FastQCFastQC Report
Wed 25 May 2016
SRR1780398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8388
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT231927.646638054363375Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT84510.073915116833572No Hit
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC7839.334763948497853No Hit
CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC2953.5169289461134956No Hit
TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT1852.20553171196948No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1051.251788268955651No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG750.8941344778254651No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTCTT640.7629947544110633No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC610.7272293752980448No Hit
CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC600.7153075822603719No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGTCTT580.6914639961850262No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT520.619933237958989No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC500.5960896518836434No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGTCTT470.5603242727706247No Hit
ATACACATCTCCGAGCCCACGAGACTTCCCTATATCTCGTATGCCGTCTT460.5484024797329518Illumina PCR Primer Index 6 (95% over 22bp)
CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG450.5364806866952789No Hit
TTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC450.5364806866952789No Hit
CTTATCACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC400.4768717215069146No Hit
CTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT390.46494992846924177No Hit
ATACACATCTCCGAGCCCACGAGAATTCCATATATCTCGTATGCCGTCTT320.3814973772055317TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTCCATATCTCTCGTATGCCGTCTT280.33381020505484027No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT280.33381020505484027No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGT250.2980448259418217No Hit
TCTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT240.28612303290414876No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG240.28612303290414876No Hit
TCTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT220.26227944682880305No Hit
ATACACATCTCCGAGCCCACGAGCCTTCCATATATCTCGTATGCCGTCTT220.26227944682880305Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTCAATATATCTCGTATGCCGTCTT210.2503576537911302Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCCT210.2503576537911302No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG200.2384358607534573No Hit
CTTATACACATCTCCGAGCCCACGCGACTTCCATATATCTCGTATGCCGT200.2384358607534573No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTCTGCCGTCTT190.22651406771578447No Hit
ACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCT180.2145922746781116Illumina PCR Primer Index 2 (95% over 23bp)
ATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTGCTT170.20267048164043872Illumina PCR Primer Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTTCCATCTATCTCGTATGCCGTCTT160.19074868860276584Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCAGTCTT160.19074868860276584No Hit
CTTATATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC140.16690510252742013No Hit
CTTTACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTC140.16690510252742013No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC130.15498330948974726No Hit
ATACACATCTCCGAGCCCACGAGACTTACATATATCTCGTATGCCGTCTT120.14306151645207438TruSeq Adapter, Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGTC120.14306151645207438No Hit
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGTC120.14306151645207438No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTATT110.13113972341440153No Hit
CTTATACACATCTACGAGCCCACGAGACTTCCATATATCTCGTATGCCGT110.13113972341440153No Hit
CTTATACACATCTCCGAGCCCACGAGAATTCCATATATCTCGTATGCCGT110.13113972341440153No Hit
CTTATACACATCTCCGAGCCCACGAGCCTTCCATATATCTCGTATGCCGT110.13113972341440153No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCAGT110.13113972341440153No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCACGTATGCCGT100.11921793037672865No Hit
ATACACATATCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT100.11921793037672865Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGCCTT100.11921793037672865No Hit
ATACACATCTCCGAGCACACGAGACTTCCATATATCTCGTATGCCGTCTT100.11921793037672865TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGATTTCCATATATCTCGTATGCCGTCTT100.11921793037672865TruSeq Adapter, Index 2 (95% over 21bp)
ATACACATCTACGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT100.11921793037672865Illumina PCR Primer Index 2 (95% over 21bp)
CTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTCTGCTTGA100.11921793037672865Illumina PCR Primer Index 2 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGTC100.11921793037672865No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCCCGTATGCCGT100.11921793037672865No Hit
ATACACATCTCAGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT100.11921793037672865Illumina PCR Primer Index 2 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCAT90.1072961373390558No Hit
TACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTTC90.1072961373390558Illumina PCR Primer Index 2 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGCCTTCCATATATCTCGTATGCCGTC90.1072961373390558No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGACGT90.1072961373390558No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCCTATATCTCGTATGCCGT90.1072961373390558No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTAGTATGCCGT90.1072961373390558No Hit
CTTATACACATCTCCGAGCCCACGAGACTTACATATATCTCGTATGCCGT90.1072961373390558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT150.00214814370.01
TTTACAC1100.066.818182
CTTTACA1050.066.666671
TCTTATA409.054929E-961.251
TTACACA1250.058.83
CTATATC252.2557733E-455.99999630
CCTATAT252.2557733E-455.99999629
ACTTCCC252.2557733E-455.99999624
CTTCCCT252.2557733E-455.99999625
CAACAAA200.006713985552.569
ACGAGAA200.006713985552.519
CTTATAC1600.052.51
CAAAACC200.006713985552.570
TTCCCTA305.548728E-446.66666426
TAATACA451.4923826E-646.6666643
CCCTATA305.548728E-446.66666428
TCCCTAT305.548728E-446.66666427
TTATACA1850.045.4054072
CTTAATA404.21317E-543.751
TTAATAC404.21317E-543.752
Produced by FastQC (version 0.11.5)