FastQCFastQC Report
Wed 25 May 2016
SRR1780403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874957
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT581406.644897977843482No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT461035.269173227941487No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC147871.6900259098447123No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC79790.9119305291574329No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG71750.8200402991232711No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT38380.43865012794914493No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC34110.38984772965985753No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC23170.2648130136681003No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG21610.24698356604953156No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG16120.1842376253918764No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC13840.15817920194935295No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC11660.1332636918157121No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC11460.13097786519794685No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG9300.10629093772608253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT51950.042.38971344
GCCGTCT56250.039.07103743
CTTTACA16900.036.9969861
CTATACA10800.036.896871
CTCTTGA250.002351404435.2031562
TAGACGC250.002352063835.20114521
TTTACAC18050.034.252382
TTACACA19450.031.9000323
GATTCGG502.3609227E-630.7992444
CTTTATA3150.030.7329142
GCGGGTA300.005746455429.33093335
TTATACA89000.029.1217122
CTTATAC90150.028.7927271
CTTATAA1700.028.491051
TGATTCG554.9599457E-627.9993143
TGCCGTC82900.026.51080342
TATACAC101100.025.8104233
TCGTTCA609.750827E-625.66603342
CGTTCAT609.750827E-625.66603343
ATTACAC2300.024.8717964
Produced by FastQC (version 0.11.5)