##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780402_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47426 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.496183528022605 34.0 34.0 34.0 31.0 34.0 2 33.096002192889976 34.0 34.0 34.0 32.0 34.0 3 33.33897018513052 34.0 34.0 34.0 33.0 34.0 4 33.391072407540165 34.0 34.0 34.0 33.0 34.0 5 33.41061864799899 34.0 34.0 34.0 34.0 34.0 6 37.131531227596675 38.0 37.0 38.0 36.0 38.0 7 36.69329060009277 38.0 37.0 38.0 34.0 38.0 8 36.67290094041243 38.0 37.0 38.0 34.0 38.0 9 36.457196474507654 38.0 37.0 38.0 34.0 38.0 10-11 36.08306625057985 38.0 36.5 38.0 32.5 38.0 12-13 35.93675410112597 38.0 36.0 38.0 32.0 38.0 14-15 36.22113397714334 38.0 37.0 38.0 33.0 38.0 16-17 36.342986969173026 38.0 37.0 38.0 34.0 38.0 18-19 36.38447265213174 38.0 37.0 38.0 34.0 38.0 20-21 36.04322523510311 38.0 37.0 38.0 32.5 38.0 22-23 36.09407287142074 38.0 36.5 38.0 32.5 38.0 24-25 35.2029899211403 37.5 35.0 38.0 29.0 38.0 26-27 35.44148779150677 37.5 36.0 38.0 31.0 38.0 28-29 35.257379918188334 38.0 36.0 38.0 29.0 38.0 30-31 34.94419727575591 37.0 35.5 38.0 29.0 38.0 32-33 35.47391726057437 38.0 36.0 38.0 31.0 38.0 34-35 35.276124910386706 38.0 36.0 38.0 31.0 38.0 36-37 35.31438451482309 38.0 36.0 38.0 31.0 38.0 38-39 34.76636233289757 37.5 35.0 38.0 26.0 38.0 40-41 34.59309239657571 37.0 34.5 38.0 26.5 38.0 42-43 35.30296672711171 37.0 35.5 38.0 29.0 38.0 44-45 35.55601357904946 38.0 36.0 38.0 31.0 38.0 46-47 35.84290262725088 38.0 36.5 38.0 32.0 38.0 48-49 35.53646733859065 38.0 36.0 38.0 31.0 38.0 50-51 35.66406612406697 38.0 36.0 38.0 31.0 38.0 52-53 35.51967275334205 38.0 36.0 38.0 31.0 38.0 54-55 35.744233121072824 38.0 36.5 38.0 31.5 38.0 56-57 35.82042550499726 38.0 37.0 38.0 31.5 38.0 58-59 35.833793699658415 38.0 37.0 38.0 32.0 38.0 60-61 35.53653059503226 38.0 36.5 38.0 29.5 38.0 62-63 35.96106566018639 38.0 36.5 38.0 33.0 38.0 64-65 35.9226057436849 38.0 37.0 38.0 32.5 38.0 66-67 34.95470838780416 37.5 35.5 38.0 27.5 38.0 68-69 33.62076708978198 37.0 34.0 38.0 22.5 38.0 70-71 32.88382954497533 37.0 32.5 38.0 16.0 38.0 72-73 32.15393455066841 37.0 30.0 38.0 11.0 38.0 74-75 32.57135326614093 37.0 32.0 38.0 11.0 38.0 76 32.165837304432166 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8426405896928131 1101 2 0.4920209723416633 1101 3 0.02110214751149897 1101 4 0.3237427134729245 1101 5 0.10579984859882074 1101 6 -1.130064298355542 1101 7 0.1979619988676049 1101 8 0.6452174856430446 1101 9 0.6073805451530276 1101 10-11 -0.2614698599541825 1101 12-13 0.9719593487620841 1101 14-15 0.25554483948976525 1101 16-17 -0.5299226099321359 1101 18-19 -0.670916045083608 1101 20-21 -0.45631225784692475 1101 22-23 0.539928355825289 1101 24-25 -3.255957847551013 1101 26-27 -0.3370001582160356 1101 28-29 0.8624391959098858 1101 30-31 0.7924939511220188 1101 32-33 0.16641879435912443 1101 34-35 0.5376793019288115 1101 36-37 -0.05128988144878832 1101 38-39 -0.5348827097384543 1101 40-41 1.0210712201908052 1101 42-43 0.23978337341874578 1101 44-45 0.6748044233299595 1101 46-47 1.1511212650646812 1101 48-49 -1.6335219409079471 1101 50-51 -1.4940645271838235 1101 52-53 -1.94076190025406 1101 54-55 -1.3494555926939782 1101 56-57 -1.9833885281840438 1101 58-59 -1.7221276206658018 1101 60-61 -2.0443037519015803 1101 62-63 -1.4268168559864591 1101 64-65 -2.327010131403803 1101 66-67 -1.7999159924948032 1101 68-69 -2.109410982986617 1101 70-71 -1.9430765055635355 1101 72-73 -1.8282545303023738 1101 74-75 -1.1538591392682989 1101 76 -0.8465309362853759 1102 1 -0.8742128183370639 1102 2 0.09974739624455964 1102 3 0.5751404294909577 1102 4 0.3279443941451916 1102 5 0.35579984859882074 1102 6 0.33492169604221544 1102 7 0.5120376291197033 1102 8 0.8539009590324014 1102 9 0.4121191072445285 1102 10-11 0.8314923249197648 1102 12-13 1.07466709852865 1102 14-15 0.31553550242160355 1102 16-17 0.05627020052537546 1102 18-19 -0.5108436828053655 1102 20-21 0.8966289186236693 1102 22-23 0.5752925014835526 1102 24-25 -5.5431894068413925 1102 26-27 -0.9081369462645839 1102 28-29 0.18071650683425844 1102 30-31 2.0445364347821453 1102 32-33 0.41828620799123684 1102 34-35 1.0033072197626112 1102 36-37 -1.6402838123544754 1102 38-39 0.44212475991606937 1102 40-41 1.5648970838696101 1102 42-43 0.7680280046045596 1102 44-45 1.320345973283267 1102 46-47 1.6004676702934404 1102 48-49 -3.4845957037464146 1102 50-51 -0.25345995202042815 1102 52-53 0.5569038327057925 1102 54-55 0.8845546780809954 1102 56-57 1.5866394830204413 1102 58-59 0.7689438079056288 1102 60-61 -5.9534424073637595 1102 62-63 -2.3325124675644275 1102 64-65 0.3882676463739756 1102 66-67 1.8061180878039806 1102 68-69 -0.1600062210818578 1102 70-71 -0.44401021237960236 1102 72-73 -4.95821484776269 1102 74-75 -1.0576289805381407 1102 76 0.18626551562872606 1103 1 0.750190997243223 1103 2 0.5125057428264341 1103 3 -0.019652893243545577 1103 4 0.4308855706157786 1103 5 0.26120525400422423 1103 6 0.09644792815668524 1103 7 0.6139851648907708 1103 8 0.6333127237382854 1103 9 0.08560877338125295 1103 10-11 -0.12150846999278997 1103 12-13 -0.317026065223331 1103 14-15 -0.6272307932858681 1103 16-17 0.36459690958737667 1103 18-19 0.7939059197383571 1103 20-21 -0.36171766325232824 1103 22-23 0.18101587191280544 1103 24-25 -3.300144891738057 1103 26-27 -0.5801361513520291 1103 28-29 -0.5670974836267959 1103 30-31 0.6682447018727657 1103 32-33 0.985058862999189 1103 34-35 -0.38113236688285923 1103 36-37 -1.474445179604082 1103 38-39 -1.7482567981125499 1103 40-41 -0.5200591959396093 1103 42-43 -0.6443328856975086 1103 44-45 1.6460613945869227 1103 46-47 1.296016159959585 1103 48-49 -3.0108278182138264 1103 50-51 -0.5691396022589004 1103 52-53 0.2065461220539646 1103 54-55 -0.568942937181319 1103 56-57 0.09737080257528419 1103 58-59 -0.7563940299322098 1103 60-61 -4.457913790511615 1103 62-63 -1.0381960923656948 1103 64-65 0.0386054842118142 1103 66-67 -1.6896092571880672 1103 68-69 0.09506134648570708 1103 70-71 0.20165751417048483 1103 72-73 -5.385579712627553 1103 74-75 -2.410669521078681 1103 76 -2.7626613524157904 1104 1 -0.061360280061478534 1104 2 0.6516208853662349 1104 3 0.429995385171857 1104 4 -0.5862377170554538 1104 5 0.21538888969470804 1104 6 0.2499096090156243 1104 7 0.2830892004331602 1104 8 0.6641346415465037 1104 9 1.1888112909673367 1104 10-11 -0.16704715936710102 1104 12-13 0.6050861154504901 1104 14-15 0.6515548416641437 1104 16-17 0.37723111913939533 1104 18-19 0.8704516435443495 1104 20-21 0.2834137695503358 1104 22-23 0.5373190929420488 1104 24-25 0.3459121241819787 1104 26-27 1.0612929489811833 1104 28-29 1.7636133642073446 1104 30-31 1.4298607699956847 1104 32-33 0.6185279485230737 1104 34-35 0.7750135050164388 1104 36-37 1.5740090965899185 1104 38-39 1.7202270967412119 1104 40-41 2.493130363482827 1104 42-43 1.1862391246690223 1104 44-45 0.7507448451607885 1104 46-47 1.0773008693264785 1104 48-49 0.4350255694203824 1104 50-51 0.9781576950383979 1104 52-53 1.0069441227944296 1104 54-55 0.5967827199826914 1104 56-57 0.8217321502242996 1104 58-59 -0.427117835929991 1104 60-61 1.0496297118949087 1104 62-63 0.8242159772381541 1104 64-65 1.032959427195891 1104 66-67 0.8531064036782823 1104 68-69 1.929891039192114 1104 70-71 1.418197684477775 1104 72-73 0.8645591248400493 1104 74-75 -0.12869062437376044 1104 76 -0.4429649436782874 1105 1 1.2746842404864651 1105 2 0.3196003374210292 1105 3 0.4497727824321345 1105 4 0.29963557061577717 1105 5 -0.025450151401180676 1105 6 0.16874522545398207 1105 7 0.8381405702961757 1105 8 -0.6291872762617174 1105 9 0.6393249895974691 1105 10-11 0.83094085433153 1105 12-13 -0.009340254412521176 1105 14-15 0.12766785536278036 1105 16-17 0.1391746122900912 1105 18-19 1.0654430819005185 1105 20-21 0.6405627421530724 1105 22-23 0.23650573677766573 1105 24-25 0.9095679460997843 1105 26-27 0.5924145243236509 1105 28-29 1.2285106244813164 1105 30-31 1.6858967288997917 1105 32-33 1.2980656197559455 1105 34-35 -0.24228101553150339 1105 36-37 1.0383926582337537 1105 38-39 0.43366887756312167 1105 40-41 0.7389779662225493 1105 42-43 1.1832853575457278 1105 44-45 1.4666695026950336 1105 46-47 1.3747323761757926 1105 48-49 0.2199998844888782 1105 50-51 1.1719076950383993 1105 52-53 0.9087420679999028 1105 54-55 1.1018340898457026 1105 56-57 0.7221174241969024 1105 58-59 0.5658188079056288 1105 60-61 1.2195355338127172 1105 62-63 -0.09740217344678115 1105 64-65 1.4226426463739728 1105 66-67 1.5515224995686907 1105 68-69 0.6587437789181436 1105 70-71 1.8504750817380469 1105 72-73 1.076160152237307 1105 74-75 -0.1357539805381407 1105 76 1.1370008097463682 1106 1 -0.7792930322408083 1106 2 0.34194882226951506 1106 3 0.1029924794018271 1106 4 0.4384613281915364 1106 5 0.25731500011396946 1106 6 0.27253310424185884 1106 7 -0.8442457933401926 1106 8 0.42119151161707435 1106 9 -0.3724174346449516 1106 10-11 0.6649370664527439 1106 12-13 1.6490877758905071 1106 14-15 0.237326946271871 1106 16-17 0.5483602183506946 1106 18-19 0.9181892940217296 1106 20-21 0.657324105789435 1106 22-23 0.6973958882928244 1106 24-25 0.20435961276644576 1106 26-27 1.2432667970509215 1106 28-29 1.4160106244813164 1106 30-31 1.6005747592028285 1106 32-33 1.0069671349074625 1106 34-35 1.7732492874987926 1106 36-37 1.5830896279307254 1106 38-39 0.8743885745328299 1106 40-41 2.0701332692528567 1106 42-43 1.170880054515436 1106 44-45 1.4170482905738169 1106 46-47 1.239505103448522 1106 48-49 1.7297536723676643 1106 50-51 0.5766425435232421 1106 52-53 1.379764795272635 1106 54-55 0.2952053019669165 1106 56-57 0.7606590908635695 1106 58-59 1.11174683820866 1106 60-61 1.1429256853278602 1106 62-63 1.221631917462318 1106 64-65 -1.3863535657472426 1106 66-67 0.48002628744747966 1106 68-69 1.8305240819484396 1106 70-71 0.13664932416229192 1106 72-73 1.1611033340554897 1106 74-75 -0.06709867750783616 1106 76 0.4904099006554645 1107 1 1.1914208176676766 1107 2 0.3873016797029081 1107 3 0.03857311800260277 1107 4 0.4258520135688002 1107 5 -0.01836122522668404 1107 6 0.46421502411170223 1107 7 0.61838385888678 1107 8 -0.01937184002010639 1107 9 -0.4079065540266882 1107 10-11 0.996440015405355 1107 12-13 0.028180718741836586 1107 14-15 0.1002769157654626 1107 16-17 0.8600848471894125 1107 18-19 1.0986855315649464 1107 20-21 1.2349629099383108 1107 22-23 0.3134981864420965 1107 24-25 0.5930620736165579 1107 26-27 -0.3541249052065538 1107 28-29 1.5158428392464174 1107 30-31 0.8420838094367085 1107 32-33 1.7334263580109806 1107 34-35 0.6094807294349422 1107 36-37 1.5236275575626124 1107 38-39 0.990338340650375 1107 40-41 1.0439486038064416 1107 42-43 1.2205546528477456 1107 44-45 1.0981292342386624 1107 46-47 1.4904202956388772 1107 48-49 1.3714050858311566 1107 50-51 1.5385603796021528 1107 52-53 1.6885658935032666 1107 54-55 1.5142921435369843 1107 56-57 1.564630008089516 1107 58-59 1.1191954857579844 1107 60-61 1.686041406295928 1107 62-63 1.3010248399760442 1107 64-65 1.1739220087900861 1107 66-67 0.31966427809218345 1107 68-69 1.2716212956966686 1107 70-71 0.1945606522078478 1107 72-73 1.3677079710292617 1107 74-75 -0.1662699201354556 1107 76 0.5035276553839552 1108 1 -0.2394424718423025 1108 2 0.2886071867361011 1108 3 0.12177620708966685 1108 4 0.32472118705413777 1108 5 0.20169025955772213 1108 6 -0.33228217180629116 1108 7 -0.14156833381341727 1108 8 -0.4454543995493836 1108 9 -0.06461336656690975 1108 10-11 0.30555558035892716 1108 12-13 1.0742641976422718 1108 14-15 0.3227877183764747 1108 16-17 -0.09537162058662574 1108 18-19 0.4560680819005185 1108 20-21 1.1532767832489625 1108 22-23 0.4619766271886263 1108 24-25 1.291117603634028 1108 26-27 -0.041446777046211025 1108 28-29 -0.10282499195704276 1108 30-31 0.559997756297058 1108 32-33 1.221267674550468 1108 34-35 0.7955614502219177 1108 36-37 1.6425022472748552 1108 38-39 1.9257065487960006 1108 40-41 0.7431303634828268 1108 42-43 -0.27951429998851296 1108 44-45 0.7473201876265421 1108 46-47 1.2896296364497672 1108 48-49 1.3494091310642204 1108 50-51 1.7247330375041514 1108 52-53 0.6302317940273028 1108 54-55 -0.043628238921421314 1108 56-57 0.41762256118320806 1108 58-59 0.8811013421522063 1108 60-61 1.5188077940866833 1108 62-63 1.1461337854573372 1108 64-65 0.895973125826032 1108 66-67 1.4421474995686907 1108 68-69 1.7381102172743041 1108 70-71 0.2880606981764018 1108 72-73 0.1248330974427887 1108 74-75 -0.03622487094909843 1108 76 0.9748432754997935 1109 1 -1.2408314937792682 1109 2 -0.09394861362792284 1109 3 0.4672115936209451 1109 4 0.4501163398465451 1109 5 -0.17741693461795904 1109 6 -0.25310792139916316 1109 7 -0.1781618772562723 1109 8 0.46722880765437225 1109 9 0.8035732413457239 1109 10-11 0.38288578440146637 1109 12-13 0.618784745587476 1109 14-15 0.7993898833348041 1109 16-17 0.7587331787236522 1109 18-19 0.4892848651173054 1109 20-21 1.169561868027202 1109 22-23 0.36144017733710854 1109 24-25 0.116364274771108 1109 26-27 0.7598751886593149 1109 28-29 -0.09360476013407037 1109 30-31 0.5213206799487438 1109 32-33 0.48482261276294025 1109 34-35 -0.3322285679790582 1109 36-37 0.8642667841078833 1109 38-39 0.7429783181225673 1109 40-41 0.5171029662225521 1109 42-43 0.9057285393639134 1109 44-45 1.0391345376600682 1109 46-47 0.9160785300219487 1109 48-49 1.0362804788944686 1109 50-51 0.0795562964369978 1109 52-53 -0.3501303096224717 1109 54-55 -0.7333494765878541 1109 56-57 -0.0012872960828147484 1109 58-59 -0.006842905381084563 1109 60-61 1.3480538904560717 1109 62-63 0.6141144349448382 1109 64-65 1.0679005135068422 1109 66-67 0.052287359708550696 1109 68-69 -0.894709018284658 1109 70-71 0.35751179502476305 1109 72-73 -0.01984072188856345 1109 74-75 -0.6454786308877942 1109 76 -0.28931037906481905 1110 1 -1.1922800452278217 1110 2 -0.8714710911503971 1110 3 -0.2939772175678712 1110 4 -0.26911442938422425 1110 5 0.16294270574167768 1110 6 -0.06339763168887202 1110 7 0.583676284581891 1110 8 0.7904555808811438 1110 9 1.1161107038831872 1110 10-11 -0.6436127170970423 1110 12-13 0.5544990313017593 1110 14-15 0.8428464267913469 1110 16-17 0.6530138980043745 1110 18-19 -0.3117890609566203 1110 20-21 0.8865448850102169 1110 22-23 -0.36840497750804246 1110 24-25 -0.6373070539002157 1110 26-27 1.613396667180787 1110 28-29 0.6410106244813178 1110 30-31 -0.11187112824305956 1110 32-33 0.7061013340416622 1110 34-35 1.073790413039923 1110 36-37 1.281249801090901 1110 38-39 1.230990306134558 1110 40-41 -0.5078970337774535 1110 42-43 0.5060532146885919 1110 44-45 0.964883788409324 1110 46-47 0.8550895190329371 1110 48-49 1.456160598774595 1110 50-51 -0.6646994478187409 1110 52-53 0.7382063537141974 1110 54-55 -0.31334448158286676 1110 56-57 1.1368495670540497 1110 58-59 -0.7828419063800851 1110 60-61 0.32712481952700045 1110 62-63 -0.9228486020182061 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-2.69633396987215 2116 60-61 -2.628959836557655 2116 62-63 0.13998208581248406 2116 64-65 -2.0631212425149172 2116 66-67 -0.7893339819127903 2116 68-69 -0.421580295155934 2116 70-71 1.593299155812126 2116 72-73 0.5903962633484241 2116 74-75 0.35672287131370695 2116 76 0.48283414307970673 2117 1 0.4241273097933984 2117 2 0.2927439017774631 2117 3 0.0619633764915406 2117 4 0.03732121418013179 2117 5 -0.21598232961900266 2117 6 0.33928977990942855 2117 7 0.5763212633654788 2117 8 0.19519391185709623 2117 9 -1.4283730302045115 2117 10-11 -0.7798574129951987 2117 12-13 -1.9653736702541096 2117 14-15 0.10310102367960639 2117 16-17 -1.5106706847396225 2117 18-19 -2.194922017109384 2117 20-21 -0.17908453507464372 2117 22-23 0.4555342021242055 2117 24-25 -2.3261684400388347 2117 26-27 -2.9143614657753645 2117 28-29 -1.9664151180929395 2117 30-31 -0.41982728100119004 2117 32-33 -2.800263588164846 2117 34-35 0.2895259151615619 2117 36-37 -1.4002212031523769 2117 38-39 0.6984584815235237 2117 40-41 -0.2848772317972532 2117 42-43 -0.36519855334535123 2117 44-45 -0.8621671309683308 2117 46-47 -0.6585597030321253 2117 48-49 -0.7016894719467714 2117 50-51 -0.4624363643675409 2117 52-53 0.6215514739405066 2117 54-55 -0.25602482104537927 2117 56-57 0.4813686618206674 2117 58-59 -1.808407677242883 2117 60-61 -1.4739360008407516 2117 62-63 -1.207148460575489 2117 64-65 -0.9477472051111704 2117 66-67 -0.8375554707283399 2117 68-69 0.17477100664090983 2117 70-71 -0.03894323509363318 2117 72-73 1.2552752512472125 2117 74-75 1.5098215145113585 2117 76 2.2262334830137007 2118 1 -0.14292939587716802 2118 2 0.2914437717644631 2118 3 -1.3136741872648372 2118 4 -0.1574982677680623 2118 5 -2.6075746178833015E-4 2118 6 0.06546239717115299 2118 7 0.6229259238315308 2118 8 0.6964440368696003 2118 9 -1.3774679396954568 2118 10-11 -1.6431437416280659 2118 12-13 -1.6135384867357558 2118 14-15 0.33707442101934504 2118 16-17 -3.080427660437188 2118 18-19 -0.7080733322408932 2118 20-21 -1.1078274093620806 2118 22-23 0.4865373024342361 2118 24-25 0.2624404208472555 2118 26-27 -3.5559756271915006 2118 28-29 -0.8706055371348498 2118 30-31 -1.3186171599890883 2118 32-33 -0.5283864004460739 2118 34-35 -0.3656396013900931 2118 36-37 -3.701001281160181 2118 38-39 0.049893625037874756 2118 40-41 0.0524565015760885 2118 42-43 1.1229002565356296 2118 44-45 -2.632194133668605 2118 46-47 -0.060999947056522785 2118 48-49 0.6034910461050345 2118 50-51 -0.9864887696080658 2118 52-53 -2.2654372249293857 2118 54-55 -0.2729765162149036 2118 56-57 0.37050757571206105 2118 58-59 -0.6874955860337622 2118 60-61 -1.1146500722478976 2118 62-63 -1.4185195976891976 2118 64-65 0.23612118172751195 2118 66-67 -0.1462363388151502 2118 68-69 -0.011647635223269504 2118 70-71 0.36644730396027825 2118 72-73 3.334083132035289 2118 74-75 2.2169922315830703 2118 76 2.030813941059499 2119 1 0.16759154385724884 2119 2 -1.5473940446014396 2119 3 -0.5237524984667417 2119 4 -0.18428296871006467 2119 5 0.021530185677470115 2119 6 -0.9714233138718598 2119 7 -1.2249914521757361 2119 8 0.12488575744615105 2119 9 -0.25716377444746996 2119 10-11 -0.5247122355561089 2119 12-13 -0.8081815465473596 2119 14-15 -0.301615852502394 2119 16-17 -0.391970050631258 2119 18-19 -0.5017970866388097 2119 20-21 -2.214739224139059 2119 22-23 -0.9555237576043538 2119 24-25 0.31016485621213974 2119 26-27 -0.05052789140669489 2119 28-29 -1.3550567912490266 2119 30-31 0.2444290322705811 2119 32-33 -0.31717314428899357 2119 34-35 -0.45393831890229563 2119 36-37 -1.3908208249123106 2119 38-39 -1.5908395494031637 2119 40-41 -2.1458183820920596 2119 42-43 -0.7985615525666248 2119 44-45 -0.8778529692150769 2119 46-47 -0.653497960902854 2119 48-49 -1.2884621941628112 2119 50-51 0.46692173998221875 2119 52-53 -1.3545304039102106 2119 54-55 -0.26803950565990675 2119 56-57 -3.724827800522192 2119 58-59 -0.5913090010831326 2119 60-61 -0.817578230232229 2119 62-63 -1.999895150974865 2119 64-65 -1.4284458367720916 2119 66-67 -1.5550435116672645 2119 68-69 -0.5918882435537682 2119 70-71 -1.1310558171383533 2119 72-73 -0.35358001630201485 2119 74-75 -0.14681437379656614 2119 76 -0.12297110036598724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 1.0 22 5.0 23 16.0 24 39.0 25 93.0 26 170.0 27 343.0 28 486.0 29 830.0 30 1314.0 31 2012.0 32 2828.0 33 4027.0 34 5586.0 35 6935.0 36 9924.0 37 12815.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.795259983975036 23.421751781723106 10.939147303167038 30.84384093113482 2 17.823556698857168 18.32328258761017 42.97220933665078 20.880951376881878 3 16.14304389997048 27.731624003711048 34.66874710074643 21.456584995572047 4 11.80154345717539 22.717496731750515 36.03086914350778 29.450090667566315 5 11.403027875005272 38.71083371990048 36.184793151435926 13.701345253658332 6 22.78707881752625 37.75144435541686 17.366001771180365 22.095475055876523 7 22.662674482351452 35.77151773288913 19.664319149833425 21.90148863492599 8 19.70859865896344 34.810019820351705 23.894066545776578 21.587314974908278 9 19.79926622527727 19.757095264201073 19.47454982499051 40.96908868553114 10-11 20.494032809007717 24.743811411462065 30.46957365158352 24.292582127946698 12-13 23.321595749167123 19.94791886307089 27.83283431029393 28.897651077468055 14-15 22.996879348880363 23.92147767047611 21.665331252899254 31.416311727744272 16-17 24.082781596592586 27.65149917766626 21.671656897060686 26.594062328680472 18-19 21.767595833509045 26.891367604267703 22.629991987517396 28.71104457470586 20-21 21.337452030531775 24.917766625901404 22.580441108252856 31.164340235313965 22-23 23.628389490996497 29.892885758866445 22.510859022477124 23.967865727659934 24-25 23.781259224897735 31.551258803188126 22.349555096360646 22.317926875553493 26-27 27.789609075190825 28.725804411082528 22.993716526799645 20.490869986927002 28-29 27.927718972715386 23.62417239488888 23.56407877535529 24.884029857040442 30-31 24.327373170834562 22.80394720195673 26.415890018133513 26.45278960907519 32-33 24.93147218825117 25.92248977354194 27.59140555813267 21.554632480074222 34-35 23.402774849238813 28.093239994939484 26.757474802850755 21.746510352970944 36-37 19.4724412769367 30.72470796609455 27.73056972968414 22.072281027284614 38-39 20.732298739088264 24.91038670771307 28.21975287816809 26.137561675030575 40-41 19.784506388900603 24.947286298654745 30.979842280605574 24.288365031839078 42-43 24.080673048538774 24.171340614852614 31.67250031628221 20.0754860203264 44-45 19.967950069582084 25.071690633829547 29.60296040146755 25.35739889512082 46-47 24.288365031839078 22.00375321553578 28.114325475477585 25.593556277147556 48-49 22.139754565006537 21.688525281491163 29.50807573904609 26.663644414456204 50-51 17.88997596255219 21.67692826719521 33.4363007632944 26.99679500695821 52-53 19.595791338084595 21.8624804959305 36.301817568422386 22.239910597562517 54-55 17.880487496310042 22.021675873993168 33.571247838738245 26.526588790958545 56-57 22.367477754818033 21.790789862100958 30.79534432589719 25.046388057183822 58-59 18.43081853835449 28.176527643064986 32.103698393286386 21.28895542529414 60-61 19.54940328090077 34.6213047695357 25.41749251465441 20.411799434909124 62-63 15.859444186733015 39.66495171424957 23.600978366296967 20.874625732720446 64-65 15.930080546535654 40.3786952304643 22.7143339096698 20.97689031333024 66-67 16.007042550499726 39.31598701134399 22.1323746468182 22.544595791338086 68-69 15.61485261249104 37.607008813730864 22.645806097920975 24.132332475857122 70-71 16.360224349512926 35.40673891957998 23.137097794458736 25.095938936448363 72-73 17.384978703664654 32.86172141863113 22.704845443427658 27.048454434276557 74-75 17.907898621009576 32.36726690001265 22.57938683422595 27.145447644751826 76 18.253700501834437 33.038839455151184 22.18614262219036 26.52131742082402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 1.5 16 3.0 17 3.0 18 5.0 19 10.0 20 14.0 21 15.0 22 15.5 23 30.5 24 51.5 25 58.0 26 80.5 27 130.0 28 176.0 29 195.0 30 254.0 31 385.0 32 549.0 33 641.0 34 719.5 35 924.5 36 1164.0 37 1277.0 38 1412.0 39 1734.5 40 2171.0 41 2670.5 42 2921.0 43 3477.5 44 4560.0 45 5038.5 46 4991.0 47 4676.0 48 3887.0 49 3068.0 50 2723.0 51 2438.5 52 1885.5 53 1514.5 54 1412.0 55 1288.0 56 1009.0 57 733.0 58 612.0 59 501.0 60 327.0 61 223.5 62 183.0 63 139.0 64 75.5 65 44.0 66 24.5 67 17.0 68 10.5 69 2.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 47426.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.633576519208866 #Duplication Level Percentage of deduplicated Percentage of total 1 70.65139011297171 37.18635347699574 2 15.43546190209118 16.248471302660988 3 6.073231311593622 9.589676548728546 4 2.9845364954731193 6.283473200354236 5 1.6064417915231153 4.227638847889343 6 1.065619742007852 3.365242693880994 7 0.6930534412306707 2.5534516931640874 8 0.5167855139812515 2.1760215915320713 9 0.2724140693854659 1.2904314089318096 >10 0.63696819165131 5.014127271960528 >50 0.03605480330101755 1.3557963985999242 >100 0.016024357022674466 1.5033947623666344 >500 0.0040060892556686165 1.5603255598195083 >1k 0.008012178511337233 7.645595243115591 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT 1941 4.092691772445494 TruSeq Adapter, Index 8 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT 1685 3.5529034706700964 No Hit CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 740 1.5603255598195083 No Hit CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 311 0.6557584447349555 No Hit TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT 159 0.33525914055581324 No Hit TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 123 0.259351410618648 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT 120 0.2530257664572176 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT 94 0.19820351705815376 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 88 0.1855522287352929 No Hit CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 85 0.17922658457386245 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT 75 0.15814110403576098 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGT 67 0.14127271960527982 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT 63 0.13283852739003923 No Hit CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 59 0.12440433517479864 No Hit CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 57 0.12018723906717835 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 55 0.11597014295955804 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 50 0.10542740269050732 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010542740269050731 0.0 2 0.0 0.0 0.0 0.010542740269050731 0.0 3 0.0 0.0 0.0 0.010542740269050731 0.0 4 0.0 0.0 0.0 0.012651288322860879 0.0 5 0.0 0.0 0.0 0.012651288322860879 0.0 6 0.0 0.0 0.0 0.012651288322860879 0.0 7 0.0 0.0 0.0 0.012651288322860879 0.0 8 0.0 0.0 0.0 0.012651288322860879 0.0 9 0.0 0.0 0.0 0.018976932484291317 0.0 10 0.0 0.0 0.0 0.021085480538101463 0.0 11 0.0 0.0 0.0 0.021085480538101463 0.0 12 0.0 0.0 0.0 0.021085480538101463 0.0 13 0.0 0.0 0.0 0.021085480538101463 0.0 14 0.0 0.0 0.0 0.02319402859191161 0.0 15 0.0 0.0 0.0 0.02319402859191161 0.0 16 0.0 0.0 0.0 0.02319402859191161 0.0 17 0.0 0.0 0.0 0.025302576645721758 0.0 18 0.0 0.0 0.0 0.0316282208071522 0.0 19 0.0 0.0 0.0 0.042170961076202926 0.0 20 0.0 0.0 0.0 0.04427950913001307 0.0 21 0.0 0.0 0.0 0.050605153291443515 0.0 22 0.0 0.0 0.0 0.05271370134525366 0.0 23 0.0 0.0 0.0 0.0590393455066841 0.0 24 0.0 0.0 0.0 0.0590393455066841 0.0 25 0.0 0.0 0.0 0.0590393455066841 0.0 26 0.0 0.0 0.0 0.06114789356049424 0.0 27 0.0 0.0 0.0 0.08434192215240585 0.0 28 0.0 0.0 0.0 0.10121030658288703 0.0 29 0.0 0.0 0.0 0.1180786910133682 0.0 30 0.0 0.0 0.0 0.1454898157129001 0.0 31 0.0 0.0 0.0 0.17500948846624215 0.0 32 0.0 0.0 0.0 0.21085480538101464 0.0 33 0.0 0.0 0.0 0.2530257664572176 0.0 34 0.0 0.0 0.0 0.30573946780247124 0.0 35 0.0 0.0 0.0 0.3647788133091553 0.0 36 0.0 0.0 0.0 0.4723147640534728 0.0 37 0.0 0.0 0.0 0.6473242525197149 0.0 38 0.0 0.0 0.0 0.9383038839455151 0.0 39 0.0 0.0 0.0 1.3431451102770633 0.0 40 0.0 0.0 0.0 1.7669632690929027 0.0 41 0.0 0.0 0.0 2.3257285033525914 0.0 42 0.0 0.0 0.0 3.013115168894699 0.0 43 0.0 0.0 0.0 3.7489984396744402 0.0 44 0.0 0.0 0.0 4.404756884409395 0.0 45 0.0 0.0 0.0 5.100577742166744 0.0 46 0.0 0.0 0.0 5.745793446632649 0.0 47 0.0 0.0 0.0 6.399443343313794 0.0 48 0.0 0.0 0.0 6.981402606165394 0.0 49 0.0 0.0 0.0 7.632943954792729 0.0 50 0.0 0.0 0.0 8.185383544890987 0.0 51 0.0 0.0 0.0 8.900181335132627 0.0 52 0.0 0.0 0.0 9.733057816387635 0.0 53 0.0 0.0 0.0 10.565934297642643 0.0 54 0.0 0.0 0.0 11.36507401003669 0.0 55 0.0 0.0 0.0 12.075654704170708 0.0 56 0.0 0.0 0.0 12.803103782735208 0.0 57 0.0 0.0 0.0 13.355543372833466 0.0 58 0.0 0.0 0.0 13.920634251254587 0.0 59 0.0 0.0 0.0 14.473073841352845 0.0 60 0.0 0.0 0.0 15.12250664192637 0.0 61 0.0 0.0 0.0 15.674946232024627 0.0 62 0.0 0.0 0.0 16.288533715683382 0.0 63 0.0 0.0 0.0 16.876818622696412 0.0 64 0.0 0.0 0.0 17.513600134947076 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGCCG 15 0.0022117316 70.00001 40 AGCAAGG 15 0.0022117316 70.00001 5 CGTCTGC 15 0.0022117316 70.00001 38 CTGACCT 15 0.0022117316 70.00001 25 TAGCAAG 15 0.0022117316 70.00001 4 TACTGAC 15 0.0022117316 70.00001 3 CTGCCGT 15 0.0022117316 70.00001 41 TTGGTAA 15 0.0022117316 70.00001 29 GAACAAA 15 0.0022117316 70.00001 59 CTTAATA 25 2.3676449E-4 55.999996 1 TTAATAC 25 2.3676449E-4 55.999996 2 TGGTCTA 25 2.3676449E-4 55.999996 43 ACGCGAC 20 0.0069111926 52.5 22 GCGACGG 20 0.0069111926 52.5 24 CTCGTCT 20 0.0069111926 52.5 36 TAAAGTG 20 0.0069111926 52.5 68 GTCTGCC 20 0.0069111926 52.5 39 CGACGGC 20 0.0069111926 52.5 25 CGCGACG 20 0.0069111926 52.5 23 ACAGGCC 20 0.0069111926 52.5 8 >>END_MODULE