FastQCFastQC Report
Wed 25 May 2016
SRR1780401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539612
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGT357.243041E-637.74453740
CTCGGTT250.002358461335.17922641
AAAGGTC300.005717167629.35958916
AAGCAAC300.005719763729.35686334
GACGCGA406.952506E-427.55020720
ATCGTGG508.287673E-526.42117534
AATGACG450.001392202324.45950922
CGTTTAC551.5843818E-424.01702114
CGGTGGT1850.023.76754243
GCCGGAA651.8140498E-523.68711328
AGAGATC651.8310555E-523.6585757
TCGGTGG2800.023.55751642
TTTACAC2650.023.2100372
CGACAAT952.652996E-823.17862317
CTTTATA952.7217538E-823.1227822
CGCCGAG1254.0017767E-1122.9068619
TGACGTC500.002563852822.02173624
GTTCCAC500.002566620222.01764537
GATGCAA500.002566620222.01764535
AGATGCA500.002566620222.01764534
Produced by FastQC (version 0.11.5)