FastQCFastQC Report
Wed 25 May 2016
SRR1780402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47426
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT19414.092691772445494TruSeq Adapter, Index 8 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT16853.5529034706700964No Hit
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC7401.5603255598195083No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC3110.6557584447349555No Hit
TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT1590.33525914055581324No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG1230.259351410618648No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT1200.2530257664572176No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT940.19820351705815376No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC880.1855522287352929No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG850.17922658457386245No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT750.15814110403576098No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT670.14127271960527982No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT630.13283852739003923No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG590.12440433517479864No Hit
CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC570.12018723906717835No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC550.11597014295955804No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC500.10542740269050732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG150.002211731670.0000140
AGCAAGG150.002211731670.000015
CGTCTGC150.002211731670.0000138
CTGACCT150.002211731670.0000125
TAGCAAG150.002211731670.000014
TACTGAC150.002211731670.000013
CTGCCGT150.002211731670.0000141
TTGGTAA150.002211731670.0000129
GAACAAA150.002211731670.0000159
CTTAATA252.3676449E-455.9999961
TTAATAC252.3676449E-455.9999962
TGGTCTA252.3676449E-455.99999643
ACGCGAC200.006911192652.522
GCGACGG200.006911192652.524
CTCGTCT200.006911192652.536
TAAAGTG200.006911192652.568
GTCTGCC200.006911192652.539
CGACGGC200.006911192652.525
CGCGACG200.006911192652.523
ACAGGCC200.006911192652.58
Produced by FastQC (version 0.11.5)