FastQCFastQC Report
Wed 25 May 2016
SRR1780397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91973
Sequences flagged as poor quality0
Sequence length50
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1670.18157502745370924No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC1360.14786948343535603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCC207.85641E-443.9570432
CTATACA452.3084795E-839.1154671
TTTACAC1550.038.2851642
CTTTACA1450.036.4178471
GCCGACA250.002321540135.26150520
TTATAAC353.213326E-431.3978862
TATACCC353.213326E-431.3978863
ATACCCA353.213326E-431.3978864
TTACACA1850.030.8887333
CCGACAA300.005684289629.36857421
GAGTGTT300.00569946129.35257739
AGTGTTT300.00569946129.35257740
AAAAAGC300.00571466429.33661
CTTATCC454.0261686E-529.33661
GAGTAAA300.005745165529.30469530
TAAGACA300.005745165529.3046953
TTATACA6850.028.5560342
TATACAC7250.027.8899863
TGTTATT406.955702E-427.51804236
TTAATAC407.0224865E-427.473152
Produced by FastQC (version 0.11.5)