##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780400_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55893 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.618646342117977 34.0 32.0 34.0 24.0 34.0 2 32.639847565884814 34.0 34.0 34.0 31.0 34.0 3 33.086289875297446 34.0 34.0 34.0 32.0 34.0 4 33.218846724992396 34.0 34.0 34.0 32.0 34.0 5 33.23516361619523 34.0 34.0 34.0 33.0 34.0 6 36.80346376111499 38.0 37.0 38.0 34.0 38.0 7 36.4232909308858 38.0 37.0 38.0 34.0 38.0 8 36.23748948884476 38.0 37.0 38.0 34.0 38.0 9 36.0750004472832 38.0 37.0 38.0 32.0 38.0 10-11 35.640035424829584 37.5 36.5 38.0 31.0 38.0 12-13 35.539817150627094 37.5 36.0 38.0 31.0 38.0 14-15 35.718005832572956 38.0 36.0 38.0 31.0 38.0 16-17 35.92840785071476 38.0 36.0 38.0 32.0 38.0 18-19 35.9479004526506 38.0 36.0 38.0 32.0 38.0 20-21 35.69468448642942 38.0 36.0 38.0 31.0 38.0 22-23 35.59661317159573 37.5 35.5 38.0 31.5 38.0 24-25 34.709757930331165 37.0 34.5 38.0 27.5 38.0 26-27 35.04700946451255 37.0 35.0 38.0 29.0 38.0 28-29 34.82071994704167 37.0 35.0 38.0 27.0 38.0 30-31 34.403932513910505 37.0 34.0 38.0 26.0 38.0 32-33 34.86163741434527 37.0 34.5 38.0 27.0 38.0 34-35 34.61989873508311 37.0 34.0 38.0 27.0 38.0 36-37 34.80776662551661 37.0 34.5 38.0 27.0 38.0 38-39 34.68093500080511 37.0 34.5 38.0 26.5 38.0 40-41 34.084822786395435 37.0 34.0 38.0 25.0 38.0 42-43 34.66568264362264 37.0 34.5 38.0 26.5 38.0 44-45 34.951496609593335 37.0 35.5 38.0 28.5 38.0 46-47 35.139892294205 37.0 36.0 38.0 28.5 38.0 48-49 34.7384287835686 37.0 34.5 38.0 26.5 38.0 50-51 35.076306514232556 37.0 35.5 38.0 27.0 38.0 52-53 34.895586209364325 37.0 35.0 38.0 27.0 38.0 54-55 35.07101068112286 37.0 35.0 38.0 27.0 38.0 56-57 35.151020700266585 37.0 36.0 38.0 29.0 38.0 58-59 35.30748036426744 37.0 36.0 38.0 31.0 38.0 60-61 34.78849766518169 37.0 35.0 38.0 27.0 38.0 62-63 35.1617912797667 37.0 35.5 38.0 29.0 38.0 64-65 35.246721414130576 37.0 36.0 38.0 30.0 38.0 66-67 34.220251194246146 37.0 34.0 38.0 24.5 38.0 68-69 32.82320684164385 37.0 32.0 38.0 21.5 38.0 70-71 32.15706796915535 37.0 31.0 38.0 13.5 38.0 72-73 31.59071797899558 36.5 27.5 38.0 11.0 38.0 74-75 32.31452954752831 37.0 31.0 38.0 11.0 38.0 76 31.907805986438373 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.19964496847637037 1101 2 0.9634676688370476 1101 3 0.056038188106228404 1101 4 0.6442615810357211 1101 5 0.6910551631440285 1101 6 -1.4425095672662707 1101 7 0.22269222790665566 1101 8 1.080645257867097 1101 9 0.8442810422130478 1101 10-11 0.29081036443020025 1101 12-13 1.4906227777679888 1101 14-15 1.275274248877146 1101 16-17 0.31109417835538267 1101 18-19 -0.19822628823975208 1101 20-21 -0.7370877475934705 1101 22-23 0.8403559239452534 1101 24-25 -1.9527892257597728 1101 26-27 -0.6227649611361628 1101 28-29 0.9259608103557184 1101 30-31 1.7810997295542847 1101 32-33 0.39242158253704673 1101 34-35 0.796433027168149 1101 36-37 1.2243470414801791 1101 38-39 -0.3283814233377669 1101 40-41 1.797451702713495 1101 42-43 0.9411704285906453 1101 44-45 0.8346084464838697 1101 46-47 1.795003804991751 1101 48-49 -0.4612867661441129 1101 50-51 -1.1425974617367487 1101 52-53 -1.8507868193872028 1101 54-55 -1.1039484703838696 1101 56-57 -1.4311579256752864 1101 58-59 -1.3668207417789588 1101 60-61 -2.0131576575538617 1101 62-63 -1.028624337632877 1101 64-65 -1.6221724496937497 1101 66-67 -2.548250631449065 1101 68-69 -1.6750451620473186 1101 70-71 -0.6847527303497287 1101 72-73 -1.0178629927861458 1101 74-75 -0.8771967217219938 1101 76 0.1968177136658653 1102 1 -0.6337534956081541 1102 2 0.8069754207750321 1102 3 0.6385236532225065 1102 4 0.5524495655318447 1102 5 0.306607488725426 1102 6 0.3825873319585327 1102 7 0.05239184030975963 1102 8 0.8094485524407347 1102 9 1.113297515081264 1102 10-11 0.9116315853604391 1102 12-13 1.2886484560625675 1102 14-15 0.6276240550787051 1102 16-17 0.6568427248670119 1102 18-19 -0.2696894665343308 1102 20-21 0.9436898686856026 1102 22-23 0.659941679759207 1102 24-25 -3.953879342038846 1102 26-27 -0.21312348826793936 1102 28-29 0.9150596475650232 1102 30-31 2.0903899427325854 1102 32-33 0.5426153809866534 1102 34-35 1.2871064767805507 1102 36-37 -2.4861665244112885 1102 38-39 0.2223695456544874 1102 40-41 2.1065602298452717 1102 42-43 1.118556572001502 1102 44-45 1.3122610627629427 1102 46-47 1.983412235224307 1102 48-49 -3.6108749444386845 1102 50-51 -0.31568370204681884 1102 52-53 0.5713909906903112 1102 54-55 0.8725534676006248 1102 56-57 0.8933939154099875 1102 58-59 0.39601840550785994 1102 60-61 -6.079594188561622 1102 62-63 -1.7330211399584527 1102 64-65 0.40174954643028116 1102 66-67 2.0259983995586808 1102 68-69 -0.5676081465434422 1102 70-71 -0.6551984667838369 1102 72-73 -4.229042740848165 1102 74-75 -1.0501012759855612 1102 76 -0.0912152320705708 1103 1 1.6861046604202166 1103 2 0.7856101725480826 1103 3 0.3681114191799537 1103 4 0.5830789981559548 1103 5 0.4663149355339371 1103 6 0.5550164099727226 1103 7 1.0537659537849322 1103 8 0.9069441198166217 1103 9 -0.23379911612440907 1103 10-11 0.2655877023817155 1103 12-13 -0.7531179446466538 1103 14-15 -0.047486760524130034 1103 16-17 0.1776206503989286 1103 18-19 0.7854080511961712 1103 20-21 -0.3505588015271641 1103 22-23 0.7716770520996334 1103 24-25 -2.6691054058686348 1103 26-27 0.06032509329234159 1103 28-29 -0.008477586477532384 1103 30-31 1.0880517335127209 1103 32-33 1.778962898717154 1103 34-35 0.4978555902557318 1103 36-37 -0.4008385102268974 1103 38-39 -0.14861892952282574 1103 40-41 0.44734037168924345 1103 42-43 -0.6287949350907027 1103 44-45 1.4392224989331552 1103 46-47 1.4005332458626043 1103 48-49 -3.323929022452873 1103 50-51 -0.9914594112666819 1103 52-53 0.15158824246336877 1103 54-55 -0.18325326998802183 1103 56-57 0.6669865572539635 1103 58-59 -0.29143726825100913 1103 60-61 -4.454139844589989 1103 62-63 -1.0732804484690917 1103 64-65 0.06811701096928147 1103 66-67 -0.004076955051239395 1103 68-69 0.2946480591303171 1103 70-71 0.368649050946658 1103 72-73 -4.198380063543201 1103 74-75 -1.8312293788224387 1103 76 -3.254468778169862 1104 1 -0.0372225755478226 1104 2 0.592137562554818 1104 3 0.7037810061636804 1104 4 -0.0513871614666499 1104 5 -0.35303617643294416 1104 6 0.3055699865588366 1104 7 0.8869035296008576 1104 8 1.1667496459550648 1104 9 1.3528572812954422 1104 10-11 -0.323061570748159 1104 12-13 0.3011036785361725 1104 14-15 0.7250269585477795 1104 16-17 0.36636619771768864 1104 18-19 0.8696864761504415 1104 20-21 -0.19845521049992243 1104 22-23 0.1415239761392968 1104 24-25 0.970116698685132 1104 26-27 1.5198610517018878 1104 28-29 1.814522317248283 1104 30-31 0.834109305477682 1104 32-33 0.743784310096764 1104 34-35 0.022848369691566006 1104 36-37 2.0283036867500925 1104 38-39 1.8187826301190384 1104 40-41 2.133332477205755 1104 42-43 0.6844620207194438 1104 44-45 1.2951866849348903 1104 46-47 1.436077676400778 1104 48-49 0.25085676747684715 1104 50-51 0.9781275724629808 1104 52-53 0.9943147274927284 1104 54-55 0.14795882205010003 1104 56-57 0.4511687080193454 1104 58-59 -1.2315739775992327 1104 60-61 0.3212235904730676 1104 62-63 0.709958215245166 1104 64-65 0.8461664959023736 1104 66-67 0.0031147268588327393 1104 68-69 2.3712823134867307 1104 70-71 1.9325654849507004 1104 72-73 0.5932729642800432 1104 74-75 0.01325892386361005 1104 76 -0.01603612196498716 1105 1 1.7024783884498156 1105 2 0.44592469613734664 1105 3 0.554420392352938 1105 4 -0.09582941997540217 1105 5 -0.025729467796310246 1105 6 0.07842066529186553 1105 7 1.0921563330633788 1105 8 -0.8402344185737576 1105 9 1.120317442617491 1105 10-11 0.6419984331865294 1105 12-13 -0.027678536691063016 1105 14-15 0.5817454318902904 1105 16-17 0.5444106596496212 1105 18-19 1.1885532870888653 1105 20-21 0.5962034556421187 1105 22-23 0.4094661362809475 1105 24-25 1.2030500057872402 1105 26-27 0.7304453523117687 1105 28-29 1.3560922562606734 1105 30-31 1.5078944717180889 1105 32-33 1.6321081346098438 1105 34-35 -0.2920149000310417 1105 36-37 1.2012428958785648 1105 38-39 0.3044800529008569 1105 40-41 1.0790239979612153 1105 42-43 0.5777730575087432 1105 44-45 1.7635518236325112 1105 46-47 1.692855170006915 1105 48-49 0.4004928091844988 1105 50-51 1.504470283460428 1105 52-53 0.9749462443135073 1105 54-55 1.660687525571646 1105 56-57 1.0964962704824543 1105 58-59 0.8586089852180052 1105 60-61 1.2512255578151894 1105 62-63 -0.09409450952367138 1105 64-65 0.9357395826621584 1105 66-67 1.3851922401384016 1105 68-69 1.1389588824420684 1105 70-71 2.2000551564045665 1105 72-73 1.9808983823402464 1105 74-75 1.2574847747390834 1105 76 1.464310130248272 1106 1 -1.7589787208333796 1106 2 0.2706015469011547 1106 3 0.5654662207900714 1106 4 0.0993527186849974 1106 5 0.46251965088758595 1106 6 0.6297720166432228 1106 7 -1.004083384915468 1106 8 0.6879676965848844 1106 9 -0.4745121696034218 1106 10-11 0.42096435563070855 1106 12-13 1.9091017218283284 1106 14-15 -0.18405950347985822 1106 16-17 0.6593342789210652 1106 18-19 0.9001866595918031 1106 20-21 0.9443233146315535 1106 22-23 1.249595395975426 1106 24-25 0.2522861985016931 1106 26-27 1.3266850702906154 1106 28-29 1.2267150529704267 1106 30-31 1.5996101003902368 1106 32-33 0.6072550206262974 1106 34-35 1.5264927380418172 1106 36-37 1.816930322435553 1106 38-39 1.2034224735824068 1106 40-41 1.403407076692119 1106 42-43 1.5798882161456405 1106 44-45 1.8173708600602367 1106 46-47 1.6429139244134987 1106 48-49 1.9580721276333861 1106 50-51 0.3876665231784031 1106 52-53 0.9825255627623832 1106 54-55 0.5954701342672948 1106 56-57 0.6122424514460292 1106 58-59 1.176705342444798 1106 60-61 1.5882408339609029 1106 62-63 1.20849068436587 1106 64-65 -0.4208996652814321 1106 66-67 1.1354860121712917 1106 68-69 2.680674511114219 1106 70-71 0.6780222539368808 1106 72-73 0.811568182575261 1106 74-75 -0.23910746967925434 1106 76 0.48170143459609704 1107 1 1.418234271975944 1107 2 0.06251823423068714 1107 3 0.3556968183601228 1107 4 0.33121485605172296 1107 5 0.23521987404652833 1107 6 0.484842683640494 1107 7 0.7979002500956867 1107 8 0.44596422522361223 1107 9 0.15482083312407724 1107 10-11 0.8841803238925507 1107 12-13 0.30740323435002637 1107 14-15 0.7142544067606593 1107 16-17 0.8970233441330677 1107 18-19 1.3003602276552755 1107 20-21 1.1589278274012003 1107 22-23 0.7820030329702163 1107 24-25 0.5365163001629867 1107 26-27 0.48708675638037136 1107 28-29 1.5667224916017162 1107 30-31 1.025285776065914 1107 32-33 1.3640985614147922 1107 34-35 0.9057513544563989 1107 36-37 2.0607562584633285 1107 38-39 1.0065343010061625 1107 40-41 1.0798018200593376 1107 42-43 1.617242542949505 1107 44-45 1.1251194343225706 1107 46-47 1.6661100563252234 1107 48-49 1.029272226815138 1107 50-51 1.554490227616789 1107 52-53 1.6830834601092803 1107 54-55 1.5486811434416126 1107 56-57 1.414585620302958 1107 58-59 1.1403800263029709 1107 60-61 1.708225956698314 1107 62-63 1.2190287453626496 1107 64-65 1.5272034837391502 1107 66-67 0.48724648824370576 1107 68-69 2.218276792294475 1107 70-71 1.5877296677727344 1107 72-73 1.8804994992129984 1107 74-75 0.9411305665220837 1107 76 2.128490425421788 1108 1 -0.17542016227481838 1108 2 0.6298920874416964 1108 3 0.3468569865558422 1108 4 0.640991232198509 1108 5 0.2805658220587617 1108 6 0.033628998625204076 1108 7 -0.5413581596902404 1108 8 -0.7790931142259296 1108 9 0.014339181747928365 1108 10-11 0.7788190853604391 1108 12-13 1.5230234560625675 1108 14-15 1.2291865550787051 1108 16-17 0.1412177248670119 1108 18-19 0.8501022001323406 1108 20-21 1.454106535352274 1108 22-23 0.613066679759207 1108 24-25 1.7726831579611542 1108 26-27 -0.22874848826794647 1108 28-29 0.05047631423169463 1108 30-31 1.1294524427325854 1108 32-33 1.7457403809866534 1108 34-35 1.5657523101138864 1108 36-37 2.2039376422553687 1108 38-39 2.1234112123211517 1108 40-41 2.187289396511936 1108 42-43 0.6524107386681592 1108 44-45 1.9919485627629427 1108 46-47 1.663099735224307 1108 48-49 1.7198542222279798 1108 50-51 1.4603579646198455 1108 52-53 1.0193076573569826 1108 54-55 0.7032826342672962 1108 56-57 1.0652689154099946 1108 58-59 1.1486225721745242 1108 60-61 1.956864144771714 1108 62-63 2.022187193374876 1108 64-65 1.5215412130969455 1108 66-67 2.005165066225352 1108 68-69 2.2813501867898935 1108 70-71 1.0895931998828274 1108 72-73 1.1589780924851745 1108 74-75 0.8639612240144388 1108 76 2.3827431012627613 1109 1 -1.594746403409573 1109 2 0.45901442786723123 1109 3 0.5617284404565481 1109 4 0.5149938917729742 1109 5 0.08120855255521064 1109 6 -0.25881337726132614 1109 7 -0.32814893983209004 1109 8 0.7016249708804523 1109 9 1.1779030115351645 1109 10-11 0.8655877023817169 1109 12-13 0.5383714170554654 1109 14-15 1.1487898352205477 1109 16-17 1.3185780972074355 1109 18-19 1.2912591150259587 1109 20-21 1.3308241771962415 1109 22-23 0.6062515201847347 1109 24-25 0.4314265090249876 1109 26-27 0.7906442422285167 1109 28-29 -1.1786903524349768 1109 30-31 0.9800730101084696 1109 32-33 1.6241756646746026 1109 34-35 0.8058343136599859 1109 36-37 1.960863617432679 1109 38-39 1.5939342619665382 1109 40-41 0.7829786695615866 1109 42-43 1.087694426611428 1109 44-45 1.5487969670182622 1109 46-47 1.577660905437071 1109 48-49 1.2063901264832992 1109 50-51 0.3830086738397043 1109 52-53 -0.7255394171111007 1109 54-55 0.4986616236290047 1109 56-57 0.24783762108374674 1109 58-59 -0.13611811931483686 1109 60-61 1.0554346234951169 1109 62-63 0.6660812536585681 1109 64-65 1.4798191386288622 1109 66-67 0.7554975130338661 1109 68-69 -5.647068271272815E-4 1109 70-71 0.22290437009559128 1109 72-73 0.39789653220148224 1109 74-75 -0.45463363414158664 1109 76 0.5721269665109929 1110 1 -1.176764248296326 1110 2 -0.1759546867518509 1110 3 0.23660833064186448 1110 4 0.19996300639206055 1110 5 0.13906716614478398 1110 6 -0.06351481857910102 1110 7 0.4168407650409378 1110 8 0.6578020470643935 1110 9 1.251032730135023 1110 10-11 -0.8718865598008563 1110 12-13 0.6282821926217039 1110 14-15 1.3562194851862301 1110 16-17 1.0343124829315258 1110 18-19 -0.20443409019024017 1110 20-21 1.333861239653345 1110 22-23 -0.15049178798273033 1110 24-25 -0.8140607936517483 1110 26-27 1.6960835009793698 1110 28-29 0.7019548088553478 1110 30-31 -0.24084325619215008 1110 32-33 0.4600884992662273 1110 34-35 1.3286723369956093 1110 36-37 1.958255518599458 1110 38-39 1.750763362858784 1110 40-41 -1.1262354690794538 1110 42-43 0.31746450210902566 1110 44-45 1.5878154982468118 1110 46-47 0.8214666707081761 1110 48-49 2.188150593195722 1110 50-51 0.11512946999618379 1110 52-53 0.7674427380021456 1110 54-55 -0.18194922057141127 1110 56-57 0.8063139422917089 1110 58-59 -1.2451778310512864 1110 60-61 0.19582583832010414 1110 62-63 -0.8674465431842577 1110 64-65 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-2.642345415630352 2116 60-61 -2.3985093308380385 2116 62-63 0.34148343321227514 2116 64-65 -1.5632529942201288 2116 66-67 -1.095825787433185 2116 68-69 -1.4046254229662054 2116 70-71 0.33006576085843164 2116 72-73 0.26212849898923807 2116 74-75 0.12450492320142814 2116 76 -0.9451278336965849 2117 1 0.4604494029425723 2117 2 0.42563484106488403 2117 3 -0.21949265112532146 2117 4 -0.15814826055511588 2117 5 0.26267632930513685 2117 6 -0.37375324775160834 2117 7 0.6733157533532363 2117 8 0.4278815234552269 2117 9 -1.7637405283970011 2117 10-11 -1.5927841755091237 2117 12-13 -1.6276785366910573 2117 14-15 0.48609325797725234 2117 16-17 -2.1367487606402378 2117 18-19 -2.2302872926212842 2117 20-21 0.18678316578705534 2117 22-23 0.5203357014983396 2117 24-25 -2.434631153633049 2117 26-27 -3.0985401549346108 2117 28-29 -2.1859367292465706 2117 30-31 -0.38413451378915653 2117 32-33 -2.839630995824937 2117 34-35 -0.45360910292959034 2117 36-37 -2.1849889881794127 2117 38-39 -0.6071141499976918 2117 40-41 -0.8825702049373376 2117 42-43 -0.5686037540854585 2117 44-45 -0.8393467270921278 2117 46-47 -1.2796085981090215 2117 48-49 -0.5386376255981062 2117 50-51 -1.6143702962497244 2117 52-53 -0.2518653498893926 2117 54-55 -0.8697472570370479 2117 56-57 -0.600605178792911 2117 58-59 -1.223999710434171 2117 60-61 -0.9966005291413325 2117 62-63 -0.1593119008280155 2117 64-65 -0.6570140405262421 2117 66-67 -2.4162570352239214 2117 68-69 -0.8653889436448878 2117 70-71 -2.052843394320071 2117 72-73 -0.7060581393988876 2117 74-75 -1.7671529064203426 2117 76 -1.677718855258977 2118 1 -0.30290772446387493 2118 2 0.023082385949159345 2118 3 -0.5692655258819741 2118 4 0.066597575482092 2118 5 0.18393957827766627 2118 6 0.0993161876799249 2118 7 0.6281070144391094 2118 8 -0.10791774109160457 2118 9 -1.6246533555655063 2118 10-11 -1.6090074071768754 2118 12-13 -1.8531814568727611 2118 14-15 0.6869368660239772 2118 16-17 -4.73949589453597 2118 18-19 -1.3040489192706417 2118 20-21 -1.076249496986044 2118 22-23 0.2841254484159279 2118 24-25 -0.06885135696421685 2118 26-27 -3.2801321947356 2118 28-29 -3.4995858748230333 2118 30-31 -2.5232216866206514 2118 32-33 -1.1209418329436929 2118 34-35 -0.005415289388594147 2118 36-37 -4.153299608988409 2118 38-39 -1.3046049568330744 2118 40-41 -1.1384646457766152 2118 42-43 0.740719196379608 2118 44-45 -3.582911511863923 2118 46-47 -0.14030511552196145 2118 48-49 -0.2592735762794831 2118 50-51 -1.5236283537881192 2118 52-53 -3.207954469508689 2118 54-55 -2.9985597164789723 2118 56-57 -0.2412065199133977 2118 58-59 -1.7652067188702532 2118 60-61 -2.2785602955267947 2118 62-63 -2.4740425951823326 2118 64-65 -0.7469988988433514 2118 66-67 -1.1000121725806196 2118 68-69 -2.104843843060852 2118 70-71 -1.7805094120574694 2118 72-73 -0.5422034995546312 2118 74-75 -1.2926774575775966 2118 76 -1.0556119982397227 2119 1 0.06995020809555186 2119 2 -1.9962653199657119 2119 3 -0.39099023566637925 2119 4 0.3684217877540661 2119 5 0.2892463776143117 2119 6 -0.7400978532266507 2119 7 -0.34922853006061416 2119 8 -0.01173200311481537 2119 9 -0.4601978552891097 2119 10-11 -1.086342951676599 2119 12-13 -1.0455529328263253 2119 14-15 -0.8440194634398139 2119 16-17 -1.2039790343922476 2119 18-19 -0.9537172443121023 2119 20-21 -2.8719930016847712 2119 22-23 -1.1592134128333882 2119 24-25 0.3673012135167113 2119 26-27 -0.11705867345312271 2119 28-29 0.3953837216390994 2119 30-31 0.8215820723622116 2119 32-33 0.6398376032088819 2119 34-35 -1.005138893589816 2119 36-37 -1.24658319107796 2119 38-39 -2.009690639530703 2119 40-41 -2.189736066451026 2119 42-43 -1.7087003724429408 2119 44-45 -1.1336301409407596 2119 46-47 0.6000210315206047 2119 48-49 -1.1936295740683178 2119 50-51 0.5587375942494717 2119 52-53 0.1567497869866159 2119 54-55 -0.8693446805475205 2119 56-57 -2.82564543644186 2119 58-59 -0.5380093722699186 2119 60-61 -0.23613354041346923 2119 62-63 -0.2547225288473385 2119 64-65 -1.133262027643795 2119 66-67 -0.07527474858945737 2119 68-69 -0.6769831465434386 2119 70-71 -0.37047624456161543 2119 72-73 -1.0655589445518672 2119 74-75 -0.36578414635592793 2119 76 0.07429402718868872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 2.0 21 12.0 22 5.0 23 25.0 24 46.0 25 120.0 26 226.0 27 455.0 28 739.0 29 1231.0 30 1973.0 31 2967.0 32 4270.0 33 6116.0 34 8225.0 35 9889.0 36 11499.0 37 8092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.03288426099869 27.164403413665394 11.479076091818294 27.32363623351761 2 17.771456175191886 19.780652317821552 42.338038752616605 20.109852754369957 3 16.513695811640098 29.726441593759507 32.09346429785483 21.666398296745566 4 11.917413629613726 19.81464584116079 37.84910453902993 30.418835990195554 5 10.870770937326677 42.63682393144043 33.914801495715025 12.577603635517864 6 23.339237471597514 36.19415669225127 17.385003488808977 23.08160234734224 7 23.104861073837508 37.59325854758199 21.39981750845365 17.90206287012685 8 20.43189665968905 32.95761544379439 25.130159411733132 21.480328484783424 9 20.371066144239887 20.02218524681087 20.34601828493729 39.26073032401195 10-11 20.88991465836509 23.90907627073158 30.668420016817848 24.532589054085484 12-13 24.65067181936915 21.115345392088454 26.160699908754225 28.073282879788163 14-15 25.201724724026263 25.513033832501385 21.454386059077166 27.830855384395182 16-17 22.844542250371244 27.175138210509363 22.332850267475354 27.64746927164403 18-19 20.104485355947972 28.162739520154584 23.48863006100943 28.24414506288802 20-21 22.10920866655932 26.33871862308339 23.43585064319324 28.116222067164042 22-23 25.04964843540336 29.01257760363552 23.440323475211567 22.497450485749557 24-25 22.227291431842986 31.278514304116793 23.625498720770043 22.868695543270174 26-27 19.735923997638345 35.16719446084483 23.970801352584402 21.126080188932423 28-29 18.89503157819405 29.59762403163187 31.87519009536078 19.632154294813304 30-31 21.883777932835955 25.438784820997263 29.446442309412628 23.230994936754154 32-33 20.581289249100962 24.68287620990106 32.923621920455155 21.812212620542823 34-35 20.695793748769972 27.516862576709066 32.877999033868285 18.909344640652677 36-37 19.319950619934517 26.501529708550265 31.474424346519243 22.704095324995972 38-39 22.06626947918344 25.16415293507237 27.440824432397616 25.32875315334657 40-41 20.89796575599807 27.037374984345085 29.782799277190346 22.281859982466496 42-43 23.324924409138887 26.086450897250103 31.035192242320147 19.55343245129086 44-45 20.834451541337913 24.912779775642747 27.616159447515788 26.63660923550355 46-47 23.930545864419518 23.517256185926684 28.17705258261321 24.375145367040595 48-49 21.517005707333656 23.417064748716296 30.29091299447158 24.77501654947847 50-51 18.915606605478324 23.399173420643013 31.578194049344283 26.107025924534376 52-53 21.622564542966025 23.496681158642406 32.888733830712255 21.992020467679314 54-55 20.229724652460952 23.857191419319058 31.869822696938794 24.0432612312812 56-57 22.74882364517918 23.443901740826224 31.304456729823055 22.50281788417154 58-59 20.3433345857263 30.980623691696636 28.57871289785841 20.09732882471866 60-61 19.364678940117724 35.78891811139141 25.17936056393466 19.667042384556204 62-63 17.358166496699052 38.221244162954214 24.195337519904104 20.22525182044263 64-65 17.69720716368776 39.19721610935179 23.443901740826224 19.66167498613422 66-67 17.423469844166533 38.43504553342994 23.432272377578585 20.709212244824933 68-69 17.192671712021184 36.667382319789596 24.008373141538296 22.13157282665092 70-71 17.974522748823645 34.58393716565581 24.234698441665326 23.20684164385522 72-73 18.076503318841358 32.73844667489668 24.68913817472671 24.495911831535256 74-75 18.10512944375861 32.8851555650976 24.47354767144365 24.53616731970014 76 18.156119728767468 32.93077845168447 24.68287620990106 24.230225609647004 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 1.0 15 2.5 16 4.5 17 6.0 18 9.0 19 10.0 20 18.0 21 28.0 22 29.5 23 41.0 24 60.0 25 69.0 26 114.0 27 215.0 28 296.5 29 322.0 30 397.0 31 527.0 32 748.0 33 914.0 34 1015.0 35 1322.0 36 1758.0 37 1988.0 38 2676.5 39 4001.5 40 4903.0 41 4987.0 42 4806.0 43 4516.0 44 4169.5 45 3991.5 46 3870.0 47 3717.0 48 3362.5 49 2911.5 50 2662.0 51 2430.5 52 1917.5 53 1523.0 54 1410.0 55 1288.0 56 1003.5 57 719.5 58 598.0 59 472.0 60 301.0 61 193.5 62 131.0 63 103.5 64 65.5 65 39.5 66 16.5 67 9.0 68 6.0 69 3.5 70 2.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55893.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.79385611793963 #Duplication Level Percentage of deduplicated Percentage of total 1 75.36169222070708 40.53996028125167 2 11.494329331160408 12.366485964253126 3 4.889081052316493 7.89007568031775 4 2.697309342468487 5.8039468269729655 5 1.6729304553164597 4.499669010430645 6 1.147437389829381 3.7035049111695564 7 0.7483287324974225 2.8178841715420533 8 0.4589749559317524 1.97520261929043 9 0.37582731898759436 1.819548065052869 >10 1.1075265240961851 8.394611131984327 >50 0.026607243822130578 0.975077379993917 >100 0.013303621911065289 2.019930939473637 >500 0.0 0.0 >1k 0.0066518109555326445 7.194103018267047 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT 2806 5.020306657363176 No Hit CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 1215 2.17379636090387 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT 496 0.8874098724348309 No Hit CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 327 0.5850464279963502 No Hit TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT 194 0.34709176462168784 No Hit CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 112 0.20038287442076824 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 90 0.1610219526595459 No Hit CTTATAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 83 0.1484980230082479 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGTCTT 82 0.14670889020091962 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGTCTT 64 0.11450449966901044 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGTCTT 62 0.11092623405435387 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 58 0.1037697028250407 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.001789132807328288 0.0 6 0.0 0.0 0.0 0.001789132807328288 0.0 7 0.0 0.0 0.0 0.001789132807328288 0.0 8 0.0 0.0 0.0 0.003578265614656576 0.0 9 0.0 0.0 0.0 0.003578265614656576 0.0 10 0.0 0.0 0.0 0.0053673984219848635 0.0 11 0.0 0.0 0.0 0.0053673984219848635 0.0 12 0.0 0.0 0.0 0.00894566403664144 0.0 13 0.0 0.0 0.0 0.00894566403664144 0.0 14 0.0 0.0 0.0 0.00894566403664144 0.0 15 0.0 0.0 0.0 0.00894566403664144 0.0 16 0.0 0.0 0.0 0.00894566403664144 0.0 17 0.0 0.0 0.0 0.00894566403664144 0.0 18 0.0 0.0 0.0 0.010734796843969727 0.0 19 0.0 0.0 0.0 0.019680460880611168 0.0 20 0.0 0.0 0.0 0.023258726495267744 0.0 21 0.0 0.0 0.0 0.02683699210992432 0.0 22 0.0 0.0 0.0 0.02683699210992432 0.0 23 0.0 0.0 0.0 0.02683699210992432 0.0 24 0.0 0.0 0.0 0.028626124917252607 0.0 25 0.0 0.0 0.0 0.030415257724580896 0.0 26 0.0 0.0 0.0 0.030415257724580896 0.0 27 0.0 0.0 0.0 0.03578265614656576 0.0 28 0.0 0.0 0.0 0.03757178895389405 0.0 29 0.0 0.0 0.0 0.0447283201832072 0.0 30 0.0 0.0 0.0 0.05009571860519206 0.0 31 0.0 0.0 0.0 0.05725224983450521 0.0 32 0.0 0.0 0.0 0.06798704667847494 0.0 33 0.0 0.0 0.0 0.0751435779077881 0.0 34 0.0 0.0 0.0 0.09303490598107098 0.0 35 0.0 0.0 0.0 0.11450449966901043 0.0 36 0.0 0.0 0.0 0.15386542143023277 0.0 37 0.0 0.0 0.0 0.21827420249405113 0.0 38 0.0 0.0 0.0 0.2808938507505412 0.0 39 0.0 0.0 0.0 0.4204462097221477 0.0 40 0.0 0.0 0.0 0.5582094358864258 0.0 41 0.0 0.0 0.0 0.7245987869679567 0.0 42 0.0 0.0 0.0 0.9446621222693361 0.0 43 0.0 0.0 0.0 1.1897733168733116 0.0 44 0.0 0.0 0.0 1.4617215035872113 0.0 45 0.0 0.0 0.0 1.6907305029252322 0.0 46 0.0 0.0 0.0 1.9662569552537885 0.0 47 0.0 0.0 0.0 2.2489399388116578 0.0 48 0.0 0.0 0.0 2.504785930259603 0.0 49 0.0 0.0 0.0 2.7659993201295334 0.0 50 0.0 0.0 0.0 3.0290018428067915 0.0 51 0.0 0.0 0.0 3.3206304904013026 0.0 52 0.0 0.0 0.0 3.578265614656576 0.0 53 0.0 0.0 0.0 3.8555811997924607 0.0 54 0.0 0.0 0.0 4.122161988084375 0.0 55 0.0 0.0 0.0 4.431681963752169 0.0 56 0.0 0.0 0.0 4.67142575993416 0.0 57 0.0 0.0 0.0 4.957687009106686 0.0 58 0.0 0.0 0.0 5.215322133361959 0.0 59 0.0 0.0 0.0 5.4854811872685305 0.0 60 0.0 0.0 0.0 5.725224983450522 0.0 61 0.0 0.0 0.0 5.982860107705795 0.0 62 0.0 0.0 0.0 6.258386560034351 0.0 63 0.0 0.0 0.0 6.5052868874456555 0.0 64 0.0 0.0 0.0 6.759343746086272 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTT 15 0.0022138075 70.0 7 GGAATGA 15 0.0022138075 70.0 40 CTATGCG 15 0.0022138075 70.0 41 TTAATAC 25 2.8057384E-6 70.0 2 GCTATGC 15 0.0022138075 70.0 40 CGTCTGC 15 0.0022138075 70.0 38 AAGAAGC 15 0.0022138075 70.0 58 GTTGTCT 15 0.0022138075 70.0 49 GAGGTGC 15 0.0022138075 70.0 35 TTGCGGC 15 0.0022138075 70.0 24 CTACAGC 15 0.0022138075 70.0 65 CTTTAGG 15 0.0022138075 70.0 34 TACAGCG 15 0.0022138075 70.0 66 AGGTGCT 15 0.0022138075 70.0 36 TTTACAC 215 0.0 66.74419 2 CTTTACA 235 0.0 59.57447 1 GGTTTAA 25 2.3713263E-4 55.999996 10 AGACTTC 20 0.006917629 52.5 11 GACAGTT 20 0.006917629 52.5 26 GTACATG 20 0.006917629 52.5 1 >>END_MODULE