##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780400_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55893 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.304778773728373 34.0 27.0 34.0 12.0 34.0 2 30.26633030969889 34.0 32.0 34.0 12.0 34.0 3 30.317785769237652 34.0 32.0 34.0 11.0 34.0 4 30.19549854185676 34.0 32.0 34.0 11.0 34.0 5 30.475408369563272 34.0 32.0 34.0 11.0 34.0 6 33.31703433345857 38.0 34.0 38.0 11.0 38.0 7 32.802587086039395 38.0 34.0 38.0 11.0 38.0 8 31.932191866602256 37.0 31.0 38.0 11.0 38.0 9 32.833914801495716 37.0 33.0 38.0 12.0 38.0 10-11 32.80950208433972 37.5 34.0 38.0 11.0 38.0 12-13 32.9301254182098 38.0 34.0 38.0 11.0 38.0 14-15 32.74506646628379 38.0 33.5 38.0 11.0 38.0 16-17 32.57952695328574 38.0 32.0 38.0 11.0 38.0 18-19 32.6436763100925 38.0 32.5 38.0 11.0 38.0 20-21 32.69422825756356 38.0 32.5 38.0 11.0 38.0 22-23 32.51849068756374 37.5 32.0 38.0 11.0 38.0 24-25 32.87073515467053 38.0 34.0 38.0 11.0 38.0 26-27 32.77809385790707 38.0 34.0 38.0 11.0 38.0 28-29 32.829406186821245 38.0 34.0 38.0 11.0 38.0 30-31 32.79314941048074 38.0 34.0 38.0 11.0 38.0 32-33 32.41266348201027 37.5 32.0 38.0 11.0 38.0 34-35 32.66382194550302 37.5 33.5 38.0 11.0 38.0 36-37 32.633505090082835 38.0 33.5 38.0 11.0 38.0 38-39 32.840445136242465 38.0 34.0 38.0 11.0 38.0 40-41 32.57768414649419 37.5 33.0 38.0 11.0 38.0 42-43 32.74861789490634 38.0 34.0 38.0 11.0 38.0 44-45 31.990642835417674 37.0 29.0 38.0 11.0 38.0 46-47 32.63289678492835 37.0 33.5 38.0 11.0 38.0 48-49 32.57754996153365 37.0 32.5 38.0 11.0 38.0 50-51 32.4002200633353 37.0 31.5 38.0 11.0 38.0 52-53 32.40921940135617 37.0 31.5 38.0 11.0 38.0 54-55 32.2321041990947 37.0 31.0 38.0 11.0 38.0 56-57 32.16127243125257 37.0 31.0 38.0 11.0 38.0 58-59 32.16253376988174 37.0 31.0 38.0 11.0 38.0 60-61 32.16853631045032 37.0 31.0 38.0 11.0 38.0 62-63 32.14834594671963 37.0 31.0 38.0 11.0 38.0 64-65 32.24369777968619 37.0 31.0 38.0 11.0 38.0 66-67 32.21106399728052 37.0 31.0 38.0 11.0 38.0 68-69 31.583588284758378 37.0 29.0 38.0 11.0 38.0 70-71 31.8443365000984 37.0 30.5 38.0 11.0 38.0 72-73 32.00699550927665 37.0 30.5 38.0 11.0 38.0 74-75 31.563693127940887 37.0 28.5 38.0 11.0 38.0 76 30.97110550516165 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.597856594239257 1101 2 -0.10194990559394768 1101 3 0.8919240074082673 1101 4 0.12613048784789527 1101 5 0.02646252363604873 1101 6 -0.401074458926999 1101 7 1.0571414047862149 1101 8 0.6165955788404531 1101 9 0.22247960920577725 1101 10-11 -0.17865738864582426 1101 12-13 0.182246450680978 1101 14-15 0.1306718131599709 1101 16-17 0.5228042142137497 1101 18-19 1.169406355111434 1101 20-21 -0.13112705775968436 1101 22-23 1.0251295990799534 1101 24-25 -0.1697855011896081 1101 26-27 0.579521765522756 1101 28-29 0.6123824249621137 1101 30-31 0.5931010198947817 1101 32-33 0.5320652979720961 1101 34-35 0.23445178095609975 1101 36-37 0.5500906152581919 1101 38-39 0.11848266839751176 1101 40-41 0.7117388549154597 1101 42-43 0.8371059643705294 1101 44-45 0.802820061933037 1101 46-47 0.7252596182612123 1101 48-49 -0.05766733839836746 1101 50-51 0.06585667333958867 1101 52-53 1.010968598675312 1101 54-55 0.12200060716843097 1101 56-57 0.44167341096678214 1101 58-59 0.8054341567599401 1101 60-61 0.6196645052705847 1101 62-63 -0.26579737514724044 1101 64-65 0.3067331862850331 1101 66-67 0.5796388346829637 1101 68-69 1.3779141798410706 1101 70-71 1.347646414659451 1101 72-73 0.9417505374951496 1101 74-75 -0.10226993317727917 1101 76 -1.0694613791276915 1102 1 1.2044689871560905 1102 2 0.19322935797194418 1102 3 0.8324521081834604 1102 4 0.7866198289331692 1102 5 0.47268345386860844 1102 6 0.4165127503753254 1102 7 0.785096831142809 1102 8 1.1001227106233955 1102 9 1.3308856169577155 1102 10-11 0.5824860222068935 1102 12-13 -1.5454667276135936 1102 14-15 -0.10340037676251512 1102 16-17 -1.236704022220362 1102 18-19 -0.397635796051361 1102 20-21 0.6703506554186163 1102 22-23 0.315342777374525 1102 24-25 0.963935429042948 1102 26-27 -3.882990346880348 1102 28-29 0.33264647534971203 1102 30-31 0.5693607098172677 1102 32-33 -6.340148849314723 1102 34-35 0.07971583134369098 1102 36-37 0.9884384834752424 1102 38-39 0.917659024986655 1102 40-41 0.7084685060782547 1102 42-43 0.6046083868511545 1102 44-45 -4.782451255896422 1102 46-47 -0.26299135073102775 1102 48-49 0.10021783602024215 1102 50-51 -4.252336156117778 1102 52-53 -0.42701589744872237 1102 54-55 1.3085316149203763 1102 56-57 -0.741102751823913 1102 58-59 1.0203687497831986 1102 60-61 0.8759023928674878 1102 62-63 -3.4519044487906463 1102 64-65 -0.21010305402504414 1102 66-67 0.4748059858457587 1102 68-69 -6.414842603104667 1102 70-71 0.2145916665974319 1102 72-73 0.5353794134641454 1102 74-75 -4.985021871161777 1102 76 -1.2391561465695524 1103 1 0.1502136680071544 1103 2 -1.0873779115316005 1103 3 -0.7768785655754051 1103 4 0.34555156652182006 1103 5 0.08890685812392718 1103 6 -1.1841521432416968 1103 7 0.47742839142649274 1103 8 1.1510092354460895 1103 9 -0.06554612063094112 1103 10-11 0.6416172278806442 1103 12-13 -0.9841192098830973 1103 14-15 -0.7382297207341395 1103 16-17 -0.9163471959792275 1103 18-19 -0.6491982960513596 1103 20-21 -0.5720473942267787 1103 22-23 0.6760697277291356 1103 24-25 0.3804247907450744 1103 26-27 -0.44250940716403164 1103 28-29 0.09553432995964073 1103 30-31 0.3029600005974018 1103 32-33 -4.581616047896283 1103 34-35 -0.41909844170595534 1103 36-37 -0.08379112645383202 1103 38-39 -0.14612643600624864 1103 40-41 -0.1148692598791925 1103 42-43 0.06523560316320243 1103 44-45 -4.609401610506357 1103 46-47 -1.125779471298408 1103 48-49 -0.501012217171251 1103 50-51 -0.2425178937064345 1103 52-53 -0.6920868194345395 1103 54-55 -0.4644293779874289 1103 56-57 0.0801073545590647 1103 58-59 0.2539126327619208 1103 60-61 0.32901631130719977 1103 62-63 0.0374683348972944 1103 64-65 0.265849960159354 1103 66-67 -0.581011833303176 1103 68-69 -2.9043594470763026 1103 70-71 0.17085274815771712 1103 72-73 0.14750264041449768 1103 74-75 -2.285199176126312 1103 76 -0.760233274229126 1104 1 -1.5342187956493376 1104 2 0.08154478029472045 1104 3 0.2694100644428872 1104 4 0.44284626181401165 1104 5 0.37172191540706834 1104 6 0.5314731576151459 1104 7 0.3961922308411445 1104 8 1.2677320620562753 1104 9 0.9339917632623909 1104 10-11 0.3770750116488131 1104 12-13 0.18341713362320888 1104 14-15 0.16921227406704986 1104 16-17 0.20007718064540114 1104 18-19 1.053969124763114 1104 20-21 1.2623802557202737 1104 22-23 0.7145697758662308 1104 24-25 0.894365293296346 1104 26-27 1.0799032240095485 1104 28-29 0.9272566751988833 1104 30-31 -0.1722493957634299 1104 32-33 0.1185379908059474 1104 34-35 0.5069052167132284 1104 36-37 1.025085353761824 1104 38-39 0.7716842889383955 1104 40-41 0.988375180286404 1104 42-43 0.8837491296867483 1104 44-45 -2.360128480632472 1104 46-47 0.33262045168223864 1104 48-49 0.7782792047985225 1104 50-51 1.1846218001416489 1104 52-53 0.6399147209524827 1104 54-55 0.07615982004858068 1104 56-57 1.1102122934249543 1104 58-59 1.1869057972945107 1104 60-61 0.23599619000172112 1104 62-63 1.1275520934416257 1104 64-65 0.921236501027753 1104 66-67 0.45356022792720907 1104 68-69 -3.6199943753520287 1104 70-71 -0.27142124818386293 1104 72-73 0.6011081087884271 1104 74-75 0.08982729927563327 1104 76 0.5236407991318046 1105 1 -0.6020527519743446 1105 2 0.21003189420382995 1105 3 0.37316768789360566 1105 4 0.21030642313606762 1105 5 0.17105301908599913 1105 6 0.6347192721144523 1105 7 0.6194265412877371 1105 8 0.6432386526523857 1105 9 1.0131772836243798 1105 10-11 1.0620150077141375 1105 12-13 0.2146781999226377 1105 14-15 0.7085787899041591 1105 16-17 0.5271773183593496 1105 18-19 0.8681930082964655 1105 20-21 0.7478415974475965 1105 22-23 1.137263067229604 1105 24-25 0.7932832551299072 1105 26-27 0.5676663560182007 1105 28-29 0.5106129608569532 1105 30-31 0.7619331735853763 1105 32-33 0.5501138318446976 1105 34-35 0.7750736211987643 1105 36-37 0.9723605849245232 1105 38-39 0.5916626481750669 1105 40-41 0.45969133216521385 1105 42-43 0.6909987854018738 1105 44-45 1.714650193378933 1105 46-47 1.0206590115878171 1105 48-49 1.0504216403680644 1105 50-51 0.5905533366358426 1105 52-53 0.24870874023243772 1105 54-55 -0.1883886749346999 1105 56-57 0.8359352916543479 1105 58-59 0.564458423696248 1105 60-61 0.49802648707037633 1105 62-63 1.2573573265716682 1105 64-65 0.7853226706126328 1105 66-67 0.9080260945414089 1105 68-69 1.2533186287793896 1105 70-71 1.5431469202206216 1105 72-73 0.8286312250583485 1105 74-75 0.35374624478025396 1105 76 0.19712102734349202 1106 1 0.1058203385074421 1106 2 0.4966358895034766 1106 3 0.0182910721474272 1106 4 0.12358491902325852 1106 5 0.5534266971118527 1106 6 -0.4738588712462928 1106 7 1.064432416728394 1106 8 1.0219695574702428 1106 9 1.2645286349757328 1106 10-11 0.3789950312158936 1106 12-13 0.6495783174314482 1106 14-15 0.11938960071496751 1106 16-17 0.895038657959816 1106 18-19 0.6426935958405267 1106 20-21 0.7184643941573512 1106 22-23 0.9855004350321863 1106 24-25 1.410557050664572 1106 26-27 0.973189270236773 1106 28-29 0.25056595733169473 1106 30-31 0.7631670161235675 1106 32-33 1.2236743488834776 1106 34-35 1.227365043055407 1106 36-37 1.038635555547323 1106 38-39 0.8723324484100843 1106 40-41 0.6985867493214997 1106 42-43 0.9639130039682655 1106 44-45 1.7436748702297002 1106 46-47 0.5191313970167144 1106 48-49 0.17627357926348708 1106 50-51 0.3497190240624022 1106 52-53 0.49077689534406943 1106 54-55 0.25931990320866305 1106 56-57 0.9363465725004154 1106 58-59 0.38772516870211504 1106 60-61 1.0680617397143237 1106 62-63 1.038790934092237 1106 64-65 0.8457809549839688 1106 66-67 1.109729972332243 1106 68-69 2.180286901399839 1106 70-71 0.7291046170478808 1106 72-73 0.6291012603109856 1106 74-75 -0.11452637566627999 1106 76 -0.45799060602901065 1107 1 1.220524033027651 1107 2 1.1969086393175097 1107 3 1.3659479032904969 1107 4 1.033800639330721 1107 5 1.2037614355199828 1107 6 1.5028086219349603 1107 7 2.1005593693691083 1107 8 0.8313918237732452 1107 9 0.6232690267436425 1107 10-11 1.9748789885677454 1107 12-13 1.5179889298787543 1107 14-15 0.5980283311885657 1107 16-17 1.1599655114187826 1107 18-19 1.3127283094532274 1107 20-21 1.7644351355409356 1107 22-23 1.3075063859372236 1107 24-25 0.9329721262906503 1107 26-27 1.1728917249850994 1107 28-29 0.5202659325362546 1107 30-31 0.8768912908570172 1107 32-33 1.7175250803488922 1107 34-35 1.4622894169705987 1107 36-37 1.3410283152795301 1107 38-39 1.6614472512863543 1107 40-41 1.0420407537846756 1107 42-43 1.2695787614688925 1107 44-45 1.466784218109698 1107 46-47 1.3215365544677482 1107 48-49 1.1137642809743724 1107 50-51 1.242168813301177 1107 52-53 1.5106752340497493 1107 54-55 1.3771478228714429 1107 56-57 1.4872516059742544 1107 58-59 1.2146778094162265 1107 60-61 0.833447308769621 1107 62-63 1.0436756353072134 1107 64-65 1.1402887655468241 1107 66-67 1.7287480730017215 1107 68-69 2.1103389718188765 1107 70-71 1.3366052369338206 1107 72-73 1.5761382055130717 1107 74-75 1.4136784346486237 1107 76 0.6721110552653116 1108 1 -0.02990601284390948 1108 2 0.3703126913052799 1108 3 0.48349377485012823 1108 4 0.7241198289331692 1108 5 0.5768501205352763 1108 6 1.4894294170419897 1108 7 2.113221831142809 1108 8 1.563664377290067 1108 9 1.086093950291044 1108 10-11 1.0746735222068935 1108 12-13 1.6133874390530707 1108 14-15 1.3158704565708277 1108 16-17 1.4638168111129772 1108 18-19 0.9252808706153104 1108 20-21 1.5687881554186092 1108 22-23 1.4533636107078607 1108 24-25 1.0524770957096123 1108 26-27 0.7003429864529878 1108 28-29 1.1894173086830406 1108 30-31 1.228214876483932 1108 32-33 1.5348511506852844 1108 34-35 1.048465831343698 1108 36-37 1.1030218168085852 1108 38-39 1.3030756916533264 1108 40-41 1.4766976727449261 1108 42-43 1.6462750535178117 1108 44-45 1.6159862441035706 1108 46-47 1.3385711492689722 1108 48-49 1.0403220026869064 1108 50-51 1.6174555105488864 1108 52-53 1.0990257692179455 1108 54-55 0.884052448253712 1108 56-57 1.680772248176087 1108 58-59 0.10630624978319858 1108 60-61 1.7821523928674807 1108 62-63 1.597574717876018 1108 64-65 1.3628136126416202 1108 66-67 0.6727226525124301 1108 68-69 2.051303230228669 1108 70-71 1.852612499930764 1108 72-73 1.7411085801308026 1108 74-75 2.21029062883823 1108 76 1.4587605200971119 1109 1 -0.00829697029071852 1109 2 -0.5320587625954296 1109 3 -0.3609211187668926 1109 4 -0.5140229015632869 1109 5 -0.5419442057058603 1109 6 -0.17457767515659128 1109 7 -0.8055503319777593 1109 8 -0.35111842412837646 1109 9 0.12487941128395619 1109 10-11 -0.16263809126829187 1109 12-13 -0.301672401372457 1109 14-15 -0.09248503988307988 1109 16-17 -0.7663471959792254 1109 18-19 -0.4140919130726388 1109 20-21 -0.019919734652312115 1109 22-23 -0.3420153786538407 1109 24-25 -0.13446882627620482 1109 26-27 -0.03187110929169279 1109 28-29 -0.33584864876376486 1109 30-31 -0.34863574408344533 1109 32-33 0.5593413989122311 1109 34-35 -0.4611197183016955 1109 36-37 -0.2587911264538292 1109 38-39 -0.1477221806870972 1109 40-41 0.04257754863144925 1109 42-43 -0.19221120534743363 1109 44-45 0.4666622192808809 1109 46-47 -0.5880135138515996 1109 48-49 -0.5435654086606121 1109 50-51 -0.17177321285537062 1109 52-53 0.20153020184205417 1109 54-55 -0.6378336333065775 1109 56-57 -0.5034032837388054 1109 58-59 -0.7487469417061625 1109 60-61 0.2476333325837956 1109 62-63 -0.5673188991452598 1109 64-65 -0.5267032313300071 1109 66-67 -0.09484162053722045 1109 68-69 0.40574693590242106 1109 70-71 -0.20467916673590025 1109 72-73 -0.42749735958550517 1109 74-75 -0.15966726123269837 1109 76 -0.3059779550801913 1110 1 -0.9100471418761664 1110 2 0.1672548956063551 1110 3 -0.4402965477305152 1110 4 -0.9234944183786595 1110 5 -0.3994939654862293 1110 6 0.03808533102048983 1110 7 0.3326102720030235 1110 8 0.2116818504083433 1110 9 -0.42875820024659106 1110 10-11 -1.2902290315565494 1110 12-13 -1.0158968351404774 1110 14-15 -0.5107760488055177 1110 16-17 -1.0087974361988543 1110 18-19 -0.6750047476642642 1110 20-21 -1.132102301570633 1110 22-23 -1.1614045344534318 1110 24-25 -0.5336452161183445 1110 26-27 -2.1105945135470137 1110 28-29 -1.3464697880911523 1110 30-31 -0.7775478923332741 1110 32-33 -0.6186098708200944 1110 34-35 -0.4800455933874872 1110 36-37 -1.0345117853419552 1110 38-39 -1.000553340604739 1110 40-41 -1.0059468165023908 1110 42-43 -0.3940643282026137 1110 44-45 0.1938928301250833 1110 46-47 -0.08130452277404032 1110 48-49 -0.6379374059152418 1110 50-51 -0.26056868299950153 1110 52-53 -0.53642449959926 1110 54-55 -1.342946879703284 1110 56-57 -0.7686229131142355 1110 58-59 -2.7892582663458363 1110 60-61 -0.1712582254120889 1110 62-63 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2116 62-63 1.6067210593394314 2116 64-65 1.3103491817473163 2116 66-67 0.9159264126750308 2116 68-69 1.1546441855132201 2116 70-71 -0.2656547764919992 2116 72-73 0.665587746797474 2116 74-75 0.8635553646105834 2116 76 1.3770786095280094 2117 1 -1.2919078244381126 2117 2 0.1042347927545535 2117 3 -1.513788833845524 2117 4 -1.9491138667190064 2117 5 -2.9014107490299423 2117 6 -0.9218024670159792 2117 7 -3.048689400741253 2117 8 -1.6915439560432688 2117 9 -2.240445904781417 2117 10-11 -1.7010284705467313 2117 12-13 -1.1867710754396832 2117 14-15 -1.8899719347335235 2117 16-17 -1.0952864497565926 2117 18-19 -1.676734527935416 2117 20-21 -1.4086801416828365 2117 22-23 -0.7562151936399673 2117 24-25 -1.751644281101978 2117 26-27 -0.19900031064846502 2117 28-29 -1.1285174739256547 2117 30-31 -1.0547334930812866 2117 32-33 -0.19046587830023043 2117 34-35 -0.8234771034389183 2117 36-37 -1.32474086435084 2117 38-39 -0.9931199605205876 2117 40-41 -0.860598522907253 2117 42-43 -0.9068273015546495 2117 44-45 -0.8331758935775859 2117 46-47 -0.6880366405861018 2117 48-49 -1.564071113255121 2117 50-51 0.05359681489671431 2117 52-53 -1.331001404695101 2117 54-55 -0.4695480952245532 2117 56-57 0.0815874655673916 2117 58-59 -0.9688749096370906 2117 60-61 -0.5483503245238204 2117 62-63 -1.5093093400949975 2117 64-65 -1.6849671844598255 2117 66-67 -1.028930427197718 2117 68-69 -0.4662465886119165 2117 70-71 -0.2590269928228537 2117 72-73 -0.0771658763909322 2117 74-75 -0.2621957842052538 2117 76 -0.32316882772897415 2118 1 -1.1500086247842063 2118 2 -2.430915542525568 2118 3 -1.8999017475379318 2118 4 -3.0869031810170817 2118 5 -1.6868314715045258 2118 6 -1.1417894884306463 2118 7 -1.4246357559218694 2118 8 -2.542290224699986 2118 9 -1.889807791002486 2118 10-11 -2.889217957892612 2118 12-13 -2.0616358818424523 2118 14-15 -2.2153018071107695 2118 16-17 -3.181161422717871 2118 18-19 -2.4357654602304635 2118 20-21 -1.5511201157256664 2118 22-23 -2.262704486307065 2118 24-25 -2.657333227673469 2118 26-27 -2.353878095636567 2118 28-29 -1.647336422660242 2118 30-31 -1.9321940165508948 2118 32-33 -1.4352203667276555 2118 34-35 -1.3554909472632701 2118 36-37 -2.2294564170222664 2118 38-39 -3.1530810247645817 2118 40-41 -2.3864475386978654 2118 42-43 -1.5437513768304427 2118 44-45 -2.071008469826772 2118 46-47 -1.8171735646613811 2118 48-49 -1.6699547386066236 2118 50-51 -2.2533467282570854 2118 52-53 -2.461219877548224 2118 54-55 -2.290659927368182 2118 56-57 -2.03424640854033 2118 58-59 -1.5550743472317272 2118 60-61 -1.6887353558887384 2118 62-63 -1.6356342373478618 2118 64-65 -2.523924571437977 2118 66-67 -3.4513833798258844 2118 68-69 -2.424715115542483 2118 70-71 -2.5218433458403773 2118 72-73 -2.1739644920084977 2118 74-75 -1.5080318214105333 2118 76 -2.150769952539701 2119 1 -0.5640495313624285 2119 2 0.08732658019416917 2119 3 0.050623404479757994 2119 4 -1.0183570229186856 2119 5 -0.20092765724250228 2119 6 -1.292977990365415 2119 7 -2.2507827984868243 2119 8 0.36401159951228834 2119 9 -1.1905264200793226 2119 10-11 0.019986022206893495 2119 12-13 -0.4621333942802579 2119 14-15 -0.33401380268843894 2119 16-17 -1.0425489296277703 2119 18-19 -1.7399390367921015 2119 20-21 -1.516570640877685 2119 22-23 -0.9925275929958417 2119 24-25 -1.7536571635496472 2119 26-27 -1.1309648839173825 2119 28-29 -0.14623083946511173 2119 30-31 -1.0764726235160715 2119 32-33 0.7495502247593535 2119 34-35 0.36935703504739337 2119 36-37 -0.6741772572654945 2119 38-39 -0.8103502342725974 2119 40-41 0.02386202459677378 2119 42-43 -0.6254263353710741 2119 44-45 -2.689858663303834 2119 46-47 -1.408245980360661 2119 48-49 -0.2021548491649483 2119 50-51 -0.3582389338955565 2119 52-53 -1.2742381196709474 2119 54-55 -0.8511906073018487 2119 56-57 -0.047237011083172575 2119 58-59 -0.4423048613279157 2119 60-61 -0.3084147367621455 2119 62-63 -0.2866845413832415 2119 64-65 -0.16322805402504414 2119 66-67 0.7436138562161254 2119 68-69 -0.5612546401417049 2119 70-71 0.22124675919002357 2119 72-73 -1.4918196606099308 2119 74-75 0.177015165875261 2119 76 0.10517255713414997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 21.0 13 169.0 14 566.0 15 940.0 16 1263.0 17 1529.0 18 1632.0 19 1407.0 20 1238.0 21 827.0 22 671.0 23 436.0 24 405.0 25 383.0 26 415.0 27 446.0 28 525.0 29 620.0 30 848.0 31 1088.0 32 1450.0 33 2055.0 34 3274.0 35 5669.0 36 11125.0 37 16891.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.520937842745923 29.28600967330133 11.077548231655788 28.115504252296958 2 16.202386703164976 28.98216234591094 31.914551017122 22.900899933802084 3 14.958939402071817 26.504213407761256 27.62599967795609 30.910847512210832 4 13.59383107008033 21.618091710947702 34.60898502495841 30.179092194013563 5 10.496842180595065 39.50941978423058 32.80732828797881 17.186409747195533 6 21.727228812194728 41.658168285831856 17.687366933247457 18.92723596872596 7 21.737963609038697 33.28323761472814 23.224732971928503 21.754065804304652 8 19.55343245129086 36.187000161021956 21.315728266509222 22.943839121177962 9 20.4605227846063 20.657327393412412 20.800458017998675 38.08169180398261 10-11 20.63675236612814 27.61258118190113 27.18945127296799 24.561215179002737 12-13 20.476624979872255 25.387794535988405 26.175907537616517 27.95967294652282 14-15 19.59458250585941 26.663446227613473 23.29093088579965 30.451040380727463 16-17 20.478414112679584 28.657434741380854 25.007603814431146 25.856547331508416 18-19 19.328001717567496 27.95967294652282 25.50945556688673 27.202869769022954 20-21 18.654393215608394 28.436476839675812 25.484407707584133 27.42472223713166 22-23 20.82461131089761 27.955200114504496 25.14894530621008 26.07124326838781 24-25 19.639310826042617 29.541266348201027 25.074696294705955 25.7447265310504 26-27 18.580144204104272 29.946504929060886 26.611561376200953 24.86178949063389 28-29 20.03292004365484 28.167212352172903 27.71008891990052 24.089778684271735 30-31 19.701930474299107 28.090279641457784 27.564274596103267 24.643515288139838 32-33 18.244681802730216 30.002862612491725 26.577567852861716 25.17488773191634 34-35 19.491707369438032 28.90701876800315 26.53999606390782 25.061277798650995 36-37 20.019501547599877 28.039289356448926 26.73143327429195 25.209775821659242 38-39 19.470237775750093 27.622421412341435 27.95967294652282 24.947667865385647 40-41 19.094519886211152 27.335265596765247 27.540121303204334 26.03009321381926 42-43 18.1453849319235 27.619737713130444 28.216413504374433 26.018463850571628 44-45 18.14180666630884 28.43826597248314 27.966829477752132 25.45309788345589 46-47 18.734009625534505 28.101014438301753 27.684146494194263 25.480829441969476 48-49 18.302828618968388 28.80772189719643 27.006065160216842 25.88338432361834 50-51 18.347556939151595 28.475837761437035 26.576673286458053 26.59993201295332 52-53 18.233052439482584 27.82459341956953 27.452453795645248 26.48990034530263 54-55 17.725833288605013 28.83813715492101 26.5006351421466 26.935394414327373 56-57 18.514840856636788 28.188681945860843 26.355715384753008 26.940761812749358 58-59 18.495160395756177 28.048235020485567 26.417440466605836 27.039164117152414 60-61 18.188324119299377 27.329898198343262 26.538206931100493 27.943570751256864 62-63 18.258994865188843 28.108170969531066 26.149070545506593 27.483763619773494 64-65 18.756373785626106 28.568872667418105 25.45130875064856 27.223444796307227 66-67 18.58372246971893 29.182545220331708 24.831374232909308 27.402358077040056 68-69 18.329665611078312 29.249637700606517 25.1408942085771 27.27980247973807 70-71 19.034583937165657 29.78548297640134 24.686454475515717 26.493478610917286 72-73 18.68838673894763 28.802354498774445 24.24632780491296 28.26293095736497 74-75 18.561358309627323 28.715581557619025 24.833163365716636 27.889896767037015 76 18.630239922709464 27.86753260694541 25.5076664340794 27.99456103626572 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 2.0 12 3.0 13 3.0 14 3.5 15 6.5 16 9.5 17 10.0 18 14.0 19 16.5 20 19.0 21 23.0 22 35.0 23 59.5 24 81.5 25 91.0 26 141.5 27 245.5 28 333.5 29 368.0 30 418.5 31 573.0 32 849.5 33 1022.0 34 1142.5 35 1424.5 36 1735.0 37 1884.0 38 2103.0 39 2624.0 40 3196.0 41 3526.0 42 3586.0 43 3579.0 44 3882.5 45 4119.5 46 4046.0 47 3999.5 48 3811.5 49 3456.5 50 3243.0 51 3029.5 52 2521.0 53 2037.0 54 1848.0 55 1735.5 56 1425.5 57 1053.0 58 878.0 59 758.5 60 539.5 61 408.0 62 376.0 63 289.5 64 184.5 65 133.0 66 82.5 67 65.0 68 62.0 69 47.5 70 36.5 71 37.0 72 27.5 73 18.0 74 16.0 75 14.0 76 10.5 77 7.0 78 7.0 79 7.0 80 5.5 81 5.0 82 6.0 83 6.0 84 4.5 85 2.0 86 2.5 87 4.0 88 5.0 89 4.5 90 3.0 91 2.0 92 1.0 93 0.5 94 2.0 95 3.5 96 3.0 97 1.5 98 1.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4938006548226075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55893.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.19599949904281 #Duplication Level Percentage of deduplicated Percentage of total 1 79.93139407244786 52.112071279051044 2 9.226125137211854 12.030128996475408 3 4.2618002195389675 8.335569749342493 4 2.088364434687157 5.446120265507308 5 1.517563117453348 4.946952212262716 6 0.8754116355653128 3.424400193226343 7 0.5982436882546652 2.7302166639829673 8 0.40065861690450055 2.0897071189594403 9 0.216794731064764 1.2720734260104127 >10 0.8836443468715698 7.612760095181866 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.001789132807328288 0.0 9 0.0 0.0 0.0 0.001789132807328288 0.0 10 0.0 0.0 0.0 0.003578265614656576 0.0 11 0.0 0.0 0.0 0.003578265614656576 0.0 12 0.0 0.0 0.0 0.007156531229313152 0.0 13 0.0 0.0 0.0 0.007156531229313152 0.0 14 0.0 0.0 0.0 0.007156531229313152 0.0 15 0.0 0.0 0.0 0.007156531229313152 0.0 16 0.0 0.0 0.0 0.007156531229313152 0.0 17 0.0 0.0 0.0 0.007156531229313152 0.0 18 0.0 0.0 0.0 0.00894566403664144 0.0 19 0.0 0.0 0.0 0.021469593687939454 0.0 20 0.0 0.0 0.0 0.023258726495267744 0.0 21 0.0 0.0 0.0 0.02683699210992432 0.0 22 0.0 0.0 0.0 0.02683699210992432 0.0 23 0.0 0.0 0.0 0.02683699210992432 0.0 24 0.0 0.0 0.0 0.028626124917252607 0.0 25 0.0 0.0 0.0 0.030415257724580896 0.0 26 0.0 0.0 0.0 0.030415257724580896 0.0 27 0.0 0.0 0.0 0.03757178895389405 0.0 28 0.0 0.0 0.0 0.04115005456855062 0.0 29 0.0 0.0 0.0 0.0447283201832072 0.0 30 0.0 0.0 0.0 0.05725224983450521 0.0 31 0.0 0.0 0.0 0.06977617948580322 0.0 32 0.0 0.0 0.0 0.08051097632977296 0.0 33 0.0 0.0 0.0 0.08766750755908612 0.0 34 0.0 0.0 0.0 0.10913710124702557 0.0 35 0.0 0.0 0.0 0.13060669493496502 0.0 36 0.0 0.0 0.0 0.1789132807328288 0.0 37 0.0 0.0 0.0 0.2522677258332886 0.0 38 0.0 0.0 0.0 0.3399352333923747 0.0 39 0.0 0.0 0.0 0.4794875923639812 0.0 40 0.0 0.0 0.0 0.6422986778308554 0.0 41 0.0 0.0 0.0 0.8426815522516237 0.0 42 0.0 0.0 0.0 1.0842144812409424 0.0 43 0.0 0.0 0.0 1.3436387383035442 0.0 44 0.0 0.0 0.0 1.6406347843200402 0.0 45 0.0 0.0 0.0 1.900059041382642 0.0 46 0.0 0.0 0.0 2.200633353013794 0.0 47 0.0 0.0 0.0 2.490472867800977 0.0 48 0.0 0.0 0.0 2.7731558513588466 0.0 49 0.0 0.0 0.0 3.045104038072746 0.0 50 0.0 0.0 0.0 3.3385218184745855 0.0 51 0.0 0.0 0.0 3.6498309269497073 0.0 52 0.0 0.0 0.0 3.9307247777002488 0.0 53 0.0 0.0 0.0 4.227720823716744 0.0 54 0.0 0.0 0.0 4.506825541659958 0.0 55 0.0 0.0 0.0 4.846760775052332 0.0 56 0.0 0.0 0.0 5.122287227380888 0.0 57 0.0 0.0 0.0 5.4228615390120405 0.0 58 0.0 0.0 0.0 5.6983879913405975 0.0 59 0.0 0.0 0.0 5.982860107705795 0.0 60 0.0 0.0 0.0 6.247651763190381 0.0 61 0.0 0.0 0.0 6.533913012362908 0.0 62 0.0 0.0 0.0 6.850589519260015 0.0 63 0.0 0.0 0.0 7.120748573166586 0.0 64 0.0 0.0 0.0 7.399853291109799 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCCCG 15 0.0022144811 69.99463 6 TAAGGAT 15 0.0022144811 69.99463 52 GGTACTA 20 0.006919728 52.49597 39 TGCTTGG 20 0.006919728 52.49597 7 TGCGGTA 20 0.006919728 52.49597 36 AGAGTGG 20 0.006919728 52.49597 5 GAAACCG 20 0.006919728 52.49597 60 CCTGGAT 20 0.006919728 52.49597 68 CGGTACT 20 0.006919728 52.49597 38 CCTTGCG 20 0.006919728 52.49597 33 ACGAACT 20 0.006919728 52.49597 2 CGACGAA 35 0.0012460102 39.996933 21 CGTATTA 40 0.0024007487 34.997314 15 AGTTTTT 45 0.004275396 31.108727 22 CAGCAAA 45 0.004275396 31.108727 20 AGGCCTT 45 0.004275396 31.108727 10 TTTACAC 50 0.0071553783 27.997852 2 TTACACA 75 5.497718E-5 27.99785 3 ACCCTAC 100 3.7327645E-4 21.111792 1 CATCTTC 100 3.8713968E-4 20.998388 14 >>END_MODULE