FastQCFastQC Report
Wed 25 May 2016
SRR1780402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47426
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCA150.002211996469.9978948
TCGACGC252.343365E-456.1167031
TTAGCTT200.006912017752.49841710
CACTGAG200.006912017752.4984177
TCTGTTC200.006912017752.4984178
ATTGACC200.006912017752.49841746
TGGCTGG200.006912017752.49841733
CGACGCC400.002396541434.9989432
ATAACCA400.002396541434.99894346
TTTCCTA450.004267944531.11017640
TCGACCC500.007069558428.0583521
GAGGCAA959.154412E-625.78869670
CGAGGCA1205.5723165E-520.41605269
ACGAGGC1806.9244E-515.55508870
GACGAGG2151.8981329E-514.650722569
CGACGAG2252.9151017E-513.99957868
CCGACGA2500.001115213411.19966367
GCCGACG3054.977379E-410.32755866
TGCCGAC3000.00507051039.33305365
GCTGCCG4056.78484E-48.64171470