FastQCFastQC Report
Wed 25 May 2016
SRR1780404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60690
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT30104.959630911188005No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT28774.740484429065743TruSeq Adapter, Index 9 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC10421.7169220629428241RNA PCR Primer, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC5150.84857472400725TruSeq Adapter, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG3440.5668149612786291No Hit
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT2210.3641456582633053RNA PCR Primer, Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT1890.31141868512110726No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1470.2422145328719723No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1210.1993738671939364No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG1150.18948755972977427No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT1050.17301038062283738No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT1050.17301038062283738No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT1040.17136266271214368No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG1030.16971494480145No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC900.14829461196243204No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT890.14664689405173834No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT760.12522656121272038No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC730.12028340748063931TruSeq Adapter, Index 9 (95% over 21bp)
CTTATTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG610.10051079255231504No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTC150.00221472970.0000147
CTATACA800.061.251
CTTTACA1600.054.68751
TTTACAC1650.053.0303082
GGTCGGA200.00692048852.51
GGAGAGC200.00692048852.56
CGGTTTC200.00692048852.555
GTCCTCT200.00692048852.548
GCGCCTA200.00692048852.518
CCGTCCT200.00692048852.546
GGGGTTC200.00692048852.519
AGCGCCT200.00692048852.517
GTGTAAT200.00692048852.51
GCCGTCC200.00692048852.545
CCTCTGC200.00692048852.550
TTACACA1850.047.29733
TCGTTAC305.8352214E-446.66666813
ATATCGT404.5432782E-543.7537
GTCTTAG350.00124638940.01
GGCTGTG350.00124638940.07
Produced by FastQC (version 0.11.5)