FastQCFastQC Report
Wed 25 May 2016
SRR1780401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539612
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT306135.673150337650015No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT295725.480233945872219RNA PCR Primer, Index 17 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC99421.8424349347308806No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC46640.8643247370332757No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG32260.5978369643373387No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT23240.4306798217978844RNA PCR Primer, Index 17 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG16360.30318080398508557No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC14940.27686559972721136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC14920.276494963047523No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG10480.19421362015670518No Hit
CTTAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC9320.1727166927347798No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC9300.17234605605509143No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC6650.12323669599638258No Hit
CTTATTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5910.10952313884791294No Hit
CTTATCACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC5760.10674336375025018No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC5660.10489018035180833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT30650.041.7701344
CTATACA6350.039.188731
GCCGTCT33200.038.4956243
TTTACAC10950.036.762942
CTTTACA11350.036.0888941
CTTATAA1700.034.9760931
GAGCCAT1050.033.53553414
TTACACA12450.032.5103573
AACCGTA353.2176645E-431.4257
CTTTATA2450.030.5271422
TTATACA47400.030.3045672
CTTATAC48050.030.1570571
TCGTGGA951.8189894E-1230.1102099
CCTCGTG951.8189894E-1230.101847
CTGATCG300.005740221629.33543216
GGCGCAC300.005742822329.33271422
GATCGGC300.00574542429.32999618
CTGCGAG300.00574542429.32999628
TTAATAC2600.027.0738432
ATTGGCG1006.002665E-1126.39944321
Produced by FastQC (version 0.11.5)