0.5240413626603591 1107 70-71 0.09721755656440223 1107 72-73 0.012933908998842725 1107 74-75 0.7078882359718186 1107 76 -0.8189494972397995 1108 1 -1.3307671549746836 1108 2 -0.9061444463859694 1108 3 -1.4922987214110321 1108 4 -1.1158853165380052 1108 5 -0.26797261984620846 1108 6 0.10247665282233243 1108 7 0.24788485041366926 1108 8 0.3074121694810401 1108 9 -0.6542049467758382 1108 10-11 0.01068628020358986 1108 12-13 0.17433789848387704 1108 14-15 0.3865828040669754 1108 16-17 -1.3400984014687012 1108 18-19 -0.1779926848176494 1108 20-21 -0.04583814841739908 1108 22-23 -0.6676816174052647 1108 24-25 -1.3881066570277376 1108 26-27 -1.6175427620161678 1108 28-29 -0.8878568610738213 1108 30-31 -0.46407381853094165 1108 32-33 0.34963718243232833 1108 34-35 -0.6107205499913952 1108 36-37 -0.5271886193553676 1108 38-39 -0.7432250345295763 1108 40-41 -0.3418731328734026 1108 42-43 -0.3269345545112685 1108 44-45 -0.7719287110532278 1108 46-47 -0.1354553738551978 1108 48-49 -0.16276131102946323 1108 50-51 -0.35731561567506276 1108 52-53 -0.19508006093201757 1108 54-55 0.111143041192026 1108 56-57 0.2361861095258888 1108 58-59 -1.6706674283056948 1108 60-61 0.09596736465780964 1108 62-63 -1.0927886111730558 1108 64-65 -0.8074653652906001 1108 66-67 -0.5764064832748304 1108 68-69 0.20071080787662154 1108 70-71 0.36729257740352494 1108 72-73 -0.3783784925941909 1108 74-75 0.07150775991365421 1108 76 -0.43254586660166083 1109 1 -1.6639045443479894 1109 2 -1.033344733770445 1109 3 -1.0475385894754332 1109 4 -0.4523864105584501 1109 5 -0.5070895657330183 1109 6 -0.2028693515537512 1109 7 -1.4178674111062008 1109 8 -0.8014020429875259 1109 9 -0.25650402257349114 1109 10-11 -0.05369763932875671 1109 12-13 -0.20363800040903968 1109 14-15 -0.5700972521036256 1109 16-17 -0.580635941065708 1109 18-19 -0.5233765716926726 1109 20-21 -0.08396557730607057 1109 22-23 -0.37505564176763073 1109 24-25 0.23009653685881304 1109 26-27 0.02203465217670697 1109 28-29 0.2084659239429989 1109 30-31 -0.2727345415641551 1109 32-33 0.471514325569391 1109 34-35 0.25510682337981905 1109 36-37 -0.11301207384738277 1109 38-39 0.039767029740044535 1109 40-41 -0.16705846320226314 1109 42-43 -0.23454697735833463 1109 44-45 -0.6314368917135589 1109 46-47 -0.9504516182626297 1109 48-49 -0.6622551224660569 1109 50-51 0.42822372007491083 1109 52-53 0.09367602149507448 1109 54-55 -0.7657878232474111 1109 56-57 0.10091946245361783 1109 58-59 -0.1960584997925814 1109 60-61 0.1061401219158995 1109 62-63 -0.2599941237297969 1109 64-65 -0.09434365143409806 1109 66-67 -0.7984926333025903 1109 68-69 0.9399420544067816 1109 70-71 -0.18309767765019558 1109 72-73 -0.6466257738717474 1109 74-75 0.5581835706317868 1109 76 -0.585872015323126 1110 1 1.2053319121649828 1110 2 0.31619821555069905 1110 3 -0.03631396250944263 1110 4 0.8215079300787345 1110 5 0.3703469176652874 1110 6 1.105202969994842 1110 7 0.5998096960318549 1110 8 1.3799059285140203 1110 9 -0.9245373603877134 1110 10-11 -0.6611045488596048 1110 12-13 -0.7863440092812013 1110 14-15 -0.4172373246940282 1110 16-17 0.3596618140088985 1110 18-19 0.8586976318293082 1110 20-21 -0.1700826629358616 1110 22-23 -0.0010806451283009721 1110 24-25 -0.2562087461093263 1110 26-27 -1.0728705770207618 1110 28-29 -0.2078870804930837 1110 30-31 -0.1929859194375254 1110 32-33 0.8022879510566874 1110 34-35 0.026547877284912147 1110 36-37 0.5451409037033663 1110 38-39 0.5929495811582406 1110 40-41 0.35320635733813077 1110 42-43 0.04648878013595237 1110 44-45 0.6343582420409781 1110 46-47 0.5724345223476668 1110 48-49 0.41418324290562936 1110 50-51 -0.6952639798847606 1110 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0.8017840750876921 2116 34-35 0.5905788850368516 2116 36-37 1.5746757874242938 2116 38-39 1.475701519142735 2116 40-41 1.3873924038497591 2116 42-43 1.8439694002909874 2116 44-45 2.758466769172763 2116 46-47 1.5264655300996033 2116 48-49 1.9303460336032998 2116 50-51 0.5514414464718271 2116 52-53 2.492886040762894 2116 54-55 1.263402928197678 2116 56-57 1.1098019287349281 2116 58-59 0.8358297468355467 2116 60-61 0.18099561324537916 2116 62-63 1.9121831402393745 2116 64-65 2.1383538437489484 2116 66-67 1.7571528387590654 2116 68-69 2.06229272878058 2116 70-71 -1.39332889152303 2116 72-73 1.969983089326714 2116 74-75 -0.025554386978999588 2116 76 0.6488100656017295 2117 1 0.2910010943611958 2117 2 0.9512282478932228 2117 3 1.93573285714249 2117 4 0.44440951640246595 2117 5 0.507610124195395 2117 6 1.1133271036772712 2117 7 -1.0940914282558403 2117 8 -2.0128632074785884 2117 9 0.6650976311416237 2117 10-11 -0.02341780929392101 2117 12-13 0.9489478434833174 2117 14-15 0.6777303327832556 2117 16-17 1.0888124377575537 2117 18-19 1.3128405549773134 2117 20-21 -0.07315135229362824 2117 22-23 0.6290595058034754 2117 24-25 1.5832144789022244 2117 26-27 1.3656016238108997 2117 28-29 1.1556197730414972 2117 30-31 0.8018931811323178 2117 32-33 1.4393778940722406 2117 34-35 0.6698945583260318 2117 36-37 0.2608347278216421 2117 38-39 1.0181960003789392 2117 40-41 -0.5764486557528912 2117 42-43 -0.029650908759784045 2117 44-45 1.486237188598814 2117 46-47 -0.042496942306581076 2117 48-49 0.06120696075562293 2117 50-51 1.1979094376418047 2117 52-53 1.6241663939637796 2117 54-55 0.3074426632970173 2117 56-57 0.05666727089828427 2117 58-59 0.11209906250883606 2117 60-61 1.660134686093059 2117 62-63 -0.010156815610514514 2117 64-65 0.574358258759986 2117 66-67 -0.15578314358088718 2117 68-69 0.699158070943934 2117 70-71 1.574816803841209 2117 72-73 1.2895415882229564 2117 74-75 0.6610239794669148 2117 76 0.12430675434345062 2118 1 0.8274249354207974 2118 2 0.8003961881207644 2118 3 1.7461845467332537 2118 4 1.2198382818974558 2118 5 0.817425033348055 2118 6 0.936896470293 2118 7 0.9711573406353153 2118 8 -2.4479116027918835 2118 9 -0.027846841604173278 2118 10-11 0.42990618459299057 2118 12-13 1.1339750127820096 2118 14-15 0.7437644642147418 2118 16-17 0.5823809553326917 2118 18-19 0.8207578584244146 2118 20-21 -0.44490377205929477 2118 22-23 -0.13465249963038772 2118 24-25 0.11498982276365766 2118 26-27 0.7495802279881794 2118 28-29 0.4686185843846751 2118 30-31 -0.14975395760091104 2118 32-33 0.30601484431846515 2118 34-35 0.8044250388830072 2118 36-37 0.10134245409095755 2118 38-39 -0.014683096241874694 2118 40-41 -1.0244024679451122 2118 42-43 -0.09833829201670241 2118 44-45 0.638979589685583 2118 46-47 -0.7971242134901395 2118 48-49 -0.4827308894736184 2118 50-51 0.4469542669846476 2118 52-53 -0.6389088310319728 2118 54-55 0.2691721589669065 2118 56-57 1.3204429543759524 2118 58-59 1.113393849399646 2118 60-61 -0.20618387393410842 2118 62-63 0.6933369863932235 2118 64-65 -0.11472307932797676 2118 66-67 -0.9198984432922153 2118 68-69 0.6872927287805801 2118 70-71 0.7966711084769713 2118 72-73 0.7265215508651686 2118 74-75 0.9682917668671536 2118 76 0.8077844245760879 2119 1 1.6176347256305874 2119 2 1.6424707801953566 2119 3 1.6232241737728792 2119 4 0.43778699984617475 2119 5 1.4129961289191506 2119 6 1.8716284050249286 2119 7 -0.24853962906165705 2119 8 1.96467580979553 2119 9 1.3375377737804435 2119 10-11 0.6405705202573238 2119 12-13 1.493823497630494 2119 14-15 0.8198134152636953 2119 16-17 0.9189194168711552 2119 18-19 0.7283918910584504 2119 20-21 0.43097035381483195 2119 22-23 1.804741439763557 2119 24-25 1.7767031094769514 2119 26-27 2.0135662419741998 2119 28-29 1.5519519177180037 2119 30-31 1.7797332218862714 2119 32-33 1.7672619305655495 2119 34-35 2.105707090165051 2119 36-37 0.9189415216900265 2119 38-39 1.2560045494457732 2119 40-41 1.5362035926609465 2119 42-43 0.4203097266313165 2119 44-45 -0.016032065326072598 2119 46-47 0.8928524764865493 2119 48-49 0.970066313323585 2119 50-51 2.098178043208428 2119 52-53 0.1833522412290982 2119 54-55 0.39941691421166325 2119 56-57 2.2298252387582345 2119 58-59 2.1119369729427753 2119 60-61 1.6288977111474807 2119 62-63 0.8451435131997478 2119 64-65 1.9925030278981346 2119 66-67 1.75346985507608 2119 68-69 0.6870013534892045 2119 70-71 0.6777899895958512 2119 72-73 -0.8745856752420558 2119 74-75 0.9834432820186656 2119 76 1.9872716040632703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 18.0 13 123.0 14 507.0 15 1080.0 16 1591.0 17 2007.0 18 2143.0 19 1979.0 20 1747.0 21 1334.0 22 990.0 23 844.0 24 714.0 25 734.0 26 711.0 27 783.0 28 933.0 29 1134.0 30 1424.0 31 1681.0 32 2228.0 33 2881.0 34 4013.0 35 6073.0 36 10260.0 37 12755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.241507492656694 21.133772548498982 10.00514445974875 35.61957549909557 2 16.750700280112042 28.662053056516722 30.064260998517057 24.522985664854176 3 19.403526116328884 25.322128851540615 27.946943483275668 27.327401548854834 4 11.557093425605537 25.974625144175317 32.641291810841984 29.826989619377166 5 10.863404185203493 35.3534354918438 35.55116164112704 18.23199868182567 6 21.91464821222607 35.826330532212886 17.76569451309936 24.49332674246169 7 21.573570604712472 31.051244027022573 19.92750041192948 27.44768495633548 8 19.21403855659911 38.87296094908552 18.110067556434338 23.802932937881035 9 20.871642774756964 22.265612127203823 19.296424452133795 37.56632064590542 10-11 20.646729279947273 27.252430383918274 25.974625144175317 26.126215191959133 12-13 20.239742956005934 25.077442741802603 25.462184873949578 29.220629428241885 14-15 19.82781347833251 26.45411105618718 23.04663041687263 30.671445048607676 16-17 21.417037403196574 28.39347503707365 23.769154720711814 26.420332839017963 18-19 19.911847091777886 27.152743450321303 24.610314714120943 28.325094743779864 20-21 19.28736200362498 27.79617729444719 25.19855000823859 27.71791069368924 22-23 22.097544900313064 27.56137749217334 24.242049761080903 26.099027846432694 24-25 20.416872631405504 29.462020102158508 24.009721535673094 26.111385730762894 26-27 18.850716757291153 29.9349151425276 25.554457076948427 25.659911023232823 28-29 20.757950238919097 27.876091613115833 27.40566814961279 23.960289998352284 30-31 20.50667325753831 27.29939034437304 27.387543252595155 24.80639314549349 32-33 18.71807546548031 30.1178118306146 26.42857142857143 24.735541275333663 34-35 19.37139561707036 29.18108419838524 26.02652825836217 25.42099192618224 36-37 19.896193771626297 27.546548030977096 26.53814466963256 26.01911352776405 38-39 19.55264458724666 26.701268742791235 28.227879387048937 25.518207282913163 40-41 19.1382435327072 26.280276816608993 27.790410281759765 26.791069368924042 42-43 17.66024056681496 25.950733234470256 28.941341242379302 27.44768495633548 44-45 17.46663371230845 26.674905256220132 29.22886801779535 26.629593013676057 46-47 18.1323117482287 26.94101169879717 28.745262811006754 26.181413741967376 48-49 17.20299884659746 27.839841819080576 27.694842642939527 27.262316691382438 50-51 16.969846762234305 27.71461525786785 27.465809853353107 27.849728126544736 52-53 16.66584280771132 27.489701763058168 28.10512440270226 27.73933102652826 54-55 16.426099851705388 28.103476684791563 26.954193442082712 28.51623002142033 56-57 17.316691382435327 27.71955841159993 26.464821222606687 28.498928983358052 58-59 17.31339594661394 27.146152578678528 26.668314384577364 28.872137090130167 60-61 16.93442082715439 26.27615752183226 27.137090130169717 29.65233152084363 62-63 17.202174987642117 26.79601252265612 26.229197561377493 29.77261492832427 64-65 18.048278134783324 27.280441588400066 25.093096061954196 29.578184214862414 66-67 17.463338276487065 28.90838688416543 23.98994892074477 29.63832591860274 68-69 17.193112539133303 29.30631075959796 23.682649530400397 29.817927170868348 70-71 17.26066897347174 30.022244191794368 23.5384742132147 29.178612621519196 72-73 17.611632888449495 30.35508320975449 23.278958642280443 28.75432525951557 74-75 17.99472730268578 30.863404185203493 23.017795353435492 28.124073158675234 76 19.167902455099686 29.815455594002305 22.85549513923216 28.161146811665844 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.5 11 3.5 12 5.0 13 5.0 14 8.0 15 8.5 16 9.0 17 12.0 18 10.0 19 21.0 20 32.5 21 31.0 22 38.0 23 47.5 24 88.0 25 126.0 26 140.5 27 212.5 28 299.5 29 329.0 30 388.5 31 534.5 32 751.5 33 882.0 34 983.5 35 1268.5 36 1590.0 37 1728.0 38 1957.0 39 2418.0 40 2980.5 41 3488.5 42 3666.0 43 3829.5 44 4107.0 45 4359.0 46 4497.0 47 4470.5 48 4362.5 49 4054.0 50 3827.0 51 3563.0 52 3014.5 53 2545.5 54 2361.0 55 2159.0 56 1754.0 57 1350.0 58 1149.0 59 1022.5 60 782.5 61 566.0 62 463.0 63 400.0 64 297.5 65 209.0 66 146.5 67 133.0 68 108.0 69 76.0 70 54.0 71 39.0 72 37.0 73 25.5 74 14.0 75 12.0 76 14.5 77 15.0 78 10.0 79 7.0 80 6.0 81 4.5 82 5.0 83 6.0 84 4.5 85 3.0 86 3.5 87 4.0 88 2.5 89 2.5 90 3.0 91 1.5 92 1.0 93 1.5 94 3.0 95 2.5 96 1.0 97 2.0 98 3.5 99 5.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7101664195089801 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 60690.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.26083374526281 #Duplication Level Percentage of deduplicated Percentage of total 1 82.70883001214521 60.59317844784972 2 9.17862444334488 13.448673587081892 3 3.816742386757231 8.388531883341573 4 1.830776843146957 5.364969517218652 5 1.0121002204129368 3.7073652990608013 6 0.5060501102064684 2.2244191794364805 7 0.3756016373532455 1.9261822376009228 8 0.16418514686698754 0.9622672598451145 9 0.1259502496513877 0.8304498269896194 >10 0.2811389501147047 2.5539627615752183 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0016477179106936892 0.0 2 0.0 0.0 0.0 0.0016477179106936892 0.0 3 0.0 0.0 0.0 0.0016477179106936892 0.0 4 0.0 0.0 0.0 0.006590871642774757 0.0 5 0.0 0.0 0.0 0.013181743285549514 0.0 6 0.0 0.0 0.0 0.014829461196243203 0.0 7 0.0 0.0 0.0 0.014829461196243203 0.0 8 0.0 0.0 0.0 0.014829461196243203 0.0 9 0.0 0.0 0.0 0.018124897017630582 0.0 10 0.0 0.0 0.0 0.02142033283901796 0.0 11 0.0 0.0 0.0 0.02142033283901796 0.0 12 0.0 0.0 0.0 0.02306805074971165 0.0 13 0.0 0.0 0.0 0.02306805074971165 0.0 14 0.0 0.0 0.0 0.029658922392486405 0.0 15 0.0 0.0 0.0 0.03295435821387378 0.0 16 0.0 0.0 0.0 0.03789751194595485 0.0 17 0.0 0.0 0.0 0.03789751194595485 0.0 18 0.0 0.0 0.0 0.04778381941011699 0.0 19 0.0 0.0 0.0 0.04778381941011699 0.0 20 0.0 0.0 0.0 0.05437469105289174 0.0 21 0.0 0.0 0.0 0.05931784478497281 0.0 22 0.0 0.0 0.0 0.06590871642774757 0.0 23 0.0 0.0 0.0 0.0757950238919097 0.0 24 0.0 0.0 0.0 0.0757950238919097 0.0 25 0.0 0.0 0.0 0.07909045971329709 0.0 26 0.0 0.0 0.0 0.08897676717745921 0.0 27 0.0 0.0 0.0 0.11039710001647718 0.0 28 0.0 0.0 0.0 0.14170374031965727 0.0 29 0.0 0.0 0.0 0.16312407315867525 0.0 30 0.0 0.0 0.0 0.19607843137254902 0.0 31 0.0 0.0 0.0 0.23891909705058495 0.0 32 0.0 0.0 0.0 0.29988465974625145 0.0 33 0.0 0.0 0.0 0.39710001647717913 0.0 34 0.0 0.0 0.0 0.48113362992255726 0.0 35 0.0 0.0 0.0 0.5783489866534849 0.0 36 0.0 0.0 0.0 0.7711319822046465 0.0 37 0.0 0.0 0.0 1.0644257703081232 0.0 38 0.0 0.0 0.0 1.3676058658757622 0.0 39 0.0 0.0 0.0 1.792717086834734 0.0 40 0.0 0.0 0.0 2.227714615257868 0.0 41 0.0 0.0 0.0 2.926347009391992 0.0 42 0.0 0.0 0.0 3.7353765035425934 0.0 43 0.0 0.0 0.0 4.526281100675565 0.0 44 0.0 0.0 0.0 5.262811006755643 0.0 45 0.0 0.0 0.0 6.057011039710002 0.0 46 0.0 0.0 0.0 6.729279947273027 0.0 47 0.0 0.0 0.0 7.429560059317844 0.0 48 0.0 0.0 0.0 8.085351787773932 0.0 49 0.0 0.0 0.0 8.688416543087824 0.0 50 0.0 0.0 0.0 9.228868017795353 0.0 51 0.0 0.0 0.0 9.803921568627452 0.0 52 0.0 0.0 0.0 10.430054374691053 0.0 53 0.0 0.0 0.0 11.173175152413906 0.0 54 0.0 0.0 0.0 11.891580161476355 0.0 55 0.0 0.0 0.0 12.59021255561048 0.0 56 0.0 0.0 0.0 13.206459054209919 0.0 57 0.0 0.0 0.0 13.75679683638161 0.0 58 0.0 0.0 0.0 14.326907233481627 0.0 59 0.0 0.0 0.0 14.860767836546383 0.0 60 0.0 0.0 0.0 15.295765364969517 0.0 61 0.0 0.0 0.0 15.776898994892074 0.0 62 0.0 0.0 0.0 16.389850057670127 0.0 63 0.0 0.0 0.0 16.933596968199044 0.0 64 0.0 0.0 0.0 17.478991596638654 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGATCC 15 0.00216512 70.4005 1 TACTCCG 15 0.0022154537 69.99423 46 AGCCTGC 15 0.0022154537 69.99423 6 GGTTGAC 25 2.3739284E-4 55.995384 25 AACCCGT 20 0.0069227433 52.495674 38 ATCCAGT 40 0.0024024416 34.997116 4 AGGTTGA 50 0.0071603665 27.997692 24 GAAGGCT 90 0.005223071 19.44284 7 ACATAAG 120 0.0013220533 17.498558 70 CGACGAA 320 1.2878445E-9 15.311238 70 GACGAAC 250 5.0731596E-6 13.998845 70 CCGACGA 405 3.5235644E-8 12.097768 69 GCCGACG 410 4.1802195E-8 11.950234 68 TGCCGAC 470 2.2853783E-8 11.1692915 67 CTGCCGA 490 4.22242E-8 10.713403 66 GCTGCCG 505 6.5743734E-8 10.395183 65 CGCTGCC 570 3.6165147E-8 9.823752 64 ACGCTGC 615 1.1507163E-6 8.535882 63 GACGCTG 630 1.6263384E-6 8.332646 62 TGACGCT 635 1.8214105E-6 8.267035 61 >>END_MODULE