FastQCFastQC Report
Wed 25 May 2016
SRR1780400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55893
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT28065.020306657363176No Hit
CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC12152.17379636090387No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT4960.8874098724348309No Hit
CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC3270.5850464279963502No Hit
TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT1940.34709176462168784No Hit
CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC1120.20038287442076824No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG900.1610219526595459No Hit
CTTATAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG830.1484980230082479No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGTCTT820.14670889020091962No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGTCTT640.11450449966901044No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGTCTT620.11092623405435387No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC580.1037697028250407No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTT150.002213807570.07
GGAATGA150.002213807570.040
CTATGCG150.002213807570.041
TTAATAC252.8057384E-670.02
GCTATGC150.002213807570.040
CGTCTGC150.002213807570.038
AAGAAGC150.002213807570.058
GTTGTCT150.002213807570.049
GAGGTGC150.002213807570.035
TTGCGGC150.002213807570.024
CTACAGC150.002213807570.065
CTTTAGG150.002213807570.034
TACAGCG150.002213807570.066
AGGTGCT150.002213807570.036
TTTACAC2150.066.744192
CTTTACA2350.059.574471
GGTTTAA252.3713263E-455.99999610
AGACTTC200.00691762952.511
GACAGTT200.00691762952.526
GTACATG200.00691762952.51
Produced by FastQC (version 0.11.5)