##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780404_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 60690 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.87864557587741 34.0 34.0 34.0 31.0 34.0 2 33.271675729115174 34.0 34.0 34.0 32.0 34.0 3 33.4850387213709 34.0 34.0 34.0 34.0 34.0 4 33.5256549678695 34.0 34.0 34.0 34.0 34.0 5 33.54697643763388 34.0 34.0 34.0 34.0 34.0 6 37.27525127698138 38.0 38.0 38.0 36.0 38.0 7 36.916427747569614 38.0 37.0 38.0 35.0 38.0 8 36.92306805074971 38.0 37.0 38.0 35.0 38.0 9 36.71038062283737 38.0 37.0 38.0 34.0 38.0 10-11 36.45948261657604 38.0 37.0 38.0 34.0 38.0 12-13 36.319698467622345 38.0 37.0 38.0 34.0 38.0 14-15 36.48729609490855 38.0 37.0 38.0 34.0 38.0 16-17 36.575926841324765 38.0 37.0 38.0 34.0 38.0 18-19 36.634214862415554 38.0 37.0 38.0 34.0 38.0 20-21 36.40767012687428 38.0 37.0 38.0 34.0 38.0 22-23 36.423644752018454 38.0 37.0 38.0 34.0 38.0 24-25 35.63593672763223 38.0 36.0 38.0 31.0 38.0 26-27 35.86136101499423 38.0 36.5 38.0 31.5 38.0 28-29 35.76157521832262 38.0 36.5 38.0 31.5 38.0 30-31 35.39497446037238 38.0 36.0 38.0 29.0 38.0 32-33 35.853188334157196 38.0 37.0 38.0 32.0 38.0 34-35 35.712654473554124 38.0 36.5 38.0 31.0 38.0 36-37 35.72839841819081 38.0 37.0 38.0 31.5 38.0 38-39 35.048813643104296 38.0 35.5 38.0 29.0 38.0 40-41 35.13162794529576 37.0 35.5 38.0 29.0 38.0 42-43 35.77595155709342 38.0 36.5 38.0 32.5 38.0 44-45 36.01609820398748 38.0 37.0 38.0 33.0 38.0 46-47 36.24208271543912 38.0 37.0 38.0 34.0 38.0 48-49 35.991192947767345 38.0 37.0 38.0 32.5 38.0 50-51 36.07971659251936 38.0 37.0 38.0 33.5 38.0 52-53 35.99885483605207 38.0 37.0 38.0 33.0 38.0 54-55 36.21524139067392 38.0 37.0 38.0 34.0 38.0 56-57 36.19218981710331 38.0 37.0 38.0 34.0 38.0 58-59 36.194809688581316 38.0 37.0 38.0 34.0 38.0 60-61 35.92308452792882 38.0 37.0 38.0 32.5 38.0 62-63 36.287345526445876 38.0 37.0 38.0 34.0 38.0 64-65 36.259795682979075 38.0 37.0 38.0 34.0 38.0 66-67 35.36020761245675 38.0 36.0 38.0 31.0 38.0 68-69 34.02140385565991 37.5 34.0 38.0 23.0 38.0 70-71 33.089347503707366 37.0 32.5 38.0 16.0 38.0 72-73 32.127096721041354 37.0 29.0 38.0 11.0 38.0 74-75 32.43133135607184 37.0 31.5 38.0 11.0 38.0 76 32.0396605701104 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5233732812112919 1101 2 0.46773321707377846 1101 3 -0.3517176444368033 1101 4 0.3435824927972888 1101 5 0.4243283729157383 1101 6 -1.4405916081859615 1101 7 0.04402098596460746 1101 8 0.4097648152194182 1101 9 -0.04533351224515769 1101 10-11 -0.16563926805061158 1101 12-13 0.6750194563699949 1101 14-15 0.6237722392436424 1101 16-17 0.11724466319238047 1101 18-19 -0.16675578183888717 1101 20-21 -0.3073450006836893 1101 22-23 0.344581728079838 1101 24-25 -1.5864203989034849 1101 26-27 -0.5045354867057341 1101 28-29 0.6525025696080036 1101 30-31 0.5385442881936982 1101 32-33 0.4772849451788659 1101 34-35 0.19840722147090162 1101 36-37 0.24714718799329205 1101 38-39 0.4509725759586445 1101 40-41 0.7221804090696438 1101 42-43 0.30873427029190736 1101 44-45 0.38873310061516975 1101 46-47 0.7430071447161808 1101 48-49 -1.2919078286611239 1101 50-51 -1.1675168834076644 1101 52-53 -1.1898602411281232 1101 54-55 -0.22925508579724152 1101 56-57 -1.152804592301493 1101 58-59 -1.0315712830712798 1101 60-61 -1.726968446153748 1101 62-63 -0.7605751684947819 1101 64-65 -0.8817109843207049 1101 66-67 -1.5508067823076033 1101 68-69 -0.8652287314939002 1101 70-71 -0.9963139037150981 1101 72-73 -1.5837517631823772 1101 74-75 -0.9620541587111333 1101 76 -0.8104038629854955 1102 1 -0.3023240612003093 1102 2 0.2558289781104577 1102 3 0.501302342385074 1102 4 0.4353461651012651 1102 5 0.3186837430013938 1102 6 0.20368688950786407 1102 7 0.3762854269925029 1102 8 0.5145309035128491 1102 9 0.5107833337904211 1102 10-11 0.3215120607435935 1102 12-13 0.6986522259724595 1102 14-15 -0.26496046446819577 1102 16-17 0.11632219449042225 1102 18-19 -0.41116606077584805 1102 20-21 0.5483545380819592 1102 22-23 0.6897607309350988 1102 24-25 -5.049499687284772 1102 26-27 -0.8458708699695165 1102 28-29 0.2589378869811654 1102 30-31 1.6518541937504665 1102 32-33 0.39309869435523126 1102 34-35 0.39768242463365056 1102 36-37 -2.070533462127507 1102 38-39 -0.0938330467077364 1102 40-41 0.8144931698866955 1102 42-43 0.8966762865449311 1102 44-45 0.6315180775534586 1102 46-47 1.1771384866885057 1102 48-49 -3.222239478123022 1102 50-51 -0.6301569009784984 1102 52-53 -0.6886083193183268 1102 54-55 0.12822349975075298 1102 56-57 0.8111093106196563 1102 58-59 -0.4388631785248194 1102 60-61 -5.769314152281574 1102 62-63 -2.5644187880862575 1102 64-65 -0.18485177061545244 1102 66-67 0.861251201439373 1102 68-69 -1.3951430736858654 1102 70-71 -1.730071865498541 1102 72-73 -5.3392536300833235 1102 74-75 -3.0949775059547093 1102 76 -1.2693540057485073 1103 1 0.642980338147936 1103 2 0.561598493737776 1103 3 0.12839462500986798 1103 4 0.3271029418750757 1103 5 0.1099739222341043 1103 6 0.18639315524627165 1103 7 0.6217675777849792 1103 8 0.43743121457787737 1103 9 0.009598324162219285 1103 10-11 0.3842805394874773 1103 12-13 -0.6427231258272812 1103 14-15 -0.4573023397459295 1103 16-17 0.047036162791414426 1103 18-19 0.5173260144722533 1103 20-21 -0.09353184911993395 1103 22-23 0.5622240696997238 1103 24-25 -2.825273646698193 1103 26-27 -0.7135571386704456 1103 28-29 -0.4530307102636826 1103 30-31 0.7836124517863183 1103 32-33 1.2760419620192778 1103 34-35 0.04299423029207361 1103 36-37 -0.8180893797693329 1103 38-39 0.3124400980115638 1103 40-41 0.30203205301511815 1103 42-43 -0.24611737365356134 1103 44-45 1.0167202698212705 1103 46-47 0.9994425897843442 1103 48-49 -2.0674290796635333 1103 50-51 -0.48977029158729835 1103 52-53 0.5532632552632251 1103 54-55 -0.3642590954203371 1103 56-57 0.6502627693665062 1103 58-59 -0.4785239695187471 1103 60-61 -4.918588333884301 1103 62-63 -1.345358648847629 1103 64-65 -0.2383068143126863 1103 66-67 -0.07735048719933957 1103 68-69 0.10385707444034153 1103 70-71 0.2321744683779201 1103 72-73 -4.546722893896089 1103 74-75 -1.5780726029773753 1103 76 -3.33438140909616 1104 1 -0.47102074984336184 1104 2 0.2976586052568848 1104 3 0.3539488205763064 1104 4 -0.14736326239529518 1104 5 -0.061656704720881805 1104 6 0.3410579179281754 1104 7 0.24494859233686128 1104 8 0.2585246458304269 1104 9 0.8504115976560342 1104 10-11 -0.4704386227592252 1104 12-13 0.05439029726533562 1104 14-15 0.5312737516453367 1104 16-17 0.35225172106694913 1104 18-19 0.6823266944668163 1104 20-21 0.3792853683423161 1104 22-23 0.4659368399975605 1104 24-25 0.913438443605088 1104 26-27 0.9134318454176764 1104 28-29 1.2394757496846367 1104 30-31 0.5158985934971838 1104 32-33 0.1660528419322418 1104 34-35 0.7112928839028427 1104 36-37 1.2282522494976291 1104 38-39 1.5168124630326503 1104 40-41 1.6675121291745043 1104 42-43 0.6055078133449427 1104 44-45 0.932128946551849 1104 46-47 0.8951144244096696 1104 48-49 0.17806595637618727 1104 50-51 0.8585569217949924 1104 52-53 0.5246184844213886 1104 54-55 0.3717330166427715 1104 56-57 0.17258359079993113 1104 58-59 -0.17024843572301762 1104 60-61 1.1312892670948926 1104 62-63 0.37963455120677736 1104 64-65 0.8115537868025058 1104 66-67 -0.24816512068227325 1104 68-69 1.4232165195647752 1104 70-71 1.5381890202615125 1104 72-73 1.8373210337524881 1104 74-75 0.9918478376591011 1104 76 0.7873376171516284 1105 1 0.620120930114112 1105 2 0.4112285148794257 1105 3 0.23476354678577138 1105 4 -0.1869034006196415 1105 5 -0.25141614119084466 1105 6 0.16485510027798966 1105 7 0.6259959075947066 1105 8 -0.2877273477899891 1105 9 0.48621037490217844 1105 10-11 0.37932546549170354 1105 12-13 -0.3121866564826732 1105 14-15 0.0018678505289102532 1105 16-17 0.34949387949332333 1105 18-19 0.42152791722067917 1105 20-21 0.13142851028809588 1105 22-23 0.2744994396785856 1105 24-25 0.5254134568958904 1105 26-27 0.4917811277143329 1105 28-29 1.0857510022098893 1105 30-31 1.0342731283190858 1105 32-33 0.6211133150848127 1105 34-35 -0.8781209916952406 1105 36-37 0.4080601973976812 1105 38-39 -0.07841613877490516 1105 40-41 0.06346175703203016 1105 42-43 0.3589301950568 1105 44-45 0.7630865488910317 1105 46-47 0.8168315961268391 1105 48-49 -0.4533302424542285 1105 50-51 0.3383216745860622 1105 52-53 0.48845088739852116 1105 54-55 0.5112033781526009 1105 56-57 0.4953341224320411 1105 58-59 -0.139662975861242 1105 60-61 0.7597282623102046 1105 62-63 -1.0209004036045002 1105 64-65 0.2980303949896381 1105 66-67 0.3677472929275041 1105 68-69 0.7632783217976282 1105 70-71 1.588345187193994 1105 72-73 1.3897200236514777 1105 74-75 0.3831456845490564 1105 76 0.7834832843499271 1106 1 -1.648997137353014 1106 2 0.09783514724077236 1106 3 0.23787246616826963 1106 4 0.10233330606989455 1106 5 -0.025465598023316716 1106 6 -0.0348661690187555 1106 7 -0.29438716844875046 1106 8 0.28202679743585435 1106 9 -0.5933708143488232 1106 10-11 0.3337101138622032 1106 12-13 1.1100010221908576 1106 14-15 0.002475340523190539 1106 16-17 0.2576914209455765 1106 18-19 0.5223355215660135 1106 20-21 0.30000698370147205 1106 22-23 0.6625604745556544 1106 24-25 -0.6670536671750114 1106 26-27 1.0713265822597862 1106 28-29 0.4585533178188044 1106 30-31 0.7607954267753456 1106 32-33 -0.142233597436622 1106 34-35 1.1161257207118496 1106 36-37 1.2103222042587447 1106 38-39 0.12577196871508534 1106 40-41 1.1665742498850946 1106 42-43 0.9844662156399906 1106 44-45 1.2522768004633562 1106 46-47 1.0502506927534796 1106 48-49 1.308265669495455 1106 50-51 0.2890435156380917 1106 52-53 0.5993178113550712 1106 54-55 0.4760582952480874 1106 56-57 0.6573016753194167 1106 58-59 0.9793157262542493 1106 60-61 1.1120903263467952 1106 62-63 1.1839487959380932 1106 64-65 -0.6638406741927554 1106 66-67 0.4401279390109849 1106 68-69 1.848737870110952 1106 70-71 0.7024282346496804 1106 72-73 0.8509478681340426 1106 74-75 0.24540626202189486 1106 76 1.0529229641669673 1107 1 0.7218658382114782 1107 2 -0.5396532536252963 1107 3 -0.03700123215114104 1107 4 -0.1894493519360836 1107 5 0.08722643208287906 1107 6 0.3288826710678521 1107 7 0.6042621768445784 1107 8 -0.1826602837065323 1107 9 -0.436138109946306 1107 10-11 0.5877053711052227 1107 12-13 -0.01851738176831219 1107 14-15 0.1164046115820625 1107 16-17 0.4453396321013656 1107 18-19 0.5207889703751718 1107 20-21 0.5820463692051305 1107 22-23 0.03553707008097007 1107 24-25 0.6145217529713989 1107 26-27 -0.4095477346801033 1107 28-29 0.56279086808172 1107 30-31 0.5490210170423921 1107 32-33 1.1251651034603753 1107 34-35 -0.39727772294213537 1107 36-37 0.7301696112079128 1107 38-39 0.534164586510741 1107 40-41 0.5289827456062994 1107 42-43 1.0221505129902368 1107 44-45 0.5665360024429447 1107 46-47 0.9789031311491954 1107 48-49 0.8490946209337551 1107 50-51 1.1320685697673838 1107 52-53 0.8886775391620674 1107 54-55 0.7537673374173792 1107 56-57 0.9153695173649794 1107 58-59 0.7899663087885287 1107 60-61 1.2244730462148183 1107 62-63 0.5906402074238173 1107 64-65 0.9705030725852666 1107 66-67 -0.051108270907562314 1107 68-69 0.8841023406749287 1107 70-71 -0.22199696382438816 1107 72-73 0.5905086475958399 1107 74-75 -1.6464898097380782 1107 76 0.1030312227503245 1108 1 -0.280912711385632 1108 2 0.2546414578686367 1108 3 0.06710175942058783 1108 4 0.3421798043110229 1108 5 0.2441218659997091 1108 6 -0.1482869130758928 1108 7 -0.13992602358146655 1108 8 -0.4045737781957399 1108 9 0.08928561886725106 1108 10-11 0.6344776226565756 1108 12-13 1.0576611863524619 1108 14-15 0.5752820775448271 1108 16-17 -0.46968305938700894 1108 18-19 0.21463911393356483 1108 20-21 0.8372098405397637 1108 22-23 -0.04610597891412027 1108 24-25 1.075297894492195 1108 26-27 -0.3269785024859715 1108 28-29 -0.5411268868502077 1108 30-31 0.20392515708128656 1108 32-33 0.6802266946431104 1108 34-35 0.36279451934738915 1108 36-37 0.8196389082348645 1108 38-39 0.6359521200848803 1108 40-41 1.220193267047442 1108 42-43 0.1739982484723086 1108 44-45 0.885791351150921 1108 46-47 0.7237396598145622 1108 48-49 0.8201513959638476 1108 50-51 0.7005135081762077 1108 52-53 0.26225353249353844 1108 54-55 -0.3516786197929562 1108 56-57 0.29950399916548065 1108 58-59 0.6142693559312562 1108 60-61 1.0297430286173395 1108 62-63 0.6101826729234929 1108 64-65 0.6705586435772091 1108 66-67 0.7110478835797949 1108 68-69 0.9651433705906456 1108 70-71 -1.0737150336586012 1108 72-73 -1.1589124879047645 1108 74-75 -0.9949199292157154 1108 76 -0.7528546714795539 1109 1 -1.2269954650514165 1109 2 0.08260266096455382 1109 3 0.18868498661760924 1109 4 0.0215175137261312 1109 5 -0.15390563146934966 1109 6 -0.2959633442832299 1109 7 -0.39883330943104767 1109 8 0.2905700773282476 1109 9 0.6235921405353864 1109 10-11 0.3389451449451997 1109 12-13 0.20868499354360637 1109 14-15 0.8198854543124057 1109 16-17 0.7106670271549049 1109 18-19 0.7501866140152131 1109 20-21 1.0518337914231424 1109 22-23 0.4926582678446394 1109 24-25 -0.3444630538765807 1109 26-27 0.24762716029447063 1109 28-29 -0.9352191764553837 1109 30-31 0.10314464697384551 1109 32-33 0.272042768579297 1109 34-35 -0.3834743810804184 1109 36-37 1.121653865290483 1109 38-39 1.1797945811410244 1109 40-41 0.34540145750496976 1109 42-43 1.1317559964230597 1109 44-45 0.6234550460008634 1109 46-47 0.8308883486228993 1109 48-49 0.4615902777769847 1109 50-51 0.03876176917355423 1109 52-53 -0.3230474047717351 1109 54-55 -0.20156921774860592 1109 56-57 0.28715952968375547 1109 58-59 0.13294934678720693 1109 60-61 1.0899141530090972 1109 62-63 0.4553706158384898 1109 64-65 0.8337962909433969 1109 66-67 0.0018940349138460988 1109 68-69 -0.405451972474566 1109 70-71 0.12622944888605758 1109 72-73 -0.5361535969475781 1109 74-75 -0.49111535065851086 1109 76 -0.13099385700582644 1110 1 -0.3628336153404348 1110 2 -0.409331738820363 1110 3 0.12839462500986798 1110 4 -0.10894356975283159 1110 5 0.2785785733968922 1110 6 -0.051978937776979706 1110 7 0.819441996389628 1110 8 0.5653381913220628 1110 9 0.6782029753250072 1110 10-11 -0.7145566698148471 1110 12-13 0.6770443160331823 1110 14-15 0.9816511486261632 1110 16-17 0.5470361627914144 1110 18-19 0.15686089819318738 1110 20-21 0.8396076857637809 1110 22-23 -0.4116131396026006 1110 24-25 -0.7409713211167954 1110 26-27 1.0451637915621177 1110 28-29 0.29406231299213204 1110 30-31 -0.291968943562523 1110 32-33 0.7760419620192778 1110 34-35 0.8569477186641663 1110 36-37 1.0365617830213623 1110 38-39 0.585695911965054 1110 40-41 -0.5351772493104647 1110 42-43 0.21027797518365077 1110 44-45 0.917883060518939 1110 46-47 0.7000239851331855 1110 48-49 1.3104778970806592 1110 50-51 -0.05953773344776181 1110 52-53 0.7422167436353106 1110 54-55 0.019461834812226186 1110 56-57 1.0252627693665062 1110 58-59 -0.42910536486758843 1110 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44-45 -0.27483011777563604 2116 46-47 -0.6804411311458836 2116 48-49 -0.0604135757875639 2116 50-51 -0.6170571132927236 2116 52-53 -0.0851475974499607 2116 54-55 -0.06891025821103369 2116 56-57 -0.4899310290831096 2116 58-59 -1.766973581921853 2116 60-61 -0.9343247679928268 2116 62-63 0.5441374751833763 2116 64-65 -1.1578029383436927 2116 66-67 -0.11138149495128147 2116 68-69 -0.4732747085054001 2116 70-71 1.6470194296182328 2116 72-73 1.3550988115302616 2116 74-75 1.8121987148520802 2116 76 0.4183317691984172 2117 1 0.6669677091628756 2117 2 0.3906836623809298 2117 3 0.22750135533483729 2117 4 0.14956559396193825 2117 5 0.22040053550993832 2117 6 0.4407054916085116 2117 7 0.4485425662340816 2117 8 0.13641473529249026 2117 9 -1.040994622087588 2117 10-11 -0.8362261600350891 2117 12-13 -1.7696482759889918 2117 14-15 0.03592113168483735 2117 16-17 -0.83356602417917 2117 18-19 -1.270637946716711 2117 20-21 -0.4717661013916157 2117 22-23 0.18959200439863366 2117 24-25 -2.032458307101706 2117 26-27 -1.3558257356210106 2117 28-29 -1.1398472819562784 2117 30-31 -0.4704303635532767 2117 32-33 -2.3793946620373987 2117 34-35 0.6347853900025342 2117 36-37 -1.0935013619039466 2117 38-39 0.4839709774201566 2117 40-41 0.32480657942817714 2117 42-43 0.5994855833770885 2117 44-45 -0.33604424581096026 2117 46-47 -0.9683881510134427 2117 48-49 -1.0901266000701213 2117 50-51 -0.5802138461845558 2117 52-53 0.5011879433888922 2117 54-55 -0.2718903906613619 2117 56-57 0.5055877347137994 2117 58-59 -1.0909912419660017 2117 60-61 -0.5635521410612583 2117 62-63 -1.0278051296731334 2117 64-65 -0.963145101699105 2117 66-67 -0.619703790757022 2117 68-69 0.20200122968444845 2117 70-71 -0.5996251619932451 2117 72-73 0.47381845832937586 2117 74-75 0.7403664852715082 2117 76 1.037095566107908 2118 1 -0.4846284871353035 2118 2 0.355575834226741 2118 3 -0.4990651245429021 2118 4 0.19800335928712087 2118 5 0.3241957469210419 2118 6 0.3348725827954695 2118 7 0.6731987047974997 2118 8 0.5048134447508019 2118 9 -1.4297278535360505 2118 10-11 -0.7472042547880093 2118 12-13 -1.022195397741406 2118 14-15 0.5955450067060681 2118 16-17 -3.2153371228376812 2118 18-19 -0.9349697517769968 2118 20-21 -0.06386577875619537 2118 22-23 0.5946480410771855 2118 24-25 0.697907629390059 2118 26-27 -2.181878545945338 2118 28-29 -0.701763566554682 2118 30-31 -0.9084716269256674 2118 32-33 -0.1337672210457157 2118 34-35 -0.35146015253440055 2118 36-37 -2.633533625446134 2118 38-39 -0.48138816674693174 2118 40-41 -0.7399327651124921 2118 42-43 0.6484115470381511 2118 44-45 -2.489317297262815 2118 46-47 -0.14762061832537654 2118 48-49 -0.002208447582432882 2118 50-51 -1.079364805600413 2118 52-53 -1.0420706743730364 2118 54-55 -0.9817307710315433 2118 56-57 0.4519920478399726 2118 58-59 -0.5814607614090264 2118 60-61 -0.8739401526082133 2118 62-63 -1.5980420119961138 2118 64-65 -0.37023883577958827 2118 66-67 -0.291125084694869 2118 68-69 -1.4393003495310381 2118 70-71 -0.3879805703817709 2118 72-73 1.2368936833251354 2118 74-75 0.40142948408285406 2118 76 0.2555112563779005 2119 1 0.6966069441001252 2119 2 -0.8851001564492478 2119 3 -0.09871547419325566 2119 4 -0.42029500901124806 2119 5 -0.09363642091112467 2119 6 -0.7018407039178172 2119 7 -0.9701579385591401 2119 8 0.11437055430791787 2119 9 0.16759149378329852 2119 10-11 -0.385316142899903 2119 12-13 -0.0659016914476993 2119 14-15 -0.34996781380675657 2119 16-17 -0.24272640022695668 2119 18-19 -0.32366439047163453 2119 20-21 -0.5816396715300911 2119 22-23 0.00286482429397239 2119 24-25 1.3514624079448367 2119 26-27 0.48391399484719955 2119 28-29 0.5129800231889092 2119 30-31 1.4542906108365727 2119 32-33 0.40935632207782646 2119 34-35 0.536651735577486 2119 36-37 -0.03621427812679201 2119 38-39 -1.334535019893785 2119 40-41 -1.40485118003091 2119 42-43 0.13646516009175969 2119 44-45 0.3769414439959249 2119 46-47 -0.041560012264767465 2119 48-49 -0.10652080189478852 2119 50-51 1.0791433529077423 2119 52-53 0.052917670615308054 2119 54-55 0.4104603711595942 2119 56-57 -1.362401891553965 2119 58-59 -0.04678710173536871 2119 60-61 0.8355586818906247 2119 62-63 0.3031817642276735 2119 64-65 0.21804787750711796 2119 66-67 0.5795042859344974 2119 68-69 1.1717136364829486 2119 70-71 0.9526371452359541 2119 72-73 1.760786457217911 2119 74-75 1.2802173628707365 2119 76 1.0404762913429337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 2.0 21 0.0 22 7.0 23 10.0 24 40.0 25 81.0 26 173.0 27 272.0 28 533.0 29 859.0 30 1335.0 31 2014.0 32 3052.0 33 4504.0 34 6619.0 35 8558.0 36 11683.0 37 20946.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.700609655626955 22.423793046630415 11.248970176305818 33.62662712143681 2 16.651837205470425 17.599275004119296 44.48179271708683 21.267095073323446 3 15.552809359037735 26.71280276816609 36.28769154720712 21.44669632558906 4 11.268742791234141 23.199868182567144 35.155709342560556 30.37567968363816 5 10.471247322458394 39.28159499093755 37.225243038391824 13.021914648212226 6 21.942659416707862 38.77574559235459 16.96819904432361 22.31339594661394 7 21.791069368924042 36.1624649859944 19.136595814796507 22.909869830285054 8 19.299719887955185 35.846103147141214 23.252595155709344 21.601581809194265 9 18.80210907892569 20.677212061295105 18.146317350469598 42.37436150930961 10-11 19.60289998352282 25.24056681496128 30.54209919261822 24.614434008897675 12-13 22.918108419838525 19.475201845444058 27.82995551161641 29.776734223101002 14-15 23.124897017630584 23.166913824353273 20.695336958312737 33.01285219970341 16-17 24.25358378645576 27.63140550337782 20.837040698632396 27.277970011534023 18-19 22.517712967539957 26.629593013676057 21.41456582633053 29.43812819245345 20-21 20.839512275498436 24.440599769319494 21.36101499423299 33.358872960949085 22-23 23.30779370571758 29.61113857307629 21.49942329873126 25.58164442247487 24-25 23.84247816773768 31.819904432361177 21.747404844290656 22.590212555610478 26-27 25.168067226890756 29.158016147635525 25.22738507167573 20.44653155379799 28-29 23.607678365463833 26.122919756137748 28.102652825836216 22.166749052562203 30-31 27.10743120777723 23.81693854012193 27.043170209260175 22.032460042840665 32-33 22.288680177953534 26.251441753171857 30.83209754490031 20.627780523974295 34-35 19.998352282089307 25.497610809029492 32.034107760751354 22.469929148129843 36-37 22.233481627945295 27.330696984676223 28.353929807217003 22.081891580161475 38-39 20.871642774756964 23.859779205799967 28.524468610973802 26.744109408469267 40-41 19.380458065579173 24.583127368594496 31.030647553138902 25.00576701268743 42-43 24.668808699950567 23.011204481792717 32.200527269731424 20.119459548525292 44-45 19.08634041852035 25.531389026198713 29.92997198879552 25.452298566485414 46-47 24.522985664854176 20.757950238919097 27.977426264623496 26.74163783160323 48-49 22.05717581150107 20.589059153072995 29.1646070192783 28.189158016147637 50-51 17.217828307793706 20.94496622178283 33.6686439281595 28.168561542263966 52-53 18.386884165430878 20.916131158345692 38.01861921239084 22.67836546383259 54-55 17.1280276816609 21.25308947108255 34.13165266106443 27.487230186192125 56-57 22.696490360850223 21.212720382270554 30.219146482122262 25.87164277475696 58-59 17.724501565332016 27.54325259515571 33.17185697808535 21.560388861426922 60-61 19.490031306640304 35.333662876915476 24.667160982039874 20.50914483440435 62-63 15.259515570934257 41.357719558411596 22.67095073323447 20.711814137419672 64-65 15.393804580655793 42.74097874443895 21.27615752183226 20.589059153072995 66-67 15.232328225407809 41.51342890097215 21.17152743450321 22.082715439116825 68-69 15.194430713461857 39.90360850222442 21.251441753171857 23.65051903114187 70-71 15.904597132970835 37.2194760257044 21.744933267424617 25.130993573900145 72-73 16.249794035261164 34.37304333498105 22.488877904102818 26.88828472565497 74-75 17.09919261822376 34.008073817762394 22.67836546383259 26.21436810018125 76 17.001153402537486 34.285714285714285 22.315043664524634 26.398088647223595 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 1.5 12 3.0 13 2.0 14 0.5 15 1.0 16 2.5 17 3.0 18 5.0 19 6.5 20 14.5 21 23.0 22 32.0 23 53.5 24 76.5 25 87.0 26 108.5 27 161.0 28 234.5 29 277.0 30 321.0 31 419.0 32 620.0 33 767.0 34 883.5 35 1068.5 36 1264.5 37 1392.0 38 1687.0 39 2330.5 40 3744.5 41 5624.5 42 6439.0 43 6465.0 44 6033.0 45 5100.5 46 4626.0 47 4381.5 48 3856.0 49 3302.0 50 3029.0 51 2794.5 52 2337.0 53 1904.0 54 1694.0 55 1608.5 56 1312.0 57 966.5 58 832.0 59 690.0 60 450.0 61 308.5 62 265.0 63 204.5 64 123.5 65 86.5 66 57.5 67 45.0 68 28.0 69 7.0 70 3.5 71 4.0 72 2.0 73 0.5 74 0.5 75 0.0 76 0.5 77 1.0 78 1.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 60690.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.14598780688746 #Duplication Level Percentage of deduplicated Percentage of total 1 65.88231093337143 30.402043170209257 2 17.132043133614225 15.811501071016643 3 7.430550596300793 10.286702916460703 4 3.4849675069627937 6.432690723348163 5 1.9245875883739199 4.440599769319493 6 1.1354709705063202 3.1438457736035588 7 0.8248232521602514 2.6643598615916955 8 0.5427408412483039 2.003624979403526 9 0.4177676212240234 1.7350469599604548 >10 1.1533242876526457 7.515241390673917 >50 0.021423980575590945 0.7381776239907728 >100 0.03570663429265158 2.5605536332179932 >500 0.0035706634292651572 0.84857472400725 >1k 0.010711990287795473 11.417037403196574 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT 3010 4.959630911188005 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT 2877 4.740484429065743 TruSeq Adapter, Index 9 (95% over 23bp) CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 1042 1.7169220629428241 RNA PCR Primer, Index 9 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 515 0.84857472400725 TruSeq Adapter, Index 9 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 344 0.5668149612786291 No Hit TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT 221 0.3641456582633053 RNA PCR Primer, Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT 189 0.31141868512110726 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 147 0.2422145328719723 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 121 0.1993738671939364 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 115 0.18948755972977427 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT 105 0.17301038062283738 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT 105 0.17301038062283738 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT 104 0.17136266271214368 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 103 0.16971494480145 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 90 0.14829461196243204 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT 89 0.14664689405173834 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGTCTT 76 0.12522656121272038 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 73 0.12028340748063931 TruSeq Adapter, Index 9 (95% over 21bp) CTTATTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 61 0.10051079255231504 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.004943153732081068 0.0 5 0.0 0.0 0.0 0.013181743285549514 0.0 6 0.0 0.0 0.0 0.013181743285549514 0.0 7 0.0 0.0 0.0 0.013181743285549514 0.0 8 0.0 0.0 0.0 0.013181743285549514 0.0 9 0.0 0.0 0.0 0.019772614928324272 0.0 10 0.0 0.0 0.0 0.02306805074971165 0.0 11 0.0 0.0 0.0 0.024715768660405337 0.0 12 0.0 0.0 0.0 0.026363486571099028 0.0 13 0.0 0.0 0.0 0.026363486571099028 0.0 14 0.0 0.0 0.0 0.031306640303180096 0.0 15 0.0 0.0 0.0 0.03460207612456748 0.0 16 0.0 0.0 0.0 0.03789751194595485 0.0 17 0.0 0.0 0.0 0.03789751194595485 0.0 18 0.0 0.0 0.0 0.0461361014994233 0.0 19 0.0 0.0 0.0 0.0461361014994233 0.0 20 0.0 0.0 0.0 0.052726973142198055 0.0 21 0.0 0.0 0.0 0.05767012687427912 0.0 22 0.0 0.0 0.0 0.060965562695666504 0.0 23 0.0 0.0 0.0 0.06920415224913495 0.0 24 0.0 0.0 0.0 0.07085187015982863 0.0 25 0.0 0.0 0.0 0.0757950238919097 0.0 26 0.0 0.0 0.0 0.08568133135607184 0.0 27 0.0 0.0 0.0 0.10051079255231504 0.0 28 0.0 0.0 0.0 0.13181743285549513 0.0 29 0.0 0.0 0.0 0.15818091942659418 0.0 30 0.0 0.0 0.0 0.19278299555116163 0.0 31 0.0 0.0 0.0 0.23562366122919756 0.0 32 0.0 0.0 0.0 0.29988465974625145 0.0 33 0.0 0.0 0.0 0.3954522985664854 0.0 34 0.0 0.0 0.0 0.4778381941011699 0.0 35 0.0 0.0 0.0 0.5767012687427913 0.0 36 0.0 0.0 0.0 0.7859614434008898 0.0 37 0.0 0.0 0.0 1.0759597956829792 0.0 38 0.0 0.0 0.0 1.38902619871478 0.0 39 0.0 0.0 0.0 1.824023727137914 0.0 40 0.0 0.0 0.0 2.26890756302521 0.0 41 0.0 0.0 0.0 3.0070851870159827 0.0 42 0.0 0.0 0.0 3.87213709013017 0.0 43 0.0 0.0 0.0 4.720711814137419 0.0 44 0.0 0.0 0.0 5.4967869500741475 0.0 45 0.0 0.0 0.0 6.312407315867524 0.0 46 0.0 0.0 0.0 7.015982863733729 0.0 47 0.0 0.0 0.0 7.7656945130993575 0.0 48 0.0 0.0 0.0 8.456088317680013 0.0 49 0.0 0.0 0.0 9.09375514911847 0.0 50 0.0 0.0 0.0 9.672104135771956 0.0 51 0.0 0.0 0.0 10.280112044817928 0.0 52 0.0 0.0 0.0 10.935903773274015 0.0 53 0.0 0.0 0.0 11.711978909210742 0.0 54 0.0 0.0 0.0 12.486406327236777 0.0 55 0.0 0.0 0.0 13.217993079584774 0.0 56 0.0 0.0 0.0 13.85895534684462 0.0 57 0.0 0.0 0.0 14.4389520514088 0.0 58 0.0 0.0 0.0 15.018948755972977 0.0 59 0.0 0.0 0.0 15.585763717251606 0.0 60 0.0 0.0 0.0 16.045477014335145 0.0 61 0.0 0.0 0.0 16.557917284560883 0.0 62 0.0 0.0 0.0 17.185697808535178 0.0 63 0.0 0.0 0.0 17.75745592354589 0.0 64 0.0 0.0 0.0 18.330861756467293 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCCTC 15 0.002214729 70.00001 47 CTATACA 80 0.0 61.25 1 CTTTACA 160 0.0 54.6875 1 TTTACAC 165 0.0 53.030308 2 GGTCGGA 20 0.006920488 52.5 1 GGAGAGC 20 0.006920488 52.5 6 CGGTTTC 20 0.006920488 52.5 55 GTCCTCT 20 0.006920488 52.5 48 GCGCCTA 20 0.006920488 52.5 18 CCGTCCT 20 0.006920488 52.5 46 GGGGTTC 20 0.006920488 52.5 19 AGCGCCT 20 0.006920488 52.5 17 GTGTAAT 20 0.006920488 52.5 1 GCCGTCC 20 0.006920488 52.5 45 CCTCTGC 20 0.006920488 52.5 50 TTACACA 185 0.0 47.2973 3 TCGTTAC 30 5.8352214E-4 46.666668 13 ATATCGT 40 4.5432782E-5 43.75 37 GTCTTAG 35 0.001246389 40.0 1 GGCTGTG 35 0.001246389 40.0 7 >>END_MODULE