##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780402_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47426 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.528359971323745 33.0 23.0 34.0 12.0 34.0 2 28.914414034495845 34.0 23.0 34.0 12.0 34.0 3 29.006072618394974 34.0 27.0 34.0 11.0 34.0 4 29.57196474507654 34.0 31.0 34.0 11.0 34.0 5 29.40039218993801 34.0 27.0 34.0 11.0 34.0 6 31.636022434951293 37.0 27.0 38.0 11.0 38.0 7 30.841901066925317 36.0 26.0 38.0 11.0 38.0 8 29.878020495087082 34.0 24.0 38.0 11.0 38.0 9 30.772635263357653 36.0 26.0 38.0 12.0 38.0 10-11 31.15104583983469 36.5 27.0 38.0 11.0 38.0 12-13 31.564679711550625 37.0 28.5 38.0 11.0 38.0 14-15 31.15515750853962 37.0 27.0 38.0 11.0 38.0 16-17 30.989952768523594 37.0 26.0 38.0 11.0 38.0 18-19 31.013484164804115 37.0 26.5 38.0 11.0 38.0 20-21 31.10970775523974 37.0 26.5 38.0 11.0 38.0 22-23 31.008697760721965 37.0 26.5 38.0 11.0 38.0 24-25 31.443596339560578 37.0 27.0 38.0 11.0 38.0 26-27 31.489657571796062 37.0 27.0 38.0 11.0 38.0 28-29 31.566819887825243 37.0 27.0 38.0 11.0 38.0 30-31 31.38351326276726 37.0 27.0 38.0 11.0 38.0 32-33 31.233194872011133 37.0 26.5 38.0 11.0 38.0 34-35 31.155663560072533 37.0 26.5 38.0 11.0 38.0 36-37 31.078596128705776 37.0 26.5 38.0 11.0 38.0 38-39 31.518354910808416 37.0 27.0 38.0 11.0 38.0 40-41 31.026356850672627 37.0 26.0 38.0 11.0 38.0 42-43 31.436785729346774 37.0 27.0 38.0 11.0 38.0 44-45 30.47978956690423 36.0 25.5 38.0 11.0 38.0 46-47 31.17023362712436 37.0 27.0 38.0 11.0 38.0 48-49 31.003162822080714 36.5 26.0 38.0 11.0 38.0 50-51 30.9327900307848 36.5 26.5 38.0 11.0 38.0 52-53 30.85516383418378 36.5 26.0 38.0 11.0 38.0 54-55 30.621578880782693 36.0 26.0 38.0 11.0 38.0 56-57 30.59840593767132 36.5 25.5 38.0 11.0 38.0 58-59 30.482836418841984 36.0 25.0 38.0 11.0 38.0 60-61 30.61725635727238 36.0 25.5 38.0 11.0 38.0 62-63 30.64049255682537 36.0 25.5 38.0 11.0 38.0 64-65 30.66893687007127 36.0 25.0 38.0 11.0 38.0 66-67 30.641335976046893 36.0 25.5 38.0 11.0 38.0 68-69 30.142970100788595 36.0 24.5 38.0 11.0 38.0 70-71 30.105321975287815 36.0 24.0 38.0 11.0 38.0 72-73 30.224180829081092 36.0 24.5 38.0 11.0 38.0 74-75 29.87865305950323 35.5 24.0 37.5 11.0 38.0 76 29.136549571964746 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.82506074022114 1101 2 -1.3582514193347706 1101 3 0.3784297883472263 1101 4 -2.7843762932103466 1101 5 -1.6609595822733212 1101 6 -2.556826190673519 1101 7 -2.834087534310566 1101 8 -1.799715503296735 1101 9 -0.8476418638224281 1101 10-11 -1.0737840820377826 1101 12-13 -1.681638320431908 1101 14-15 -0.4144494223778956 1101 16-17 -3.1201079697016603 1101 18-19 -1.4780422087469596 1101 20-21 -0.7027519892373739 1101 22-23 -0.9055949122093523 1101 24-25 -1.5816953778964375 1101 26-27 -1.7941965614937594 1101 28-29 -0.47974989052079664 1101 30-31 -2.220694232691365 1101 32-33 -0.7574578051711747 1101 34-35 -2.3009770641245915 1101 36-37 -1.3083179953562691 1101 38-39 -1.7169481266471287 1101 40-41 -0.5025655200130323 1101 42-43 -0.6172915907710106 1101 44-45 -1.1711558792915824 1101 46-47 0.2417312934018696 1101 48-49 -1.73483013669561 1101 50-51 -1.3222214900703406 1101 52-53 -0.5550478052879022 1101 54-55 -1.1957892979179583 1101 56-57 -0.3403954501325259 1101 58-59 -0.06383688550410582 1101 60-61 -0.3237997471437595 1101 62-63 -0.9536124520430533 1101 64-65 -0.6518329977641315 1101 66-67 -1.8168000283880836 1101 68-69 -0.7000306847758537 1101 70-71 0.45423559407759484 1101 72-73 -0.2333927822294406 1101 74-75 -1.0332338419769798 1101 76 -1.7966043570165802 1102 1 0.8585293624866068 1102 2 -0.22613190486231716 1102 3 0.6027528509055848 1102 4 0.05105788045912263 1102 5 1.0173784195940918 1102 6 0.8313624181033283 1102 7 1.1835361818425625 1102 8 1.8081276339581684 1102 9 1.393487921425006 1102 10-11 -0.6654040633636455 1102 12-13 -3.1278918218324705 1102 14-15 -0.7251403654217796 1102 16-17 -1.6418166531750522 1102 18-19 -1.1039759155630158 1102 20-21 0.2361485709867175 1102 22-23 -1.11958884311505 1102 24-25 0.26651890781784715 1102 26-27 -4.751187691279011 1102 28-29 0.3636207910851823 1102 30-31 0.6977231342554155 1102 32-33 -5.678267795834106 1102 34-35 -0.6763272041806125 1102 36-37 0.34352607560545323 1102 38-39 1.0548772701782703 1102 40-41 0.6419256097722155 1102 42-43 -0.16234294464589283 1102 44-45 -4.688662882092707 1102 46-47 -0.9986982117335188 1102 48-49 -0.17682360074789472 1102 50-51 -6.536273777652038 1102 52-53 -2.6774801115437405 1102 54-55 0.5339609355087482 1102 56-57 -1.300421127069967 1102 58-59 0.49732091094780984 1102 60-61 -0.16150983117737638 1102 62-63 -4.404009277439879 1102 64-65 -1.0899165645241666 1102 66-67 -0.26783877722095184 1102 68-69 -6.03762405545746 1102 70-71 0.04077854459113439 1102 72-73 -0.25836943955903635 1102 74-75 -6.060661479698737 1102 76 -3.123285029285487 1103 1 -1.3876948028552043 1103 2 -0.7587233198066734 1103 3 0.06300722292466077 1103 4 -0.14409315292720493 1103 5 -0.7950222163359548 1103 6 -0.5788124626597906 1103 7 0.5501895999665685 1103 8 2.0628971093158803 1103 9 -0.11962813580869991 1103 10-11 -0.3063023145560173 1103 12-13 -2.0506859394795285 1103 14-15 -1.8209273288558023 1103 16-17 -1.6748055243992148 1103 18-19 -0.8704703511750971 1103 20-21 -1.745866532351915 1103 22-23 -1.4098312914457338 1103 24-25 -0.7588189300199915 1103 26-27 -2.1660326833298846 1103 28-29 -0.851800512571419 1103 30-31 -1.283596420593554 1103 32-33 -5.535879083592452 1103 34-35 -2.5942524824000124 1103 36-37 -1.4128332248714983 1103 38-39 -0.27620381090281043 1103 40-41 -0.4787023711498861 1103 42-43 -0.7293143277937446 1103 44-45 -4.706548414684121 1103 46-47 -1.2240559223853467 1103 48-49 -1.0452655721310435 1103 50-51 -1.6225217903706408 1103 52-53 -1.6025596027997047 1103 54-55 -1.821861798990458 1103 56-57 -1.0433662781033526 1103 58-59 -0.25506382673104966 1103 60-61 -0.5815217548657685 1103 62-63 -0.24184711527771796 1103 64-65 -0.46720193813307276 1103 66-67 -1.223224309812366 1103 68-69 -3.4838680936132604 1103 70-71 -0.9289154140734155 1103 72-73 -0.17510234893900645 1103 74-75 -1.9603037690469058 1103 76 0.013241202828979226 1104 1 -1.4282353433957446 1104 2 -0.37238492661896316 1104 3 0.14566179530526213 1104 4 0.2994462769135957 1104 5 0.7123824486029555 1104 6 -0.3610227551440559 1104 7 0.8357319916733452 1104 8 1.6477730811781512 1104 9 1.3344629415700453 1104 10-11 0.9541567746701958 1104 12-13 -0.5232886792055567 1104 14-15 0.21905786181432063 1104 16-17 -0.7872638731589312 1104 18-19 0.5304922552669566 1104 20-21 0.9984874106321655 1104 22-23 0.4662794082951045 1104 24-25 0.7416901406945584 1104 26-27 0.5222309227419437 1104 28-29 -0.29894971656993263 1104 30-31 -0.0130947545439426 1104 32-33 0.5441838560595293 1104 34-35 0.36960349686692595 1104 36-37 0.23141890396967568 1104 38-39 -0.09751999009570156 1104 40-41 0.8385412423266061 1104 42-43 0.6480921883114021 1104 44-45 -3.4058264598525767 1104 46-47 0.21504070849210422 1104 48-49 0.8355656015083461 1104 50-51 1.333790686489781 1104 52-53 0.4565851583998537 1104 54-55 0.3897727807948108 1104 56-57 0.4806526037922403 1104 58-59 0.7247584613104188 1104 60-61 0.7251239319169045 1104 62-63 -0.16942023634399206 1104 64-65 0.1005044669021018 1104 66-67 0.15455243953972797 1104 68-69 -2.274177238374463 1104 70-71 0.08121972106172137 1104 72-73 0.9906233082250075 1104 74-75 -0.060359304033951844 1104 76 0.19752480149614016 1105 1 -0.8094853433957461 1105 2 -0.48017602250937585 1105 3 -0.3957765608591224 1105 4 -0.6787215313055839 1105 5 0.13318724312350483 1105 6 0.11628888869156029 1105 7 1.399345005371977 1105 8 0.31695116336993223 1105 9 -0.3120267844573483 1105 10-11 0.43643417193047185 1105 12-13 -0.5725609394795299 1105 14-15 -0.011721247774723054 1105 16-17 1.1877789350602477 1105 18-19 0.7436343785546278 1105 20-21 -0.9524543701897521 1105 22-23 -0.5034123725268174 1105 24-25 0.6165189078178486 1105 26-27 0.29697407342687043 1105 28-29 -1.1724086206795228 1105 30-31 -0.31459304221517215 1105 32-33 1.1476513218129547 1105 34-35 0.4161360311135063 1105 36-37 -0.05573863027690251 1105 38-39 0.15175227017827098 1105 40-41 0.21563884506633002 1105 42-43 0.8075099965305768 1105 44-45 1.1157488826131754 1105 46-47 0.4375150235606 1105 48-49 0.25405875219328067 1105 50-51 -0.4427076011814499 1105 52-53 -1.3513771703672681 1105 54-55 -1.1774361233147808 1105 56-57 -0.6342446564817301 1105 58-59 -0.23209085375807348 1105 60-61 -0.4947818900009082 1105 62-63 0.5741157225601192 1105 64-65 -0.2628945057006362 1105 66-67 0.5584479874849322 1105 68-69 0.7461994739543059 1105 70-71 0.9124697210617185 1105 72-73 0.2831746780880202 1105 74-75 -0.8999997149928518 1105 76 -0.5207114998737232 1106 1 -0.7994474646078658 1106 2 0.4628164017330505 1106 3 0.029223439140878327 1106 4 0.2654072565732051 1106 5 0.444740273426536 1106 6 0.23466010081277133 1106 7 1.5237768235537956 1106 8 2.1245269209456907 1106 9 1.655965639785073 1106 10-11 1.0476084143547126 1106 12-13 0.8091625453689559 1106 14-15 0.8775779946495206 1106 16-17 1.5308660562723695 1106 18-19 0.703956348251598 1106 20-21 0.9162956298102465 1106 22-23 0.5848452032307598 1106 24-25 0.9350795138784562 1106 26-27 0.5869361946389979 1106 28-29 -1.005363166134071 1106 30-31 0.02660013960300489 1106 32-33 1.5134657157523428 1106 34-35 1.2450186068710813 1106 36-37 1.3105492485109735 1106 38-39 1.1079075732085748 1106 40-41 0.42311990567239377 1106 42-43 0.8639493904699691 1106 44-45 1.0907488826131768 1106 46-47 0.816586993257566 1106 48-49 1.180573903708435 1106 50-51 0.7750007321518808 1106 52-53 0.09881222357212494 1106 54-55 0.8651775130488559 1106 56-57 1.0403765556394795 1106 58-59 0.2330606613934414 1106 60-61 2.0712218978778836 1106 62-63 1.0126573892267885 1106 64-65 1.1659880700569403 1106 66-67 2.0475578359697764 1106 68-69 1.9814267466815778 1106 70-71 1.0736439634859636 1106 72-73 1.086773162936506 1106 74-75 -0.7282194119625487 1106 76 -0.7576433180555391 1107 1 0.24290559620157026 1107 2 0.5016612258127715 1107 3 0.6332502847784625 1107 4 0.22249491164743773 1107 5 1.3087325451369267 1107 6 0.7977066739264593 1107 7 0.4270715154390885 1107 8 0.8300384116920796 1107 9 -1.5535116837862084 1107 10-11 0.22213048065530216 1107 12-13 0.5499852014600677 1107 14-15 -0.006624771264654328 1107 16-17 0.8735801095568902 1107 18-19 1.7099305194942254 1107 20-21 1.5387788512867537 1107 22-23 0.030060781835601347 1107 24-25 -0.15504149486671537 1107 26-27 0.43636165731949106 1107 28-29 -0.8424589562499953 1107 30-31 0.5385739040935533 1107 32-33 1.5003988050344326 1107 34-35 -0.17385977425562515 1107 36-37 0.2279023093204131 1107 38-39 0.9114208943393471 1107 40-41 0.5185541135227041 1107 42-43 1.0421576475372945 1107 44-45 0.9397421712037826 1107 46-47 -0.048244204627323484 1107 48-49 0.8017097589046855 1107 50-51 1.1421707545232458 1107 52-53 1.5547050443978314 1107 54-55 0.9351897156113935 1107 56-57 1.3962083636524945 1107 58-59 1.293412501946623 1107 60-61 -0.30222735979956283 1107 62-63 -0.8780025660304851 1107 64-65 0.41741170235305347 1107 66-67 1.3014219807735223 1107 68-69 1.0727934336858453 1107 70-71 0.5610854928738007 1107 72-73 0.9769246780880216 1107 74-75 0.9846898823225843 1107 76 -0.6173138488670098 1108 1 -0.4008380831217728 1108 2 0.024875347353638944 1108 3 -0.3200916293522731 1108 4 -0.11151262719599586 1108 5 -0.47254905824635784 1108 6 0.008840258554574376 1108 7 0.7535402108514262 1108 8 0.5724306154247287 1108 9 -1.5970439077450216 1108 10-11 -0.3129665130010366 1108 12-13 0.08630036189033419 1108 14-15 0.05467430017048258 1108 16-17 -1.256441955350713 1108 18-19 -0.7160830871987933 1108 20-21 0.3375285065225775 1108 22-23 -1.2392000437596913 1108 24-25 -1.3233783524561247 1108 26-27 -0.8133855156142253 1108 28-29 -0.15511410013158056 1108 30-31 0.4218367523053743 1108 32-33 0.3763756368814448 1108 34-35 0.5511103461819964 1108 36-37 -0.26515643849607784 1108 38-39 0.6113841194933407 1108 40-41 -0.7059793056185981 1108 42-43 -0.2971132911406542 1108 44-45 -0.22774426807175274 1108 46-47 0.2013420783551183 1108 48-49 -0.24662617931356934 1108 50-51 0.2926947960788233 1108 52-53 0.319598857029991 1108 54-55 0.3486768903838495 1108 56-57 0.9018854805045713 1108 58-59 -1.6930497578676622 1108 60-61 0.3723842058895066 1108 62-63 -0.43654352401522445 1108 64-65 -0.38922156049515877 1108 66-67 -0.31120098511780725 1108 68-69 0.5922611177899206 1108 70-71 1.577795063527475 1108 72-73 -0.14293833561060865 1108 74-75 0.7512845315824919 1108 76 0.9372508288934007 1109 1 -1.0156479308083313 1109 2 -1.506880567963922 1109 3 0.13411854403598156 1109 4 -0.2847092935433473 1109 5 0.5595421382284016 1109 6 0.5015598677125404 1109 7 -1.6685308687538978 1109 8 0.26904906546783636 1109 9 1.0638910477104844 1109 10-11 0.6539603957066937 1109 12-13 0.8129504241568313 1109 14-15 -0.0387972967257717 1109 16-17 -0.07024116983486195 1109 18-19 -0.5096217403264909 1109 20-21 0.994966958481573 1109 22-23 0.3718498652354256 1109 24-25 0.4033196071185472 1109 26-27 -0.17850669580389678 1109 28-29 -0.6487198094907107 1109 30-31 0.1323693703722384 1109 32-33 0.6632326155192452 1109 34-35 0.6037015905540635 1109 36-37 0.30850962147135164 1109 38-39 0.24980734010834027 1109 40-41 0.25237398492647145 1109 42-43 1.181548458069038 1109 44-45 0.19214748401177673 1109 46-47 -0.6365015848310094 1109 48-49 -0.5906964925619675 1109 50-51 0.6942897764409253 1109 52-53 0.3228798226397238 1109 54-55 -0.10510220723086405 1109 56-57 -0.218073327810405 1109 58-59 -0.1894335111007308 1109 60-61 1.6836927603487482 1109 62-63 -0.26677151520211595 1109 64-65 0.11295776702663574 1109 66-67 0.7637583021702454 1109 68-69 1.605552620807451 1109 70-71 -1.170528530686532 1109 72-73 -1.131466930303585 1109 74-75 1.5360579773148402 1109 76 -0.34505590546812925 1110 1 0.5074789423185422 1110 2 0.5457168346334811 1110 3 -0.20649084657340566 1110 4 -0.43675724559129847 1110 5 -0.17127704259078058 1110 6 -0.09888968273700982 1110 7 -0.0015478517708800155 1110 8 1.1098083062270767 1110 9 -0.8182767844573497 1110 10-11 -1.419815828069531 1110 12-13 -0.7756859394795299 1110 14-15 -0.5041319620604341 1110 16-17 0.11322536363167401 1110 18-19 1.2306879499832029 1110 20-21 0.373438486953102 1110 22-23 -0.0159123725268131 1110 24-25 0.42544747924641513 1110 26-27 -0.8378473551445573 1110 28-29 0.46866280789190284 1110 30-31 0.3412105292133951 1110 32-33 1.437383464670095 1110 34-35 0.3228324596849319 1110 36-37 0.3924756554373836 1110 38-39 0.7370201273211272 1110 40-41 0.4803709879234752 1110 42-43 0.4878671393877205 1110 44-45 1.0157488826131775 1110 46-47 1.018318594989168 1110 48-49 1.0969158950504223 1110 50-51 0.7023816845328348 1110 52-53 0.8977299724898735 1110 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2.683341475205772 2116 46-47 1.3885566902272686 2116 48-49 1.6772069003414316 2116 50-51 0.6314822136333653 2116 52-53 2.978020977780883 2116 54-55 0.9741842470555895 2116 56-57 1.5134405287034518 2116 58-59 0.17161284994563175 2116 60-61 -0.38332355666757323 2116 62-63 2.262657389226792 2116 64-65 2.2013416054104766 2116 66-67 1.0277766911886346 2116 68-69 1.4401809554357854 2116 70-71 -1.4835950937530953 2116 72-73 1.4584061595695026 2116 74-75 -1.063657122400258 2116 76 0.2179459075336858 2117 1 1.047012181356731 2117 2 1.5435244725401311 2117 3 1.950015518348799 2117 4 1.0715878746350107 2117 5 -0.39496869747055285 2117 6 -0.03191655685299466 2117 7 -0.06173172730129295 2117 8 -0.33601913365977154 2117 9 1.3406341066317609 2117 10-11 0.6480059541086902 2117 12-13 1.0829774268571057 2117 14-15 1.0695596928193396 2117 16-17 1.4603965093176718 2117 18-19 0.6538138340001716 2117 20-21 -0.0911301127640094 2117 22-23 1.346958914601899 2117 24-25 1.8277812840554688 2117 26-27 2.457060707090239 2117 28-29 2.1490022704095857 2117 30-31 1.4524552251115566 2117 32-33 2.394278797060476 2117 34-35 1.257132318242217 2117 36-37 -0.017496056019474793 2117 38-39 1.1848277652277766 2117 40-41 1.0432069143732612 2117 42-43 0.7645644519761206 2117 44-45 2.130352843009213 2117 46-47 0.8926821027685179 2117 48-49 1.020395385856645 2117 50-51 2.8909248245611217 2117 52-53 3.319971839533718 2117 54-55 1.0728732826258103 2117 56-57 1.5126612841123297 2117 58-59 1.0867210274300447 2117 60-61 1.5135411298010766 2117 62-63 0.5006936928571513 2117 64-65 1.29567603885382 2117 66-67 -0.5707289927130859 2117 68-69 1.414145018508762 2117 70-71 2.224784077497361 2117 72-73 2.9571226978899965 2117 74-75 1.0764235523338783 2117 76 0.5060830545817225 2118 1 1.086916171755771 2118 2 1.8466547855714346 2118 3 1.4332638431812832 2118 4 1.4270234181893642 2118 5 0.5937301724164357 2118 6 1.3003166664693389 2118 7 1.771251571028543 2118 8 -4.229008432589662 2118 9 -0.6369636531442175 2118 10-11 1.0564467981930932 2118 12-13 1.671536282742693 2118 14-15 -0.3787351366636109 2118 16-17 1.0245529249592344 2118 18-19 -0.6382153689201182 2118 20-21 -2.409461945947328 2118 22-23 1.11893611232167 2118 24-25 2.067655271454207 2118 26-27 1.5907240734268733 2118 28-29 1.3380711773002751 2118 30-31 -0.5781978401949743 2118 32-33 1.4200313723180038 2118 34-35 2.2513317381842093 2118 36-37 0.8951452081069355 2118 38-39 1.2202813105823118 2118 40-41 -0.4430417104892257 2118 42-43 1.2048584813790626 2118 44-45 1.6917589836232807 2118 46-47 1.5577486094191855 2118 48-49 -0.7196786215440945 2118 50-51 1.7308088129599604 2118 52-53 -1.1650766653167643 2118 54-55 1.4636623615337037 2118 56-57 2.2360836263465522 2118 58-59 2.4527576310904102 2118 60-61 -0.9010003243443379 2118 62-63 1.9962432478126466 2118 64-65 1.4664931205619922 2118 66-67 -0.17718963877769411 2118 68-69 3.155669170924007 2118 70-71 0.9499065897485899 2118 72-73 -0.7553985542352102 2118 74-75 1.636679577936441 2118 76 0.7070031465909246 2119 1 -0.0799207366541701 2119 2 0.06104588760298313 2119 3 -0.15055184175799852 2119 4 1.1067419518404833 2119 5 1.1812911757077735 2119 6 0.11804450666909005 2119 7 -0.9404724103583604 2119 8 2.3444792532575747 2119 9 0.1643074852055726 2119 10-11 -0.1091416707661601 2119 12-13 0.8032466447901356 2119 14-15 -0.05726197811179645 2119 16-17 -0.01590786269256128 2119 18-19 -0.5143361270633484 2119 20-21 -1.1988729095155968 2119 22-23 1.0610539196080175 2119 24-25 -0.2173294067888918 2119 26-27 0.36038699477518676 2119 28-29 0.4895295815676697 2119 30-31 -0.2746000646870854 2119 32-33 0.021211715071377313 2119 34-35 0.9094295704393467 2119 36-37 0.0736153023073669 2119 38-39 0.4832480566951247 2119 40-41 -0.17400300886625075 2119 42-43 -1.218192250660433 2119 44-45 -1.1508241510946888 2119 46-47 -0.2022672798101901 2119 48-49 0.446193583653951 2119 50-51 0.9627348145488845 2119 52-53 -1.546531664749292 2119 54-55 0.012521741853756652 2119 56-57 0.6217946693609626 2119 58-59 0.34993161815203777 2119 60-61 0.9434554695496544 2119 62-63 0.39233903716686314 2119 64-65 -0.09797175289164883 2119 66-67 0.6794100661366187 2119 68-69 -0.1592780541355836 2119 70-71 -0.7165330879270435 2119 72-73 -1.2421764455074857 2119 74-75 -0.4146766812849876 2119 76 1.2740216461936917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 20.0 13 153.0 14 466.0 15 861.0 16 1224.0 17 1366.0 18 1463.0 19 1369.0 20 1177.0 21 968.0 22 800.0 23 657.0 24 615.0 25 647.0 26 683.0 27 824.0 28 927.0 29 1184.0 30 1459.0 31 1779.0 32 2291.0 33 2846.0 34 3857.0 35 5280.0 36 8006.0 37 6502.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.868024612773176 21.822618289836623 15.279015489072778 29.03034160831742 2 17.237380339897946 22.74912495255767 30.643528866022855 29.369965841521527 3 18.949521359591785 24.9377978324126 28.153333614473077 27.95934719352254 4 11.462067220511955 24.65946948930966 33.55754227638848 30.320921013789903 5 11.481044152996246 32.86804706279256 31.668283220174587 23.982625564036606 6 24.49289419305866 35.946527221355375 17.89103023657909 21.669548349006874 7 22.17770842997512 30.377851811242778 19.73811833171678 27.706321427065323 8 19.49774385358242 32.454771644245774 18.44768692278497 29.599797579386834 9 20.31796904651457 20.400202420613166 19.71281575507106 39.569012777801206 10-11 20.481381520684856 26.246151899801795 26.073250959389366 27.199215620123983 12-13 20.35170581537553 24.517142495677476 25.80757390460929 29.323577784337708 14-15 19.557837473116013 26.13229030489605 23.579892885758866 30.729979336229075 16-17 21.16771391220006 28.254543921055962 24.39590098258339 26.181841184160586 18-19 19.31640872095475 27.625142326993636 25.026356850672627 28.032092101378993 20-21 18.84409395690128 27.804368911567494 25.081179100071694 28.270358031459537 22-23 21.54619828785898 27.532366212625988 24.598321595749166 26.323113903765865 24-25 20.29477501792266 29.404756884409395 24.312613334457893 25.987854763210056 26-27 18.693332771053853 29.79378400033737 25.82127946695905 25.691603761649727 28-29 20.047020621599966 28.288280689916924 27.07059418884156 24.59410449964155 30-31 19.826677349976805 28.11959684561211 27.18867287985493 24.86505292455615 32-33 18.17568422384346 30.209167966937965 26.284105764770377 25.331042044448193 34-35 19.456627166533124 29.00518702821237 26.499177666259015 25.039008138995488 36-37 19.49774385358242 28.29671488213216 26.370556234976593 25.834985029308815 38-39 19.223632606587103 27.463838400877155 27.875005271370135 25.437523721165604 40-41 19.264749293636402 27.048454434276557 27.31307721502973 26.37371905705731 42-43 17.9764264327584 26.889259056213895 28.22502424830262 26.90929026272509 44-45 17.69493526757475 26.856576561379836 28.684687724033232 26.763800447012187 46-47 18.461392485134738 27.471218319065493 27.65149917766626 26.415890018133513 48-49 17.546282629781132 28.13646523004259 27.547126049002657 26.77012609117362 50-51 17.620081811664488 27.79382617129844 27.07375701092228 27.512335006114792 52-53 16.871547252561886 27.531311938599078 27.969889933791592 27.627250875047444 54-55 16.638552692615864 27.94880445325349 26.980980896554634 28.431661957576015 56-57 17.330156454265595 27.733732551764856 27.08008265508371 27.856028338885842 58-59 17.412389828364187 27.186564331801122 26.85973938346055 28.541306456374137 60-61 17.123518744992197 26.7458777885548 26.857630835406738 29.27297263104626 62-63 17.35862185299203 27.263526335765192 26.578248218276894 28.799603592965884 64-65 18.034411504238182 27.899253573988954 25.14759836376671 28.91873655800616 66-67 18.034411504238182 28.642516762957026 24.172394888879516 29.15067684392527 68-69 17.88681314047147 28.204993041791422 24.606755787964406 29.301438029772697 70-71 18.898916206300342 28.538143634293426 24.10175852907688 28.461181630329357 72-73 18.607936574874543 27.927718972715386 23.398557753131193 30.065786699278878 74-75 18.336988149959936 28.369459789988618 23.413317589507866 29.88023447054358 76 18.938978619322736 26.75325770674314 23.5862185299203 30.721545144013835 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 0.5 11 0.0 12 0.0 13 0.5 14 3.0 15 2.5 16 1.5 17 3.0 18 8.0 19 14.0 20 18.0 21 21.0 22 23.0 23 41.0 24 61.0 25 65.0 26 92.5 27 151.0 28 224.0 29 266.0 30 321.0 31 430.5 32 562.5 33 640.0 34 758.0 35 973.0 36 1199.5 37 1329.0 38 1495.5 39 1878.0 40 2361.0 41 2720.0 42 2812.0 43 2886.0 44 3163.5 45 3388.5 46 3410.0 47 3291.5 48 3207.5 49 3111.0 50 2980.0 51 2818.0 52 2468.5 53 2105.0 54 1929.0 55 1741.0 56 1444.5 57 1148.5 58 961.0 59 846.5 60 634.5 61 490.5 62 444.0 63 371.0 64 272.0 65 204.5 66 134.0 67 105.0 68 91.5 69 69.0 70 49.0 71 38.0 72 32.0 73 24.5 74 17.0 75 11.0 76 13.5 77 12.5 78 5.5 79 2.0 80 3.5 81 6.0 82 4.5 83 2.0 84 3.0 85 3.0 86 2.5 87 3.0 88 2.0 89 2.0 90 2.0 91 1.5 92 2.0 93 1.0 94 2.0 95 2.0 96 0.0 97 1.5 98 2.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6241302239278033 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 47426.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.41534179563952 #Duplication Level Percentage of deduplicated Percentage of total 1 84.89173362385945 65.71922574115464 2 8.72123110445322 13.503141736600178 3 3.0695900857959963 7.129000969932105 4 1.5633937082936131 4.8412263315480955 5 0.7789731717281765 3.015223716948509 6 0.435789186980798 2.0242061316577407 7 0.1961051341413591 1.0627082191203139 8 0.10622361432656952 0.6578669927887656 9 0.08171047255889964 0.5693079745287395 >10 0.1552498978619093 1.4780921857209126 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010542740269050731 0.0 2 0.0 0.0 0.0 0.010542740269050731 0.0 3 0.0 0.0 0.0 0.010542740269050731 0.0 4 0.0 0.0 0.0 0.010542740269050731 0.0 5 0.0 0.0 0.0 0.010542740269050731 0.0 6 0.0 0.0 0.0 0.010542740269050731 0.0 7 0.0 0.0 0.0 0.010542740269050731 0.0 8 0.0 0.0 0.0 0.010542740269050731 0.0 9 0.0 0.0 0.0 0.01686838443048117 0.0 10 0.0 0.0 0.0 0.018976932484291317 0.0 11 0.0 0.0 0.0 0.018976932484291317 0.0 12 0.0 0.0 0.0 0.018976932484291317 0.0 13 0.0 0.0 0.0 0.021085480538101463 0.0 14 0.0 0.0 0.0 0.02319402859191161 0.0 15 0.0 0.0 0.0 0.02319402859191161 0.0 16 0.0 0.0 0.0 0.02319402859191161 0.0 17 0.0 0.0 0.0 0.027411124699531903 0.0 18 0.0 0.0 0.0 0.0316282208071522 0.0 19 0.0 0.0 0.0 0.037953864968582635 0.0 20 0.0 0.0 0.0 0.04006241302239278 0.0 21 0.0 0.0 0.0 0.04849660523763336 0.0 22 0.0 0.0 0.0 0.050605153291443515 0.0 23 0.0 0.0 0.0 0.06114789356049424 0.0 24 0.0 0.0 0.0 0.06114789356049424 0.0 25 0.0 0.0 0.0 0.06114789356049424 0.0 26 0.0 0.0 0.0 0.0632564416143044 0.0 27 0.0 0.0 0.0 0.086450470206216 0.0 28 0.0 0.0 0.0 0.10331885463669717 0.0 29 0.0 0.0 0.0 0.12018723906717835 0.0 30 0.0 0.0 0.0 0.1454898157129001 0.0 31 0.0 0.0 0.0 0.17500948846624215 0.0 32 0.0 0.0 0.0 0.2045291612195842 0.0 33 0.0 0.0 0.0 0.24459157424197697 0.0 34 0.0 0.0 0.0 0.29308817947961036 0.0 35 0.0 0.0 0.0 0.3479104288786741 0.0 36 0.0 0.0 0.0 0.4343608990848901 0.0 37 0.0 0.0 0.0 0.6114789356049425 0.0 38 0.0 0.0 0.0 0.8729388942774006 0.0 39 0.0 0.0 0.0 1.2313920634251254 0.0 40 0.0 0.0 0.0 1.644667481971914 0.0 41 0.0 0.0 0.0 2.16337030320921 0.0 42 0.0 0.0 0.0 2.781174882975583 0.0 43 0.0 0.0 0.0 3.4664530004638805 0.0 44 0.0 0.0 0.0 4.0716062919073925 0.0 45 0.0 0.0 0.0 4.687302323619956 0.0 46 0.0 0.0 0.0 5.300889807278708 0.0 47 0.0 0.0 0.0 5.893391810399359 0.0 48 0.0 0.0 0.0 6.435288660228567 0.0 49 0.0 0.0 0.0 7.025682115295408 0.0 50 0.0 0.0 0.0 7.519082359886982 0.0 51 0.0 0.0 0.0 8.189600640998608 0.0 52 0.0 0.0 0.0 8.952895036477882 0.0 53 0.0 0.0 0.0 9.718297980010965 0.0 54 0.0 0.0 0.0 10.456289798844516 0.0 55 0.0 0.0 0.0 11.08042002277232 0.0 56 0.0 0.0 0.0 11.761481044152996 0.0 57 0.0 0.0 0.0 12.25277274069076 0.0 58 0.0 0.0 0.0 12.746172985282335 0.0 59 0.0 0.0 0.0 13.248007422089149 0.0 60 0.0 0.0 0.0 13.865812001855522 0.0 61 0.0 0.0 0.0 14.390840467254248 0.0 62 0.0 0.0 0.0 14.97069118205204 0.0 63 0.0 0.0 0.0 15.512588031881247 0.0 64 0.0 0.0 0.0 16.13039261164762 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTCA 15 0.0022119964 69.997894 8 TCGACGC 25 2.343365E-4 56.116703 1 TTAGCTT 20 0.0069120177 52.498417 10 CACTGAG 20 0.0069120177 52.498417 7 TCTGTTC 20 0.0069120177 52.498417 8 ATTGACC 20 0.0069120177 52.498417 46 TGGCTGG 20 0.0069120177 52.498417 33 CGACGCC 40 0.0023965414 34.998943 2 ATAACCA 40 0.0023965414 34.998943 46 TTTCCTA 45 0.0042679445 31.110176 40 TCGACCC 50 0.0070695584 28.058352 1 GAGGCAA 95 9.154412E-6 25.788696 70 CGAGGCA 120 5.5723165E-5 20.416052 69 ACGAGGC 180 6.9244E-5 15.555088 70 GACGAGG 215 1.8981329E-5 14.6507225 69 CGACGAG 225 2.9151017E-5 13.999578 68 CCGACGA 250 0.0011152134 11.199663 67 GCCGACG 305 4.977379E-4 10.327558 66 TGCCGAC 300 0.0050705103 9.333053 65 GCTGCCG 405 6.78484E-4 8.641714 70 >>END_MODULE