FastQCFastQC Report
Wed 25 May 2016
SRR1780399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985500
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT365473.708472856418062No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT356193.6143074581430747RNA PCR Primer, Index 43 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC116921.1864028411973617No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC56450.5728056823947235No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG40390.40984271943176054No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT31190.3164890918315576RNA PCR Primer, Index 43 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC20030.2032470826991375No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC18670.18944698122780315No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG13670.13871131405377982No Hit
CTTAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC11660.11831557584982243No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG11430.11598173515981736No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC10550.10705225773718924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA10650.040.0719727
TCTTCTG10600.039.84593220
CTGCTTG10900.039.35471324
CGTCTTC11250.038.13034418
TTGAAAA11400.037.62862828
GTACACG301.3015611E-436.66378820
TACACGC301.3015611E-436.66378821
CGGGTAG250.002352316635.2008140
GCGGGTA250.002352316635.2008139
GTCTTCT12400.034.5940619
TCTGCTT12700.034.46973823
CTTCTGC12650.034.43208321
TTTACAC16400.034.0774152
CCGTCTT47700.033.8540344
TGTACAC401.8181832E-533.0041123
CTTTACA17100.032.834431
TGAAAAA13200.032.4974529
CTTTATA3400.032.356972
TTCTGCT13600.032.026922
GCTTGAA13200.031.83083726
Produced by FastQC (version 0.11.5)