##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780397_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 91973 Sequences flagged as poor quality 0 Sequence length 50 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.326258793341523 16.0 10.0 26.0 7.0 30.0 2 18.301414545573156 16.0 10.0 27.0 7.0 31.0 3 18.97846107009666 16.0 10.0 28.0 7.0 31.0 4 20.578071825427028 19.0 10.0 32.0 7.0 35.0 5 20.662911941548064 19.0 10.0 32.0 7.0 35.0 6 20.827536342187383 17.0 10.0 32.0 8.0 35.0 7 20.69983582138236 17.0 10.0 32.0 8.0 35.0 8 20.8377132419297 17.0 10.0 32.0 8.0 35.0 9 21.088547725963053 17.0 10.0 32.0 8.0 37.0 10 20.91439879094952 17.0 10.0 32.0 7.0 37.0 11 21.060909179868005 17.0 10.0 32.0 7.0 37.0 12 20.879910408489447 17.0 10.0 32.0 7.0 37.0 13 20.843290965826927 17.0 10.0 32.0 7.0 37.0 14 21.04549161166864 17.0 10.0 32.0 7.0 37.0 15 20.765213704021832 17.0 10.0 31.0 7.0 37.0 16 20.625727115566526 17.0 9.0 31.0 7.0 37.0 17 20.73320430996053 17.0 10.0 31.0 7.0 37.0 18 20.38587411522947 17.0 8.0 31.0 7.0 37.0 19 20.444858817261586 18.0 8.0 31.0 7.0 37.0 20 20.560283996390243 18.0 8.0 31.0 7.0 37.0 21 20.290074260924403 18.0 8.0 31.0 7.0 37.0 22 20.374609939873658 18.0 8.0 31.0 7.0 37.0 23 20.219684037706717 18.0 8.0 30.0 7.0 37.0 24 19.68235242951736 17.0 8.0 30.0 7.0 37.0 25 19.60163308797147 17.0 8.0 30.0 7.0 37.0 26 19.23689561066835 16.0 8.0 30.0 7.0 37.0 27 19.040685853456992 16.0 8.0 30.0 7.0 36.0 28 18.881443467104475 16.0 8.0 28.0 7.0 36.0 29 18.665727985387015 15.0 8.0 28.0 7.0 36.0 30 18.465179998477815 15.0 8.0 28.0 7.0 35.0 31 18.35765931305927 15.0 8.0 28.0 7.0 35.0 32 18.02548574038033 15.0 8.0 27.0 7.0 35.0 33 18.03231383123308 15.0 8.0 27.0 7.0 35.0 34 17.718352125080187 15.0 8.0 27.0 7.0 35.0 35 17.612690680960718 15.0 8.0 26.0 7.0 35.0 36 17.483750665956315 14.0 8.0 26.0 7.0 34.0 37 17.3216161264719 14.0 8.0 26.0 7.0 34.0 38 17.33404368673415 13.0 8.0 26.0 7.0 34.0 39 17.01106846574538 13.0 8.0 25.0 7.0 34.0 40 16.519293705761473 13.0 8.0 24.0 7.0 33.0 41 16.61227751622759 13.0 8.0 25.0 7.0 33.0 42 16.6528763876355 13.0 8.0 24.0 7.0 33.0 43 16.691996564209063 13.0 8.0 25.0 7.0 33.0 44 16.667924282126275 13.0 8.0 25.0 7.0 33.0 45 16.720439694258097 13.0 8.0 25.0 7.0 33.0 46 16.59083644112946 13.0 8.0 24.0 7.0 33.0 47 16.513052743740012 13.0 8.0 24.0 7.0 33.0 48 16.404934056734042 13.0 8.0 24.0 7.0 33.0 49 16.13492003087863 12.0 8.0 24.0 7.0 32.0 50 16.110456329574983 12.0 7.0 24.0 7.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.19195427309898605 1101 2 0.19826593559074723 1101 3 0.18738679226391852 1101 4 -0.5277354037616142 1101 5 -0.43951774561087475 1101 6 0.29592332064138205 1101 7 -0.03265719339752593 1101 8 -0.09350908538620573 1101 9 2.0382432533746737 1101 10 0.8867128703213609 1101 11 1.1388177186604587 1101 12 1.3632639380720732 1101 13 0.3324797458351547 1101 14 -0.35264467687873946 1101 15 1.2555365610690004 1101 16 1.1750719332260218 1101 17 0.6640489369426419 1101 18 -0.15495785987683774 1101 19 0.18235064434878367 1101 20 1.9775424143003448 1101 21 0.7242823266727818 1101 22 0.8743076582436693 1101 23 0.18742226391794858 1101 24 0.7392087984615081 1101 25 0.07628216701349189 1101 26 -0.01898301510900069 1101 27 -0.5057326428276028 1101 28 -1.0013134210742365 1101 29 1.0093543303984163 1101 30 0.46738324331342795 1101 31 0.7328616662957579 1101 32 0.6837992880077302 1101 33 -0.03643616582888498 1101 34 -0.2387022383890134 1101 35 -0.29325567325200197 1101 36 -0.5676269883457614 1101 37 0.06790526603761649 1101 38 0.46999848648556863 1101 39 -0.35678537931976706 1101 40 -1.1236953983949558 1101 41 -0.4749427822107144 1101 42 0.013981536525758287 1101 43 -0.5270440878423024 1101 44 0.20449807957562172 1101 45 -0.05174544345863907 1101 46 0.46522464962296084 1101 47 0.41328421901985024 1101 48 0.22751357140995765 1101 49 -0.15781367783568 1101 50 0.11232007144404932 1102 1 0.3615099931312358 1102 2 0.2895097981112045 1102 3 0.2718112330713396 1102 4 -0.9891374768658174 1102 5 -0.6030474782895432 1102 6 -0.5535038479347243 1102 7 -0.19727803355028328 1102 8 -0.053820051016320036 1102 9 0.0779322877445594 1102 10 -0.42520747883303045 1102 11 -0.9809313266204995 1102 12 -0.44674697300266786 1102 13 -0.9109736093203296 1102 14 -0.07495782472380341 1102 15 -0.7256418350030103 1102 16 0.47608120763954886 1102 17 -0.023757937034442733 1102 18 -0.6299987764071133 1102 19 -0.6134758695628335 1102 20 -0.3176021574399712 1102 21 -1.302449806442329 1102 22 -0.36996402751737634 1102 23 -0.12245225872253229 1102 24 -1.2718386647136128 1102 25 -0.4845470746668141 1102 26 0.12777094015559243 1102 27 -0.1886295331713015 1102 28 0.40867021231365186 1102 29 -0.2346445784941089 1102 30 0.32035651118031083 1102 31 -0.9278039092634351 1102 32 -0.4494522122650473 1102 33 0.18383115550226847 1102 34 0.3852748483540296 1102 35 -0.9200969171145204 1102 36 -1.5212549206970962 1102 37 -0.7740205386541454 1102 38 -1.3760189712340143 1102 39 -0.7644504093252209 1102 40 -0.007628840839036144 1102 41 -0.3789253244911315 1102 42 -0.4486480324867905 1102 43 -0.3787898598008397 1102 44 -1.4613502564854794 1102 45 -1.0149205662082288 1102 46 -0.6126531463399427 1102 47 -0.6506819566484516 1102 48 -0.6554651519942976 1102 49 0.05467950274260858 1102 50 -0.27829640427880875 1103 1 -0.28008726311569987 1103 2 0.12002588499671774 1103 3 0.0021313375952658475 1103 4 -0.31867365194339214 1103 5 -0.31262611621212955 1103 6 -0.5878007612125273 1103 7 -1.1239071369309777 1103 8 -1.7852017266655125 1103 9 -1.311623485569175 1103 10 -0.7789148118397264 1103 11 -1.154130738673416 1103 12 -1.0103187515425986 1103 13 -0.4966750628561769 1103 14 0.02107353245405008 1103 15 -1.242137017103289 1103 16 -1.0477224833615928 1103 17 -1.2458304505082332 1103 18 -1.2724534554859979 1103 19 -0.051782255321448645 1103 20 -0.026568350482595093 1103 21 -0.3155152972148265 1103 22 -0.6699395297221784 1103 23 -1.1172260624135326 1103 24 -0.8678291922328043 1103 25 -1.0782103116421524 1103 26 -1.232020011992045 1103 27 -0.22696858265684838 1103 28 -0.3323880924389222 1103 29 -1.0084890991539162 1103 30 -0.8247520957117338 1103 31 -2.580098536080353 1103 32 -0.9941606885021876 1103 33 -0.6267600579551882 1103 34 -0.8066653770256842 1103 35 -0.8417039724795394 1103 36 -0.06837234679541382 1103 37 -0.8800551622046946 1103 38 -0.0767784028851679 1103 39 0.2650694338888897 1103 40 -0.2779620108537344 1103 41 0.3520888842300849 1103 42 -0.8580878495699196 1103 43 -1.2599347234297813 1103 44 -0.7810464838250191 1103 45 -0.9999769111371855 1103 46 -0.40797406745704023 1103 47 -0.24814235187954736 1103 48 -0.9983526254550199 1103 49 -1.029837890691983 1103 50 -0.7679256709781388 1104 1 -0.06491141155103719 1104 2 0.04699506646603879 1104 3 0.031446525315324436 1104 4 -0.012031249759587581 1104 5 -0.6839122656760708 1104 6 -0.9505415927985545 1104 7 -0.3115876369903212 1104 8 -0.582606481976665 1104 9 -0.6351191535677501 1104 10 -0.6156438540520135 1104 11 -0.9869036152014843 1104 12 -0.45546651433090446 1104 13 -1.158027280287044 1104 14 -0.4390285366206008 1104 15 -1.3806028160509491 1104 16 -0.7300423789188173 1104 17 0.4089940935436722 1104 18 -0.6506628948973194 1104 19 -0.8747834026094985 1104 20 0.30638255350126187 1104 21 0.13074019196506015 1104 22 0.558726846825671 1104 23 0.6670046611691696 1104 24 -0.792662044232646 1104 25 -0.14309142886334314 1104 26 -0.5519551308748234 1104 27 -0.5618975694828166 1104 28 -0.4405892239023075 1104 29 -0.11332748978571772 1104 30 -0.6604525348461259 1104 31 -1.108246654923569 1104 32 -0.9438382609989233 1104 33 -0.68768500949216 1104 34 -0.3890920890563834 1104 35 0.18262644647840887 1104 36 0.27075017565582726 1104 37 -0.744437802549669 1104 38 -1.1095354547504996 1104 39 -1.1298350247098377 1104 40 -1.4375141728982825 1104 41 -0.9699430024653299 1104 42 -0.4822521095691279 1104 43 0.008174624989731427 1104 44 -0.4300554643211214 1104 45 -0.9172139250233258 1104 46 -0.9811433517866703 1104 47 -1.178672082464665 1104 48 -0.04780469650775743 1104 49 -0.14622828512170116 1104 50 -1.2743148785581528 1105 1 1.206083228115432 1105 2 0.28327516516424467 1105 3 0.747136136478435 1105 4 1.3216282414341478 1105 5 1.2739282856725218 1105 6 1.2879081647101671 1105 7 -0.26023904514143226 1105 8 0.7082979679510153 1105 9 0.5204156035155485 1105 10 1.3372617870608927 1105 11 0.1349801591539972 1105 12 0.40125091951577474 1105 13 0.13389818555146604 1105 14 0.12525292343215 1105 15 0.44488854785613796 1105 16 0.04712089854225354 1105 17 0.42592594073162715 1105 18 0.984068663002791 1105 19 1.0176270457866572 1105 20 1.1144315622649827 1105 21 0.3996927998098663 1105 22 1.2530602085205587 1105 23 -0.027966835469985796 1105 24 -0.5204336770072544 1105 25 -0.02529171815118403 1105 26 0.2570298091404908 1105 27 1.2030986017075165 1105 28 0.0556670510913122 1105 29 0.3539574587380656 1105 30 1.017757283923551 1105 31 1.4222136530828955 1105 32 0.13405674453158056 1105 33 0.07854488925709546 1105 34 -0.05755971434354379 1105 35 0.06567758235860666 1105 36 0.33473383940125245 1105 37 0.7155298652798194 1105 38 0.11159957460370862 1105 39 0.9044115506326271 1105 40 0.878830590140943 1105 41 1.4998944858355294 1105 42 1.2966979457359002 1105 43 0.9458501120994036 1105 44 0.6819584895629944 1105 45 1.1463017730963045 1105 46 1.0425101834809212 1105 47 0.5206387317955254 1105 48 1.0249001448093544 1105 49 1.538873391046085 1105 50 1.4868950252961817 1106 1 1.1817611705252702 1106 2 0.9458415615858264 1106 3 1.4626798882257077 1106 4 1.4437510631655819 1106 5 0.8842628304493374 1106 6 0.8986154613274451 1106 7 0.49882348397980536 1106 8 1.1736786407680846 1106 9 0.965294925107198 1106 10 1.5733534840136478 1106 11 2.39818955250038 1106 12 1.8615994424866074 1106 13 0.9978437637827575 1106 14 1.2332577692060767 1106 15 1.1914172963418324 1106 16 0.15726906739883972 1106 17 1.4305853387374015 1106 18 1.0080179687524868 1106 19 1.3479317704162348 1106 20 1.177426623303095 1106 21 2.156472223908274 1106 22 1.5202766841519058 1106 23 1.099421106007977 1106 24 0.6882607596715253 1106 25 1.4395764208852526 1106 26 1.9671220652210053 1106 27 0.7377179288904472 1106 28 0.1917680668400763 1106 29 1.1697248615896143 1106 30 1.1165365216460366 1106 31 1.3150009049699527 1106 32 0.22859593006880985 1106 33 0.6080854726137268 1106 34 0.9717478991127955 1106 35 1.040656615067583 1106 36 1.2003568009146264 1106 37 0.4220809788759432 1106 38 0.951689035045419 1106 39 0.9245867439976436 1106 40 0.22353285460837213 1106 41 0.9617340161682115 1106 42 0.7182802772986641 1106 43 0.6828270946732573 1106 44 0.6320563369211136 1106 45 0.9570836200816721 1106 46 1.2090370682074152 1106 47 -0.03699802153594334 1106 48 0.5112015146723685 1106 49 0.38408818929415034 1106 50 1.0880039620215562 1107 1 -0.5985586006953199 1107 2 -0.33857284111793007 1107 3 -0.13799605093976908 1107 4 0.1526639276744426 1107 5 -0.758485988823594 1107 6 -0.7661447102571124 1107 7 -0.07295172291824059 1107 8 -0.7852017266655125 1107 9 -1.1778655237857336 1107 10 -0.26299124496074455 1107 11 0.5146590702437805 1107 12 0.12343921024084281 1107 13 -0.5412610501173241 1107 14 -0.7623659579918112 1107 15 -0.20392045659373537 1107 16 -0.8821173878201911 1107 17 -0.10570306197319823 1107 18 -0.08774007968981934 1107 19 -0.4976421279329166 1107 20 -0.8673326816927869 1107 21 -0.5894006475332958 1107 22 -0.5234427144355536 1107 23 -0.9388821133689476 1107 24 -0.6703769629334388 1107 25 -1.4221593562281392 1107 26 -0.8817015406544648 1107 27 -1.1441660348861475 1107 28 -1.1030887293815965 1107 29 -0.9702725386443625 1107 30 -0.2706119683231982 1107 31 -0.9176781539147498 1107 32 -1.6884282044257546 1107 33 -1.3210275738787551 1107 34 -1.2397863961339652 1107 35 -0.05826448203367818 1107 36 -0.25308572259159234 1107 37 -0.4978895571091506 1107 38 -2.3888803137131926 1107 39 -1.4483063622894559 1107 40 -2.0805097815543725 1107 41 -2.14472640239412 1107 42 -2.0236929451113195 1107 43 -2.6930557425380606 1107 44 -1.8829573118504968 1107 45 -1.4076202232390962 1107 46 -1.4207129209602272 1107 47 -2.088906683089739 1107 48 -1.5397538993403703 1107 49 -2.3292009480168243 1107 50 -1.487670893908076 1108 1 -0.6679605363392938 1108 2 -0.4038801452787375 1108 3 -0.9250752138151093 1108 4 -1.284758522486861 1108 5 -0.5198806451227078 1108 6 0.5878547934239187 1108 7 -0.6325927188649665 1108 8 -0.26386001105628054 1108 9 -0.381025421213149 1108 10 -1.1066094102349595 1108 11 0.03455318886401315 1108 12 -0.2976460739017668 1108 13 0.3416903933436721 1108 14 0.8850821353161571 1108 15 0.3729262635650876 1108 16 -0.45590347434513134 1108 17 -0.10342826670477479 1108 18 -1.7746041710125091 1108 19 -0.10290310899007338 1108 20 -0.5343853742231879 1108 21 -0.5550305590230806 1108 22 -0.3492347567881069 1108 23 -0.1590822953525688 1108 24 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1.3730476261914362 2211 8 0.9075780399438145 2211 9 0.618405804495449 2211 10 1.0573397792616746 2211 11 2.2136222949404214 2211 12 0.5688341611916066 2211 13 1.9733142439456266 2211 14 1.3669287558489067 2211 15 1.2576072759833252 2211 16 0.35569004162794826 2211 17 0.1619523380918899 2211 18 0.35133193667542173 2211 19 0.9446843400572291 2211 20 0.3309099144297676 2211 21 0.7922535437354732 2211 22 0.6652689876426443 2211 23 1.026527715074959 2211 24 -0.14892082829212683 2211 25 1.5220035523217703 2211 26 1.1588396281585887 2211 27 1.5804608654811396 2211 28 1.6756550522911908 2211 29 1.0527875757263665 2211 30 0.6039986597859652 2211 31 1.0633995344947529 2211 32 1.6882513250735265 2211 33 1.461156628083213 2211 34 1.323652164468573 2211 35 1.3146026898478596 2211 36 1.8878785249326988 2211 37 0.5201494033260161 2211 38 1.7215385807031005 2211 39 0.6584861431733735 2211 40 1.615756897510206 2211 41 1.6019842768564274 2211 42 1.232804335096965 2211 43 1.3471599811125046 2211 44 1.9229843869732512 2211 45 1.5429121120624067 2211 46 0.4270717273265383 2211 47 1.1465761380549004 2211 48 2.197332901533681 2211 49 1.529974280957095 2211 50 -0.21578470673061467 2212 1 -0.40952769401383193 2212 2 0.06308577470731791 2212 3 -0.06793235667225161 2212 4 -0.4243035412265108 2212 5 0.1944486497434994 2212 6 0.3974512372485499 2212 7 0.7984628027945817 2212 8 0.7434179458861081 2212 9 0.5686825888975449 2212 10 0.2709291585787774 2212 11 0.2562911824140883 2212 12 0.13959789018783297 2212 13 -0.2754977876028981 2212 14 0.1361934401187348 2212 15 0.8960239712265512 2212 16 0.957022525829192 2212 17 -0.41263248530496455 2212 18 0.9945494941143096 2212 19 0.5858443851265456 2212 20 0.24718875634423298 2212 21 -0.1255439382612913 2212 22 -0.9560507595772627 2212 23 0.798731702729885 2212 24 1.0167089174581285 2212 25 2.827103243694914 2212 26 1.9560276945868438 2212 27 1.0941531204167205 2212 28 1.0826505943477258 2212 29 0.7012958792584278 2212 30 0.9074327459075526 2212 31 1.4941544455808256 2212 32 1.0715854748800204 2212 33 1.719668360802558 2212 34 1.2305252355507257 2212 35 1.729877269772416 2212 36 1.332987034216 2212 37 1.4107583290106263 2212 38 1.3966965682600474 2212 39 0.9976817538136515 2212 40 0.28831850041031615 2212 41 1.8198356460819198 2212 42 0.8196362559317265 2212 43 1.0801242685763732 2212 44 1.4604360109383734 2212 45 -0.25265933750404557 2212 46 0.1461233121009471 2212 47 0.561458810861442 2212 48 0.46422164890055484 2212 49 2.4096098073373433 2212 50 1.8431973145303466 2213 1 0.3138393539113018 2213 2 -0.2419997721307574 2213 3 -0.2862422158271798 2213 4 0.04833092327864108 2213 5 0.1198669312445837 2213 6 1.4210356970273565 2213 7 0.9346287920185148 2213 8 1.2530596889620114 2213 9 0.3965491169247706 2213 10 0.8596426475663925 2213 11 0.854929995156315 2213 12 1.03727252862247 2213 13 0.9367150869599179 2213 14 1.4808384916358932 2213 15 1.0085582372252304 2213 16 1.4712960866572473 2213 17 2.3150827996936165 2213 18 1.314475763137846 2213 19 1.7414356508416446 2213 20 1.40287454895223 2213 21 0.8786625084728037 2213 22 0.7099384643569451 2213 23 1.3600323927762048 2213 24 1.7632823175904662 2213 25 1.1131416216134333 2213 26 1.781436523398643 2213 27 0.6653791342176483 2213 28 1.8582910140471292 2213 29 1.7647234243950187 2213 30 1.3199953699741016 2213 31 1.8802495396350842 2213 32 1.6862465959689708 2213 33 1.3576303609916138 2213 34 0.7365749566963942 2213 35 0.8805531370566584 2213 36 1.8137035480641899 2213 37 1.0503745498254489 2213 38 0.4779896961549355 2213 39 1.2715734259943865 2213 40 1.3106268471349622 2213 41 0.7142491067114705 2213 42 1.2481739248985484 2213 43 1.337804578624585 2213 44 0.9660603610659848 2213 45 1.2255994771287178 2213 46 0.9444974495430465 2213 47 0.5339514850272451 2213 48 1.2647226414329324 2213 49 1.2995332405540907 2213 50 1.2654016816727989 2214 1 -0.7888396572184142 2214 2 -0.4523316937302866 2214 3 -0.20079948953938498 2214 4 0.19675995903162047 2214 5 1.2588406335985702 2214 6 0.0688737744428991 2214 7 -0.1046206908929399 2214 8 0.89548064828438 2214 9 1.001591961404234 2214 10 0.5742097705842184 2214 11 -0.14526699095616635 2214 12 1.3172390409833454 2214 13 0.8961359477892294 2214 14 1.3166505668845865 2214 15 0.4153838060226285 2214 16 0.9242164057747502 2214 17 0.9570112937347872 2214 18 1.4976236012152633 2214 19 1.3836103775234108 2214 20 0.5565237166859056 2214 21 0.20570870171617983 2214 22 0.26814786059451023 2214 23 1.5582004219301488 2214 24 1.248557605682656 2214 25 0.8348002446805047 2214 26 0.9692627816474371 2214 27 0.7781536836119152 2214 28 0.18139748504092523 2214 29 0.10393351008398 2214 30 -0.34281365198985014 2214 31 0.6199816385221126 2214 32 -0.6330186458314806 2214 33 0.18674490841602065 2214 34 0.08282729590647975 2214 35 1.0309520339344296 2214 36 0.8050854056432293 2214 37 0.3967253820917733 2214 38 1.0662887210736756 2214 39 0.366668471793659 2214 40 1.3262872430770472 2214 41 0.9847110391452318 2214 42 0.4231468393080817 2214 43 0.42132669031570913 2214 44 1.1575308623956388 2214 45 1.4571074058197446 2214 46 1.2352489515621556 2214 47 1.3102737543072607 2214 48 0.5461665496374017 2214 49 0.18405013211323862 2214 50 0.8848737588913522 2215 1 0.3919347922127514 2215 2 0.023819021226398007 2215 3 -0.29927564025433995 2215 4 -1.0770632327020202 2215 5 -0.17853542318047388 2215 6 0.18874643909168398 2215 7 -0.24521144342399026 2215 8 -0.13447447122297973 2215 9 -0.17187636131782114 2215 10 0.0427412391156885 2215 11 0.43870127957926996 2215 12 0.3336778260788478 2215 13 1.2533011706260915 2215 14 0.5765921178709164 2215 15 0.43834591853698157 2215 16 -0.0596057422265055 2215 17 -0.21003807480711956 2215 18 -0.08493563059770182 2215 19 0.9231678349913182 2215 20 0.6582876244199625 2215 21 0.7059696348129343 2215 22 -0.898953694566746 2215 23 -0.9810439158216511 2215 24 0.523320156564612 2215 25 0.03420531721990727 2215 26 -0.10567720374030998 2215 27 0.045871040881511504 2215 28 -0.7061403502581065 2215 29 0.9508701555280865 2215 30 -0.05124700967694196 2215 31 -0.8977618120571584 2215 32 0.18057074984746535 2215 33 0.10757780686085994 2215 34 -0.3538181482016505 2215 35 -0.1944941616610194 2215 36 0.49175694306103424 2215 37 0.7874987877905539 2215 38 1.0972875729680531 2215 39 0.6537470648424026 2215 40 0.012750034017450673 2215 41 0.763439773097847 2215 42 0.11258426755147966 2215 43 -0.03942897193249095 2215 44 0.3761927974754862 2215 45 0.23421835457248363 2215 46 -0.33624671694843755 2215 47 -0.5713669930051299 2215 48 -1.1231268435365873 2215 49 -1.1847005202195042 2215 50 -1.6053151566706951 2216 1 0.9501852068064487 2216 2 0.8333132169146253 2216 3 0.5570676433277484 2216 4 1.3014608137324757 2216 5 1.2569952567531928 2216 6 0.7424363230054496 2216 7 1.4191749579027082 2216 8 1.105367983171714 2216 9 0.514898099710372 2216 10 1.7510745724490206 2216 11 1.3406498699606963 2216 12 1.433594907339213 2216 13 0.7033426299959089 2216 14 0.2658541410881625 2216 15 0.9762812419200664 2216 16 0.5583461649045063 2216 17 1.9719685586920512 2216 18 1.9212832748749378 2216 19 1.8640882330012687 2216 20 2.91637220153439 2216 21 1.326823697831177 2216 22 1.4783265707732198 2216 23 0.6147272284569567 2216 24 2.477092959218009 2216 25 1.4214358313160922 2216 26 2.473924786309439 2216 27 2.38936191982015 2216 28 2.7847385883820266 2216 29 4.257876789027257 2216 30 2.427194118017919 2216 31 3.411939680480156 2216 32 3.4479836851708505 2216 33 2.865455087126197 2216 34 2.7645483526275356 2216 35 1.0125953905777862 2216 36 2.4424202929781167 2216 37 3.425765786132178 2216 38 3.9241947039796585 2216 39 3.147942753067941 2216 40 3.2445079112977133 2216 41 1.86252766696186 2216 42 4.033189574350818 2216 43 2.64257766156668 2216 44 1.596342051206829 2216 45 2.4423443910567286 2216 46 1.383073349386553 2216 47 3.0619248809583866 2216 48 2.038979292450147 2216 49 2.174337622400728 2216 50 2.099908723926319 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 4.0 5 5.0 6 20.0 7 599.0 8 3254.0 9 6826.0 10 6546.0 11 5231.0 12 4934.0 13 4959.0 14 4936.0 15 4689.0 16 4361.0 17 4197.0 18 3667.0 19 3361.0 20 2985.0 21 2823.0 22 2646.0 23 2417.0 24 2342.0 25 2239.0 26 1987.0 27 2004.0 28 1771.0 29 1663.0 30 1465.0 31 1393.0 32 1416.0 33 1271.0 34 1119.0 35 988.0 36 843.0 37 729.0 38 655.0 39 1628.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.71816024901504 38.446050368951475 7.977623473585687 25.858165908447795 2 18.96849820036319 23.157139284277374 31.450692126181178 26.423670389178255 3 19.947592175794544 23.90753607115286 32.91146122147199 23.233410531580606 4 14.938079653811446 35.043980298565884 23.200287040761964 26.81765300686071 5 15.85791482282844 42.20151566220521 25.057353788611874 16.88321572635447 6 32.49431898491949 31.7560588433562 13.215835082034946 22.533787089689366 7 26.954649734161112 31.126526263142445 17.99332412773314 23.925499874963304 8 23.17636697726507 28.9552368629924 18.415187065769302 29.453209093973232 9 28.83735090408933 20.747844429222255 13.827184656032879 36.58762001065553 10 22.93657783431734 25.945134879472874 28.237161714018548 22.881125572191234 11 32.1014232747279 20.74458253145013 19.933457285448675 27.220536908373294 12 24.102162638222918 23.152950386534886 25.27100933990062 27.473877635341577 13 28.25578740200289 17.733534854893605 22.651603292485348 31.359074450618156 14 36.598997466482544 18.937227483771352 17.468222297128317 26.995552752617787 15 27.20214859677928 23.688931899485684 20.421455522089445 28.68746398164559 16 33.637432503048245 19.212680717645007 19.968211113046507 27.181675666260237 17 40.978829362706186 18.566442310828883 16.39718594713321 24.057542379331718 18 35.252237148642465 16.665940908794006 17.908075507849073 30.17374643471445 19 31.649842649156625 17.684274716060674 20.61786068188995 30.04802195289275 20 36.013334640651046 17.397130437624604 21.731978080639713 24.857556841084637 21 28.904045411459638 19.97319765098112 17.712430406502293 33.41032653105695 22 35.57924507777657 16.30806048080061 15.775046230827805 32.33764821059501 23 42.97070401705126 18.29095891602688 16.182387611735795 22.555949455186063 24 30.751314228496174 28.059730732811634 15.292939627118274 25.89601541157392 25 39.366515837104075 19.84315175774452 14.987600069613643 25.802732335537765 26 35.42450675425812 26.155619847295036 15.547845380783537 22.87202801766331 27 31.85160209697418 25.751016945465622 19.31978856235453 23.077592395205674 28 39.81032345072107 21.968329236725904 16.71524590520523 21.506101407347792 29 33.727797778962135 22.084208007483223 20.727873916401094 23.460120297153548 30 39.43403000663671 19.83070947526465 18.20525932131472 22.530001196783918 31 35.420609541212556 19.212947856163936 22.089886662750985 23.276555939872523 32 31.707927253741737 23.01927427775844 20.9993908806126 24.273407587887224 33 36.29006819887532 19.977810892241425 22.524119777673842 21.208001131209414 34 31.464499777001816 21.842944011139032 22.420563695895744 24.271992515963404 35 34.7538558593835 21.735299876003396 21.30239944310296 22.20844482151015 36 37.38035154890358 18.579229028889664 22.761486251305254 21.278933170901496 37 32.319288246938285 19.311087424680775 25.59765939396576 22.771964934415177 38 35.17299890142163 17.1965585128947 24.360159674559753 23.27028291112392 39 32.22346709016349 18.51565814234279 27.913806794078294 21.347067973415424 40 26.19348375294015 30.368498998170573 23.01267532014984 20.42534192873944 41 30.59009082503943 15.37390547669549 28.638712133572632 25.39729156469245 42 24.528199271224235 18.639255996084188 34.169793876108116 22.662750856583454 43 24.921416995682012 16.92389684689094 30.940494447526127 27.214191709900916 44 25.343396883054737 15.403103894550242 35.81659398144624 23.436905240948786 45 24.984229874056513 18.05733800274074 34.78454745176517 22.173884671437584 46 24.491593622898407 18.98340474585119 30.36627009156752 26.158731539682883 47 29.23250932582192 21.224809405213758 28.76485878041088 20.777822488553436 48 29.410229361290252 21.190634142097423 24.857804700329524 24.541331796282805 49 27.247023000380622 23.698548202925345 25.633190147354686 23.42123864933935 50 28.148212596113066 22.483115640191844 24.77569087211389 24.592980891581202 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.0 5 1.5 6 1.0 7 1.0 8 1.0 9 0.5 10 0.0 11 5.0 12 10.0 13 10.0 14 10.0 15 15.5 16 21.0 17 24.5 18 28.0 19 36.0 20 44.0 21 58.5 22 73.0 23 105.5 24 138.0 25 173.5 26 209.0 27 276.5 28 344.0 29 428.0 30 512.0 31 652.5 32 793.0 33 963.5 34 1134.0 35 1345.0 36 1556.0 37 1903.5 38 2251.0 39 2401.5 40 2552.0 41 2898.5 42 3245.0 43 3580.5 44 3916.0 45 4326.5 46 4737.0 47 5048.5 48 5360.0 49 5775.5 50 6191.0 51 6472.5 52 6754.0 53 6816.0 54 6878.0 55 6739.5 56 6601.0 57 6413.0 58 6225.0 59 5830.5 60 5436.0 61 5060.0 62 4684.0 63 4348.5 64 4013.0 65 3719.0 66 3425.0 67 3130.0 68 2835.0 69 2661.0 70 2487.0 71 2360.0 72 2233.0 73 2067.5 74 1902.0 75 1691.0 76 1480.0 77 1360.0 78 1240.0 79 1073.0 80 906.0 81 773.0 82 640.0 83 550.5 84 461.0 85 375.0 86 289.0 87 211.0 88 133.0 89 116.0 90 99.0 91 77.0 92 55.0 93 43.5 94 32.0 95 21.0 96 10.0 97 10.5 98 11.0 99 13.5 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0989420808280691 2 0.010872756134952649 3 0.0021745512269905295 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0021745512269905295 10 0.0021745512269905295 11 0.0021745512269905295 12 0.0021745512269905295 13 0.00652365368097159 14 0.00652365368097159 15 0.00652365368097159 16 0.127211246778946 17 0.00652365368097159 18 0.12503669555195546 19 0.1522185858893371 20 0.1978841616561382 21 0.20658236656410034 22 0.04675285138029639 23 0.016309134202428976 24 0.10220390766855489 25 0.0402291976993248 26 0.03370554401835321 27 0.03370554401835321 28 0.029356441564372154 29 0.036967370858839006 30 0.0652365368097159 31 0.03805464647233427 32 0.0402291976993248 33 0.039141922085829534 34 0.04784012699379166 35 0.03805464647233427 36 0.0402291976993248 37 0.03370554401835321 38 0.039141922085829534 39 0.043491024539810595 40 0.15330586150283235 41 0.04131647331282007 42 0.04131647331282007 43 0.03479281963184847 44 0.02609461472388636 45 0.029356441564372154 46 0.020658236656410034 47 0.02609461472388636 48 0.02392006349689583 49 0.019570961042914767 50 0.02609461472388636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 91973.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.28992204233852 #Duplication Level Percentage of deduplicated Percentage of total 1 98.58285505642337 93.9395257303774 2 0.7382389520886343 1.406934643862873 3 0.21907554683310324 0.6262707533732725 4 0.10155064410492806 0.3870701184043143 5 0.06732008991225567 0.32074630598110315 6 0.06161499754681028 0.35227729877246583 7 0.053627868235186726 0.35771367683994215 8 0.03993564655811777 0.3044371717786742 9 0.026243424881048825 0.22506605199351984 >10 0.10155064410492806 1.3775782022985006 >50 0.005705092365445396 0.37293553542887586 >100 0.0022820369461781583 0.32944451088906523 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 167 0.18157502745370924 No Hit ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC 136 0.14786948343535603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007610929294466854 0.0 2 0.0 0.0 0.0 0.009785480521457383 0.0 3 0.0 0.0 0.0 0.010872756134952649 0.0 4 0.0 0.0 0.0 0.015221858588933709 0.0 5 0.0 0.0 0.0 0.02609461472388636 0.0 6 0.0 0.0 0.0 0.02609461472388636 0.0 7 0.0 0.0 0.0 0.028269165950876887 0.0 8 0.0 0.0 0.0 0.030443717177867417 0.0 9 0.0 0.0 0.0 0.036967370858839006 0.0 10 0.0 0.0 0.0 0.0402291976993248 0.0 11 0.0 0.0 0.0 0.05653833190175377 0.0 12 0.0 0.0 0.0 0.05653833190175377 0.0 13 0.0 0.0 0.0 0.05871288312874431 0.0 14 0.0 0.0 0.0 0.06306198558272537 0.0 15 0.0 0.0 0.0 0.06414926119622064 0.0 16 0.0 0.0 0.0 0.06414926119622064 0.0 17 0.0 0.0 0.0 0.06741108803670642 0.0 18 0.0 0.0 0.0 0.07937111978515433 0.0 19 0.0 0.0 0.0 0.08915660030661172 0.0 20 0.0 0.0 0.0 0.10546573450904069 0.0 21 0.0 0.0 0.0 0.10546573450904069 0.0 22 0.0 0.0 0.0 0.12829852239244127 0.0 23 0.0 0.0 0.0 0.13808400291389863 0.0 24 0.0 0.0 0.0 0.13917127852739392 0.0 25 0.0 0.0 0.0 0.14025855414088917 0.0 26 0.0 0.0 0.0 0.14134582975438445 0.0 27 0.0 0.0 0.0 0.15765496395681342 0.0 28 0.0 0.0 0.0 0.17287682254574713 0.0 29 0.0 0.0 0.0 0.19462233481565241 0.0 30 0.0 0.0 0.0 0.20984419340458613 0.0 31 0.0 0.0 0.0 0.2228915007665293 0.0 32 0.0 0.0 0.0 0.24463701303643462 0.0 33 0.0 0.0 0.0 0.27181890337381626 0.0 34 0.0 0.0 0.0 0.3250954084350842 0.0 35 0.0 0.0 0.0 0.38054646472334275 0.0 36 0.0 0.0 0.001087275613495265 0.48492492361888817 0.0 37 0.0 0.0 0.001087275613495265 0.6230089265327868 0.0 38 0.0 0.0 0.001087275613495265 0.8045839539864961 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATCCC 20 7.85641E-4 43.957043 2 CTATACA 45 2.3084795E-8 39.115467 1 TTTACAC 155 0.0 38.285164 2 CTTTACA 145 0.0 36.417847 1 GCCGACA 25 0.0023215401 35.261505 20 TTATAAC 35 3.213326E-4 31.397886 2 TATACCC 35 3.213326E-4 31.397886 3 ATACCCA 35 3.213326E-4 31.397886 4 TTACACA 185 0.0 30.888733 3 CCGACAA 30 0.0056842896 29.368574 21 GAGTGTT 30 0.005699461 29.352577 39 AGTGTTT 30 0.005699461 29.352577 40 AAAAAGC 30 0.005714664 29.3366 1 CTTATCC 45 4.0261686E-5 29.3366 1 GAGTAAA 30 0.0057451655 29.304695 30 TAAGACA 30 0.0057451655 29.304695 3 TTATACA 685 0.0 28.556034 2 TATACAC 725 0.0 27.889986 3 TGTTATT 40 6.955702E-4 27.518042 36 TTAATAC 40 7.0224865E-4 27.47315 2 >>END_MODULE