FastQCFastQC Report
Wed 25 May 2016
SRR1780400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55893
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCCG150.002214481169.994636
TAAGGAT150.002214481169.9946352
GGTACTA200.00691972852.4959739
TGCTTGG200.00691972852.495977
TGCGGTA200.00691972852.4959736
AGAGTGG200.00691972852.495975
GAAACCG200.00691972852.4959760
CCTGGAT200.00691972852.4959768
CGGTACT200.00691972852.4959738
CCTTGCG200.00691972852.4959733
ACGAACT200.00691972852.495972
CGACGAA350.001246010239.99693321
CGTATTA400.002400748734.99731415
AGTTTTT450.00427539631.10872722
CAGCAAA450.00427539631.10872720
AGGCCTT450.00427539631.10872710
TTTACAC500.007155378327.9978522
TTACACA755.497718E-527.997853
ACCCTAC1003.7327645E-421.1117921
CATCTTC1003.8713968E-420.99838814
Produced by FastQC (version 0.11.5)