FastQCFastQC Report
Wed 25 May 2016
SRR1780399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985500
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC84150.8538812785388128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT11150.035.6828143
CAACTCG250.002361187735.1738142
TCGGTGG13400.031.33487742
GGTGGTC12450.031.24907944
CAATGTG12300.029.14609529
ACGAGAC12800.028.91368321
CGAGACC13000.028.4370422
CTCGGTG14700.027.96699141
CTTAGAC406.9971534E-427.5228441
AATGTGT13150.027.09487330
TCTCGGT15400.026.71881940
CCCAATG13750.026.55236627
CTCGCTA1107.2759576E-1226.02159713
GACCCAA14350.025.15095725
ACCCAAT14450.025.11388826
CCAATGT14600.024.85587128
CGACGAG18000.024.72070719
GTAGATC16250.024.64418235
ACGGTGC450.001388401124.47345715
AGATCTC16550.024.33041437
Produced by FastQC (version 0.11.5)