FastQCFastQC Report
Wed 25 May 2016
SRR1780403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874957
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC353.22896E-431.4089229
CGGTGGT2900.025.0073943
GTCATAG450.0013969424.4473381
GTACTAG551.5918467E-424.0028421
TCGGTGG4500.022.46840542
CTATACA2450.022.4516351
CTTATAA803.6099354E-622.0026051
TCACGTA856.1160845E-620.70359425
CGTGTAT755.4509917E-520.54281835
CGGTTCA654.8643094E-420.34852821
CGGGTAG1101.258486E-720.00923735
GGTATGA1452.9831426E-1019.70617141
CGACAAT1352.5393092E-919.58926217
GCGGGTA808.953126E-519.25889234
TCTCGGG2550.018.9598543
ATTCGGT708.018081E-418.89289718
CGTTGAA708.1707776E-418.83996642
CATCGTT708.1707776E-418.83996632
GTCCAGG1102.7914684E-618.002131
TAGATCT8100.017.66247639
Produced by FastQC (version 0.11.5)